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In Vitro Culture Technology for the Production of Secondary Metabolites and the Development of Plants That are Resistant to Climate Change Sutini; Widiwurjani; Augustien, Nora; Guniarti; Pribadi, Didik Huswo; Purwanto, Djoko Agus; Muslihatin, Wirda
Nusantara Science and Technology Proceedings Seminar Nasional Magister Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0604

Climate change that we are experiencing today is triggered by global warming which causes air temperature to rise, drought-land degradation, fluctuating rainfall, floods, landslides, pollution, and rising sea levels. In the agricultural sector, this results in decreased soil fertility, decreased agricultural yields, physiological effects on plants, weed disturbances and attacks by pests. Therefore, the estimation strategy and the provision of adaptive technology include applying plant-invitro culture technology that can adapt to climate change. The problem of this paper is the decrease in productivity and the stagnation of secondary metabolite biomass production in plants. This problem can be overcome by adapting in vitro culture techniques to produce secondary metabolites and plant development. In vitro culture has advantages including it does not depend on climate, altitude, weather and environmental temperature. The aim of this study was to adapt the in vitro plant culture techniques to obtain secondary metabolites and adaptive plant development. The method used is a review of the literature related to in vitro culture techniques that can produce secondary metabolite biomass and the development of various plant varieties. The conclusion of this research is that in vitro culture techniques can produce a variety of secondary metabolites and adaptive plants. The implication of this paper is to obtain a variety of secondary metabolite biomass and various plants that are resistant to climate change.

The Role of In Vitro Cultute Methods of Cammelia sinensis L. Plants in the Global Society Transformation Sutini; Widiwuriani; Guniarti; Agus Purwanto
Nusantara Science and Technology Proceedings 4th International Seminar of Research Month
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2019.0439

The purpose of this paper is to explore the role of Cammelia sinensis L in vitro culture in the transformation of a global society with all its changes, both in positive terms and weaknesses. What makes this study relevant to this goal is the transformation of the global society, besides obtaining benefits, it also raises various problems. Problems that arise include the explosion of the world population due to climate change, the threat of malnutrition, the emergence of obesity disorders, disruption of agricultural production and chaos or the high cost of transportation whether by land, sea, or air. The method used is examining the literature on the role of in vitro culture in global social transformation related to climate change, health, agriculture, and transportation. The results obtained from the study of the role of in vitro culture in this global society transformation include obtaining some seeds with certain clones that are resistant to climate change so that nutritional deficiencies can be anticipated, secondary metabolites obtained from Cammelia sinensis L can be applied to reduce obesity. And in vitro culture can facilitate transportation because of the packaging form model from practical-efficient. This study concludes that the in vitro culture method of Cammelia sinensis L is very important in reducing problems in the global society transformation.

Production of Sweet Corn (Zea mays saccharata Sturt) Bonanza Variety on Planting Distance Setting and The Use of NPK Fertilizer Carolina Rosa Siallagan; Sutini Sutini; Didik Utomo Pribadi; Ramadhani Mahendra Kusuma
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1503

Sweet corn (Zea mays saccharata Sturt) cultivation can be done through variety selection technology use, fertilization, and the proper planting distance. One of the techniques used in sweet corn cultivation is planting distance and fertilization with NPK fertilizer. So, this study is critical in order to set the planting distance and NPK fertilizer dose to enhance sweet corn production. The goal of this study was to see how different planting distances and NPK fertilizer doses affected sweet corn production. The study was carried out in a rice field in Sambiroto Village, Padas District, at an elevation of ± 60 meters above sea level. It was conducted during January-May 2019. The method used was a factorial experiment with three replications based on a Randomized Block Design and two factors. The first factor is the planting distance, they are J1: 60 cm x 15 cm, J2: 60 cm x 20 cm, J3: 60 cm x 25 cm. The second factor is the NPK fertilizer dose, they are P0 = 0 kg / ha, P1 = 100 kg / ha, P2 = 200 kg / ha, P3 = 300 kg / ha. The results showed that (1) the J2 (60 x 20 cm) planting distance had the best effect on sweet corn cobs fresh weight per plant as well as per hectare without husks and sweet corn sugar content. (2) The NPK fertilizer dose of 300 kg/ha and planting distance of 60 x 20 cm resulted in optimal production on sugar content parameters. In conclusion, planting distance setting of 60 x 20 cm and NPK fertilizer dose of 300 kg / ha resulted in optimal production for sweet corn plant.

The Role of in Vitro Culture Producing Secondary Metabolites and Plant Conservation Techniques to Overcome the Environmental Crisis Sutini; Widiwurjani; Didik Utomo Pribadi; Nora Augustien
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3108

This paper aims to examine the role of in vitro culture as a technique to produce secondary metabolites that are needed in various fields. In addition to producing secondary metabolites, in vitro culture can also be used as a plant conservation technique that can produce plantlet asexually in large numbers. This large number of plantlets besides being commercialized, its growth and development of plants is useful for overcoming the decline in environmental quality. An example of a plant will be useful as the lungs of an area and its roots will reduce the erosion of land areas. The conceptual framework of this study is that in addition to producing secondary metabolites, in vitro culture also has a function for plant conservation which in the future can prevent environmental damage. The problem in this study is the instability in the secondary metabolite biomass production and the need for optimization in the domain of conservation techniques. The method of this paper writing was extracting data information from journals, books and online sources which being analysed descriptively and qualitatively to verify the data objectively. The result of this study showed the role of in vitro culture that can produce secondary metabolites and can be implemented in plant conservation that overcome environmental crises.

Production of Sweet Corn (Zea mays saccharata Sturt) Bonanza Variety on Planting Distance Setting and The Use of NPK Fertilizer Carolina Rosa Siallagan; Sutini Sutini; Didik Utomo Pribadi; Ramadhani Mahendra Kusuma
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1503

Sweet corn (Zea mays saccharata Sturt) cultivation can be done through variety selection technology use, fertilization, and the proper planting distance. One of the techniques used in sweet corn cultivation is planting distance and fertilization with NPK fertilizer. So, this study is critical in order to set the planting distance and NPK fertilizer dose to enhance sweet corn production. The goal of this study was to see how different planting distances and NPK fertilizer doses affected sweet corn production. The study was carried out in a rice field in Sambiroto Village, Padas District, at an elevation of ± 60 meters above sea level. It was conducted during January-May 2019. The method used was a factorial experiment with three replications based on a Randomized Block Design and two factors. The first factor is the planting distance, they are J1: 60 cm x 15 cm, J2: 60 cm x 20 cm, J3: 60 cm x 25 cm. The second factor is the NPK fertilizer dose, they are P0 = 0 kg / ha, P1 = 100 kg / ha, P2 = 200 kg / ha, P3 = 300 kg / ha. The results showed that (1) the J2 (60 x 20 cm) planting distance had the best effect on sweet corn cobs fresh weight per plant as well as per hectare without husks and sweet corn sugar content. (2) The NPK fertilizer dose of 300 kg/ha and planting distance of 60 x 20 cm resulted in optimal production on sugar content parameters. In conclusion, planting distance setting of 60 x 20 cm and NPK fertilizer dose of 300 kg / ha resulted in optimal production for sweet corn plant.

The Role of in Vitro Culture Producing Secondary Metabolites and Plant Conservation Techniques to Overcome the Environmental Crisis Sutini; Widiwurjani; Didik Utomo Pribadi; Nora Augustien
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3108

This paper aims to examine the role of in vitro culture as a technique to produce secondary metabolites that are needed in various fields. In addition to producing secondary metabolites, in vitro culture can also be used as a plant conservation technique that can produce plantlet asexually in large numbers. This large number of plantlets besides being commercialized, its growth and development of plants is useful for overcoming the decline in environmental quality. An example of a plant will be useful as the lungs of an area and its roots will reduce the erosion of land areas. The conceptual framework of this study is that in addition to producing secondary metabolites, in vitro culture also has a function for plant conservation which in the future can prevent environmental damage. The problem in this study is the instability in the secondary metabolite biomass production and the need for optimization in the domain of conservation techniques. The method of this paper writing was extracting data information from journals, books and online sources which being analysed descriptively and qualitatively to verify the data objectively. The result of this study showed the role of in vitro culture that can produce secondary metabolites and can be implemented in plant conservation that overcome environmental crises.

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