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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Dasar Indonesian Journal of Agricultural Science Indonesian Journal of Biotechnology Biopropal Industri Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Annales Bogorienses
Enny Sudarmonowati
Research Center for Biotechnology, Indonesian Institute of Sciences, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Economics, Econometrics & Finance Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology

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Journal : ANNALES BOGORIENSES

 1 
Massive In Vitro Propagation Of Sandalwood Through Friable Embryogenic Callus Supatmi, Supatmi; Ardiyanti, Nurdiya; Rahman, Nurhamidar; Sudarmonowati, Enny
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.302 KB) | DOI: 10.14203/ab.v20i1.219

Abstract

Sandalwood (Santalum album), which belongs to Santalaceae family, is a commercially important tree in Indonesia due to its many application. However,its population has significantly depleted since the planting materials using conventional methods are difficult to be provided. This study was conducted to mass propagate sandalwood using in vitro methods through friable embryogenic callus (FEC). The somatic embryos were formed using leaves cultured in MS +0.5 mg/l +1 mg/l indole acetic acid (IAA), and MS +1 mg/l IAA + 0.2 mg/l kinetin as well as 0.5 MS+1 mg/l Gibberellic acid (GA3). Primary somatic embryos (PSE) and secondary somatic embryos (SSE) then formed friable embryogenic callus when they were repetitively transferred to MS +1.5 mg/l BAP + 1.2 mg/l kinetin every 3 weeks. The maturation and regeneration of FEC was best done in the MS +1.5 mg/l BAP + 1.2 mg/l kinetin for 4-8 weeks. The acclimatization of sandalwood plantlets can be best conducted in the medium containing soil, sand and compos in ratio of 1:1:1 with the companion plant Murraya paniculata, which gave the best percentage of survival rate and the lowest percentage of falling leaves.
Improved Regeneration, Acclimatization and Shoot Cutting Production of “Gebang” Cassava Derived from Irradiated In Vitro Shoots Supatmi, Supatmi; Sudarmonowati, Enny
ANNALES BOGORIENSES Vol 16, No 2 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3748.807 KB) | DOI: 10.1234/64

Abstract

Gebang is an Indonesian local genotype which has been selected as superior genotype for low amylose  cassava. Prior to induction of new mutants of this genotype, series of research have been conducted to improve regeneration and acclimatization as well as shoot cuttings production of irradiated in vitro shoots. Four dosage treatments of gamma ray irradiation i.e. those of at 0; 0.2; 1.0; 1.5; 2.0 krad were applied to 32 in vitro shoots multiplied from apical shoots of plants in the field. The highest multiplication rate and acclimatized shoots were obtained from shoots irradiated with 0.2 krad multiplied on various level of BAP added on MS medium and resulted that MS supplemented with 1 mg/L BAP was the best medium. The phenotypic variation was observed in shoots irradiated with 1.0; 1.5 and 2.0 krad while the ones irradiated with 0.2 krad performed normal appearances. Subsequent production of propagated young stem cuttings called “ratooning system” decreased after the second cycle of propagation especially in the survival rate of the ones irradiated with 0.2 krad. The findings lead to the opportunity to produce cassava propagules derived from irradiated in vitro culture at a higher amount using Gebang genotype as a model.Keywords: cassava Gebang, irradiated shoot, young stem cutting/ratooning , acclimatization, in vitro.
Identification of Differentially Expressed cDNA in Cassava under Drought Stress Using cDNA-RAPD Approach Hartati, N Sri; Supatmi, Supatmi; Aryaningrum, Pramesti Dwi; Sudarmonowati, Enny
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/72

Abstract

Cassava is an important carbohydrate source that provides food security and alternative renewable energy development. This plant is naturally drought tolerant, but there is a wide variation within cassava genotypes in their ability to maintain high yield and starch production under drought stress. It has been reported that cassava clones with leaf retention or stay green trait can produce more total fresh biomass and high root dry matter compared to drought susceptible cultivars. The genetic, biochemical and molecular bases of stay green trait need to be understood to develop drought resistant cassava cultivars since water stress limits yield and starch production. Differential Display (DD)RT-PCR is a powerful technique for analyzing differences in gene expression. The method is based on the detection of the differentially expressed cDNAs from two or more samples. The main objective of this study was to identify differentially expressed cDNA in cassava under drought stress by employing a cDNA-RAPD approach. In this study, we used cassava genotype Ubi Kuning which was considered the most responsive to water insufficiency (45 days without watering). Leaf samples were collected from water stressed and well-watered plants at day 45. Among 11 random primers, OPB03 and OPH17 have identified differentially expressed cDNA in Ubi Kuning. Further characterization of these PCR products of expressed cDNA under drought stress may open possibility the development of cassava with improved drought resistance through genetic engineering and/or marker assisted selection (MAS).
Co-Authors Agus Purwito Ardiyanti, Nurdiya Ardiyanti, Nurdiya Aryaningrum, Pramesti Dwi Aryaningrum, Pramesti Dwi Didy Soepandi, Didy Didy Sopandie Fauzy Rachman Hani Fitriani, Hani Hartati Hartati Hartati, H. Hartati, N Sri I. Fitryatmi Kurnia Sofyan laela Sari, laela N. Sri Hartati, N. Sri N. Sri i Hartati Nurul Khumaida Partomuan Simanjuntak RAGAPADMI PURNAMANINGSIH Rahman, Nurhamidar Rahman, Nurhamidar S. Sadjad Sintho Wahyuning Ardie Siti Kurniawati Sri Hartati Sri N. Hartati, Sri N. Suharsono S, Suharsono Supatmi Supatmi