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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Dasar Indonesian Journal of Agricultural Science Indonesian Journal of Biotechnology Biopropal Industri Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Annales Bogorienses

Enny Sudarmonowati

Research Center for Biotechnology, Indonesian Institute of Sciences, Bogor, Indonesia

Author-ID : 387707

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Economics, Econometrics & Finance Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology

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Regeneration Rate of Eggplant Somatic Embryogenic In Various Maturation Media Hartati, H.; Hartati, N. Sri; Sudarmonowati, Enny
Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Ralstonia solanacearum is one of the most important pathogen that causes bacterial wilt disease in eggplant and inhibits eggplant production. Improvement of eggplant varieties resistant to bacterial wilt can be accomplished through genetic manipulation. Regeneration of in vitro plants isone of the important tools to supports plant improvementthrough biotechnology. This study was aimed to determine the rate of eggplant regeneration in various maturation media, and to find the best medium for eggplant regeneration based on maturation rate and the number of cotyledon produced. We used resistant eggplant (accession 032) as the material to produce somatic embryogenic.There were 7 types of regeneration media used in this research. MS medium was supplemented with a certain concentration of plant growt regulators , such as: 1 mg / L + BAP 1 mg / L, NAA 4mg / L, TDZ 0.005 mg / L, TDZ 0.001 mg / L, CuSO4 2mM + BAP 1 mg / L, CuSO4 2mM + BAP 2 mg / L and Kinetin 1 mg / L + CuSO4 2mM. Three clumps of callus per plate with three replications were transferred to MS suplemented medium. The parameters observed were the color of callus before and after they were transfered to regeneration medium, the day of formation of globular, heart-shaped, tubular and cotyledonary phase, and the number of cotyledons formed. The results obtained showed the somatic embryogenic color of the 032 genotype was white with friable structure before being transferred to regeneration medium and was turned to yellowish white after being transferred to the regeneration medium. On the day sixth, friable embryogenic somatic of eggplant was developed into nodule on medium MS + NAA 4 mg / L, MS + CuSO4 2mM + BAP medium 1 mg / L, and MS + CuSO4 2mM + BAP 2 mg / L. Somatic embryogenic callus of accession 032 were able to pass complete globular, heart-shaped, tubular and cotyledonary phase. The most responsive medium for somatic embryogenic callus regeneration, based on the days of the callus phases formation and the number of early-phase cotyledons obtained, were MS medium suplemented with CuSO4 2mM + BAP,and CuSO4 2 mM + BAP 2 mg / L.Keywords: eggplant, Ralstonia solanacearum, regeneration, cotyledonary, clump, BAP

Evaluation of Adaptation and Production of Three Selected Cassava (Manihot esculenta Crantz) in Peat Land Area of Central Kalimantan Fitriani, Hani; Hartati, N. Sri; Sudarmonowati, Enny
Jurnal ILMU DASAR Vol 20 No 2 (2019)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Along with the increasing the of the land-use change of the fertile agricultural land in Java, the better awareness has been subjected to the importance of function peat land for agricultural development. Cassava (Manihot esculenta Crantz) is a tuber plant that potentially developed as important carbohydrate source. The objective of this research was to identify superior cassava variety candidate of selected cassava developed by of Research Centre Biotechnology, LIPI which has high adaptation capability and yield in Central Kalimantan peat land area. This research was conducted from July 2010 through May 2011 in Central Kalimantan Province (Kalampangan dan Pulang Pisau in total of area 1.5 and 2.25 ha respectively). There were three genotypes of cassava tested, i.e. Iding (high amylose), Gebang (low amylose), and Menti (high starch) compared to Adira 4 (35 ton/ha) and Darul Hidayah (102.1 ton/ha). The variables observed were plant height (cm) and tuber weight (grams). Observations were conducted at the age of 10 months. Data was analyzed using SPSS version 16.0. The results showed that the plant height and yield were higher in Pulang Pisau than Kalampangan even though the difference is not significant (P ≤ 0,05). Darul Hidayah has highest plant height and yield, 229.8 cm and 2271 g respectively among other genotypes/varieties grown at Pulang Pisau even though the value was not significant, whereas in Kalampangan, Darul Hidayah showed the lowest yield (670 g). Based on the data obtained, the Darul Hidayah variety can be cultivated by farmers in Pulang Pisau peat land to fulfill the availability of superior cassava seedlings for food, feed and industry. Keywords: Peat, tuber yield, plant height, liquid organic fertilizer, yam wood.

Pola Akumulasi Prolin dan Poliamin Beberapa Aksesi Tanaman Terung pada Cekaman Kekeringan Kurniawati, Siti; Khumaida, Nurul; Ardie, Sintho Wahyuning; Hartati, N. Sri i; Sudarmonowati, Enny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 2 (2014): Jurnal agronomi indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACT Drought stress is one of the major limiting factors for plant growth and production. Therefore, the mechanism of drought tolerance is important to be studied. Eggplant (Solanum spp.) is relatively tolerant to drought stress compared to other member of Solanaceae family. The objective of this study was to study the mechanism of drought tolerance in eggplant related to accumulation pattern of proline and polyamines. Seven eggplant accessions were drought stressed for 21 days and rewatered for the following next 7 days. Drought stress treatment significantly decreased the soil water content and reduced plant height of all accessions. Eggplant responded the drought stress by reducing stomatal density and stomatal opening to reduce transpiration rate. Drought stress also induced proline and polyamine (PA) accumulation in the leaf tissues. These accumulations started at 14-days after drought stress period when soil water content less than 20% and reached their peaks at 21 days after drought stress. The highest level of proline (134.70 μmol g-1) and that of putrescine (20.836 ng g-1) could be used as indicators for eggplant drought stress condition. Keywords: physiological responses, polyamine (PA), proline, putrescine, stomata

Enhancement of Yield, Starch, and Amylose Content of Two Indonesian Cassava Genotypes by Producing Gamma Irradiated-Induced Mutants Sudarmonowati, Enny; Hartati, N. Sri; Supatmi, Supatmi
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
Publisher : BRIN

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As an important carbohydrate-source food crop and raw materials of starch-based industries, accelerated cassava breeding has been extensively pursued through various procedures, among others, is mutant induction using gamma irradiation combined with molecular marker. Iding, a local genotype cassava has been compared with a national variety Adira 4 for its yield attributes following gamma irradiation at various dose levels ( 2, 20, 30, and 50 krad). The mutation rate was conducted based on morphological characteristics and identification using RAPD by employing random primer OPB-10. Evaluation was conducted on mutant lines producing high potential yield, high starch and amylose contents. The fourth generation of irradiated Adira 4 (2 and 30 krad) and Iding (2 krad) exhibited higher yield and amylose content as compared to their representative controls. The yield of 30 krad irradiated Adira 4 indicated by mean tuber weight per individual could reach more than twice of that of control and almost 1.5 times of that irradiated with 2 krad. The levels of amylose content of all irradiated ones, regardless of genotypes and gamma irradiation doses (2 and 30 krad) were higher than that of control. In most parameters, irradiated Adira 4 was superior to irradiated Iding.

Improved Regeneration, Acclimatization and Shoot Cutting Production of “Gebang” Cassava Derived from Irradiated In Vitro Shoots Supatmi, Supatmi; Sudarmonowati, Enny
Annales Bogorienses Vol. 16 No. 2 (2012): Annales Bogorienses
Publisher : BRIN

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Gebang is an Indonesian local genotype which has been selected as superior genotype for low amylose cassava. Prior to induction of new mutants of this genotype, series of research have been conducted to improve regeneration and acclimatization as well as shoot cuttings production of irradiated in vitro shoots. Four dosage treatments of gamma ray irradiation i.e. 0; 0.2; 1.0; 1.5; 2.0 krad were applied to 32 in vitro shoots multiplied from apical shoots of plants in the field. The highest multiplication rate and acclimatized shoots were obtained from shoots irradiated with 0.2 krad multiplied on various level of BAP added on MS medium and resulted that MS supplemented with 1 mg/L BAP was the best medium. The phenotypic variation was observed in shoots irradiated with 1.0; 1.5 and 2.0 krad while that irradiated with 0.2 krad performed normal appearances. Subsequent production of propagated young stem cuttings so called “ratooning system” decreased after the second cycle of propagation especially in the survival rate of that irradiated with 0.2 krad. The findings lead to the opportunity to produce cassava propagules derived from irradiated in vitro culture at a higher amount using Gebang genotype as a model.

Identification of Differentially Expressed cDNA in Cassava under Drought Stress Using cDNA-RAPD Approach Hartati, N. Sri; Supatmi, Supatmi; Aryaningrum, Pramesti Dwi; Sudarmonowati, Enny
Annales Bogorienses Vol. 17 No. 1 (2013): Annales Bogorienses
Publisher : BRIN

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Cassave is an important carbohydrate

Massive In Vitro Propagation of Sandalwood Through Friable Embryogenic Callus Supatmi, Supatmi; Ardiyanti, Nurdiya; Rahman, Nurhamidar; Sudarmonowati, Enny
Annales Bogorienses Vol. 20 No. 1 (2016): Annales Bogorienses
Publisher : BRIN

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Sandalwood (Santalum album), which belongs to Santalaceae family, is a commercially important tree in Indonesia due to its essential oil contents used for important essence of perfume in the perfumery industry. However, its population has significantly depleted since the planting materials of this tree using conventional methods are difficult to be provided. This study was conducted to mass propagate sandalwood using in vitro methods through friable embryogenic callus (FEC). The somatic embryos were formed using leaves in length of 1-3 and 4-7 mm cultured in MS medium containing 0.5 mg/l and 1 mg/l indole acetic acid (IAA), MS medium supplemented with 1 mg/l IAA and 0.2 mg/l kinetin and half concentration of MS medium supplemented with 1 mg/l Gibberellic acid (GA3). Primary somatic embryos (PSE) and secondary somatic embryos (SSE) then formed friable embryogenic callus when it repetitively transferred into MS medium supplemented with 1.7 mg/l BAP, 1 mg/l proline or 1.5 mg/l BAP and 1.2 mg/l kinetin every 3 weeks. The FEC shows its optimum maturation and regeneration in the MS medium supplemented with 1.5 mg/l BAP and 1.2 mg/l kinetin for 4-8 weeks. The acclimatization of sandalwood plantlets were perfectly conducted in the medium containing soil, sand and compos in ratio of 1:1:1 with the companion plant namely Murraya paniculata, (L) Jack which gave the best percentage of survival rate and the lowest percentage of fallen leaves. These findings may improve the massive propagation of sandalwood through FEC as well as a useful material for further genetic improvement of sandalwood by using FEC as material for genetic transformation.

Co-Authors Agus Purwito Ardiyanti, Nurdiya Ardiyanti, Nurdiya Aryaningrum, Pramesti Dwi Aryaningrum, Pramesti Dwi Didy Soepandi, Didy Didy Sopandie Fauzy Rachman Hani Fitriani, Hani Hartati Hartati Hartati, H. Hartati, N Sri I. Fitryatmi Kurnia Sofyan laela Sari, laela N. Sri Hartati, N. Sri N. Sri i Hartati Nurul Khumaida Partomuan Simanjuntak RAGAPADMI PURNAMANINGSIH Rahman, Nurhamidar Rahman, Nurhamidar S. Sadjad Sintho Wahyuning Ardie Siti Kurniawati Sri Hartati Sri N. Hartati, Sri N. Suharsono S, Suharsono Supatmi Supatmi

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