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Uji Aktivitas Antibakteri Ekstrak Daun Jambu Biji (Psidium guajava L.) terhadap Pertumbuhan Bakteri Escherichia coli Niken Niken; Rahmi Novita Yusuf; Annita Annita
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 2 (2022): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i2.5919

Diarrhea is a disease that causes sufferers to have frequent bowel movements with watery or watery stools. One of the causes of diarrhea is Escherichia coli bacteria. One of the plants that has been used as an antidiarrheal is guava (Psidium guajava L.), especially the leaves. Guava leaves are known to contain phytochemicals, namely: tannins, flavonoids, alkaloids, saponins, and essential oils which function as antibacterials. This study aims to determine whether guava leaf extract can inhibit the growth of Escherichia coli bacteria. This research is a laboratory experimental study using the paper disc diffusion method (Kirby-Bauer). The sample used was Escherichia coli bacteria obtained from stool samples of diarrhea patients aged 8-12 years at the Nanggalo Health Center, Padang City, West Sumatra Province. The concentrations used in this study were 10%, 15%, 20%, and positive control for amoxicillin and negative control for distilled water. The results showed that the phytochemical test results for flavonoids, tannins and saponins, and alkaloids were positive. The antibacterial activity of guava leaf extract was shown to have inhibition against Escherichia coli bacteria with an average concentration of 10% with a diameter of 10.7 mm (strong), 15% with a diameter of 13.3 mm (strong), and 20% with a diameter of 17 mm (strong). ), 13 mm amoxicillin positive control, and 0 mm negative control. The results of the ANOVA test showed a value of p = 0.000 (sig. <0.05) that there was a significant difference between all treatments with a positive control. It can be concluded that guava leaf extract effectively inhibits the growth of Escherichia coli bacteria, this is because the diameter of the extract inhibition zone is larger than the positive control.

Exploring the Effects of Bone Marrow Mesenchymal Stem Cells on Amyloid Plaque Reduction in a Rat Model of Alzheimer's Disease Annita Annita; Gusti Revilla; Hirowati Ali; Almurdi Almurdi
Majalah Kedokteran Andalas Vol 46, No 6 (2023): Online Oktober
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i6.p1036-1044.2023

Objective: This study aimed to evaluate the therapeutic potential of Bone Marrow-derived Mesenchymal Stem Cells (BM-MSCs) in mitigating amyloid plaque formation in Alzheimer's disease induced by Aluminum Chloride (AlCl3) in rats. Methods: Rats were divided into three groups: negative control, positive control (AlCl3-induced), and treatment (AlCl3 + BM-MSCs). Cognitive function was assessed five days post-induction, and amyloid plaques were quantified using Congo Red staining in the cortex and hippocampus. Results: A significant reduction in amyloid plaque was observed in rats treated with BM-MSCs. The proportion of stained areas decreased from 1.88 to 1.73 in the cortex and from 1.61 to 1.47 in the hippocampus, compared to AlCl3-only controls. Conclusion: BM-MSCs demonstrated a moderate but statistically significant reduction in amyloid plaque in a rat model of Alzheimer's disease, indicating their therapeutic potential.

ANALISIS PERBANDINGAN MEDIA TERHADAP JUMLAH SEL ADIPOSE MESENCHYMAL STEM CELL Annita Annita; Inelvi Yulia; Mareta Haryani
Jurnal Kesehatan Saintika Meditory Vol 7, No 1 (2024): Mei 2024
Publisher : STIKES Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jsm.v7i1.2806

Sel punca mesenkim adiposa (AD-MSCs) memiliki potensi besar dalam terapi regeneratif. Pemilihan media kultur yang tepat dapat mempengaruhi pertumbuhan dan proliferasi sel. Penelitian ini bertujuan untuk membandingkan efek media Dulbecco's Modified Eagle Medium (DMEM) dan α-Minimum Essential Medium (α-MEM) terhadap pertumbuhan AD-MSCs. AD-MSCs dikultur dalam kedua media dan jumlah sel dihitung menggunakan kamar hitung Neubauer. Hasil penelitian menunjukkan bahwa AD-MSCs yang dikultur dalam α-MEM (178.333 sel/µl) mengalami proliferasi yang secara signifikan lebih tinggi (p=0.013) dibandingkan dengan yang dikultur dalam DMEM (152.333 sel/µl). Penggunaan α-MEM dapat meningkatkan jumlah AD-MSCs yang tersedia untuk aplikasi terapi seluler, sehingga memiliki implikasi penting dalam pengembangan terapi regeneratif.Kata kunci : Sel punca mesenkimal; media kultur; proliferasi sel; hemositometer Neubauer

Adipose-Derived Mesenchymal Stem Cell (AD-MSC)-Like Cells Restore Nestin Expression and Reduce Amyloid Plaques in Aluminum Chloride (AlCl3)-Driven Alzheimer's Rat Models Annita, Annita; Revilla, Gusti; Ali, Hirowati; Almurdi, Almurdi
Molecular and Cellular Biomedical Sciences Vol 8, No 3 (2024)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v8i3.387

Background: Alzheimer's disease (AD) is a neurodegenerative disorder with a significant burden on public health, and current treatments offer limited efficacy. This study investigated the effectiveness of adipose-derived mesenchymal stem cells (AD-MSCs) on the expression of the nestin gene and amyloid plaque in an aluminum chloride (AlCl3)-driven Alzheimer's rat model.Materials and methods: AD-MSCs were characterized using flow cytometry. Adult male Wistar rats were treated with/without AlCl3 and injected with/without AD-MSCs. After 5 days of AlCl3 ingestion and 4 weeks of subsequent AD-MSCs intraperitoneal injection, behavioral and molecular assessments were conducted. The Y-maze alternation test was used to test spatial learning of rats. Nestin gene expression was evaluated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The presence of amyloid plaque in the cortex and the hippocampus was evaluated through Congo red staining.Results: AD-MSC-like cells expressed the MSC markers CD90, CD73 and CD105. The Y-maze alternation result for rats treated with AlCl3 and AD-MSC-like cells was significantly higher compared with rats treated with AlCl3 only. Nestin gene expression was significantly higher in rats treated with AlCl3 and AD-MSC-like cells compared to those treated with AlCl3 only. After AD-MSC-like cells treatment, the Congo red staining results of rat’s cortex and hippocampus were significantly decreased.Conclusion: The findings suggest that AD-MSC-like cells possess therapeutic potential in restoring neural plasticity, amyloid plaque clearance and warrant further investigation for AD treatment. This study contributes to the emerging field of stem cell therapy for neurodegenerative diseases by highlighting the promise of AD-MSCs.Keywords: Alzheimer's disease, adipose-derived mesenchymal stem cells, neural plasticity, congo red staining, stem cell therapy

Exploring the Effects of Bone Marrow Mesenchymal Stem Cells on Amyloid Plaque Reduction in a Rat Model of Alzheimer's Disease Annita, Annita; Revilla, Gusti; Ali, Hirowati; Almurdi, Almurdi
Majalah Kedokteran Andalas Vol. 47 No. 1 (2024): MKA Januari 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v47.i1.p79-86.2024

Objective: This study aimed to evaluate the therapeutic potential of Bone Marrow-derived Mesenchymal Stem Cells (BM-MSCs) in mitigating amyloid plaque formation in Alzheimer's disease induced by Aluminum Chloride (AlCl3) in rats. Methods: Rats were divided into three groups: negative control, positive control (AlCl3-induced), and treatment (AlCl3 + BM-MSCs). Cognitive function was assessed five days post-induction, and amyloid plaques were quantified using Congo Red staining in the cortex and hippocampus. Results: A significant reduction in amyloid plaque was observed in rats treated with BM-MSCs. The proportion of stained areas decreased from 1.88 to 1.73 in the cortex and from 1.61 to 1.47 in the hippocampus, compared to AlCl3-only controls. Conclusion: BM-MSCs demonstrated a moderate but statistically significant reduction in amyloid plaque in a rat model of Alzheimer's disease, indicating their therapeutic potential.

Pengelolaan Sampah Organik Kulit Jeruk Manis menjadi Sabun Mandi Cair: Solusi Ramah Lingkungan dan Pemberdayaan Ekonomi Ibu Rumah Tangga Yulia, Inelvi; Annita, Annita; Haq, Ruqaya Annisa Nurul
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 8, No 12 (2025): Volume 8 No 12 (2025)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v8i12.22646

ABSTRAK Kulit jeruk manis merupakan salah satu limbah yang belum dimanfaatkan dan dianggap tidak bernilai. Padahal kulit jeruk manis mengandung senyawa bioaktif dan aroma yang baik berpotensi untuk dijadikan bahan dasar pembuatan sabun. Pemanfaatan limbah ini tidak hanya berpotensi mendukung upaya pengolahan sampah ramah lingkungan, tetapi juga berpeluang mensejahterakan ekonomi masyarakat. Tujuan kegiatan pengabdian ini adalah untuk memberikan pelatihan pembuatan sabun mandi cair dengan penambahan ekstrak kulit jeruk manis menjadi produk ramah lingkungan serta mendorong pemberdayaan ekonomi ibu rumah tangga di kelurahan Limau Manis, Kota Padang. Metode yang digunakan. Metode yang digunakan berupa pendekatan partisipatif melalui sosialisasi, pelatihan teknis, pendampingan pembuatan sabun cair dari kulit jeruk manis, serta pemantauan perkembagan masyarakat setelah pelatihan. Hasil kegiatan menunjukkan ibu rumah tangga mengalami peningkatan pengertahuan tentang pengolahan limbah kulit jeruk manis serta keterampilan dalam memproduksi sabun mandi cair. Produk yang dihasilkan memiliki aroma khas jeruk, busa yang cukup stabil, dan diterima baik oleh peserta. Selain itu, mitra termotivasi untuk mengembangkan usaha kecil berbasis produk sabun alami, sehingga membuka peluang ekonomi tambahan. Pengolahan limbah kulit jeruk manis menjadi sabun cair terbukti memberikan manfaat ganda, yaitu sebagai solusi ramah lingkungan dalam mengurangi sampah organik dan sebagai sarana pemberdayaan ekonomi ibu rumah tangga melalui keterampilan wirausaha baru. Kata Kunci: Limbah organik, Kulit Jeruk Manis, Sabun Mandi Cair, Ibu Rumah Tangga. ABSTRACT Sweet orange peel is a waste product that is underutilized and considered worthless. However, sweet orange peel contains bioactive compounds and a pleasant aroma, making it a potential raw material for soap production. Utilizing this waste not only supports environmentally friendly waste management efforts but also offers the potential to improve the community's economic well-being. The purpose of this community service activity is to provide training in making liquid soap by adding sweet orange peel extract to create an environmentally friendly product and to encourage the economic empowerment of housewives in Limau Manis sub-district, Padang City. The method used is a participatory approach through outreach, technical training, mentoring in making liquid soap from sweet orange peel, and monitoring community development after the training. The results of the activity show that housewives experienced an increase in knowledge about processing sweet orange peel waste and skills in producing liquid soap. The resulting product has a distinctive citrus aroma, a fairly stable foam, and is well received by participants. In addition, partners are motivated to develop small businesses based on natural soap products, thereby opening up additional economic opportunities. Processing sweet orange peel waste into liquid soap has been proven to provide dual benefits, namely as an environmentally friendly solution in reducing organic waste and as a means of economic empowerment for housewives through new entrepreneurial skills. Keywords: Organic Waste, Sweet Orange Peel, Liquid Bath Soap, Housewives

Roselle (Hibiscus sabdariffa L.) Tea Protects Against Alcohol-Induced Renal Toxicity by Enhancing Endogenous Antioxidant Defence in Wistar Rats Putri Dafriani; Eliza Arman; Annita Annita
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 27 No. 03 (2026): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464) In Progress
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol27-iss03/688

In Indonesia, the choice between area source and smoothed seismicity approaches has been driven by data availability rather than scientific evaluation, and no systematic comparison of both methods at the national scale has been conducted. This study compared both approaches across Indonesia to evaluate their differences in PGA estimation and assess the forecasting skill of the smoothed seismicity model. Both models were applied to the same declustered earthquake catalog with a Magnitude of Completeness of 4.7, b-value of 0.89, and a-value of 8.753, using identical Ground Motion Prediction Equations within a probabilistic seismic hazard analysis framework. Both models consistently identified North Sulawesi as the highest hazard region and Kalimantan as the lowest. The smoothed seismicity model produced higher maximum PGA values of 2.13 g compared to 1.1597 g and 1.5901 g from the area source model at 10% and 2% probability of exceedance in 50 years, respectively. Molchan Diagram validation yielded an Area Skill Score of 0.88 and R-score of 0.656, confirming strong forecasting skill. The two methods are complementary, and their integration within a logic tree framework is recommended for future national-scale PSHA in Indonesia.

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