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Hirowati Ali, Hirowati
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Vitamin D levels in nasopharyngeal carcinoma of the Minangkabau ethnic group Rahman, Sukri; Ali, Hirowati; Indra, Beni
Oto Rhino Laryngologica Indonesiana Vol. 53 No. 1 (2023): VOLUME 53, NO. 1 JANUARY - JUNE 2023
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32637/orli.v53i1.613

Abstract

Background: The etiology of nasopharyngeal carcinoma (NPC) is not yet known with certainty, but is suspected to be an interaction of multiple factors. Nasopharyngeal carcinoma is closely related to Epstein-Barr virus (EBV) infection which interacts with genetic susceptibility and environmental factors. Various studies have reported the relationship of vitamin D with the risk of various cancers, but very little research has a relationship with NPC. Objective: This study was conducted to analyze the association of vitamin D levels with nasopharyngeal carcinoma in the Minangkabau ethnic group. Methods: This study was a cross-sectional study comparing plasma levels of vitamin D (25(OH)D) in new patients with NPC of the Minangkabau ethnic who were treated at Dr. M. Djamil Padang with healthy control plasma. Result: This study found that the average vitamin D level in the NPC group was higher than the control group. Vitamin D levels in this study varied in both cases and controls. In the NPC group, vitamin D levels varied from 14.64 to 75.56 ng/ml. Most of the NPC group had normal vitamin D levels (69.6%), and in controls, vitamin D levels also varied from 6.06 to 73. .96 ng/ml, and most of them were also with normal levels (52.2%). There was no statistically significant difference between cases and controls (p>0.05). Conclusion: This study found no association between the level of vitamin D and the incidence of nasopharyngeal carcinoma in the Minangkabau ethnic group.
Analysis of Factors Relating to Pregnant Women's Knowledge About Triple Elimination at Andalas Health Center, Padang City Aisyah, Siti; Basyir, Vaulinne ,; Wydya, Satya; Sriyanti, Roza; Silviam, Nelmi; Ali, Hirowati
Madani: Jurnal Ilmiah Multidisiplin Vol 2, No 3 (2024): Madani, Vol. 2, No. 3 2024
Publisher : Penerbit Yayasan Daarul Huda Kruengmane

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.11093691

Abstract

Pregnant mother is group woman vulnerable at risk transmit Human Immunodeficiency Virus (HIV), Hepatitis B, and Syphilis to fetus . Effort disconnect transmission of HIV, Syphilis , and Hepatitis B from Mother pregnant to the fetus is one of them with inspection triple elimination . Objective study For know related factors with knowledge Mother pregnant about triple elimination at the Community Health Center Andalas. Study This is study quantitative with analytical and observation methods design cross sectional carried out in the Puskesmas area Andalas . Sample study totaling 98 mothers pregnant taken with method consecutive sampling. Primary data collection uses questionnaire and analyzed with chi square and regression tests logistics. Research result univariate part big own low education , no work , supportive husband , support power poor health , and exposure insufficient information . Analysis bivariate using the chi square test there is significant relationship between education ( p-value 0.011), employment ( p-value 0.038), support power health ( p-value 0.020), exposure information ( p-value 0.039) with knowledge Mother pregnant about triple elimination . No there is connection support husband ( p-value 0.453) with knowledge Mother pregnant about triple elimination . Analysis Multivariate using regression testing logistics obtained related factors with knowledge Mother pregnant about triple elimination is exposure information and education , the most dominant factors is education ( p-value 0.009). Conclusions of educational Research identified as factor related dominant to knowledge Mother pregnant about triple elimination. Expected Public health centers in Andalas do cooperate cross sector to improve knowledge Mother about triple elimination as an effort promotional and preventive in prevent HIV, Syphilis, and Hepatitis B.
Peran Heparan Sulfate, Chondroitin Sulfate, dan Hyaluronic Acid Sebagai Biomarker Diagnosis Sepsis Neonatus Aswati, Lydia; Yusrawati, Yusrawati; Yani, Finny Fitry; Ali, Hirowati
Sari Pediatri Vol 26, No 4 (2024)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp26.4.2024.257-62

Abstract

Sepsis neonatus menyebabkan morbiditas dan mortalitas yang bermakna. Berdasarkan penelitian pada populasi di  dua dekade terakhir, diperkirakan insiden sepsis neonatus secara global adalah 2202 per 100.000 kelahiran hidup, dengan mortalitas antara 11-19%. Kultur darah masih menjadi gold standar untuk konfirmasi diagnosis sepsis neonatus, tetapi masih terbatas untuk mendapatkan hasilnya, diperlukan waktu sekitar 24-72 jam. Sepsis berhubungan dengan disfungsi sel endotel yang berat. Disfungsi endotel dapat disebabkan oleh oksidan, hiperglikemia, ROS, endotoksin bakteri serta sepsis. Disfungsi endotel pada sepsis neonatus terjadi penumpahan dari bahan-bahan yang menyusun lapisan glikokaliks endotel. Lapisannya  disusun oleh  proteoglikan, glikoprotein dan glikosaminoglikan. Komponen glikokaliks yang berada di  sirkulasi seperti syndecan-1, hyaluronan, heparan sulfate dan chondroitin sulfate dapat ditemukan di plasma/serum dan urin, diharapkan dapat digunakan  sebagai biomarker diagnosis sepsis neonatus.
Differences In Mesenkimal Stem Cell Proliferation Between Bone Marrow Type Stem Cells And Wharton's Jelly Type Stem Cells Mahendika, Devin; Arif, Muhammad; Julita, Ema; Ali, Hirowati
Jurnal EduHealth Vol. 15 No. 02 (2024): Jurnal eduHealt, Edition April - June , 2024
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Various sources of stem cells in the human body, one of which is mesenchymal stem cells derived from bone marrow and Wharton's jelly. This study aims to determine differences in the proliferation count results of 2 stem cells from different sources, bone marrow and Wharton's jelly. The research was conducted using a true experimental design with in-vitro studies. Bone marrow and Wharton's jelly MSC were counted for their proliferation results at 24 hours, 48 hours, and 72 hours. The data then compared using the T-test method. The results of the study showed differences the results of the proliferation count between bone marrow and Wharton's jelly. Qualitatively, Wharton's jelly was seen in 24 hours, 48 hours, or 72 hours. However, quantitatively with statistical tests, the results that were not significant in the first 24 hours (p=0.053). At 48 hours, statistically significant results (p=0.007) were obtained because the cells began to proliferate rapidly and gave a clear difference, and at 72 hours, insignificant statistical results (p 0.074) were found because the cells were confluent and filled the substrate.The ability to reproduce is one of the characteristics possessed by stem cells. This process can be influenced by many factors so that between a culture can vary the speed of proliferation. Proliferation count showed that Wharton's jelly was more than bone marrow in 24 hours, 48 hours, or 72 hours. Different sources of MSC yield different proliferation results.
Pengaruh Ekstrak Etanol Peronema canescens Jack terhadap Ekspresi Gen Tumor Necrosis Factor Alpha pada Sel HeLa Ansharina, Hafiznie; Revilla, Gusti; Kadri, Husnil; Desmawati, Desmawati; Yusri, Elfira; Ali, Hirowati
Health and Medical Journal Vol 7, No 1 (2025): HEME January 2025
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/heme.v7i1.1418

Abstract

Latar Belakang: Kanker serviks merupakan kanker yang menduduki peringkat keempat pada wanita di seluruh dunia. Kanker ini disebabkan oleh Human Papillomavirus (HPV) tipe resiko tinggi yang menyerang sel-sel leher rahim. Sel kanker akan mensekresi Tumor Necrosis Factor Alpha (TNF-α) untuk menjadikan lingkungan mikrotumor mendukung bagi kelangsungan hidup sel kanker. Terapi kanker serviks yang umum digunakan adalah pembedahan, radioterapi, dan kemoterapi. Terapi lain yang juga digunakan adalah tanaman herbal, contohnya sungkai. Ekstrak etanol daun sungkai (Peronema canescens J) mengandung senyawa yang berperan sebagai antiinflamasi dan antioksidan. Tujuan: Penelitian ini bertujuan untuk mengetahui pemberian ekstrak etanol daun sungkai dalam menurunkan ekspresi gen TNF-α pada sel HeLa. Metode: Penelitian ini merupakan penelitian true experimental dengan post test only control group design yang menggunakan 24 well plate kultur sel HeLa yang tumbuh secara konfluens. Sampel dibagi menjadi empat kelompok (K, P1, P2, dan P3). Kelompok perlakuan diberikan ekstrak etanol daun sungkai dengan konsentrasi inhibition concentration (IC)25, IC50, dan IC75 yang diinkubasi selama 24 jam. Sel yang telah diberi perlakuan akan dipanen untuk dilakukan isolasi RNA. Pemeriksaan gen TNF-α dilakukan menggunakan PCR Konvensional, kemudian band elektroforesis berukuran 47 bp dianalisis menggunakan ImageJ. Hasil: Data didapatkan dari analisis ketebalan band elektroforesis yang diukur menggunakan ImageJ. Analisis data dilakukan menggunakan uji One Way ANOVA dan Post Hoc Bonferroni, didapatkan rerata ekspresi gen TNF-α pada kelompok K, P1, P2, dan P3 secara berturut-turut adalah 0,32; 0,29; 0,23; dan 0,14. Terdapat perbedaan yang bermakna antara kelompok K terhadap P2 dan P3 dengan nilai p=0,000 (p<0,05), sedangkan kelompok K dengan P1 perbedaannya tidak bermakna (p>0,05). Kesimpulan: Pemberian ekstrak etanol daun sungkai pada konsentrasi IC50 dan IC75 mampu menurunkan ekspresi gen TNF-α pada kelompok sel HeLa.
Adipose-Derived Mesenchymal Stem Cell (AD-MSC)-Like Cells Restore Nestin Expression and Reduce Amyloid Plaques in Aluminum Chloride (AlCl3)-Driven Alzheimer's Rat Models Annita, Annita; Revilla, Gusti; Ali, Hirowati; Almurdi, Almurdi
Molecular and Cellular Biomedical Sciences Vol 8, No 3 (2024)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v8i3.387

Abstract

Background: Alzheimer's disease (AD) is a neurodegenerative disorder with a significant burden on public health, and current treatments offer limited efficacy. This study investigated the effectiveness of adipose-derived mesenchymal stem cells (AD-MSCs) on the expression of the nestin gene and amyloid plaque in an aluminum chloride (AlCl3)-driven Alzheimer's rat model.Materials and methods: AD-MSCs were characterized using flow cytometry. Adult male Wistar rats were treated with/without AlCl3 and injected with/without AD-MSCs. After 5 days of AlCl3 ingestion and 4 weeks of subsequent AD-MSCs intraperitoneal injection, behavioral and molecular assessments were conducted. The Y-maze alternation test was used to test spatial learning of rats. Nestin gene expression was evaluated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The presence of amyloid plaque in the cortex and the hippocampus was evaluated through Congo red staining.Results: AD-MSC-like cells expressed the MSC markers CD90, CD73 and CD105. The Y-maze alternation result for rats treated with AlCl3 and AD-MSC-like cells was significantly higher compared with rats treated with AlCl3 only. Nestin gene expression was significantly higher in rats treated with AlCl3 and AD-MSC-like cells compared to those treated with AlCl3 only. After AD-MSC-like cells treatment, the Congo red staining results of rat’s cortex and hippocampus were significantly decreased.Conclusion: The findings suggest that AD-MSC-like cells possess therapeutic potential in restoring neural plasticity, amyloid plaque clearance and warrant further investigation for AD treatment. This study contributes to the emerging field of stem cell therapy for neurodegenerative diseases by highlighting the promise of AD-MSCs.Keywords: Alzheimer's disease, adipose-derived mesenchymal stem cells, neural plasticity, congo red staining, stem cell therapy
Peran Ki67 pada Karsinoma Nasofaring Hakikah, Tika; Rahman, Sukri; Ali, Hirowati
Jurnal Otorinolaringologi Kepala dan Leher Indonesia Vol. 3 No. 1 (2024)
Publisher : Fakultas Kedokteran Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jokli.v3i1.64

Abstract

Latar Belakang: Pendahuluan: Karsinoma nasofaring memiliki rekurensi atau metastasis jauh, yang menandakan suatu adanya aktifitas sel yaitu proliferasi sel. Penilaian adanya suatu proliferasi sel memerlukan biomarker, salah satunya Ki67 yang sampai saat ini sudah dikenal baik sebagai penanda proliferasi sel. Tujuan: Mengetahui peran Ki67 pada karsinoma nasofaring. Tinjauan Pustaka: Karsinoma nasofaring adalah tumor ganas sel skuamosa yang terjadi di daerah perbatasan epitel nasofaring dimana epitel kubus beralih menjadi epitel skuamosa. Pada stadium dini tidak mudah dideteksi karena secara anatomis terletak jauh di dalam kepala. Ki67 berhubungan erat dengan mitosis dan aktivitas proliferasi tumor ganas serta kinerja pengobatan kanker nasofaring. Kesimpulan: Ekspresi Ki67 yang tinggi merupakan penanda untuk prognosis buruk pada karsinoma nasofaring. ABSTRACT Background: Introduction: Nasopharyngeal carcinoma has recurrence or distant metastasis, which indicates a cell activity, namely cell proliferation. Assessment of the presence of a cell proliferation requires biomarkers, one of which is Ki67 which has been established as a marker of cell proliferation. Objective: To determine the role of Ki67 in nasopharyngeal carcinoma. Literature Review: Nasopharyngeal carcinoma is a malignant squamous cell tumor that occurs in the border area of the nasopharyngeal epithelium where the cuboidal epithelium turns into the squamous epithelium. At an early stage, it is difficult to detect because it is anatomically located deep in the head. Ki67 is closely related to the mitotic and proliferative activity of malignant tumors and the treatment performance of nasopharyngeal carcinoma. Conclusion: High Ki67 expression is a marker for poor prognosis in nasopharyngeal carcinoma.    
Gambaran Kualitas Hidup Santriwati yang Menderita Skabies di Pondok Pesantren Kecamatan Enam Lingkung, Kabupaten Padang Pariaman Febrina, Widia; Harminarti, Nora; Ali, Hirowati
Jurnal Kesehatan Andalas Vol. 9 No. 4 (2020): Online December 2020
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v9i4.1504

Abstract

Kualitas hidup terkait dengan kesehatan  meliputi aspek fisik, psikis dan sosial. Penilaian kualitas hidup tersebut terdiri dari: tidak ada pengaruh, pengaruh kecil, pengaruh sedang dan pengaruh besar terhadap kualitas hidup karena penyakit skabies. Tujuan: Mengetahui gambaran kualitas hidup santriwati yang menderita skabies di Pondok Pesantren Kecamatan Enam Lingkung, Kabupaten Padang Pariaman. Metode: Jenis penelitian ini adalah deskriptif dengan desain cross sectional. Pondok Pesantren Kecamatan Enam Lingkung Kabupaten Padang Pariaman pada bulan Desember 2019. Pengambilan sampel dilakukan dengan teknik total sampling dengan jumlah sampel sebanyak 32 orang. Data dikumpulkan melalui wawancara, pemeriksaan kulit dan pengerokan kulit. Proses wawancara yang dipandu kuesioner modified Dermatology Life Quality Index yang sudah diterjemahkan dari Bahasa Inggris ke Bahasa Indonesia.  Hasil: Mayoritas penyakit skabies berpengaruh kecil terhadap kualitas hidup responden (62,5%), diikuti dengan pengaruh sedang pada kualitas hidup (25,0%), selanjutnya tidak ada pengaruh pada kualitas hidup (4%) dan pengaruh besar pada kualitas hidup (0%). Komponen kualitas hidup yang paling terganggu adalah kegiatan sekolah/belajar (18,8%) dan yang paling tidak terpengaruh adalah hubungan pertemanan (78,1%). Simpulan: Mayoritas penyakit skabies memiliki pengaruh yang kecil terhadap kualitas hidup penderita skabies.Kata kunci: modified dermatology life quality index, kualitas hidup, skabies
Pengaruh Pemberian Mesenchymal Stem Cells Wharton's Jelly terhadap Ekspresi Gen Sirtuin 1 pada Tikus Model Alzheimer Aizah, Havina Nurul; Yanis, Amel; Ali, Hirowati; Tofrizal, Tofrizal; Ilmiawati, Cimi; Arisanty, Dessy
Jurnal Kesehatan Andalas Vol. 12 No. 1 (2023): Online March 2023
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v12i1.2140

Abstract

Mesenchymal Stem Cells Wharton's Jelly (MSC-WJ) has become a new hope for Alzheimer's disease due to its capability to suppress Amyloid-beta (Aβ) plaques formation. It is found that people with Alzheimer's disease have a lower expression of Sirtuin-1 because of the oxidative stress that was produced by Aβ plaques. Objective: To observe the Sirtuin-1 gene in Alzheimer's disease rat models that AlCl3 was inducing after being given MSC-WJ. Methods: This was an experimental study with a post-test-only control group design, including three groups with a total sample of 18 samples of Alzheimer RNA rat models. Group-1without AlCl3 and MSC-WJ; Group-2 with AlCl3; Group-3 AlCl3 and MSC-WJ. Mean value of the Sirtuin-1 gene was obtained by the Image-J application using a semiquantitative method which was then tested using the One Way ANOVA test. The study was significant if the p-value < 0,05. Results: This study found that the mean ± standard error value of the Sirtuin-1 gene in three groups were 0.18±0.04; 0.16±0.03; and 0.36±0.06 respectively. The statistical test showed a significant difference between the three groups (p = 0.014). Conclusion: There was an increased Sirtuin 1 gene expression in Alzheimer’s rat Model after being given Mesenchymal Stem Cells Wharton’s Jelly.Keywords:  Alzheimer, MSC-WJ, Sirtuin-1
Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer Putri, Nabila Priscilla; Ali, Hirowati; Tofrizal, Tofrizal; Darwin, Eryati; Susanti, Restu; Hasmiwati, Hasmiwati
Jurnal Kesehatan Andalas Vol. 12 No. 2 (2023): Online July 2023
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v12i2.2142

Abstract

The increased production of beta-amyloid marks Alzheimer's disease. PPAR-γ shows involvement in Alzheimer's disease. PPAR-γ Gene is suspected to reduce beta-amyloid plaques and reduce inflammation and oxidative stress in Alzheimer's disease. Mesenchymal Stem Cells Wharton's Jelly (MSC-WJ) is expected to increase the PPAR-γ Gene expression. Objective:  To observed the PPAR-γ Gene expression in Alzheimer's Mice. Methods: The mice are induced using AlCl3 and were given MSC-WJ. This study was an experimental study with a post-test-only control group design on 18 samples of RNA from Alzheimer's mice that were divided into 3 groups. Negative control group (K-), positive control group (K+), and experimented group (P). The mean of PPAR-γ gene expression was obtained by comparing the PPAR-γ gene with the GAPDH gene. This study used semiquantitative methods using ImageJ. Data analysis was used with the Kruskal-Wallis test. It is significant if the value is <0,05. Results: The mean ratio of PPAR-γ gene expression that was obtained in K-, K+, and P were 0.12, 0.06, and 0.08, respectively. There were significant differences between each group, with a p-value of 0,023 (p<0,05). Conclusion: Giving MSC-WJ could increase the PPAR-γ gene expression. Further study using real-time PCR is highly needed to increase the MSC-WJ treatment in patients with Alzheimer's disease.Keywords:  Alzheimer disease, MSC-WJ, PPAR- γ
Co-Authors Afriwardi Afriwardi Aizah, Havina Nurul Almurdi Almurdi Amel Yanis Anggraini, Debie Annita, Annita Ansharina, Hafiznie Atmi, Serly Rahma Basyir, Vaulinne , Beni Indra, Beni Cimi Ilmiawati, Cimi Desmawati Desmawati Desmawati Desmawati Dessy Arisanty Dian Pertiwi Dina Arfiani Rusjdi Djong Hon Tjong Eryati Darwin Fadila, Zurayya Fathiyyatul Khaira, Fathiyyatul Febrina, Widia Ferdinand Ferdinand, Ferdinand Finny Fitry Yani Gestina Aliska Gusti Revilla Hakikah, Tika Hasmiwati Husnil Kadri Julita, Ema Kencana, Gita Lydia Aswati, Lydia Mahata, Liganda Endo Mahendika, Devin Mazara, Fitria Muhammad Arif Muthara, Indah Nora Harminarti Noverial, Noverial Nur'aisyah, Shafira Perdanakusuma, David Sontani Putri, Biomechy Oktomalio Putri, Nabila Priscilla Rahmadian, Rizki Rahmatini . Rasya, Salsabila Faiha Wiendra Restu Susanti Sahputra, Roni Eka Saputri, Rahmi Agu Silviam, Nelmi Siregar, Muhammad Abi Ghoffari Siti Aisyah Sriyanti, Roza Sukri Rahman Tofrizal Wydya, Satya Yarni, Sisca Dwi Yusrawati Yusrawati Yusri, Elfira Zelly Dia Rofinda