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All Journal HAYATI Journal of Biosciences TEKNIK JURNAL SAINS DAN TEKNOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Makara Journal of Science Makara Journal of Technology METRIK SERIAL TEKNOLOGI DAN SAINS Annales Bogorienses Berita Biologi
Rofiq Sunaryanto
Balai Pengkajian Bioteknologi, BPPT, Kawasan Puspiptek Serpong, Tangerang Selatan 15314, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Other

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PENENTUAN KOMBINASI MEDIUM TERBAIK GALAKTOSA DAN SUMBER NITROGEN PADA PROSES PRODUKSI ETANOL Sunaryanto, Rofiq; Handayani, Berti Hariasih
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 1 (2015): June 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (800.98 KB) | DOI: 10.29122/jbbi.v2i1.531

Abstract

Ethanol is an important product for biotechnology-based industries. Ethanol can be produced from various raw materials and some types of microbes. Determination of the best combination of galactose with nitrogen sources on ethanol production using Saccharomyces cerevisiae has been done. Combination of galactose at the concentration of 3 g/L and 20 g/L with nitrogen sources (casein, peptone, and urea, each at the concentration of 10g/L) was used to obtain the best composition of fermentation medium. Fermentation was carried out for 60 hours at 30°C, 250 rpm, and working volume of 50 mL in a 250 mL erlenmeyer. The results showed that the galactose concentration of 20 g/L was able to improve the productivity of ethanol and the growth of S. cerevisiae cells. The combination of 20g/L galactose and 10 g/L casein produced the highest ethanol concentration (6% v/v), whereas 20 g/L galactose-10 g/L peptone and 20 g/L galactose-10 g/L urea combinations produced 2.5% and 0.58% (v/v) ethanol, respectively. The use of 3 g/L galactose mixed with several nitrogen sources produced ethanol below 0.7% (v/v). Keywords: Ethanol, galactose, peptone, casein, Saccharomyces cerevisiaeABSTRAKEtanol merupakan salah satu produk penting bagi industri yang berbasis bioteknologi. Etanol dapat dihasilkan dari berbagai macam bahan baku dan beberapa jenis mikroba. Penentuan kombinasi terbaik antara galaktosa dengan sumber nitrogen pada produksi etanol menggunakan Saccharomyces cerevisiae telah dilakukan. Konsentrasi galaktosa 3 g/L dan galaktosa 20 g/L yang dikombinasikan dengan sumber nitrogen dengan konsentrasi 10 g/L dalam hal ini kasein, pepton, dan urea digunakan sebagai perlakuan untuk mendapatkan kombinasi medium sumber karbon dan sumber nitrogen terbaik. Fermentasi untuk menghasilkan etanol dilakukan selama 60 jam pada suhu 30°C, agitasi 250 rpm dengan volume kerja 50 mL dalam erlenmeyer 250 mL. Hasil penelitian menunjukkan penambahan galaktosa dengan konsentrasi sampai dengan 20 g/L mampu memperbaiki produktivitas etanol dan pertumbuhan sel S. cerevisiae. Konsentrasi 20 g/L galaktosa dengan 10 g/L kasein menghasilkan produktivitas etanol paling tinggi yaitu 6%(v/v), konsentrasi galaktosa 20 g/L dengan 10 g/L pepton menghasilkan 2,5% (v/v) etanol dan konsentrasi galaktosa 20 g/L dengan 10 g/L urea menghasilkan 0,58%(v/v) etanol. Penggunaan konsentrasi galaktosa 3 g/L yang dikombinasikan dengan beberapa jenis sumber nitrogen menghasilkan etanol dibawah 0,7% (v/v).Kata kunci: Etanol, galaktosa, pepton, kasein, Saccharomyces cerevisiae
PENGARUH KOMBINASI BAKTERI ASAM LAKTAT TERHADAP PERUBAHAN KARAKTERISTIK NUTRISI SUSU KERBAU Sunaryanto, Rofiq
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 4 No. 1 (2017): June 2017
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.366 KB) | DOI: 10.29122/jbbi.v4i1.2064

Abstract

Effects of Lactic Acid Bacteria Combination on Characteristic Change of Buffalo Milk Nutrition Determination of physical and chemical characteristics on fermented milk using a mixture of Lactobacillus bulgaricus, Streptococcus thermophilus, and Bifidobacterium sp. has been conducted. Fermentation was carried out for 20 hours at 37ºC in a facultative aerobic condition. Physical characteristics were performed by comparing the physical properties before and after fermentation such as viscosity and texture, including chemical properties such as pH, acidity, protein, fat, and sugar contents. Inoculants of lactic acid bacteria were varied using a combination of lactic acid bacteria mixture. Results showed that the combination of bacteria inoculants used has no significant effect on changes in protein, fat, ash, and water contents. However, the effects on total acid, pH, sugar content, and physical appearance such as viscosity and texture were significant. The combination of L. bulgaricus, S. thermophilus and Bifidobacterium sp. (A6) produced a soft texture appearance and high viscosity. The combination of inoculant bacteria L. bulgaricus with S. thermophilus (A5) produced a texture similar to A6 but with a lower viscosity than A6.Keywords: Lactobacillus bulgaricus, Streptococcus thermophilus, Bifidobacterium sp, buffalo milk, fermentation. ABSTRAKPenelitian untuk mengetahui karakteristik fisik dan kimia hasil fermentasi susu kerbau menggunakan campuran Lactobacillus bulgaricus, Streptococcus thermophilus, dan Bifidobacterium sp telah dilakukan. Fermentasi dilakukan selama 20 jam pada suhu 37ºC secara aerobe fakultatif. Karakteristik fisika dilakukan dengan membandingkan sifat fisik seperti kekentalan dan tekstur, serta sifat kimia yang meliputi kandungan protein, lemak, gula, pH, keasaman sebelum dan sesudah fermentasi. Inokulan bakteri asam laktat divariasikan menggunakan kombinasi campuran bakteri asam laktat. Hasil penelitian menunjukkan bahwa kombinasi inokulan yang digunakan dalam proses fermentasi susu kerbau tidak berpengaruh nyata terhadap perubahan kadar protein, lemak, abu, dan air. Namun demikian berpengaruh nyata terhadap total asam, pH, kadar gula, dan penampakan fisik seperti kekentalan dan tekstur. Kombinasi inokulan L. bulgaricus, S. thermophilus dan Bifidobacterium sp. (A6) menghasilkan susu fermentasi dengan penampakan tekstur halus dan kekentalan yang lebih padat. Kombinasi bakteri L. bulgaricus dengan  S. thermophilus (A5) menghasilkan tekstur yang mirip dengan A6, namun demikian menghasilkan kekentalan yang lebih rendah dibandingkan A6.Kata kunci: Lactobacillus bulgaricus, Streptococcus thermophilus, Bifidobacterium sp, susu  kerbau, fermentasi
OPTIMALISASI MEDIA PRODUKSI AMILOGLUKOSIDASE MENGGUNAKAN FERMENTASI MEDIA PADAT Sunaryanto, Rofiq; Marasabessy, Ahmad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 1 (2016): June 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.689 KB) | DOI: 10.29122/jbbi.v3i1.19

Abstract

Amiloglukosidase merupakan salah satu enzim yang banyak digunakan dalam industri gula cair. Dalam proses fermentasi, komposisi medium fermentasi sangat berpengaruh terhadap aktivitas amiloglukosidase. Optimalisasi media padat proses produksi amiloglukosidase secara fermentasi padat dengan menggunakan isolat Aspergillus niger telah dilakukan. Optimalisasi media padat dilakukan dengan menentukan rasio dedak:pati terbaik yang selanjutnya dikombinasikan dengan beberapa sumber nitrogen. Dalam penelitian ini sumber nitrogen yang digunakan adalah sumber nitogen organik dan sumber nitrogen anorganik. Sumber nitrogen organik yang digunakan antara lain Corn Step Liquor dan pepton, adapun sumber nitrogen anorganik yang digunakan adalah amonium nitrat, amonium sulfat, dan amonium pospat. Fermentasi dilakukan selama 120 jam pada suhu 30ºC.Hasil percobaan menunjukkan bahwa kombinasi perbandingan media sumber karbon dedak:pati 1:1 menghasilkan produktivitas amiloglukosidase tertinggi 724 Unit/ml. Amonium sulfat merupakan sumber nitrogen anorganik terbaik dan mampu menghasilkan produktivitas amiloglukosidase sebesar 823 Unit/ml, sedangkan Corn Step Liquor (CSL) yang merupakan sumber nitrogen organik mampu menghasilkan produktivitas amiloglukosidase lebih tinggi dibandingkan pepton dengan produktivitas sebesar 884 Unit/ml.Keywords: Aspergillus niger, amyloglucosidase, fermentation, carbon source, nitrogen source  ABSTRAKAmiloglukosidase adalah salah satu enzim yang banyak digunakan dalam industri gula cair. Dalam proses fermentasi, komposisi medium fermentasi sangat berpengaruh terhadap aktivitas amiloglukosidase. Pada penelitian ini telah dilakukan optimalisasi media padat pada proses produksi amiloglukosidase secara fermentasi padat dengan menggunakan isolat Aspergillus niger. Optimalisasi media padat dilakukan dengan menentukan rasio dedak:pati terbaik yang selanjutnya dikombinasikan dengan beberapa sumber nitrogen. Sumber nitrogen yang digunakan adalah sumber nitrogen organik dan anorganik. Sumber nitrogen organik yang digunakan antara lain adalah Corn Step Liquor (CSL) dan pepton, adapun sumber nitrogen anorganik yang digunakan adalah amonium nitrat, amonium sulfat, dan amonium fosfat. Fermentasi dilakukan selama 120 jam pada suhu 30ºC. Hasil percobaan menunjukkan bahwa kombinasi perbandingan media sumber karbon dedak:pati 1:1 menghasilkan produktivitas amiloglukosidase tertinggi, yaitu 724 unit/mL. Amonium sulfat merupakan sumber nitrogen anorganik terbaik dan mampu menghasilkan produktivitas amiloglukosidase sebesar 823 unit/mL, sedangkan CSL menghasilkan produktivitas amiloglukosidase lebih tinggi dibandingkan pepton, yakni sebesar 884 unit/mL.Kata kunci: Aspergillus niger, amiloglukosidase, fermentasi, sumber karbon, sumber nitrogen
Optimizing Fermentation Medium to Produce Cyclosporin a Using Response Surface Methodology Sunaryanto, Rofiq
Makara Journal of Technology Vol. 16, No. 1
Publisher : UI Scholars Hub

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Abstract

Response surface methodology (RSM) is a three factorial model which illustrates the relationship between one or more independent variables. RSM can be used to optimize the fermentation medium for the production of Cyclosporin A from the isolate Tolypocladium inflatum. The optimal point of the response surface area is predicted by using a seconddegree polynomial model and applying the statistic model obtained from the central composite design (CCD). The results of optimizing the fermentation medium for Cyclopsorin using the three independent variables of glucose, casein, and KH2PO4 show that all three of the independent variables affect the production of Cyclosporin A. There is a positive interaction between the independent variables of glucose and casein, however, there is no visible interaction between glucose with KH2PO4 and casein with KH2PO4. By using the mathematical model the total optimum result obtained is 1230.5 mg L-1, glucose concentrate 28.5 g L-1, KH2PO4 concentrate 0.74 gL-1, and casein concentrate 9.8 g L-1. Laboratory validation shows that Cyclosporin A productivity is 1197.285 mg L-1. There is a value difference of 2.7% between the expected productivity of Cyclosporin A using the mathematical model and the actual production in laboratory tests.
Isolation, Purification, and Characterization of Antimicrobial Substances from Endophytic Actinomycetes Sunaryanto, Rofiq; Mahsunah, Anis Herliyani
Makara Journal of Science Vol. 17, No. 3
Publisher : UI Scholars Hub

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Abstract

Antimicrobial active substances produced by endophytic actinomycetes were isolated and purified. Plant samples were obtained from four different medicinal plants namely Curcuma domestica, Phaleria macrocarpa, Isotoma longiflora, and Symplocos cocchinensis. Isolation of actinomycetes was conducted using HV agar with the addition of cycloheximide, nystatin, nalidixic acid, and rifamycin. A total of 21 actinomycete isolates were obtained and tested for antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, and Bacillus subtilis ATCC 66923. Among the isolates, isolate KY01 was the most active to Gram-positive and Gram-negative bacteria. Morphological observation and identification using 16S rRNA gene sequence showed that the isolate KY01 was similar to Streptomyces antibioticus. An active compound from the isolate KY01 was produced using yeast peptone medium. The active compound was purified using silica-gel-column chromatography and preparative high performance liquid chromatography (HPLC). A single peak of the active compound was detected with HPLC and LCMS, which showed a retention time of 26.6 min and molecular weight (MW) 906.4474 g/mol, respectively.
Pengaruh Penambahan Effective Microorganisms (Em-4) Terhadap Kualitaslimbah Cair Tahu Dengan Teknik Aerasi Sari, Selvi Ayu; Nurhayati, Nurhayati; Sunaryanto, Rofiq
Metrik Serial Teknologi dan Sains Vol. 3 No. 1 (2022)
Publisher : Konsorsium Cendekiawan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51616/teksi.v3i1.276

Abstract

Perkembangan industri dapat meningkatkan pencemaran lingkungan salah satunya adalah Limbah dari Industri Tahu. Industri tahu menghasilkan limbah berupa limbah padat atau limbah cair. Banyaknya industri tahu yang tidak melakukan pengolahan limbah cair yang mengakibatkan terjadinya pencemaran lingkungan di perairan, oleh karena itu diperlukan alternatif dalam pengolahan limbah cair salah satunya menggunakan Effectve Microorganisme (EM-4). Jenis penelitian ini adalah penelitian eksperimen yang bertujuan untuk mengetahui penurunan kadar pencemar pada limbah cair tahu dengan pengolahan limbah menggunakan larutan Effectve Microorganisme (EM-4) menggunakan teknik aerasi dengan variasi waktu tinggal adalah 1 hari, 3 hari, 5 hari, 8 hari. Hasil penelitian menunjukan adanya penurunan kadar pencemar BOD terbesar dengan menggunakan EM4 pada waktu tinggal hari ke 8 dengan nilai BOD 4218,82 mg/L (75,20%), COD 6341,70 mg/L (70,87%), untuk penurunan terbesar parameter Kekeruhan dan TDS terjadi pada hari ke-5 yaitu Kekeruhan sebesar 446,67 NTU (44,5%) dan TDS sebesar 864,67 mg/L (11,23%), dan terjadi kenaikan kadar Kekeruhan dan TDS pada hari ke-8 dengan nilai Kekeruhan sebesar 481 NTU (40,25%), TDS sebesar 865 mg/L (11,19%) kenaikan kadar Kekeruhan dan TDS diduga diakibatkan karena adanya kesalahan pada saat preparasi sampel. Analisis data menggunakan Regresi Linear Sederhana dan dilakukan Uji Hipotesis variansi (Uji F) signifikan α = 0,05 (5%) hasil tersebut menunjukan adanya pengaruh signifikan variasi waktu terhadap penurunan kadar BOD, COD, Kekeruhan, dan TDS.
Marine Actinobacteria amo.128 Isolated from Seribu Island: Antibacterial, Antibiofilm and Molecular Docking as Quorum Sensing Inhibitors Cahyanto, Heru Agus; Sunaryanto, Rofiq; Damayanti, Ema; Mustofa
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1225-1239

Abstract

The prevalence of antibiotic-resistant bacteria is increasing every year in Indonesia. This resistance occurs in several antimicrobial categories. A contributing factor to microbial resistance is the capacity of microbes to develop biofilms. Amo.128 is an actinomycete from the Laboratory Biotechnology, BRIN Serpong collection, which is expected to have both antimicrobial and antibiofilm activity. This study aimed to identify amo.128 macroscopically, microscopically, and molecularly; to determine the antibacterial and antibiofilm activity; to identify secondary metabolites; and to understand the mechanism of quorum sensing inhibition by in silico with proteins targeting SdiA and AgrA. Based on macroscopic and microscopic observations, the amo.128 isolate belongs to the genus Streptomyces. Phylogenetic analysis of the amo.128 isolate is 100% similar to Streptomyces parvus strain NBRC 14599. The amo.128 metabolite contains several compounds, including N-acetyltyramine, cyclophenylalanylprolyl/cFP, and the pyrrole-pyrazine group. The MIC/MBC/MIC50 value of the amo.128 metabolite against Staphylococcus aureus is 25/50/28.48 ppm, while for Escherichia coli it is 100/200/49.38 ppm. The amo.128 metabolite reduced biofilms formed by S. aureus and E. coli with BRC50 values of 62.07 ppm and 60.44 ppm, respectively. The amo.128 metabolite compound, cyclophenylalanylprolyl/cFP, has potential activity as a quorum-sensing inhibitor.
Isolation, Identification, and Evaluation of Antimicrobial Activity of Active Compound Produced by Marine Actinomycetes isolate A32 Sunaryanto, Rofiq; Marwanta, Edy
Annales Bogorienses Vol. 19 No. 2 (2015): Annales Bogorienses
Publisher : BRIN

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Abstract

The continuation of new antibiotics exploration becomes an important research program in the world for pharmaceutical and agricultural applications. Marine filamentous bacteria such as actinomycetes have been widely used as an important biological tool to generate a variety of new secondary metabolites, such as antibiotic. The aim of this study was to obtain identified active compound and determine its antimicrobial activity. Isolation, identification, and antimicrobial activity assay of active compound produced by marine actinomycetes isolate A32 had been conducted. Production of active compound using isolate actinomycetes A32 was conducted involving glucose, yeast, peptone medium. The fermentation was carried out at 30 ºC for 5 days. The broth of supernatant was extracted using ethyl acetate. Purification of active compound used chromatography column and eluted stepwise with the chloroform and methanol solvents. Antimicrobial activity was monitored using agar disc diffusion, and microbial test was conducted by analyzing the samples diameter of clear zone towards Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 66923, and Candida albican BIOMCC 00122. Results of isolation and purification of active compound produced by actinomycetes isolate A32 show that this compound has a molecular weight of 503.1 g/mol with molecular formula C26H37N3O7. Furthermore, this compound was suspected as Madumycin II after analysis of spectrum using 1HNMR and COSY. The antimicrobial activity assay confirms that this active compound inhibited the growth of Staphylococcus aureus ATCC 25923 and Candida albican BIOMCC 00122.
EFFECT OF STORAGE TIME VARIATION OF ANDALIMAN FRUIT ON FREE RADICAL SCAVENGING AND PROLIFERATION INHIBITION ON MCF-7 CELLS Rosidah, Idah; Kusumastuti, Siska Andrina; Simanungkalit, Grace Sophiwati; Rahayu, Maya Damayanti; Nuralih, Nuralih; Martgrita, Merry Meryam; Kembaren, Roga Florida; Chaidir, Chaidir; Sunaryanto, Rofiq; Wibowo, Agung Eru
Berita Biologi Vol 23 No 2 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.3334

Abstract

Andaliman (Zanthoxylum acanthopodium D.C) is a plant widely used by the Batak people in North Sumatera. Several studies demonstrated that Andaliman fruit exhibits antioxidant activities and cytotoxic effects on cancer cells. Quality of Andaliman fruit could affected by post-harvest process including storage time. This research aims to analyze the compound, antioxidant activity and proliferation inhibition on MCF-7 cells of Andaliman fruit extracts (AFE) stored at varying storage time. A series of fresh 250 g Andaliman fruit sealed in a closed container and stored in the oven at 30ºC. After storage at 0, 24, 48, 72, 96, 120 and 144 hours, Andaliman fruit macerated using methanol and obtained successive extract yields of 4.12; 3.70; 12.79; 8.17; 8.15; 3.7 and 2.21. These extracts were analyzed for total phenolic, total flavonoids, free radical scavenging activity and proliferation inhibition on MCF-7 cells. The chemical compound analysis of AFE was performed by ultra performance liquid chromatography (UPLC). Results showed that the AFE stored at zero hours had higher total phenol content (47.32 mg gallic acid equivalent (GAE)/g of extract) and free radical scavenging activity (50.38%) at 500 µg/mL significantly compared to other extracts. The total flavonoid content of all extracts showed no difference level except for the extract at 120 hours of storage. The proliferation inhibition test on MCF-7 cells at 100 µg/mL showed that AFE stored at 72, 96 and 144 hours could inhibit MCF-7 cells above 50%. The storage time variation of Andaliman fruit may affect the total phenolic and flavonoid content, and also activity of free radical scavenging and proliferation inhibition on MCF-7. The UPLC analysis founded the major compound of AFE was predicted as α-sanshool. Analysis of chemicals substance in Andaliman fruit with varying of storage time need to be conducted to evaluated alteration of secondary metabolites contained in Andaliman fruit.
Co-Authors Agung Eru Wibowo Ahmad Marasabessy, Ahmad Bambang Marwoto BERTI HARIASIH HANDAYANI Chaidir Chaidir DIANA NURANI Edy Marwanta, Edy Efrida Martius Ema Damayanti Heru Agus Cahyanto, Heru Agus Idah Rosidah Khaswar Syamsu Mahsunah, Anis Herliyani Martgrita, Merry Meryam Mustofa Mustofa Nabilah, Siti Nuralih Nuralih, Nuralih Nurhayati Nurhayati Rahayu, Maya Damayanti ROGA FLORIDA KEMBAREN Sari, Selvi Ayu Simanungkalit, Grace Sophiwati Siska Andrina Kusumastuti