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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Nominal: Barometer Riset Akuntansi dan Manajemen Jurnal Penelitian dan Pengembangan Sains dan Humaniora (JPPSH) Jurnal Ilmu Dasar Jurnal Manajemen dan Keuangan JURNAL BIOLOGI INDONESIA Jurnal Ekonomi dan Bisnis JEMA: Jurnal Ilmiah Bidang Akuntansi dan Manajemen ANNALES BOGORIENSES Jurnal Analisis Bisnis Ekonomi Jurnal AKSI (Akuntansi dan Sistem Informasi) Jurnal Keperawatan Muhammadiyah International Journal of Social Science and Business EKUITAS (Jurnal Ekonomi dan Keuangan) Humanism: Journal of Community Empowerment (HJCE) Pediomaternal Nursing Journal Babali Nursing Research Medicra (Journal of Medical Laboratory Science/Technology) Indonesian Academia Health Sciences Journal Jurnal Akuntansi dan Keuangan Indonesia Tsaqofah: Jurnal Penelitian Guru Indonesia Jurnal Keperawatan Journal Healthcare Education Jurnal Pengabdian Masyarakat ILJ: Islamic Learning Journal Jurnal Pendidikan Tuntas Annales Bogorienses Jurnal Analisis Bisnis Ekonomi

Supatmi Supatmi

Jurusan Fisika, FMIPA Universitas Sebelas Maret Surakarta, Jl. Ir. Sutami No. 36 A, Surakarta 57126

Author-ID : 393450

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Growth and Proline Content of Irradiated in Vitro Shoots of Ubi Kuning Cassava Genotype Cultured at Different Temperatures Supatmi, Supatmi; Rahman, Nurhamidar; Hartati, N Sri
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Cassava (Manihot esculenta) is an important crop to food security under climate change due to its various tolerance mechanism under stress conditions. However, the sustainable growth of cassava in the field depends on many factors especially temperature. The objective of the research was to investigate the growth performances and proline contents of irradiated Ubi Kuning at dosage of 10 Gy, cultured in Murashige Skoog (MS) hormone-free solid medium for 4 weeks at three different temperature treatments i.e 25oC, 28oC and 30oC. Each treatment consisted of 3 clone explants with 5 replicates. Results show that growth performances of irradiated plantlets were better compared to that of non-irradiated plantlets in terms of plant height and number of leaves at all temperature tested. The best growth performances were obtained from irradiated plantlets grown under 30oC. The proline content of irradiated Ubi Kuning was high when they were grown under 25 oC and 30oC, implying that these plantlets had the possibility to tolerant to lower and higher-temperature condition. This study is initially useful to find out the growth ability of irradiated Ubi Kuning in response to lower and higher temperature.

Massive In Vitro Propagation Of Sandalwood Through Friable Embryogenic Callus Supatmi, Supatmi; Ardiyanti, Nurdiya; Rahman, Nurhamidar; Sudarmonowati, Enny
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Sandalwood (Santalum album), which belongs to Santalaceae family, is a commercially important tree in Indonesia due to its many application. However,its population has significantly depleted since the planting materials using conventional methods are difficult to be provided. This study was conducted to mass propagate sandalwood using in vitro methods through friable embryogenic callus (FEC). The somatic embryos were formed using leaves cultured in MS +0.5 mg/l +1 mg/l indole acetic acid (IAA), and MS +1 mg/l IAA + 0.2 mg/l kinetin as well as 0.5 MS+1 mg/l Gibberellic acid (GA3). Primary somatic embryos (PSE) and secondary somatic embryos (SSE) then formed friable embryogenic callus when they were repetitively transferred to MS +1.5 mg/l BAP + 1.2 mg/l kinetin every 3 weeks. The maturation and regeneration of FEC was best done in the MS +1.5 mg/l BAP + 1.2 mg/l kinetin for 4-8 weeks. The acclimatization of sandalwood plantlets can be best conducted in the medium containing soil, sand and compos in ratio of 1:1:1 with the companion plant Murraya paniculata, which gave the best percentage of survival rate and the lowest percentage of falling leaves.

Improved Regeneration, Acclimatization and Shoot Cutting Production of âGebangâ Cassava Derived from Irradiated In Vitro Shoots Supatmi, Supatmi; Sudarmonowati, Enny
ANNALES BOGORIENSES Vol 16, No 2 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Gebang is an Indonesian local genotype which has been selected as superior genotype for low amylose Â cassava. Prior to induction of new mutants of this genotype, series of research have been conducted to improve regeneration and acclimatization as well as shoot cuttings production of irradiated in vitro shoots. Four dosage treatments of gamma ray irradiation i.e. those of at 0; 0.2; 1.0; 1.5; 2.0 krad were applied to 32 in vitro shoots multiplied from apical shoots of plants in the field. The highest multiplication rate and acclimatized shoots were obtained from shoots irradiated with 0.2 krad multiplied on various level of BAP added on MS medium and resulted that MS supplemented with 1 mg/L BAP was the best medium. The phenotypic variation was observed in shoots irradiated with 1.0; 1.5 and 2.0 krad while the ones irradiated with 0.2 krad performed normal appearances. Subsequent production of propagated young stem cuttings called âratooning systemâ decreased after the second cycle of propagation especially in the survival rate of the ones irradiated with 0.2 krad. The findings lead to the opportunity to produce cassava propagules derived from irradiated in vitro culture at a higher amount using Gebang genotype as a model.Keywords: cassava Gebang, irradiated shoot, young stem cutting/ratooning , acclimatization, in vitro.

Identification of Differentially Expressed cDNA in Cassava under Drought Stress Using cDNA-RAPD Approach Hartati, N Sri; Supatmi, Supatmi; Aryaningrum, Pramesti Dwi; Sudarmonowati, Enny
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/72

Cassava is an important carbohydrate source that provides food security and alternative renewable energy development. This plant is naturally drought tolerant, but there is a wide variation within cassava genotypes in their ability to maintain high yield and starch production under drought stress. It has been reported that cassava clones with leaf retention or stay green trait can produce more total fresh biomass and high root dry matter compared to drought susceptible cultivars. The genetic, biochemical and molecular bases of stay green trait need to be understood to develop drought resistant cassava cultivars since water stress limits yield and starch production. Differential Display (DD)RT-PCR is a powerful technique for analyzing differences in gene expression. The method is based on the detection of the differentially expressed cDNAs from two or more samples. The main objective of this study was to identify differentially expressed cDNA in cassava under drought stress by employing a cDNA-RAPD approach. In this study, we used cassava genotype Ubi Kuning which was considered the most responsive to water insufficiency (45 days without watering). Leaf samples were collected from water stressed and well-watered plants at day 45. Among 11 random primers, OPB03 and OPH17 have identified differentially expressed cDNA in Ubi Kuning. Further characterization of these PCR products of expressed cDNA under drought stress may open possibility the development of cassava with improved drought resistance through genetic engineering and/or marker assisted selection (MAS).

Co-Authors Ab Latif, Rusnani Anandhita, Aries Chandra Andriyan, Okky Andriyan, Okky Anis Rosyiatul Husna, Anis Rosyiatul Ardiyanti, Nurdiya Ardiyanti, Nurdiya Aryaningrum, Pramesti Dwi Aryaningrum, Pramesti Dwi Avita Ervianti, Tantri Cartealy, Imam Civi Darma Sudata Wijaya Edi Iskandar, Edi Elyson , Vionna Natasya Eni Purwaningsih Enny Sudarmonowati Feviana, Dwi Lia Hani Fitriani, Hani Harijanto, Gabriella Stephanie Hartati Hartati Hartati, N Sri Hartati, N Sri ISKANDAR ZULKARNAEN SIREGAR Jannah, Faiqotul Meliniawaty, Elma Mukarromah, Nur Mundakir Muzamil, Akhmad N. Sri Hartati, N. Sri Nadhiroh, A'im Matun Nastiti Kartikorini, Nastiti Neogo Dwianka Idealfa Novita Kumalasari, Dian Perdana Sastra Primadani, Ines Aprilia Priyantini, Diah Purnomo, Parmadi Sigit Putri, Chyntia Meiliana Putri, Verlina Anggita Kartika Rahayu, Bety Agustina Rahman, Nurhamidar Rahman, Nurhamidar Reliani Reliani, Reliani Resiyati, Yuni Ridoh, Rasyid Rubi Awalia, Rubi Saputra, Husyin Sari Kusumastuti Sisilia Devina Permatasari, Sisilia Devina Siti Mardiyah Sri Setyowati Sukari Sukari, Sukari Suyatno Suyatno syafaati, Aditya Laily Syamsidah Rahmawati, Syamsidah Tursilowati, Sri Yuni Ulfah Juniarti Siregar Ulfah, Qamariyah Uswatun Hasanah Wasiah , Wasiah Wasiah, Wasiah Wulandari, Anisa Yayi Suryo Prabandari Yeterina Widi Nugrahanti Yuanita Wulandari, Yuanita Yumni, Fathiya Lutfil Yunita Prabawati, Chlara

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