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All Journal Agrotrop : Journal on Agriculture Science journal of internal medicine AGRITROP Journal of Agricultural Science and Biotechnology Jurnal Veteriner Bumi Lestari Ecotrophic, Journal of Environmental Science E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Biogenesis: Jurnal Ilmiah Biologi MNJ (Malang Neurology Journal) JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrica: Journal of Sustainable Dryland Agriculture Jurnal Ilmiah Teknologi Pertanian Agrotechno
Dewa Ngurah Suprapta
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Neuroscience Veterinary

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Journal : Journal of Agricultural Science and Biotechnology

 1 
POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFER TANAMAN PADI SEBAGAI AGEN HAYATI UNTUK MENGHAMBAT PERTUMBUHAN JAMUR Pyricularia oryzae, PENYEBAB PENYAKIT BLAS PADA TANAMAN PADI Valente Quintao; Dewa Ngurah Suprapta; I Gede Rai Maya Temaja; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Rice (Oryza sativa L.) is one of the staple foods for more than 60 percent of world population. Indonesian people generally are still depending on the availability of rice to fulfill Indonesian food demand. The average of rice productivity in Indonesia is 4.56 ton/ha which is relatively lower when compared with other rice growing countries, such as Australia 8.22 ton/ha;  Japan 5.85 ton/ha and China 6.06 ton/ha. One of the causes for the low productivity of rice in Indonesia is the occurrences of rice blast disease caused by Pyricularia oryzae.  To control this disease, the farmers are still rely on the use of synthetic fungicides however this measure can not effectively control the disease,  and potentially cause the health and environmental problems. It is necessary to find an alternative measure  that save to human health as well as friendly to the environment. This study was  done to evaluate the potential use of rhizobacteria isolated from rhizospheres of rice as antagonist against Pyricularia oryzae the  cause of rice blast disease.  The result showed that five isolates of rhizobacteria namely  Xanthomonas lumininescens isolate Ch3Da, Serratia liquefaciens isolate Gh13DaB, Enterobacter agglomerans isolate Gg14DtB, Enterobacter agglomerans isolate Ch2Da, and Enterobacter agglomerans isolate Ch4BaB significantly (P<0.05) inhibited the growth of Pyricularia oryzae on potato dextrose agar (PDA) medium with inhibitory activity varied from 39.46% to 46.66%. All of these isolates produced extra cellular substances which probably responsible for the inhibitory activity. This result suggested that five isolates of rhizobacteria tested in this study can be further developed as bio-control agent to reduce the development of rice blast disease.
ISOLASI DAN IDENTIFIKASI JAMUR PENGHASIL MIKOTOKSIN PADA BIJI KAKAO KERING YANG DIHASILKAN DI FLORES Emilia Simpllisiu Ake Wangge; Dewa Ngurah Suprapta; Gusti Ngurah Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

A study entitled “Isolation and Identification of Mycotoxin-producing Fungi on Cocoa Beans Produced in Flores” was done in order to know the population and species of fungi and to know the concentration of mycotoxin in the dried cocoa beans. The dry cocoa beans used in this study were collected from several locations in Flores. Isolation of the fungi was done using Sabouraud Dextrosa Agar (SDA), while the determination of mycotoxin concentration was done based on Enzym Linked Immunosorbent Assay (ELISA). Results of this study showed that,  there were several fungi were found in the dried cocoa beans produced in Flores, namely Aspergillus niger, A. flavus, A. parasiticus, A. fumigatusandMucor sp. All tested dried cocoa beans contained aflatoxin B1, where the highest aflatoxin B1 concentration was found in the fermented beans from West Manggarai with the concentration at 3.65 ppb, while the lowest concentration was 2.21ppb, which was found in the cocoa beans from Nagekeo. The highest concentration of ochratoxin A was 0.38  ppb, which was found in the cocoa beans from West Manggarai, while no ochratoxin A was detected in the cocoa beans from Ende.
UJI ANTAGONIS BAKTERI ENDOFIT TERHADAP Colletotrichum scovillei PENYEBAB PENYAKIT ANTRAKSONA PADA CABAI BESAR (Capsicum annuum L.) I Gede Karta Satria Wibawa; Dewa Ngurah Suprapta; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Colletotrichum scovillei is a newly discovered species causing anthracnose disease on chilli pepper (Capsicum annuum L.) in Bali. With the increasing reports of Colletotricum spp. resistance against chemical fungicide, biological control is taken as the alternatives to control this disease. In this study a total of 69 endophytic bacteria isolates was recovered from different solanaceae plants samples collected from nine regions of Bali and screened for the antagonism againstC. scovillei. From the potential 15 candidates of isolates with inhibition activity above 90%, hypersensitivity test were taken on tobacco leaf, and isolate CBCKB1 was chosen for further test. The CBCKB1 isolate was identified as Bacillus amyloliquefaciens based on molecular identification. Detached fruit bioassay proved that isolate CBCKB1 formula was effective in reducing the disease percentage and disease severity of anthracnose disease on chilli pepper.
POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFIR TANAMAN GRAMINAE NON-PADI UNTUK MEMACU PERTUMBUHAN BIBIT PADI Ni Made Intan Maulina; Khamdan Khalimi; Gusti Ngurah Alit Susanta Wirya; Dewa Ngurah Suprapta
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Rice (Oriza sativa L.) is the main food for Indonesian people, however the rice production is not enough to fulfill all national demand. Rice productivity in Indonesia was about 4,5 ton/ha, relatively low when compared to other rice growing countries such Australia, Japan, and China. It is necessary to find a measure to increase the rice productivity in Indonesia. One of the Measure is the use of rhizobacteria isolated from the rhizozpheres of the plants belongs to the family Graminae. This study was done in order to find potential rhizobacteria from rhizospheres of five plant species belong to the family Graminae, namely Cymbopogon ciatrus, Imperata cylindrica, Saccharum officinarum, Pennisetum purpureum and Schizostachum mosum. Result of this study showed that 10 isolates of rhizobacteria obviously increased the number of lateral roots of rice seedling cultivar Ciherang. The rice seedling treated with isolate Al7Kla produced the highest number of lateral roots when compared to other isolates. This isolate may be used to promote the rice growth in order to increase the yield.
UJI EFEKTIVITAS RIZOBAKTERI SEBAGAIA GEN ANATGONIS Sclerotium rolfsii PENYEBAB PENYAKIT BUSUK BATANG PADA TANAMAN KACANG TANAH (Arachis hypogea L.) Ni Kadek Desy Andya Dewi; Dewa Ngurah Suprapta; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Groundnut (Arachis hypogea L.) is one of important commodities in Indonesia, however the production of groundnut is still relatively low which ispartly because of the plant disease i.e. stem rot disease caused by Sclerotium rolfsii. Rhizobacteria is one of the solutions to be used as the biological controlagent against S. rolfsii. The aim of this study is to know the effectiveness of rhizobacteria to control stem rot disease caused by S. rolfsii. The results the studyshowed that among 130 isolates of rhizobacteria tested against S. rolfsii, 11 isolates showed inhibitory activity, and among them one isolate, BjTk11 fromexposed the highest inhibitory activity (96.09%) against S. rolfsii. On a green house experiment, treatment with formula of isolate BjTk 11 at doses of 5 g, 10 g,15 g and 20 g per polybag effectively controlled the pre emergence damping off (disease incidence = 0%) while the disease incidence on control was 23.5%. Nopost emergence damping off symptom was observed on groundnut treatment with formula of isolate BjTk11, while the disease incidence on control was 75%. The number of sclerotia in soil of control was 79/gram of soil which is obviously higher than those of treatments. The mode of action the rhizobacteria isolatesBjTk11 in inhibiting the growth of S. rolfsii is through antibiosis where the rhizobacteria produce the antifungal compound Butanedial, R - (-) - 1,2propanediol, 2,3-Butanediol, Isopropyl Alcohol, Acetic acid, anhydride with formic Acid , 2-bromohexane, 4,5-Diamino-6-hydroxypyrimidine, 4H-Pyran-4one, 3-hydroxy-2-methyl and Cyclotrisiloxane, hexamethyl-. The presence of these compounds probably closely related to the inhibitory activity against S. rolfsii.
POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFER TANAMAN LEGUMINOSAE UNTUK MENGENDALIKAN JAMUR Sclerotium rolfsii PENYEBAB PENYAKIT REBAH KECAMBAH PADA TANAMAN KEDELAI Komang Adi Mahartha; Dewa Ngurah Suprapta; Gusti Ngurah Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Damping off disease caused by Sclerotium rolfsii is an important disease of soybean, because soybean plant will die after attacked by pathogen. This pathogen can survive for a long time in the soil by sclerotia form. Some of rhizobacteria isolated from the rhizospheres of Leguminosae plants were known could control soil-borne pathogens. This study aims to test rhizobacteria isolated from the rhizosphere of Leguminosae plants that able to suppress the growth of S. rolfsii. Total of 11 isolates of 112 isolates rhizobacteria that could be isolated from Leguminosae plants had antifungal activity against S. rolfsii. Inhibition test of 11 isolates of rhizobacteria against S. rolfsii showed that three isolates had the highest ability to inhibit the growth of S. rolfsii namely isolates KtD1, KtD6, and KtB3. This result suggested that three isolates of rhizobacteria tested in this study can be further developed as bio-control agent to reduce the development of damping off disease on soybean.
Co-Authors Agung Bagus S. Satyarsa Agung Wiwiek Indrayan Agus Selamet Duniaji ALPIN JUAN HAGATA PINEM Anak Agung Ngurah Subawa BERTA BR GINTING Emilia Simpllisiu Ake Wangge Gitari, Ni Made Gusti Ngurah Alit Susanta Wirya Hadis Jayanti HENRI PAN BAGUS MULIA NAPITUPULU I B Yoga I Dewa Made Sukrama I G. R. M. TEMAJA I GEDE KARTA SATRIA WIBAWA I Gede Karta Satria Wibawa I Ketut Suada I MADE ADNYANA I Made Jawi I Made Sudana I MADE SUDARMA I Putu Eka Widyadharma I WAYAN GENTA ARIAWAN I Wayan Niryana I Wayan Sumardika Jayanti , Hadis K Agung Sudewa Ketut Suata Khamdan Khalimi Komang Adi Mahartha l MADE SUDARMA LASTRI APRIANI Leana, Ni Wayan Anik Losen Adnyan M. Sudiana Mahendra Made Agus Hendrayana MADE SUSUN PARWANAYONI Meitini Proborini Wahyuni Ni Kadek Desy Andya Dewi NI KOMANG SRI BAWANTARI NI LUH GEDE SRIYANTI NI LUH PUTU SONIA SAVITA DEWI Ni Made Intan Maulina Ni Nyoman Puspawati Ni Putu Ristiati NI WAYAN SUNITI NOVA SULIYANTI SIANTURI Nyoman Darsini PARDIANTA PATRISIUS SINAGA Raka-Sudewi A. A. SANG KETUT SUDIRGA Sanjaya, Feliani Sukmawati Sukmawati Sukmawati Sukmawati Thomas Eko Purwata Unedo, Dion Valente Quintao