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Journal : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

Uji Efektivitas Fungisida Alami dan Sintetis dalam Mengendalikan Penyakit Layu Fusarium pada Tanaman Tomat yang Disebabkan oleh Fusarium oxysporum f.sp. lycopersici LASTRI APRIANI; DEWA NGURAH SUPRAPTA; I GEDE RAI MAYA TEMAJA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT The Test of Natural and Synthetic Fungicides Effectiveness in Controlling Fusarium Wilt of Tomato Plants Caused by Fusarium oxysporum f.sp. lycopersici Fusarium wilt caused by Fusarium oxysporum f.sp. lycopersici is one of important diseases in tomato plants. The emersion of the disease can result in huge loss for farmers. The use of synthetic fungicides that has been widely utilized all this time by farmers to control diseases caused by Fusarium oxysporum f.sp. lycopersici has adverse environmental impacts, therefore it is required biological fungicide that is more environmentally friendly. This study is purposed to testing the effectiveness of some natural fungicides and synthetic fungicide, is like a betel leaf, Trichoderma harzianum fungus, Klebsiella pneumoniae, and benomyl for suppress F. oxysporum f.sp. lycopersici and restrained Fusarium wilt diseases . The result of this study shows that the biological fungicide of betel leaf extract can inhibit the pathogenic F. oxysporum f.sp. lycopersici by 76.11 % and the percentage of inhibition potency of other benomyl fungicides reaches 61.11 % , while Klebsiella pneumoniae is by 54.42 % , and 20.98 % for Trichoderma harzianum on PDA medium. The result of field test shows that the extract of betel leaf can suppress Fusarium wilt by 5 % , T. harzianum , benomyl and K. pneumoniae are able to suppress Fusarium wilt respectively by 2.5 %. The use of T. harzianum and K. pneumoniae as biological control has the same effectiveness with synthetic fungicide in order to suppress Fusarium wilt.   Keywords: fusarium wilt, fusarium oxysporum f.sp. lycopersici, biological fungicide
Uji Keefektifan Rizobakteri dalam Menghambat PertumbuhanJamurColletotrichum spp. Penyebab Antraknosa pada Cabai Merah (Capsicum annuum L.) NI LUH GEDE SRIYANTI; DEWA NGURAH SUPRAPTA; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Effectiveness of Rhizobacteria to Inhibit the Growth of Colletotrichum spp. the Cause of Antracnose on Red Chilli (Capsicum annuum L.) Colletotrichumspp. is one of pathogenic fungi that cause significant losses on red chilli. The use of rhizobacteria is considered as an alternative to control the growth of the fungus. The use of bio agent control is considered as selective and environmentally friendly. The aim of this study is to know the effectiveness of rhizobacteria and their filtrates in suppressing the growth of Colletotrichumspp. Results of this study showed that treatment with Pseudomonas fluorescens isolate TBZA on PDA medium could suppress the growth of Colletotrichumspp. by 94% when compared to control 14 days after inoculation. Treatment with filtrate of P. fluorescens isolate TBZA could suppress the anthracnose disease indicated by the lower disease incidence viz 32%. It is necessary to conduct further experiment under field condition to know the effectiveness of P. fluorescens isolate TBZA to suppress the growth of Colletotrichumspp. In addition isolation and identification of antifungal compounds in the filtrate is necessary to be done. Keywords: rhizobacteria, biologicalagents, and Colletotrichumspp.
Uji Aktivitas Antijamur Bacillus siamensis C7B terhadap Jamur Colletotrichum scovillei Penyebab Penyakit Antraknosa pada Tanaman Cabai Besar (Capsicum annuum L.) PARDIANTA PATRISIUS SINAGA; KHAMDAN KHALIMI; DEWA NGURAH SUPRAPTA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.4, Oktober 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Test of Antifungal Activity of Bacillus siamensis C7B Against Fungus Colletotrichum scovillei Causing Anthracnose Disease on Chili Pepper (Capsicum annuum L.) Colletotrichum scovillei is one of the species of fungi that causes anthracnose disease on chili peppers. The Purpose of this study was to determine the potential of B. siamensis C7B in inhibiting the growth of the fungus C. scovillei that causes anthracnose disease in large chili (C. annuum L.). This study was conducted in vitro by testing the antifungal activity of Bacillus siamensis C7B against three isolats of C. scovillei namely TBCR, SGCR, and GRCR on potato dextrose agar (PDA) and potato dextrose broth (PDB) media. The test results indicate that inhibition of the bacteria B. siamensis C7B able to inhibit the growth of fungi isolates of C. scovillei namely TBCR, SGCR, and GRCR with the percentage of inhibition respectively by 94,91%, 92,66%, and 91,47% when compared to the control on the observation of 21 days after inoculation. The inhibition test results of B. siamensis C7B filtrate at a concentration of 50% showed that the B. siamensis C7B filtrate was able to inhibit the growth of C. scovillei TBCR isolates with a percentage of inhibition activity of 90,27% when compared to control. The results of inhibition test of B. siamensis C7B on the formation of C. scovillei biomass of isolate TBCR showed that B. siamensis C7B was able to inhibit the formation of fungal biomass by 73,81% when compared to control. Further study is needed to evaluate the effectiveness of B. siamensis C7B to control anthracnose disease in vivo on chili pepper.
Pemanfaatan Aeromonas hydrophila Untuk Mengendalikan Penyakit Layu Fusarium Pada Beberapa Varietas Sorgum Manis (Sorghum bicolor (L.) Moench I WAYAN GENTA ARIAWAN; DEWA NGURAH SUPRAPTA; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.2, April 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The Use of Aeromonas hydrophila to Control Fusarium Wilt Disease on Several Varieties of Sweet Sorghum (Sorghum bicolor (L.) Moench) Objective of this study was to test the effectiveness of A. hydrophila to control wilt disease caused by Fusarium sp. on several varieties of sweet sorghum. Results of this study showed that application of rhizobacteria affected the growth and the yield of sweet sorghum. Under in vitro condition, treatments with A. hydrophila isolate KtBlt2 could suppress the growth of the Fusarium sp. with inhibitory activity 76.06% when compared to control. Under field condition treatment P6 (treatment wilt A. hydrophila on variety FS-501) could suppress Fusarium wilt disease with the lowest disease incidence by 3.24%. It is necessary to test the stability of A. hydrophila as biocontrol agent against Fusarium sp. Keywords : A. hydrophila, Fusarium sp., sweet sorghum
Uji Efektivitas Ekstrak Daun Cengkeh (Syzygium Aromaticum L.) Terhadap Phytophthora Palmivora Penyebab Penyakit Busuk Buah Kakao (Theobroma Cacao L.) NI LUH PUTU SONIA SAVITA DEWI; DEWA NGURAH SUPRAPTA; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.4, Oktober 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Effectiveness of Clove Leaf Extract (Syzygium aromaticum L.) againstPhytophthora palmivora the Cause of Cocoa Fruit Rot (Theobroma cacao L.) Cocoa fruit rot caused by Phytophthora palmivora is one of the main diseases that can greatly reduce the yield of cocoa production in the world. Therefore it isnecessary to control the disease in an environmentally friendly way. One of which isusing botanical pesticides. Clove leaf is one of the plants which extract can be usedas a fungicide. This study aimed to examine the effectiveness of clove leaf extract onP. palmivora the cause of cocoa fruit rot. MIC test and colony test was done using 10extract concentrations namely 0,1; 0,2; 0,3; 0,4; 0,5; 0,6; 0,7; 0,8; 0,9; 1,0 and 0,0%(control). The in vivo test uses 6 extract concentrations of 0,5; 0,6; 0,7; 0,8; 0,9; 1,0and 0,0% (control). The results showed the MIC (minimum inhibitory concentration)of clove leaf extract to suppress the growth of P. palmivora was 0.5% which means itis feasible to be used as a botanical pesticide. Under in vivo condition, clove leaf extractinhibited the growth of fungal colonies and infection and can be used as fungicide tosuppress the growth of P. palmivora. The extract concentration of 1% prevented thedamage of cocoa fruit caused by P. palmivora by 100%.
Uji Aktivitas Antijamur Ekstrak Biji Keben (Barringtonia asiatica (L.) Kurz) terhadap Curvularia verruculosa Penyebab Penyakit Bercak Curvularia pada Tanaman Padi (Oryza sativa L.) I GEDE KARTA SATRIA WIBAWA; DEWA NGURAH SUPRAPTA; KHAMDAN KHALIMI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.3, Juli 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Antifungal Activity of Keben (Barringtonia asiatica (L.) Kurz) Seed Extract against Curvularia verruculosa the Causal Agent of Curvularia Leaf Spot Disease on Rice (Oryza sativa L.)This research was conducted to determine the effectiveness of keben (Barringtonia asiatica (L.) Kurz) seed extract to control Curvularia verruculosa the causal agent of curvularia leaf spot disease on rice. The extract concentration of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9% and 1.0% was tested on in vitro test to determine the minimum inhibitory concentration (MIC) and inhibition percentage. Crude seed extract was partitioned and analyzed using GC-MS to determine the active antifungal compound. Concentration of 0.6%, 0.7%, 0.8%, 0.9% and 1.0% is tested on in vivo test in a greenhouse to determine the effectiveness of keben seed extract formula to control the curvularia leaf spot disease on rice. The result shows that the MIC of keben seed extract was 0.5% with inhibitory percentage that increases from 0.1% to 0.5% and cause 100% inhibitory on 0.6% concentration and above. GC-MS analysis showed antifungal compound such as Butyl Hydroxy Toluene; Dodecanoic acid, methyl ester; Methyl tetradecanoate; Benzoic acid 2, 5-bis (trimethylsiloxy)-trimethylsilyl ester; 1-Tetradecanol; Hexadecanoic acid, methyl ester; Octasiloxane, 1, 1, 3, 3, 5, 5, 7, 7, 9, 9, 11, 11, 13, 13, 15, 15-hexadecamethyl-; 13-Docosenoic acid, methyl ester (Z)- and 1, 2-Benzenedicarboxylic acid, dioctyl ester are present in keben seed. Greenhouse test showed that keben seed extract can suppress curvularia leaf spot disease from 25.16% to 2.57%.
Uji Efektivitas Agen Hayati Dari Rizosfer dan Filosfer Tanaman Solanaceae untuk Mengendalikan Penyakit Antraknosa pada Tanaman Cabai Rawit (Capsicum frutescens L.) HENRI PAN BAGUS MULIA NAPITUPULU; KHAMDAN KHALIMI; DEWA NGURAH SUPRAPTA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.1, Januari 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Effectiveness of biological agents from Solanaceae plants rhizosphere andfilosphere to control Antrachnose disease in Chili Pepper Plants (Capsicum frutescens L.). The disease antrachnose caused by the fungus Colletotrichum truncatum canattack the chili fruit at any stage, but it will not attack the leaves or stems. The use ofbiological agents from the rhizosphere and filosphere is a viable alternative to controlthe anthracnose disease. Biological agents work selectively and better for theecosystem. The goal of this research is to identify the effectivenes of biological agentsfrom rhizospheres and filospheres solanaceae plant to control C. truncatum that causesantrachnose disease in Chili Pepper Plants (Capsicum frutescens L.). This researchtakes place in vitro and the field this research shows that TmFr4 and TrRr7 treatmentcan inhibit the growth of C. truncatum in vitro with a high percentage of inhibition90,58 % and 91,23 %. The TrRr7 bacterial isolate can inhibit the anthracnose diseasefrom 95,23% to 64,23 % and able to reduce the intensity from 66,59 % tob 39,06 %while TmFr4 fungal isolates can inhibit the desiase from 95,23 % to 68,81 % andreduce its intensity from 66,59 % to 47,03 % base on the field test. Further study isneeded to analyze the stability of TmFr4 fungal isolate and TrRr7 bacterial isolatebiological control of C. truncatum in the field.
Uji Antagonistik Bacillus siamensis dan Paenibacillus polymyxa Terhadap Colletotrichum gloeosporioides KLCR2 Penyebab Penyakit Antraknosa pada Buah Cabai Rawit (Capsicum frutescens L.) NI KOMANG SRI BAWANTARI; DEWA NGURAH SUPRAPTA; KHAMDAN KHALIMI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.3, Juli 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Antagonistic test of Bacillus siamensis and Paenibacillus polymyxa against the growth of Colletotrichum gloeosporioides the cause of Antracnose disease in cayenne pepper (Capsicum frutescent L.) Colletorichum gloeosporioides is one of latent pathogens that can infect several agricultural crops. This pathogenic fungus is very detrimental to the production of cayenne pepper (Capsicum frutescens L.). The purpose of this research was to determine the ability of Bacillus siamensi and Paenibacillus polymyxa in inhibiting the growth of C. gloeosporioides KLCR2 causes of anaplosal disease. Research was conducted in Biopesticide laboratory. Results showed that treatment with antagonistic bacteria significantly (P<0.05) inhibited the growth of C. gloeosporioides on potato dextrose agar (PDA) medium. Treatment with B. siamensis was able to inhibit the growth of C. gloeosporioides with inhibitory activity by 96.18%. While for the treatment with P.polymyxa the inhibitory activity was 84.79%. Treatment with B. siamensi depressed the biomass of C. gloeosporioides KLCR2. In this study, treatment with cell-free filtrate of B. siamensis at concentration of 50% was able to suppress the development of C. gloeosporioides with a percentage of inhibitory activity by 94.15%. Further study is necessary to evaluate the ability of the B. siamensis to inhibit the development of C. gloeosporioides KLCR2 in vivo on cayenne pepper fruit.