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All Journal AGRIVITA, Journal of Agricultural Science Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan TERRA : Journal of Land Restoration Akta Agrosia
Eko Suprijono
Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Bengkulu
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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IDENTIFICATION OF M4 GAMMA IRRADIATED MAIZE MUTANT BASED ON RAPD MARKERS Rustikawati, Rustikawati; Suprijono, Eko; Romeida, Atra; Herison, Catur; Sutjahjo, Surjono H.
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i2.102

Abstract

Gamma irradiation to induce mutation in plant has been used intensively since several decades ago.  On maize, 275 Gy gamma irradiation have been known to increase genetic variability indicated by their morphological variation.  Identification on genetic changes by molecular technique is important to answer whether there is mutation happening on DNA level of the plants.   The objective of this research was to identify RAPD marker polymorphism on gamma irradiation mutants compared to their parents.  The initial step of the research was to select random primers could positively amplify the maize DNA.  The result showed that selection on 60 random primers yielded 15 primers that positively amplified the maize DNA.  Amplification on both mutants and their parents by those 15 selected primers indicated that only 5 primers yielding polymorphism between mutants and their parents.  Polymorphisms on mutant G1, G3 and G6 were detected on one locus, meanwhile on mutant G7, G8 and G9 were on two loci.
Rooting of Mini-Cuttings of guava cultivars (Psidium guajava L.) Treated with IBA under Misting Irrigation Supanjani, Supanjani; Rahayu, Hatari; Suprijono, Eko; Romeida, Atra; Yulian, Yulian
Akta Agrosia Vol 23, No 1 (2020)
Publisher : Faculty of Agriculture, The University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/aa.23.1.27-32

Abstract

       Guava (Psidium guajava L.) is a plant whose fruit is very popular in Indonesia because it contains high vitamin C and its leaves can be used as herbal medicine. Orchard expansion of selected cultivars and fruit production of guava can be achieved vegetatively through  propagation by using mini-cuttings with the use of Indole Butyric Acids (IBA). A study was conducted to compare rooting success of mini-cuttings of herbaceous stem of four guava cultivars with IBA treatment. Four guava cultivars tested were Bengkulu Round, Getas, Crystal, and Bangkok.  Mini-cuttings were immersed in IBA solution at 0 ppm or 1000 ppm. Cultivar and IBA treatments were arranged factorially in a randomized complete  block design with  3 replications, each consisting of 25 cuttings. Variables observed included percentage of original-leaf shedding, percentage of emerging shoots, percentage of sprouting cuttings, new leaf number, root number, root length, and percent of rooted cuttings. The results demonstrated that cultivars affected rooting percentage, sprouted percentage, percentage of original-leaf fall, and number of root.  Getas had highest rooting and sprouting percentages, and showed lowest number of  original-leaf shedding; whereas, Bangkok demostrated greatest number of root.  IBA treatment increased the number of root and the number of original-leaf shedding, but reducing rooting percentage of mini-cutting. There was no interaction between cultivar and IBA teratment.
Growth and Yield of Corn Plants Against Vermicompost Dosage in Degraded Ultisols Zikri, Ahmad; Widodo; Utami, Kartika; Anggraini, Septiana; Suprijono, Eko
TERRA : Journal of Land Restoration Vol. 6 No. 2 (2023)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/terra.6.2.57-64

Abstract

Ultisols has prominent weaknesses and problems such as high acidity levels, low water absorption capacity, and low nutrient content such as N, P, K, Ca, and Mg, so it has the potential to inhibit plant growth. One way to overcome Ultisols soil problems is to use vermicompost fertilizer. Corn is one of the plants that is suitable for cultivation using vermicompost fertilizer. The research aims to obtain the best dose of vermicompost for corn growth and yield in degraded Ultisol soil. The research was conducted in Sri Kuncoro Village, Pondok Kelapa District, Central Bengkulu Regency, Bengkulu Province. The design used a single-factor Complete Randomized Block Design (RCBD) consisting of 5 treatment levels and five replications to obtain 25 experimental plots of 0, 7.5, 15, 22.5, and 30 tons ha-1. Data were analyzed using Analysis of Variance (ANOVA) at a 5% level and continued with the Least Significant Difference (LSD) test at a 5% level. The research results showed that the dose of vermicompost significantly affected the variables of plant height, number of leaves, number of leaves, and plant dry weight. The best vermicompost treatment dose was 22.5 tons ha-1 with plant height 254.85 cm, number of leaves 13.26, stem diameter 24.90 mm, leaf area 811.49 cm2 , cob length with husk 24, 93 cm, cob length without husk 17.26 cm, cob weight with husk 218.19 g, cob weight without husk 194.26 g, dry seed weight of plant samples 153.33 g and Dry seed weight per ha 9.1 tons.
Evaluation of the application of NPK liquid fertilizer on the growth performance and yield of the UBPR-6 rice line in shallow swampy areas Sumardi, Sumardi; Chozin, Mohammad; Widodo, Widodo; Suprijono, Eko; Vadillah, Syavirah
Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Vol 22 No 2 (2024): Jurnal Agroqua
Publisher : University of Prof. Dr. Hazairin, SH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32663/ja.v22i2.4764

Abstract

Swamp land, especially shallow lowland swamps, has great potential and also has strategic value for the development of agricultural businesses, especially rice, to encourage the realization of a sustainable food self-sufficiency program. Fluctuations in water levels, which limit the application of fertilizer by spreading, can be reduced by the foliar fertilization method.  This research aims to evaluate the application of NPK liquid fertilizer on the growth performance and yield of the UBPR-6 rice line in shallow swampy areas. The experiment was carried out September 2022-January 2023 in shallow swampland at the Faculty of Agriculture, Bengkulu University. Using a Divided Plot design which places the NPK liquid fertilizer concentration (3.%, 6%, 9% and 12% as the main plot and application frequency (1, 2 and 3 times) as a subplot, with 3 replications. The results showed that high The plants were not affected by the application of liquid NPK fertilizer, but flowering accelerated along with increasing the concentration and frequency of fertilizer application. A concentration of 9% gave the best results with a yield of 3,505 kg per plot (6.25m2) or equivalent to 4.2 tons per hectare.
Performance of Lettuce (Lactuca sativa) Grown under Ultisol Subsoil with Chicken Manure and Dolomite Viani, Reza Junita Okta; Handajaningsih, Merakati; Suprijono, Eko; Marwanto
Akta Agrosia Vol 27 No 1 (2024)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/aa.27.1.1-6

Abstract

Lettuce is a horticultural plant with a short harvest time that is a popular vegetable choice for planting in polybags in urban areas. Planting media for cultivating plants in  polybags vary widely. The use of nutrient-poor and acidic subsoil in Ultisols as a planting medium requires additional organic matter and lime that are suitable for the plants. This study evaluated the use of subsoil as a medium for growing lettuce by adding เว็บสล็อต | R4shub chicken manure and dolomite. The Completely Randomized Design was used, which consisted of two factors and 6 replications. The first factor was the dose of chicken manure consisting of: 0 ton/ Ha, 10 tons/Ha (38g /polybag), 20 tons/ Ha (76g/ polybag), and 30 tons/ Ha (115g /polybag). The second factor was the dolomite dose, consisting of no dolomite, 1xAl-exch, and 2xAl-exch. A dose of 20 tons /Ha of chicken manure combined with   1xAl-dd of dolomite increased the growth of plants. The treatment of chicken manure at 20 tons/Ha showed growth and yield of lettuce that were as good as a treatment dose of 30 tons/ Ha. The highest fresh weight (123.05g) was obtained from 30 tons/Ha-chicken manure treatment. The appropriate dose of dolomite for lettuce growth and yield was 1xAl-exch, which resulted in a plant fresh weight of 121.12 g. Subsoil is suitable to be used as a medium for planting lettuce in polybags by adding chicken manure or dolomite.  Keywords: organic matter, marginal soil, leafy vegetable, container veg production
Potensi Produksi Enam Hibrida Jagung pada Ultisol di Provinsi Bengkulu Suprijono, Eko; Rustikawati, Rustikawati; Romeida, Atra; Gustian, Meko
Akta Agrosia Vol 18 No 2 (2015)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4289.503 KB)

Abstract

Ultisol is one of marginal land type quite extensively presence in Indonesia, including the province of Bengkulu. The use of varieties tolerance to acidity stress is the best manner to utilize of this type of land with relatively low cost and environmentally friendly. The objective of this research was to evaluate theyield potential of six maize hybrids developed to soil acidity tolerance. The experiment was conducted at the Experimental Farm Medan Baru, Kandang Limun Village, Muara Bangkahulu District, Bengkulu City, from August to November 2015. The experiment was arranged in a randomized completely block design with four replications. As the treatment were newly developed maize hybrids, namely CT5, CT8, CT9, CT13, CT14 and CT15. The variables measured were cob length, cob diameter, 100-seed weight and the estimated yield/ha. Qualitative variables supporting to corn yield were the cob position, seed color, seed type and seed row arrangement. The results indicated that the hybrid CT8 showed the highest estimated yield in Ultisol among hybrids evaluated, with the estimation of 6.5 tons per hectare. Qualitatively, theseeds of hybrid CT8 were a horse tooth type, pale yellow color, and stright line arrangement.
Pengujian Berbagai Tipe Tanam Jajar Legowo terhadap Hasil Padi Sawah Sari, Dia Novita; Sumardi, Sumardi; Suprijono, Eko
Akta Agrosia Vol 17 No 2 (2014)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.29 KB)

Abstract

Planting wetland paddy by setting row space could be a method to increase rice production. Legowo system is row planting method where we are able to apply different row types on paddy field. The objective ofthis research was to identify the best row planting type of ‘Jajar Legowo’ on growth and yield of wetland paddy. Six row plantings, including 2:1 type (means 2 rows are splited by wide space/ legowo), 3:1 type, 4:1 type, 5:1type, 6:1 type, and 7:1 typ, were tested in a Randomized Complete Block Design with 3 replications Results of the experiment revealed that the highest growth and yield as showed by total number of tillers (28), number ofproductive tillers (25), dry-mill rice (66.16 g per plant), and yield difference of dry-mill rice (50.43% per plot) was found at row planting type 2:1. Therefore, row planting type 2:1 can be selected as the best row.
Effect Branch Pruning To Establishment On Shoot Growth and Flower Stimulation On Guava Nafisah, Dio; Supanjani, Supanjani; Suprijono, Eko
Akta Agrosia Vol 22 No 2 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

GuavaCrystalsarefromTaiwan,released byministryofAgricultureDecreeNo.540/Kpts/SR.120/9/2007.GuavaCrystalvarietiesare very prospective forcommercial developmentbecauseitcanproduce   throughtheyearandhasahighvitaminCcontent. Increasingpublicawarenessoftheimportanceofconsumingfreshfruitforhealthincrease the market demandforfreshfruit.Butthe highdemandforfreshguavafruitisnotsupported bytheproduction offreshfruiteveryyear.Continueddecliningguavaproductionrequires theimprovementofcultivationtechniquessothat   thequalityandquantityofguavafruit increases. ThisneedstoImprove ofcultivationtechniquesformeetthe needsoffreshguava. One ofsimplestcultivationtechniquestoimprovethe yieldofCrystalguavaispruning. This research intendtodetermine the effectofcroplengthonthe growthoflateralshootsandthe flowering of Crystal guava.ThisresearchwasconductedinDecember2016untilMarch2017at guavaplantation locatedinBentiringSub-ProvinceofBengkuluProvince.ResearchusedRAKLwithone singlefactor.The factorislengthofpruning,whichcomprisedoffourlevels10cm,20cm,30 cm,40cmandcontrol.The resultsofthisstudyindicatedthatthe numberofshootsmost grownonbrancheswith 40cmlongtrimmingof 2.87shoots.Mostflowersaregeneratedon brancheswithcuttinglengthsof30cm,40cm andnottrimmed.Butbrancheswith30cm long cutsexperiencedthe mostnumberoffallenfruitswitha95% percentage ofunshelled branches.Themostfruit isproducedonbranchesthatarenotprunedthatisasmuchas8.7 will fruit.Keyword:Guava Crystals, Long Prune, Lateral Shoots, Flowering
Rooting of Mini-Cuttings of guava cultivars (Psidium guajava L.) Treated with IBA under Misting Irrigation Supanjani, Supanjani; Rahayu, Hatari; Suprijono, Eko; Romeida, Atra; Yulian, Yulian
Akta Agrosia Vol 23 No 1 (2020)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

       Guava (Psidium guajava L.) is a plant whose fruit is very popular in Indonesia because it contains high vitamin C and its leaves can be used as herbal medicine. Orchard expansion of selected cultivars and fruit production of guava can be achieved vegetatively through  propagation by using mini-cuttings with the use of Indole Butyric Acids (IBA). A study was conducted to compare rooting success of mini-cuttings of herbaceous stem of four guava cultivars with IBA treatment. Four guava cultivars tested were Bengkulu Round, Getas, Crystal, and Bangkok.  Mini-cuttings were immersed in IBA solution at 0 ppm or 1000 ppm. Cultivar and IBA treatments were arranged factorially in a randomized complete  block design with  3 replications, each consisting of 25 cuttings. Variables observed included percentage of original-leaf shedding, percentage of emerging shoots, percentage of sprouting cuttings, new leaf number, root number, root length, and percent of rooted cuttings. The results demonstrated that cultivars affected rooting percentage, sprouted percentage, percentage of original-leaf fall, and number of root.  Getas had highest rooting and sprouting percentages, and showed lowest number of  original-leaf shedding; whereas, Bangkok demostrated greatest number of root.  IBA treatment increased the number of root and the number of original-leaf shedding, but reducing rooting percentage of mini-cutting. There was no interaction between cultivar and IBA teratment.
Growth and Yield Response of Detam 4 Soybean Variety on Single P Fertilizer and Vermicompost Application in Coastal Land Cahyadi, Robi; Pujiwati, Hesti; Murcitro, Bambang Gonggo; Suprijono, Eko; Prameswari, Wuri
TERRA : Journal of Land Restoration Vol. 7 No. 1 (2024)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/terra.7.1.33-41

Abstract

This research was conducted from July to November 2020 in the coastal lands of Ratu Agung District, Lempuing Village, Kuala Alam, Bengkulu City, at an elevation of 5 meters above sea level. The study aimed to investigate the interaction between vermicompost and single phosphorus (P) fertilizer on the growth and yield of Detam 4 soybean plants in coastal lands, focusing on determining the optimal doses for both inputs. The experiment was designed as a two-factor study using a factorial Randomized Complete Block Design (RCBD) with three replications. The first factor was vermicompost application at four levels: 0 tons ha-1, 5 tons ha-1, 10 tons ha-1, and 15 tons ha-1. The second factor was phosphorus application at three levels: 0 kg ha-1, 50 kg ha-1, and 100 kg ha-1. Observed variables included plant height, number of leaves, flowering age, number of productive branches, number of pods per plant, pod weight per plant, pod weight per plot, seed weight, and root weight. Data were analyzed using ANOVA at the 5% significance level, with a subsequent 5% LSD test if significant effects were found. The application of vermicompost at a dose of 10 tons ha-1 without the addition of single P fertilizer results in the best growth of the soybean variety Detam 4, characterized by the highest average shoot dry weight of 41.133 g, the fastest flowering age, and the greatest number of productive branches. The best yield of the Detam 4 soybean variety is achieved with the application of vermicompost at a dose of 15 tons ha-1, indicated by the highest number of productive branches and seed weight per plant. The independent application of single P fertilizer does not significantly enhance the growth and yield of the Detam 4 soybean variety in coastal land conditions.  
Co-Authors Atra Romeida Bambang Gonggo Murcitro Gustian, Meko Hairani, Putri Mian Herison, Catur HESTI PUJIwATI Hutabarat, Grace May Sawita Marwanto Merakati Handajaningsih Mohammad Chozin, Mohammad Nafisah, Dio Rahayu, Hatari Rahayu, Hatari Robi Cahyadi, Robi Rustikawati Rustikawati Sari, Dia Novita Septiana Anggraini, Septiana Simanihuruk, Bilman Wilman Sumardi . Supanjani, Supanjani Supanjani, Supanjani Surjono H. Sutjahjo Utami, Kartika Vadillah, Syavirah Viani, Reza Junita Okta Widodo Widodo Widodo Wuri Prameswari Yulian, Yulian Yulian, Yulian Zainal Muktamar Zikri, Ahmad