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All Journal Jurnal Ilmu Pertanian Indonesia VALENSI agriTECH KOVALEN: Jurnal Riset Kimia Science and Technology Indonesia Indonesian Journal of Chemistry Jurnal Perikanan Universitas Gadjah Mada Indonesian Journal of Applied Research (IJAR) Jurnal Pijar MIPA Makara Journal of Science Journal of Pharmaceutical and Sciences. Halal Studies and Society
Noviyan Darmawan
Department Of Chemistry, Bogor Agricultural University, Jl. Agatis, Kampus IPB Darmaga, Bogor 16680
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Journal : Science and Technology Indonesia

 1 
Carbon Dots-based Antifungal Coating Film Against Pathogens Colletotrichum sp. for Active Coating Application of Mango Safitri, Idayu; Sugiarti, Sri; Darmawan, Noviyan
Science and Technology Indonesia Vol. 9 No. 1 (2024): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.1.173-182

Abstract

Mangos are one of horticultural products that are leading the Indonesian agricultural industry. Simple post-harvest handling leads to high damage. The most common causes is anthracnose disease caused by Colletotrichum sp. Therefore, it’s necessary to modify the existing methods in postharvest handling such as coating. Adding active agents such as carbon dot (CD) in coating material to prevent anthracnose from growing and shorten the shelf life. This study aims to investigate the applicability and antifungal properties of CD as a composite coating. CDs were synthesized using chitosan as the carbon source in a one-pot hydrothermal technique. The CDs have blue-emitting luminescence caused by the functional group at the surface of CD. The CDs were used to prepare chitosan/pectin (Chi/Pec)-based composite coatings. The addition of CDs increased the viscosity and density of composites, increased the blue-emitting luminescence, and showed potential antioxidant activity. The CDs and composite coatings exhibited high antifungal activity against Colletotrichum sp. by agar well diffusion method and were classified as very strong antifungal agents. The chitosan/pectin/CDs-coating effectively reduced the growth of black spots on the surface of mangos and increased the fruit shelf life of the fruit by 24 days. The CDs are evidently safe, affordable, and value-added nanomaterials that can be used to prepare active packaging applications.
Assessment of Commercial DNA Extraction Kits for Porcine Gelatin Detection Using RT-PCR and ddPCR Gina, Safira; Rahmagiarti, Cintera; Ummah, Isna Mustafiatul; Sumantri, Cece; Suparto, Irma Herawati; Darmawan, Noviyan
Science and Technology Indonesia Vol. 9 No. 3 (2024): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.3.605-612

Abstract

Gelatin is extensively applied in various industries, including food, beverages, cosmetics, and pharmaceuticals. Although the determination of gelatin species is essential for religious, health, and consumer preference reasons, a standardized analysis method is absent. The challenge in identifying gelatin through DNA-based methods arises from the low DNA content and extensive DNA denaturation in the gelatin matrix. This study assessed the efficacy of two commercial DNA extraction kits, namely the Processed Food DNA Extraction (PF kit) and the DNeasy Mericon Food Kit (DM kit), for extracting DNA from porcine gelatin powder and commercial products derived from gelatin. Additionally, we evaluate the amplification of the extracted porcine DNA using real time polymerase chain reaction (RT-PCR) and droplet digital polymerase chain reaction (ddPCR) techniques. The PF extraction kit demonstrated successful DNA extraction from porcine gelatin powder and commercial samples of porcine gelatin-based candies with a higher concentration (32.24-286.07 ng/uL) and purity (A260/A280 ratio of 1.82-2.33) compared to the DM kit (3.95-7.30 ng/uL and an A260/A280 ratio of 1.29-2.45). RT-PCR and ddPCR analyses yielded positive results for porcine DNA from gelatin powder for both PF and DM kits, albeit with differing Cq values and copy numbers. The choice of DNA extraction kit significantly impacted the amplification results when analyzing commercial samples of porcine gelatin-based candies. Using RT-PCR, all samples yielded negative results with the DM kit, while the PF kit detected one positive result for porcine DNA. Improved outcomes were observed with more sensitive analysis methods such as ddPCR, where the DM kit identified one positive result for porcine DNA while the PF kit detected positive results for all tested candies.
Fluorine Substitution in Diamine Covalent Organic Frameworks: Computational Analysis of CO2/N2 Adsorption and Permeability Darmawan, Noviyan; Apriliyanto, Yusuf Bramastya; Jati, Andreas Ary Chrisna; Kusumawardani, Cahyorini
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.18-26

Abstract

In this study, we investigated the effect of fluorine substitution on a previously reported diamine based covalent organic framework (COF), designated as IPB-2H. A new fluorinated analogue namely IPB-2F2 was modeled and its adsorption and permeability characteristics for CO2/N2 gas mixtures were evaluated through computational analysis. Ab initio structural optimization results showed that the reduced pore size of IPB-2F2 compared to IPB-2H was attributed to the larger atomic size and higher electronegativity of fluorine compared to hydrogen atom. Molecular dynamics (MD) simulations demonstrated that IPB-2F2 exhibited lower permeation rates for CO2 and N2 than its non fluorinated counterpart; indicating that fluorine atoms effectively reduced gas permeation. Adsorption isotherms revealed enhanced adsorption capacities for IPB-2F2, with increased CO2 affinity resulting from strong van der Waals interactions. Selectivity analyses showed that IPB-2F2 preferentially absorbed CO2 over N2, with selectivity values consistently greater than 1. The enhanced gas uptake capacity and hydrophobicity of IPB-2F2 highlighted its potential for industrial applications as a post-combustion CO2 capture material.
Co-Authors Ali, Mohamad Makrus Ani Suryani Suryani Arrahman, Naufal Bella Fitri Rahmantika Cahyorini Kusumawardani Cece Sumantri Dedri Syafei Dedy Duryadi Solihin Dias Indrasti DIMAS ANDRIANTO Dini, Vianda Mayeri Dwiatmoko, Adid Adep Dwiyani, Dewa Ayu Made Safira Fadila, Rina Fitriana Faradisa Anindita Fendy, Fendy Firdaus, Dzikri Anfasa Gina, Safira Ida Ayu Putu Sri Widnyani Indriani, Mutiara Rachmina Irma Herawati Suparto Isalmi Aziz Jati, Andreas Ary Chrisna Khairani, Najwa Azka Lilis Sulistiawaty Lubis, Rifaldy Setiawan M.Saad, Nabilatul Rafi’ah Mala Nurilmala Mas’ud, Zainal Alim Mohammad Khotib Mubayyinah, Hamidatul Muh Tazri Nasirullah Muhammad Faqih Mukhlisin Mukhlisin, Muhammad Faqih Murheza, Kevin Nancy Dewi Yuliana Pranata, Agy Wirabudi Purwantiningsih Sugita Rahmagiarti, Cintera Rudhatin, Fayca Safitri, Idayu Sanneh, Kally Sari , Intan Dias Sitompul, Dina Wardani Sri Sugiarti Supratikno Suryawan, Chinesia Prastialin TATI NURHAYATI Tetty Kemala Tun Tedja Irawadi Ummah, Isna Mustafiatul Yuli Rahmawati Yuni Cahya Endrawati Yusuf Bramastya Apriliyanto Zainal Alim Mas'ud