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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Food and Pharmaceutical Science Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Tropical and Infectious Disease Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY The Indonesian Biomedical Journal JFIOnline Journal of Food and Pharmaceutical Sciences
Ratna Asmah Susidarti
Faculty Of Pharmacy, Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cells Meiyanto, Edy; Susidarti, Ratna Asmah; Handayani, Sri; Rahmi, Fitria
INDONESIAN JOURNAL OF PHARMACY Vol 19 No 1, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.642 KB) | DOI: 10.14499/indonesianjpharm0iss0pp12-19

Abstract

Areca catechu seed contains antioxydant substances, supposed to have anticancer property. This research therefore addressed to examine the inhibitory effect of Areca catechu seed ethanolic extract (EP) on proliferating breast cancer cells, MCF-7. Areca catechu seed ethanolic extract (EP) standardization was done according to the standard of BPOM. Areca catechu seed powder extraction was done using ethanol 96%. Cytotoxic assay – to get the value of IC50 and to prevent the cell proliferation (using doubling time assay) – was carried out by using MTT assay. Apoptosis observation was done by acrydine orange- etidium bromide staining method (double staining). The result showed that treatment with Areca catechu seed ethanolic extract (25-100 µg/m) for 48 h caused 13-84% growth inhibition (IC 5077 µg/mL) of the cells, while arecoline (ARE) treatment (10-500 µg/mL) showed 8-73% inhibition (IC50 180 µg/mL). The extract also inhibited cell proliferation and induced apoptosis. These results conclude that Areca catechu seed ethanolic extract (EP) possesses antiproliferative effect through growth inhibition and apoptosis induction.Key words:MCF-7, Areca catechu, antiproliferative 
SUN PROTECTOR FACTOR (SPF) IN VITRO AND THE PHYSICAL STABILITY OF O/W CREAM OPTIMAL FORMULA FROM THE PARTITION PRODUCT OF MAHKOTA DEWA LEAVES [Phaleria macrocarpa (Scheff) Boerl] Zulkarnain, Abdul Karim; Marchaban, Marchaban; Wahyuono, Subagus; Susidarti, Ratna Asmah
Indonesian Journal of Pharmacy Vol 26 No 4, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1137.832 KB) | DOI: 10.14499/indonesianjpharm26iss4pp210

Abstract

The leaves Mahkota dewa (Phaleria macrocarpa (Scheff) Boerl) contains a benzophenoic glycoside (Phalerin) which is active for sun screen. Some formula and designed on the Simplex Lattice Design (SLD) method then its physical characteristics were investigated. The physical characteristics will be used to determine the optimum formula. In present study, the optimum formulation was evaluated to determine chemical stability and the in vitro SPF. The doses of partition product of P. macrocarpa was used 6%. The optimal formulation cream was determined by SLD version 9.0.1 with variety of Cetyl alcohol, mineral oil, and Tween 80. The difference of physical characteristics between experimental and software was analyzed using T-test, P= 95 %. The physical stability and in vitro SPF of the optimum formula was observed. he study exhibits that the combination of cetyl alchodol 9,71%, mineral oil 29%, and tween 80 3,29% was the optimum formula of o/w cream with the desirability test 0,924. The T-test result test shows that there is no significant difference (p>0.05) on physical characteristics between experimental and software prediction. During the storage, the viscosity, spread ability, adhesive ability, separation volume ratio of cream was relatively stable however the stability started to decline on the week IV in the extreme temperature. The SPF of the cream of the CFR, CPHR and CBZP partition were 21,32; 33,12 and 42,49, respectively.Keywords: Mahkota dewa, cream, optimum, SPF
THE EFFECT OF GIVING DELTAMETHRIN AND CYPERMETHRIN INSECTICIDES ON THE LEVEL OF INTRATESTICULAR TESTOSTERONE IN MALE WISTAR STRAIN RATS Triutomo, Devyanto Hadi; Puspitasari, Ika; Susidarti, Ratna Asmah
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp116

Abstract

The use of deltamethrin and cypermethrin insecticides to control pests has been implemented in Indonesia. Irregular and excessive use of insecticides can have toxic effects on the male reproduction system. Deltamethrin and cypermethrin insecticides can inhibit androgen biosynthesis and disrupt the growth of sperm. Therefore, this research is conducted to find out the effect of deltamethrin and cypermethrin insecticides toward the level of intratesticular testosterone in male Wistar strain rats. This research is conducted in vivo using male Wistar strain rats. Nine rats are divided into three groups of treatment, namely control group, deltamethrin group with 0.26 mg/kg of weight, and cypermethrin group with 0.26 mg/kg of weight. Treatment is conducted every day for 15 days orally, and then the rats are dissected to take their testicles. Testicles are chopped and their intratesticular homogenates are taken by adding the medium of DMEM and collagenase (0.25 mg/mL). The measurement of the level of testosterone is conducted by using Electrochemiluminescence immunoassay (ECLIA) method. Data on the change of the increase of body weight, organ weight, and the level of testosterone is analyzed statistically using the one-way ANOVA test. The research result shows that giving 0.26 mg/kg of weight of deltamethrin and 0.26 mg/kg of weight of cypermethrin does not affect the increase in body weight, relative weight of the reproductive organ, and the level of intratesticular testosterone of male Wistar strain rats.
INCREASING SENSITIVITY OF MCF-7/DOX CELLS TOWARDS DOXORUBICIN BY HESPERETIN THROUGH SUPPRESSION OF P-GLYCOPROTEIN EXPRESSION Sarmoko, .; Pamungkas P, Dyaningtyas Dewi; Susidarti, Ratna Asmah; Nugroho, Agung Endro; Meiyanto, Edy
Indonesian Journal of Pharmacy Vol 25 No 2, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (870.371 KB) | DOI: 10.14499/indonesianjpharm25iss2pp84

Abstract

Long-term use of doxorubicin causes cancer resistance due to overexpression of P-glycoprotein (P-gp), a protein that plays a role in cell drug efflux. The purpose of this study is to determine the action of hesperetin in increasing the cytotoxicity of doxorubicin on MCF-7 cancer cells resistant to doxorubicin (MCF-7/DOX) through suppression of P-gp expression. Cytotoxic assay of single and combinational treatment of doxorubicin and hesperetin were performed by using MTT assay. Apoptosis evidence was examined by using double staining with acridine orange and ethidium bromide dyes, while Pgp expression was determined by using immunocytochemistry. Hesperetin reduced cell viability in dose dependent manner. Both MCF-7 ori and MCF-7/DOX cells gave different responses to hesperetin with the IC50 values of >500μM and 267μM, respectively. Combining treatment of hesperetin and doxorubicin to MCF-7/DOX cells at the dose of 95μM and 230nM increased apoptosis evidence and suppressed P-gp expression. These results suggest that hesperetin enhances the anticancer effect of doxorubicin to resistance MCF-7 cells through suppression of P-gp expression.
Hepatoprotective Activity of Ethyl Acetate Fraction of Senggugu’s Root Bark (Clerodendrum serratum L.Moon) on Rats Induced by CCl4 Nasrudin, Nasrudin; Wahyono, Wahyono; Mustofa, Mustofa; Asmah, Ratna
Indonesian Journal of Pharmacy Vol 28 No 1, 2017
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (748.622 KB) | DOI: 10.14499/indonesianjpharm28iss1pp10

Abstract

Senggugu is a plant that has long been used to treat syphilis, typhoid, cancer, jaundice, and hypertension. The pharmacological activity of senggugu in Indonesia that have been reported include antifertility activity in leaves, mucolytic activity, anti-inflammatory and tracheospasmolytic, also antioxidant in its root bark. This study aims to determine the hepatoprotective effect of ethyl acetate extract fraction of senggugu’s root bark in rats induced by CCl4. The powder of senggugu’s root bark was extracted by terraced maceration method starts from n-hexane, ethyl acetate, to methanol, thus obtained ethyl acetate extract fraction of senggugu’s root bark (FEAKAS). The ethyl acetate extract fractions were then tested for hepatoproctective activity using doses of 25, 50, and 100 mg/Kg.BW on rats induced by CCl4. FEAKAS hepatoprotective activity was determined from the analysis of blood biochemical and oxidative stress parameters. The blood biochemical parameters included SGOT (serum glutamic oxaloacetic transaminase), SGPT (serum glutamic pyruvic transaminase), ALP (alkaline phosphatase), bilirubin, and total protein were measured with test kit. The oxidative stress parameters were measured from homogenates of liver tissue that were prepared by adding 500 mL of 50 nM Tris buffer (pH 7.4) containing 1 mM EDTA and 10 µg/mL leupeptin. The homogenates were centrifuged to obtain supernatants for measurement of oxidative stress parameters using spectrophotometer method, including MDA (malondialdehyde), GPx (glutathione peroxidase) and CAT (catalase). The results showed that the effect of FEAKAS against CCl4 induction for preventing lipid peroxidation, from both blood chemical and oxidative stress parameters, are shown at a dose of 100 mg/Kg.BW that significantly different compared to CCl4 control (ρ <0.05) on all blood chemical and oxidative stress parameters.
Hesperidin increase cytotoxic effect of doxorubicin in MCF-7 cells Hermawan, Adam; Meiyanto, Edy; Susidarti, Ratna Asmah
Indonesian Journal of Pharmacy Vol 21 No 1, 2010
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.522 KB) | DOI: 10.14499/indonesianjpharm0iss0pp8-17

Abstract

Hesperidin,  a  flavonoid,  shows  strong  cytotoxic  effect  in  several  cancer cell  lines.  The  aim  of  this  research  was  to  investigate  cytotoxic  activities  of hesperidin  alone  and  in  combination  with  doxorubicin.  Cell  viability  assay  of hesperidin,  doxorubicin,  and  combination  treatments  were  carried  out  by  using MTT  assay.  Apoptosis  assay  was  done  using  double  staining  method  using Ethidium  Bromide-Acridine  Orange.  Hesperidin  did  not  show  cytotoxic  effect but doxorubicin showed cytotoxic effect with IC50467 nM. Hesperidin (5, 50 and 100  µM)  increased  cytotoxic  effect  of  doxorubicin  compared  with  doxorubicin alone.  The  strongest  cytotoxic  activity  was  showed  by  the  combination  of  200 nM  doxorubicin  and  100  µM  hesperidin.  Combination  treatment  of  doxorubicin 200  nM  and  hesperidin  100  µM  induced  apoptosis  in  MCF-7 cells.  Hesperidin  is potentially  to  be  developed  as  co-chemotherapeutic  agent  for  breast  cancer, while molecular mechanism need to be explored.Key words: Hesperidin, doxorubicin, synergism, MCF-7, apoptosis 
Visible spectrophotometric determination of Cefadroxil using ethyl acetoacetate and formaldehyde reagents Susidarti, Ratna Asmah; Rianti, Andrih; Martono, Sudibyo
Indonesian Journal of Pharmacy Vol 19 No 1, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.341 KB) | DOI: 10.14499/indonesianjpharm0iss0pp41-47

Abstract

Visible spectrophotometric method can be used to determine cefadroxil in the pharmaceutical dosage form. The reaction of cefadroxil with the condensation product of 2 mol ethyl acetoacetate and 1 mol formaldehyde in acidic condition (pH 3,5) at 45°C for 20 minutes yielded yellow product giving absorption at 367 nm. The concentration of Cefadroxil was calculated by using standard curve Y = 1.6063 X + 0.1634, r = 0.9932, p = 0,001 and Vxo = 9,7%. Determination of cefadroxil capsule ”X” (562.4 mg/capsule) showed that the concentration of cefadroxil observed is 490.5 mg/capsule (CV = 0.89%) and recovery value is 102.88% (CV = 0.67%). Key words: visible spectrophotometry, Cefadroxil, ethyl acetoacetate, formaldehyde
Antioxidant–free radical scavenging of flavonoid from The Leaves of Stelechocarpus burahol (Bl.) Hook f. & Th. Sunarni, Titik; Pramono, Suwidjiyo; Asmah, Ratna
Indonesian Journal of Pharmacy Vol 18 No 3, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.139 KB) | DOI: 10.14499/indonesianjpharm0iss0pp111-116

Abstract

The activity testing of flavonoid compounds as antioxidant and as scavenger of free radical, isolated from the Stelechocarpus burahol leaves had been performed. The aqueous extract of the leaves was concentrated and then suspended in ethanol to produce ethanolic fraction. The fraction was chromatographed on several paper chromatography systems to produce isolate with chromatographic purity.The all isolated flavonoid was identified by paper chromatography system. Especially, the B4b isolate were identified further using spectrometer UV-Vis, FT-IR and ¹H-NMR. Their antioxidant activities were done by the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method.The all isolated flavonoid showed activity as DPPH scavenger. Among these isolate, B4b exhibited a strong free radical scavenging with an EC50 value of 6.43 μg/mL. They were identified and B4b isolate was pressumed as flavon with hydroxyl group on C-3, C-7, C-3', C-4' and methyl on C-5.Key words : Stelechocarpus burahol, flavonoid, antioxidant, DPPH
Co-Authors . Larasati . Sismindari . Warsinah Abdul Karim Zulkarnain Abdul Manaf Ali, Abdul Manaf Abdul Rohman Adam Hermawan Aditya Fitriasari Agung Endro Nugroho Agustina Setiawati Agustina Styawan, Anita Alfi Yasmina Aulia Katarina Edy Meiyanto Endah Puji Septisetyani, Endah Puji Fany Mutia Cahyani Fitria Rahmi Harsan, Hayfa Salsabila Herwandhani Putri Ika Puspitasari Indah Purwantini Indri Kusharyanti Inna Armandari Kartika Dyah Palupi Kholid Alfan Nur Marchaban, Marchaban Martono, Sudibyo Marvin Lambertus Meiyanto, Edy Meiyanto, Edy Mintarsih, Betty Mintarsih, Betty Muflikhasari, Haruma Anggraini Mustofa Mustofa Mustofa, Mustofa Muthi Ikawati Nasrudin, Nasrudin Octa Ujiantari, Navista Sri Pamungkas P, Dyaningtyas Dewi Pramono, Suwidjiyo Purwanto Rahmah, Inggita Hasi Rahman, Faaza Aulia Rahmani, Mawardi Rahmani, Mawardi Rahmawati, Desty Restia Rahmi Khamsita Raisatun Nisa Sugiyanto Rianti, Andrih Riris Istighfari Jenie Rohmad Yudi Utomo Sarmoko Sarmoko Sendy Junedi Sofa Farida Sri Handayani Sukari, Mohd. Aspollah Sukari, Mohd. Aspollah Sunarni, Titik Triutomo, Devyanto Hadi Wahyono Wahyono Wahyono, Wahyono Wahyuono, Subagus Wibowo, Andy Eko Windarsih, Anjar Zufairo, Shofa Khamdanatuz