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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science Jurnal Biologi Universitas Andalas BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Buletin Penelitian Tanaman Rempah dan Obat Agroscript: Journal of Applied Agricultural Sciences
Sitti Fatimah Syahid
Balai Penelitian Tanaman Obat dan Aromatik
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Immunology & microbiology Industrial & Manufacturing Engineering Veterinary

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In Vitro Conservation of Medicinal Plant St. John’s Wort (Hypericum perforatum L.) Through Dilution of Basic Medium Syahid, Sitti Fatimah
AGROSCRIPT: Journal of Applied Agricultural Sciences Vol 3 No 1 (2021): June
Publisher : Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/agroscript.v3i1.589

Abstract

In vitro techniques could be applied for plant conservation through minimal growth. Growth reduction is generally attained by modifying the culture medium and/or the environmental condition. Conservation of St. John’s wort (Hypericum perforatum L.) in vitro through dilution of basic medium was conducted at the Tissue Culture Laboratory of Indonesian Spice and Medicinal Crops Research Institute (ISMCRI) in 2018. Sterile shoots were cultured into Murashige and Skoog (MS) medium by reducing macronutrients. The treatments used were full-MS + 0.1 mg L-1 N6 - benzyladenine (BA) as control; ¾ MS + 0.1 mg L-1 BA; ½ MS + 0.1 mgL-1 BA and ¼ MS + 0.1 mg L-1 BA. Observation of the culture growth was conducted three months after the treatments. The experiment was arranged in Completely Randomized Design with ten replications. The result showed that the use of a dilution of basic medium affected the growth of St. John’s wort. The use of ¼ MS + 0.1 mg L-1 BA composition could suppress culture growth (number of shoots, shoots length and number of leaves), without showing necrotic symptom until three months of conservation. This treatment could be used as an alternative to minimize the culture of St. John’s wort for in vitro conservation.
SOMATIC EMBRYOGENESIS FROM MERISTEM EXPLANTS OF GINGER Rostiana, Otih; Syahid, Sitti Fatimah
BIOTROPIA Vol. 15 No. 1 (2008): BIOTROPIA Vol. 15 No. 1 June 2008
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (574.099 KB) | DOI: 10.11598/btb.2008.15.1.2

Abstract

The use of planting materials from in vitro culture, especially derived from somatic embryos has some advantages such as  genetically stable and pathogen-free. Meristem culture of ginger through somatic embryogenesis could be a potential method for producing pathogen-free planting materials. Somatic embryogenesis on ginger was performed to obtain vigorous plantlets having the same rhizome size as the mother plant. Callus was induced from meristem tissue of inner bud of Indonesian ginger rhizome Var. Cimanggu-1 and consecutively subcultured into certain media at each steps of experiments. The vigorous embryogenic calli were observed  on MS medium containing 100 mgl-1 glutamine and 2% sucrose with addition of 1.0 mgl-1 2,4-D + 3.0 mgl-1 BA. The highest number of somatic embryos (about 82.0.g-1 friable calli) was achieved on that medium, 4 weeks after culturing. Furthermore, the optimum growth of embryogenic calli containing somatic embryo was obtained on MS medium enriched with 6% sucrose. The highest number of mature somatic embryos (57.2 embryos) was achieved on MS medium, 18 days after incubation. The regeneration potency of somatic embryos obtained from ginger meristem was 51.20%.g-1 friable callus. The valuable result of this study was the achievement of normal rhizome size of regenerated plantlets, instead of micro rhizome.
Co-Authors Budi Martono Desriani Desriani Endang Hadipoentyanti Hadipoentyanti, Endang Hapsari, Betalini Widhi Haz, Aufa Rizqia Kumala, Ajeng Putri MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH Natalini Nova Kristina Noorohmah, Siti NUR AJIJAH, NUR Nurliani Bermawie Otih Rostiana Rahayu, Resa Sri Raihan, Eldrian Daffa Rantau, Deritha Ellfy Seti, Lusia SUSI PURWIYANTI, SUSI Tias Arlianti Wulandari, Dyah Retno Yuliawati