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PENGARUH EKSTRAK KULIT BUAH MANGGIS (Garcinia mangostana L.) TERHADAP SIKLUS ESTRUS MENCIT (Mus musculus L. Swiss Webster) Maria Gorette, Julia; Sumarmin, Ramadhan; Widiana, Rina
Pendidikan Biologi Vol 1, No 1 (2014)
Publisher : STKIP PGRI Sumbar

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Abstract

 ABSTRACTMangosteen (Garcinia mangostana L.) is a tropical plant that originated from Southeast Asia. Mangosteenrindis used to treat various diseases, antihistamine, antibacterial, anti-cancer, anti-hypertension and stroke. This study aims to determine the effect of extracts of mangosteen rind (Garcinia mangostana L.) on the estrous cycle of mice (Mus musculus L. Swiss Webster). This study is an experiment used Completely Randomized Design ( CRD ), 4 treatments and 6 replications. Mangosteen peel extract given to mice by gavage for 24 days way. The extract was given to mice at a dose of B 0.2, C 0.4, D 0.6 and Control A 0 g /head/day. Observations made during the estrous cycle of 26 days to review the vaginal smear method with stained Giemsa. The data obtained were analyzed by Analysis of Variance by analyzing the average length of one cycle and each phase of the estrous cycle of mice and Duncan New Multiple Range Test (DNMRT).The results showed mangosteen peel extract can prolong the estrous cycle during the (± 3 days) and extension occurred in all phases of the cycle (proestrus, estrous, and diestrus, metestrus ). Added time proestrus phase of the shortest found in A (1.25 days) and longest in treatment C (2 days). Estrous phase of the shortest found in A (2.13 days) and longest in treatment D (3 days). Metestrus  phase the shortest found in A (1.71 days) and longest in treatment D (3.58 days). Diestrus phase of the shortest found in A ( 1.42 days ) and longest in treatment D ( 3.52 days ). Added estrous cycle at a time (6.5 days), B (9.33 days), C (10.07 days) and D (11.53 days). From these results it can be concluded that mangosteen peel extract given orally prolong the estrous cycle and extension at each phase of the estrous cycle
KONSENTRASI HAMBAT MINIMUM (KHM) EKSTRAK DAUN TEH (Camellia sinensis L.) PADA Escherichia coli DAN Salmonella sp. WIDIANA S.Si, M.Si, RINA
Jurnal Pelangi : Research of Education and Development Vol 4, No 2 (2012)
Publisher : STKIP PGRI Sumatera Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.713 KB) | DOI: 10.22202/jp.2012.v4i2.14

Abstract

Diarrhea is the condition loosing too much liquid and electrolyte throughfeces and it is second biggest cause of infant death in Indonesia. Diarrhea iscaused by Salmonella sp and Escherichia coli bacteria. Inappropriate drug usedsuch as anti diarrhea and antibiotic can cause another health problem. Analternative treatment which does not cause side effect to the diarrhea victim isusing an anti microbe active of the plant medicine. One of the plant which is usedby the people as a treatment for the diarrhea victim is tea leaves (Camelliasinensis L.). it contains anti microbe active such as catecin, tannin, flavonoid andsaponin. Thus, the research was conducted to find out the minimum restrictionconcentrate of Camellia sinensis L. in E. coli and Salmonella sp.The minimum restriction concentration is used dilution method with jellyas the media (Jelly dilution method) by double it. The concentrations of E. coliare 50 %, 25 %, 12,5 %, 6,25 %, 3,125 % and 1,56 % with 10 % antibioticampicilin control. The concentrations for Salmonella sp are 50 %, 25 %, 12,5 %,6,25 %, 3,125 %, 1,56 %, 0,78 %, 0,39 %, 0,195 % and 0,0975 %. The data wasanalyzed in qualitative method by examining the minimum restriction of tealeaves extract (Camellia sinensis L.) in the bacteria that caused diarrhea. Theresearch was conducted on August 2010 in Biology Laboratory of STKIP PGRIWest Sumatera. Tea leaves was extracted in Chemical Laboratory of KopertisWilayah X Padang. The research found that E. coli is more resistant to tea leavesthan Salmonella sp. The minimum restriction concentrate of tea leaves extract(Camellia sinensis L.) in E. coli is 3,125% and 0,0975% in Salmonella sp.
KOMPOSISI FITOPLANKTON YANG TERDAPAT DI PERAIRAN BATANG PALANGKI KABUPATEN SIJUNJUNG Widiana, S.Si., M.Si, Rina
Jurnal Pelangi : Research of Education and Development Vol 5, No 1 (2012)
Publisher : STKIP PGRI Sumatera Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.43 KB) | DOI: 10.22202/jp.2013.v5i1.4

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ABSTRACTPhytoplankton is water plant which floats freely and float away in water andable to photosynthesis. Phytoplankton has important role since it become foodfor animals are life in that water, such as fish and insect. Morever, phytoplanktonis able to influence the quality of water. There are some houses, livestock marketand farmland. Water of Batang Palangki river is used to fulfill daily needs, meansof gold mining, sand, bathing place for livestock and fish catching. A researchhas been done in Batang Palangki river. Its purpose is to know the phytoplanktoncomposition in Batang Palangki river. This research was conducted by usingdescriptive survey method which chose four research stations that is station Isettlement area, station II farm, station III gold mining area and station IV postarea of gold mining. Sample was selected in 3 spots for each station, namely left,right and center of river. Parameter which was measured was abundance, relativedensity, frequency and relative frequency, and quality parameter of water whichwas measured was temperature, pH, the flow of speed, DO, and CO2. From theresult of this research, it was found that the phyotoplankton composition was 4classes, 4 ordo, 9 families and 13 genus. Total abundance was 24,68 individu/l,abundance is highest in station II was 8,34 individu/l, and then station I was 7,98individu/l, station IV was 4,93 individu/l and station III was 3,43 individu/l. Classthat highest abundance was Bacillariophyceae, was 16,55 individu/l and minimallow was Euglenophyceae, was 0,46 individu/l. Genus that highest abundance wasNavicula, was 4,78 individu/l and minimal low was Phacus was 0,46 individu/l.Key word: water pollution, composition, phytoplankton
EFEK TOKSIT DAN TERATOGENIK EKSTRAK BROTOWALI (Tinospora crispa L.) TERHADAP SISTEM REPRODUKSI DAN EMBRIO MENCIT (Mus musculus L. Swiss Webster) widiana, rina; Sumarmin, Ramadhan
Jurnal Bioconcetta Vol 2, No 1 (2016)
Publisher : STKIP PGRI Sumatera Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.292 KB) | DOI: 10.22202/bc.2016.v2i1.1294

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ditemukan di semua bagian tanaman ini. Batang Tinospora crispa mengandung kolumbin,  zat  pahit,  alkaloid  dan glicosida.  Alkaloid  merupakan zat antiproliferatif, dimana zat antiproliferatif ini dapat mengurangi jumlah oosit yang dihasilkan dan mengurangi jumlah oosit yang mengalami ovulasi sehingga mempengaruhi penampilan reproduksi mencit. Penelitian ini bertujuan untuk mengetahui efek  toksik dan teratogenik  ekstrak  Tinospora crispa pada sistem reproduksi dan embrio tikus. Penelitian ini merupakan penelitian eksperimen dengan menggunakan mencit betina (Mus musculus L.) sebanyak 24 ekor. Penelitian memakai rancangan acak lengkap dengan empat perlakuan dan enam ulangan.  Perlakuan  yang  diberikan  adalah  ekstrak  brotowali  dengan  berbagaidosis, yaitu: A adalah kontrol, perlakuan B (5x10-2), C (6x10-2) dan D (7x10-2)g/mencit.  Parameter  yang  diamati  adalah  jumlah  korpus  luteum,  implantasi, embrio  resorpsi,  janin  mati dan  janin  hidup.  Data  dianalisis  dengan  Analisis Varians dan uji lanjut DNMRT pada taraf α 5%. Hasil penelitian menunjukkan bahwa ekstrak brotowali berpengaruh nyata terhadap jumlah korpus luteum, janin hidup, janin mati dan embrio resorpsi mencit dengan 0 sampai 16 hari kehamilan. Sementara, terhadap jumlah implantasi berpengaruh tidak nyata, sehingga dapat disimpulkan bahwa ekstrak brotowali memiliki efek embriotoksik dan fetotoksik terhadap mencit betina hamil.
DAYA HAMBAT EKSTRAK DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP PERTUMBUHAN BAKTERI PENYEBAB DIARE Widiana, Rina; Indriati, Gustina; Harsinta, Nora
Sainstek : Jurnal Sains dan Teknologi Vol 3, No 1 (2011)
Publisher : IAIN Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (513.704 KB) | DOI: 10.31958/js.v3i1.34

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Diarrhea is the second biggest cause of infant death in Indonesia.  Diarrhea can caused by several factors, one of them is bacteria.  The most common bacteria which cause diarrhea are Escherichia coli and Salmonella sp.  The common treatment for the diarrhea victim uses synthetic antibiotic.  Unfortunately, it gives side effect such as; fever, allergic, headache and resistance to strain microbe.  Therefore the alternative treatment which does not cause side effect to the diarrhea victim is using antibiotic from plant need to be found immediately.  One of them is using the extract from leave of morinda (Morinda citrifolia L.). Thus this research had been conducted in order to find out morinda leave extract in restricting  the growth of E. coli and Salmonella sp.  This research was conducted on Desember 2010 in Biology Laboratory of Biology department of STKIP PGRI West Sumatera. Experiment design is randomized completely design with six treatment and four replication.  Treatment A used Amoxicilin and treatment B used morinda leave extract with level of concentration15 %, C 20 %, D 25 %, E 30 % and F 35 %. The method paper diffusion used to determine the diameter of inhibition area of morinda leave extract. The data was analyzed with ANOVA and continued with least significant different test with p<0.05.  The result showed that mengkudu leave extract can decrease the growth of E. coli and Salmonella sp bacteria where Salmonella sp is more sensitive than E. coli.Key words: diarrhea, escherichia  coli, salmonella sp, morinda leave extract, amoxicilin
KOMPOSISI ARTHROPODA PERMUKAAN TANAH DI KAWASAN PENAMBANGAN BATUBARA DI KECAMATAN TALAWI SAWAHLUNTO Nurhadi, Nurhadi; widiana, Rina
Sainstek : Jurnal Sains dan Teknologi Vol 2, No 1 (2010)
Publisher : IAIN Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (733.157 KB) | DOI: 10.31958/js.v2i1.7

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The research has been done to test the arthropod composition of surface soil at coal mining territory in Talawi district. The arthropod samples were taken by pitfall traps (totally 30 pitfall traps) in three different areas. At the first stage, the soil temperature was measured in the field. At the next stage, the soil chemists such as pH, humidity and soil organic, were analyzed in the laboratory. At the area I, the surface soil arthropods collected were from 9 ordo, 26 families, 31 species, and 3609 individuals. Meanwhile at the area II, the arthropods were 12 ordo, 26 families, 31 species, and 2502 individuals. At the last area, the arthropods collected were 12 ordo, 25 families, 28 species and 1272 individuals. Finally, it can be summarized that the arthropod composition on the three surface soils was similar with the similarity index 55.7%. It means the chemical and physical factors of the three areas still optimally supported the surface soil arthropods life cycle.Key words: surface soil, arthropods, composition, coal mining 
JENIS-JENIS ALGA EPILITIK PADA SUMBER AIR PANAS DAN ALIRANNYA DI KAWASAN CAGAR ALAM RIMBO PANTI KABUPATEN PASAMAN Widiana, Rina; Abizar, Abizar; Wahyuni, Sri
Sainstek : Jurnal Sains dan Teknologi Vol 3, No 2 (2011)
Publisher : IAIN Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (33.276 KB) | DOI: 10.31958/js.v3i2.47

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Epilitik algae is part of perifitik microalgae which live by sticking on any subtracts such as; rock, coral, and other solid material under water.  Algae has green pigment which is called chlorophyll so it can do photosynthetic.  Some epilitik algae can adapt in his living place.  Cyanophyta is one of algae which lives in any type of habitas such as; pond, stream even land.  It can live in middle or extreme temperature.  Thus this research was conducted for any kind of epilitik algae in hot water source and its stream in Rimbo Panti reservation in Pasaman.  The research was conducted on June 2010 in survey method.  The sample was identified in Biology laboratory of Biology department in STKIP PGRI West Sumatera.  The concentration measuring of sulfur, nitrogen, and phosphate was done in water laboratory of environment technique department of technical faculty of Andalas university Padang.  The parameter which was used are species. Iden-tification key, description, photo specimen, temperature, pH, the concentration of sulfur, nitrogen, phosphate, and dissolved oxygen (DO).  It was found that there are 11 kinds of epilitik algae where three are categorized as chrysophyta division and eight as Cyanophyta.  In the first station there were 3 epilitik algae and 9 epilitik algae at the second station.  The major species which were found in 90o C are Navicula tenella, Ropalodia gibberula and Oscillatoria minima.  Thus, it was found that the total number of species in each station is different.  Epilitik algae was mostly found in hot water source and only few of them was found in its stream.Key words: epilitik algae, hot water source
DAYA HAMBAT SARI DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav) TERHADAP PERTUMBUHAN BAKTERI ESCHERICHIA COLI DAN Staphylococcus aureus Indriati, Gustina; Agustina, Agustina; Widiana, Rina
Sainstek : Jurnal Sains dan Teknologi Vol 4, No 2 (2012)
Publisher : IAIN Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.087 KB) | DOI: 10.31958/js.v4i2.68

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Sirih merah (piper crocatum ruiz & pav) has been used, since ancient society, as drugs.  The chemical compounds contained in red betel are as antibacterial, the compounds are: flavonoids, alkaloids. tannins and essential oils. The research has been conducted in order to determine the inhibition of red betel leaf extract (piper crocatum ruiz & pav) on the growth of the bacteria Escherichia coli and staphylococcus aureus. This study uses RAL with 6 treatments and 3 replications: A treatment (red betel leaf extract 10%), B (red betel leaf extract 20%), C (red betel leaf extract 30%), D (red leaf extract 40%) , E (red betel leaf extract 50%) and F (amoxicilin 10%) as controls. The results showed no real influence with inhibition diameter on treatment A (6.69 mm), treatment B (8.88 mm), treatment C (7.76 mm), treatment D (9.53 mm), treatment E (12 , 79mm) and treatment F (9.45 mm). From staphylococcus aureus it  was obtained significant results with a diameter of inhibition in treatment C (7.70 mm), treatment A (8.22 mm), treatment D (8.58 mm), treatment B (9.53 mm), treatment E (10.39 mm ) and treatment F (17.59 mm). The red betel leaf extract was able to inhibit the growth of bacteria Escherichia coli and staphylococcus aureusKey words: Sirih merah, Escherichia coli, Staphylococcus aureus
THE EFFECTIVENESS OF ANIMAL PHYSIOLOGY PRACTICAL BOOK BASED ON GUIDED INQUIRY MODEL Widiana, Rina; Susanti, Diana; Susanti, Silvi; Sumarmin, Ramadhan
JPBI (Jurnal Pendidikan Biologi Indonesia) Vol 5, No 3 (2019): November
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jpbi.v5i3.9784

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The effectiveness of animal physiology practical book based on guided inquiry model is the essential factor to achievement student understanding a about animal physiology concepts. The purpose of this study was to observe the effectiveness of animal physiology practical book based on guided inquiry model in Padang State University. The variables measured were affective, cognitive, psychomotor, and students? activities. The data of this pre-experimental research were analyzed using averaged, percentage and standardized with Institutional assessment criteria. The results showed that the mean values were: 90.56 for affective competency (Criteria A), 82 for knowledge (Criteria A), and 89.66 for skills (Criteria A). Meanwhile, the percentage of student activities was 90.74% with very active category. It can be concluded that the effectiveness of practical book based on guided inquiry model was very effective to optimized student competences.
Kepadatan dan Pola Distribusi Polymesoda bengalensis Lamarck di Perairan Muaro Nipah Kabupaten Pesisir Selatan Sumatera Barat RINA WIDIANA; JABANG NURDIN; NOVA AMELIA
Prosiding Seminar Biologi Vol 2 No 1 (2016): Prosiding Seminar Nasional From Basic Science to Comprehensive Education
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v2i1.3315

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Kerang P. bengalensis dari familia Corbiculidae merupakan produk perikanan yang bernilai ekonomi serta potensial untuk dikembangkan.  Di beberapa Negara sudah menjadi komoditi ekspor dengan harga yang mahal, tetapi di Indonesia, terutama di perairan Muaro Nipah Kecamatan Sutera Kabupaten Pesisir Selatan Sumatera Barat, pemanfaatan kerang ini masih sebatas makanan tambahan (lauk) bagi penduduk setempat dan pengambilannya langsung dari habitat serta belum ada usaha budidaya. Sejalan dengan semakin banyaknya informasi dari nilai gizi dan manfaat kerang ini terhadap manusia maka  harga kerang semakin meningkat.  Kondisi ini menyebabkan frekuensi pengambilan oleh masyarakat di lapangan semakin meningkat dan intensif tanpa memperhitungkan potensi lestarinya sehingga ke depannya akan menekan populasi alami dan mengancam kepunahan kerang ini serta seterusnya akan mengganggu keseimbangan ekosistem. Berdasarkan kondisi tersebut, maka perlu dilakukan usaha pengelolaan dan kemungkinan pembudidayaan kerang tersebut.  Atas dasar pemikiran di atas, sebagai langkah awal budidaya maka telah dilakukan penelitian dengan tujuan mengetahui kepadatan populasi dan pola distribusi Polymesoda bengalensis di Perairan Muaro Nipah Kecamatan Sutera Kabupaten Pesisir Selatan Sumatera Barat. Penelitian ini dilakukan dengan menggunakan metode Stratified purposive sampling. Pengambilan sampel dilakukan pada tiga lokasi dan masing-masing lokasi terdiri dari tiga strata. Analisis kepadatan populasi menggunakan rumus (Krebs, 1972) dan pola distribusi menggunakan Indek Morista (Michael, 1994). Hasil penelitian didapatkan kepadatan populasi P. bengalensis 6,4 ind/m2 dengan  pola distribusi seragam dan kondisi fisika kimia lingkungan masih mendukung kehidupan kerang, dimana suhu berkisar antara 28,00 – 31,00 C, pH  7,0, salinitas 4,0 -5,0 0/00, kecerahan 15 cm, KOS 6,8 – 37,9 %, oksigen terlarut 5,2 – 7,0 ppm, komposisi substrat yang diperoleh berpasir dan yang paling dominan adalah pasir sedang. Kata kunci: Kepadatan populasi, pola distribusi, Polymesoda bengalensis, estuari.