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ANALISIS FAKTOR RISIKO KEJADIAN DRY EYE DISEASE PADA TENAGA KEPENDIDIKAN FAKULTAS KEDOKTERAN UNIVERSITAS ANDALAS Febiyola Ratu Khairani; Hendriati Hendriati; Husnil Kadri; Kemala Sayuti; Tofrizal Tofrizal; Moestafa Nur
HUMAN CARE JOURNAL Vol 8, No 3 (2023): Human Care Journal
Publisher : Universitas Fort De Kock

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32883/hcj.v8i3.2691

ABSTRAKDry eye disease (DED) atau penyakit mata kering merupakan penyakit multifaktorial pada mata yang memengaruhi penglihatan, aktivitas sehari-hari, produktivitas kerja, dan kualitas hidup secara umum sehingga penting untuk mengontrol faktor risiko yang dapat dimodifikasi. Penelitian ini bertujuan untuk mengetahui hubungan antara jenis kelamin, usia, penggunaan komputer, penggunaan ruangan ber-AC serta kebiasaan merokok dengan risiko kejadian dry eye disease pada tenaga kependidikan Fakultas Kedokteran Universitas Andalas. Metode yang digunakan adalah analitik observasional dengan desain penelitian cross sectional menggunakan kuesioner OSDI dengan uji korelasi Mann-Whitney dan Rank Spearman. Hasil penelitian menunjukkan bahwa terdapat hubungan yang signifikan antara usia (p=0,012) dan penggunaan komputer (p=0,000) dengan DED dimana dengan bertambahnya usia dan semakin lama durasi penggunaan komputer maka semakin tinggi risiko DED namun tidak terdapat hubungan yang signifikan antara jenis kelamin (p=0,877), penggunaan AC (p=0,463), dan kebiasaan merokok (p=0,206) dengan DED. ABSTRACTDry eye disease (DED) is a multifactorial disease that occurs on the eye. DED symptoms can affect vision, daily activities, productivity at work and general quality of life therefore the modifiable risk factors is important to control. This study was aimed to determined the relationship between gender, age, use of computers, use of air-conditioner rooms, and smoking habits to the risk of dry eye disease in educational staff at the Faculty of Medicine Universitas Andalas. The method used was an observational analytic with a cross sectional design was using the OSDI questionnaire with the Mann-Whitney correlation test and Spearman Rank correlation test. The results of the study showed that there was a significant relationship between age (p=0,012) and computer use (p=0,000) to DED, increasing age and the longer duration of computer use, the higher risk of suffer from dry eye disease but there was no significant relationship between gender (p=0,877), use of air-conditioner (p=0,463), dan smoking habits (p=0,206) to DED.

Hubungan Ekspresi Sodium Iodide Symporter dengan Varian Histopatologi dan Stadium Papillary Thyroid Carcinoma Rini Purnama Sari; yenita - yenita; Noza - Hilbertina; tofrizal - tofrizal; Daan - Khambri; Husna - Yetti
Majalah Kedokteran Andalas Vol 46, No 8 (2024): Online Januari 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i8.p1280-1288.2024

Abstrak Tujuan: Mengetahui hubungan ekspresi sodium iodide symporter dengan varian histopatologi dan stadium papillary thyroid carcinoma. Metode: Penelitian ini merupakan penelitian observasional dengan pendekatan cross sectional. Sampel penelitian ini adalah kasus papillary thyroid carcinoma dari laboratorium Patologi Anatomi RSUP Dr. M. Djamil Padang periode Januari 2022-Desember 2022 sebanyak 37 kasus. Varian histopatologi papillary thyroid carcinoma dinilai berdasarkan WHO 2017 dan stadium klinis dinilai berdasarkan TNM American Joint Committee on Cancer edisi ke VIII. Ekspresi NIS dinilai melalui pulasan imunohistokimia. Analisis bivariat dilakukan dengan uji Chi-square dengan hasil uji statistik dianggap bermakna jika p < 0,05. Hasil: Penelitian ini menunjukkan ekspresi NIS positif sebesar 13,5%. Analisis statistik menunjukkan adanya hubungan yang bermakna antara ekspresi NIS dengan varian histopatologi papillary thyroid carcinoma (p=0,03). Varian histopatologi tertentu menunjukkan tingkat ekspresi NIS yang lebih tinggi dibandingkan dengan varian lainnya. Namun, penelitian ini tidak menemukan hubungan antara ekspresi NIS dengan stadium papillary thyroid carcinoma (p =0,61). Kesimpulan: Terdapat hubungan antara ekspresi NIS dengan varian histopatologi. Ekspresi NIS tidak berhubungan dengan stadium papillary thyroid carcinoma.Kata kunci: sodium iodide symporter; papillary thyroid carcinoma; varian histopatologi; stadium

DIFFERENCES OF FIBRONECTIN LEVELS IN FIRST TRIMESTER NORMAL PREGNANCY AND MISCARRIAGE Maisarah Fadhilah; Bobby Indra Utama; Tofrizal Tofrizal
Andalas Obstetrics And Gynecology Journal Vol. 8 No. 1 (2024)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aoj.8.1.647-652.2024

Introduction: Miscarriage is a reflection of the low quality of health in pregnant women. The incidence of miscarriage in the world accounts for 23 million (15%) of the 130 million births per year and up to 80% of miscarriages occur in the first trimester of pregnancy. Miscarriage will affect a woman's social, physical, and psychological. The complexity of the negative impact of miscarriage makes it necessary to pay special attention. Biomarker examination is needed to more accurately identify pregnancies at risk of miscarriage before the appearance of clinical symptoms. The use of fetal fibronectin (fFN) levels have been used as a marker of unexpected labor and as evidence of premature rupture of the fetal membranes. Normally fFN can be detected in cervical and vaginal secretions at <20 weeks gestation. The presence of fFN at >22 weeks gestation indicates disruption of the uteroplacental surface. Therefore, if screening for fibronectin levels using the Enzyme-Linked Immunosorbent Assay (ELISA) test can be carried out in the first trimester of pregnancy, there is a high possibility that miscarriage can be prevented.Method: The type of research is analytical observational research with a cross-sectional research design. The stored samples were examined for fibronectin levels using the ELISA test, which included 21 blood serum samples from normal pregnancy patients in the first trimester and 21 miscarriage samples.Results: The results of the ELISA test produced an average first-trimester normal pregnancy fibronectin level of 118.8 Â± 18.4 ng/mL while the miscarriage fibronectin level was 208.2 Â± 152.0 ng/mL. Data analysis using the Mann-Whitney test obtained a p-value = 0.138, which means there was no significant difference in fibronectin levels between normal pregnancy in the first trimester and miscarriage.Conclusion: This study concludes that fibronectin levels are not specific biomarkers in detecting miscarriage in the first trimester of pregnancy.

UPAYA PENINGKATAN PENGETAHUAN ORANG TUA TERHADAP ZINC DAN PEMBERIAN ZINC KEPADA SISWA SD BUSTANUL ULUM PADANG Siti Nurhajjah; Nur Afrinin Syah; Afdal Afdal; Dewi Rusnita; Tofrizal Tofrizal; Dwisari Dillasamola; Desmawati Desmawati
BULETIN ILMIAH NAGARI MEMBANGUN Vol. 4 No. 1 (2021)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v4i1.288

Zinc is an essential micronutrient in the human body as a cofactor of about 300 enzymes. Zinc is very instrumental in the growth process, including maintaining the thymus so that atrophy is not fast. Zinc deficiency can affect the immune system. Zinc deficiency can be caused due to a lack of zinc intake. In theory, many factors affect zinc intake, among others, community knowledge, education, economy, diet, habits, and customs. Lambung Bukit Village is a fostered village of Andalas University. Most people in this village are classified as economically deficient because the community generally farms. The level of public knowledge about zinc is still lacking. This service aims to increase public knowledge of the importance of zinc on body health and the provision of zinc supplements to prevent zinc deficiency and is expected to reduce morbidity and mortality due to infection in children. This activity has been carried out at Bustanul Ulum Elementary School in Batu Busuk Village, Lambung Bukit Village, Pauh District, Padang City. Counseling conducted to 48 people (parents of students and teachers). Almost all parents' knowledge is lacking about zinc, the role, source of zinc, and its relation to several diseases. We hope this counseling can increase public knowledge about the importance of zinc on the body, mainly to prevent susceptibility to infections and any food sources that contain zinc. The results of a health examination by pediatricians found two students suffering from Congenital Heart Disease (CHD), poor oral hygiene, and many teeth carious students. Giving zinc syrup to children aged 7-10 years is expected to prevent zinc deficiency in children.

A Rare Case of Multiple Hereditary Exostosis: Making The Correct Diagnosis with Triple Diagnosis Rahmi Ramadhani; Noza Hilbertina; Yenita; Tofrizal; Pamelia Mayorita; Loli Devianti; Meta Zulyati O ktora
Scientific Journal Vol. 4 No. 4 (2025): SCIENA Volume IV No 4, July 2025
Publisher : CV. AKBAR PUTRA MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56260/sciena.v4i4.248

Background: A rare genetic disorder characterized by the development of multiple benign bone tumors called osteochondromas. This condition is primarily hereditary and follows an autosomal dominant pattern of inheritance, with mutations in the EXT1 and EXT2 genes being the most common genetic. Case report: This case report describes an 12-year-old boy with lumps in all four extremities accompanied by intermittent pain. Femur x-rays concluded MHE of the femur, tibia, and bilateral fibula. Histopathological examination shows that the cartilage tissue contains chondrocyte cells that form the cartilage cap. There is a transition of cartilage to bone trabeculae through endochondral ossification and bone marrow between the bone trabeculae. Discussion and Conclusion: MHE is a rare condition, with an incidence of approximately 1 in 50,000 to 100,000 live births. MHE is closely related to genetic mutations that occur in the EXT1, EXT2, and EXT3 genes. These genes code for enzymes involved in the biosynthesis of heparan sulfate, which is necessary for normal bone growth. Mutations in any of these genes disrupt the regulation of bone growth and lead to osteochondroma formation. Symptoms of MHE can range from mild to severe, and they tend to appear in childhood or adolescence. The main clinical manifestations include the formation of a hard lump on the bone, which can cause deformity, pain, and limitation of movement. Osteochondroma can also compress nerves or blood vessels, causing complications such as circulatory disorders or paralysis. We report a rare case emphasizing the typical morphology of multiple hereditary exostosis of the long bones to establish the final diagnosis without immunohistochemistry and molecular examination. In low-resource settings where molecular analysis is not available, histopathological examination are key tools to establish a correct diagnosis.

Diffuse Sclerosing Papillary Thyroid Carcinoma (Ds-Ptc): Kasus Jarang Dengan Perilaku Agresif: Kasus Jarang Dengan Perilaku Agresif Yuliza Ariani; Yessy Setiawatu; Aswiyanti Asri; Tofrizal; Hera Novianti; Meta Zulyati Oktora
Scientific Journal Vol. 4 No. 6 (2025): SCIENA Volume IV No 6, November 2025
Publisher : CV. AKBAR PUTRA MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56260/sciena.v4i6.284

Pendahuluan: Diffuse sclerosing Papillary Thyroid Carcinoma (DS-PTC) adalah subtipe PTC yang jarang terjadi dengan perilaku klinis yang agresif. Adanya ciri-ciri papiler membuat ahli patologi salah mendiagnosis tumor ini sebagai PTC konvensional. Namun, adanya keterlibatan kelenjar getah bening yang menyebar di kelenjar getah bening harus diwaspadai oleh ahli patologi. Laporan Kasus: dilaporkan kasus seorang pria berusia 35 tahun dengan benjolan di lehernya yang semakin membesar dalam 3 tahun, mengeluh sesak napas dan suara serak. Benjolan tersebut memiliki konsistensi padat, berukuran 12x7x5 cm dan 10x6x4 cm, dan mengikuti gerakan menelan. Nilai FSH dan T4 berada dalam batas normal. CT scan leher menunjukkan tumor tiroid kistik (sugestif ganas) dengan limfadenopati koli bilateral. Tiroidektomi bilateral dilakukan. Kelenjar getah bening (KGB) dan jaringan dikirim ke laboratorium anatomi patologis untuk diperiksa. Jaringan tiroid tampak berwarna putih kecoklatan pada penampang melintang dengan beberapa kista. Secara mikroskopis, terdapat proliferasi sel tumor yang membentuk struktur papiler dengan inti yang besar dalam mode tumpang tindih dengan gambaran groove dan ground glass. Ditemukan juga sklerosis stroma, psamoma bodies, dan metaplasia skuamosa, serta infiltrasi limfovaskular dan penyebaran sel tumor ke 26 kelenjar getah bening. Temuan ini konsisten dengan diagnosa DS-PTC stadium minimal pT3aN1bMx. Pasien ditangani dengan radioterapi setelah operasi. Kesimpulan: DS-PTC memiliki insiden metastasis kelenjar getah bening dan metastasis jauh yang lebih tinggi dibandingkan dengan classic PTC.Temuan ini harus diperhatikan oleh ahli patologi saat mendiagnosis tumor.

Loss of E-cadherin Expression Stratifies Aggressive versus Non-Aggressive Papillary Thyroid Carcinoma Dwi Yanti Fioni Putri; Yenita; Aswiyanti Asri; Tofrizal; Rony Rustam; Husna Yetti
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 2 (2026): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i2.1498

Background: Papillary thyroid carcinoma (PTC) is generally indolent, yet specific histological subtypes defined by the World Health Organization (WHO) are linked to aggressive behavior and poor prognosis. The loss of the cell-adhesion protein E-cadherin is a hallmark of the epithelial-to-mesenchymal transition (EMT), a process implicated in tumor aggression. However, its role in stratifying PTC subtypes versus its correlation with tumor stage remains a significant controversy in the literature. This study aimed to disentangle these two parameters by clarifying the relationship between E-cadherin expression and both histological phenotype and tumor stage. Methods: This was an observational, cross-sectional pilot study on 40 randomly selected, formalin-fixed, paraffin-embedded (FFPE) PTC cases from a 2024 cohort (N=74) at a tertiary hospital in Indonesia. All cases were re-evaluated and classified according to the WHO 5th Edition (2022) criteria as non-aggressive (n=34) or aggressive (n=6). E-cadherin expression was assessed by immunohistochemistry (IHC) using a standardized semi-quantitative scoring system (product of intensity and proportion) adapted from previous studies, with inter-rater reliability assessed (Cohen’s Kappa = 0.88). Scores were dichotomized as 'High' (n=25) or 'Low' (n=15). The association between E-cadherin expression and both histological subtype and AJCC 8th Edition tumor stage (Early: I/II [n=32] vs. Advanced: III/IV [n=8]) was analyzed using Fisher's Exact Test, with Odds Ratios (OR) and 95% Confidence Intervals (CI) calculated. Results: High E-cadherin expression was observed in 62.5% of cases. A statistically significant and strong association was found between E-cadherin expression and histological subtype (p=0.021; OR 12.0; 95% CI 1.2–118.9). Low E-cadherin expression was present in 83.3% (5 of 6) of aggressive-subtype tumors, versus only 29.4% (10 of 34) of non-aggressive subtypes. In contrast, no significant correlation was found between E-cadherin expression and advanced tumor stage (p=0.126; OR 3.67; 95% CI 0.7–18.6). Conclusion: Loss of E-cadherin expression is a significant biomarker associated with high-risk, aggressive histological phenotypes in PTC. Its lack of correlation with tumor stage, confirmed by an uncertain OR, suggests E-cadherin's role is indicative of an inherent tumor biological phenotype (aggressiveness) rather than a linear marker of tumor progression (stage). This dichotomy, likely reflecting EMT/MET plasticity, positions E-cadherin IHC as a powerful ancillary tool for pathological risk stratification.

The Large-Mass Phenotype of Uterine Smooth Muscle Tumor of Uncertain Malignant Potential (STUMP): A Clinicopathological Analysis of 37 Cases in Indonesia Using WHO 2021 and Modified Stanford Criteria Yuliza Ariani; Henny Mulyani; Noza Hilbertina; Yenita; Aswiyanti Asri; Tofrizal; Loli Devianti; Hera Novianti
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 4 (2026): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i4.1565

Background: Uterine smooth muscle tumor of uncertain malignant potential (STUMP) presents a diagnostic dilemma, especially in Southeast Asian populations, where delayed presentation often leads to advanced tumor burden. This study aimed to characterize the clinicopathological profile of STUMP in Indonesia using the 2021 WHO Classification and modified Stanford parameters. Methods: A descriptive study analyzed 37 STUMP cases diagnosed between 2023 and 2025 at a tertiary referral center in West Sumatra. A panel of pathologists re-evaluated archival slides for atypia, mitotic activity, necrosis, and growth patterns, establishing inter-observer reliability. Fisher’s Exact Test was employed to correlate tumor size with morphological markers of aggression. Results: The cohort demonstrated a distinct large-mass phenotype, with 54.1% of tumors exceeding 10 cm and a median diameter of 14.5 cm. Pathological review classified 56.8% of cases into WHO Group 4 (atypia with ambiguous mitosis). A statistically significant correlation was identified between tumor size >10 cm and the presence of infiltrative growth margins (81.1%; p=0.034). Conclusion: Indonesian STUMP cases are distinctively characterized by massive size and high rates of infiltrative growth, likely driven by prolonged natural history. The prevalence of ambiguous mitotic figures underscores the utility of the WHO Group 4 category in resource-limited settings. These findings advocate for aggressive surgical management and wider resection margins in large-mass variants to mitigate local recurrence risk.

PENERAPAN TEKNIK PERIODIC ACID SCHIFF (PAS) PADA DIAGNOSIS HISTOPATOLOGI Fajriza Yona; Salmiah Agus; Tofrizal Tofrizal
Collaborative Medical Journal Vol 5 No 1 (2022): Januari
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar

Histopathological tissue examination with routine staining sometimes cannot provide a clear morphology of certain cells or tissues, therefore special staining techniques based on histochemical reactions are needed. Periodic Acid-Schiff's (PAS) is a histochemical staining method specifically used to detect the presence of polysaccharide carbohydrates, neutral mucins and other glycoproteins. This article was written with the aim of increasing knowledge for practitionersor clinicians regarding the PAS technique procedure and how it is used in diagnosis. The method of writing scientific papers is a literature review sourced from various journals, books and scientific articles that are relevant and in accordance with the topics discussed. The PAS technique procedure begins with fixation in 10% neutral buffer formalin, preparation of materials and reagents, PAS staining with counterstain and interpretation. The addition of diastase was performed before PAS stain for PAS/D, and Alcian Blue staining was performed before PAS stain for the AB/PAS technique. Errors at each stage will affect the results of PAS staining. The use of combined PAS and PAS techniques in pathological diagnosis is to exclude the differential diagnosis of various tumors containing glycogen and mucin, examine the thickness of the basement membrane in various diseases, and identify Jamur and amoeba in tissue infections.

Loss of E-cadherin Expression Stratifies Aggressive versus Non-Aggressive Papillary Thyroid Carcinoma Dwi Yanti Fioni Putri; Yenita; Aswiyanti Asri; Tofrizal; Rony Rustam; Husna Yetti
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 2 (2026): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i2.1498

Background: Papillary thyroid carcinoma (PTC) is generally indolent, yet specific histological subtypes defined by the World Health Organization (WHO) are linked to aggressive behavior and poor prognosis. The loss of the cell-adhesion protein E-cadherin is a hallmark of the epithelial-to-mesenchymal transition (EMT), a process implicated in tumor aggression. However, its role in stratifying PTC subtypes versus its correlation with tumor stage remains a significant controversy in the literature. This study aimed to disentangle these two parameters by clarifying the relationship between E-cadherin expression and both histological phenotype and tumor stage. Methods: This was an observational, cross-sectional pilot study on 40 randomly selected, formalin-fixed, paraffin-embedded (FFPE) PTC cases from a 2024 cohort (N=74) at a tertiary hospital in Indonesia. All cases were re-evaluated and classified according to the WHO 5th Edition (2022) criteria as non-aggressive (n=34) or aggressive (n=6). E-cadherin expression was assessed by immunohistochemistry (IHC) using a standardized semi-quantitative scoring system (product of intensity and proportion) adapted from previous studies, with inter-rater reliability assessed (Cohen’s Kappa = 0.88). Scores were dichotomized as 'High' (n=25) or 'Low' (n=15). The association between E-cadherin expression and both histological subtype and AJCC 8th Edition tumor stage (Early: I/II [n=32] vs. Advanced: III/IV [n=8]) was analyzed using Fisher's Exact Test, with Odds Ratios (OR) and 95% Confidence Intervals (CI) calculated. Results: High E-cadherin expression was observed in 62.5% of cases. A statistically significant and strong association was found between E-cadherin expression and histological subtype (p=0.021; OR 12.0; 95% CI 1.2–118.9). Low E-cadherin expression was present in 83.3% (5 of 6) of aggressive-subtype tumors, versus only 29.4% (10 of 34) of non-aggressive subtypes. In contrast, no significant correlation was found between E-cadherin expression and advanced tumor stage (p=0.126; OR 3.67; 95% CI 0.7–18.6). Conclusion: Loss of E-cadherin expression is a significant biomarker associated with high-risk, aggressive histological phenotypes in PTC. Its lack of correlation with tumor stage, confirmed by an uncertain OR, suggests E-cadherin's role is indicative of an inherent tumor biological phenotype (aggressiveness) rather than a linear marker of tumor progression (stage). This dichotomy, likely reflecting EMT/MET plasticity, positions E-cadherin IHC as a powerful ancillary tool for pathological risk stratification.

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