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Ekspresi Protein Sitoplasmik SOCs2 pada Adenokarsinoma Kolorektal: Studi Imunohistokimia Nita Afriani Azmal; Shinta Ayu Intan; Tofrizal Tofrizal
Jurnal Kesehatan Andalas Vol 11, No 1 (2022): Online March 2022
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v11i1.1982

Inflammatory cytokines have a role in triggering cell signals in colorectal cancer. They increase signaling pathways into cells via the Janus kinase pathway (JAK) - signal transducer and activator of transcription (STAT) -suppressor of cytokine signaling (SOCs) JAK-STAT. SOCs2 protein is a cytoplasmic protein that can inhibit activation of JAK-STAT pathway so that it prevents cascade of signals into the nucleus. This protein is thought to have decreased in adenocarcinoma colorectal. Objectives: To reveal the expression of SOCs2 protein in 37 paraffin block samples in Anatomy Pathology Department Medical Faculty Andalas University. Methods: Data were taken from medical records and histopathology was assessed by HE. SOCs2 expression was evaluated by immunohistochemistry using SOCs2 polyclonal antibody (bs-1896R). Cytoplasmic expression was counted using Image J software. Results: The average of age was 56.57 years, 21 samples (55.8%) were male, depth of invasion was mostly in the tunica muscularis (T3), and 7 cases of malignancy (18.9%) were poorly differentiated. SOCs2 expression was 5 cases (13.5%). Conclusion: There was SOCs2 expression in a small proportion of colorectal adenocarcinoma.Keywords: SOCs2 protein, cytoplasmic inhibitor, JAK-STAT, cell signaling

STRUKTUR HISTOLOGI DAN JUMLAH SEL GOBLET PADA SEDIAAN HISTOPATOLOGIS RADANG USUS BESAR DENGAN PEWARNAAN HEMATOXYLIN-EOSIN (HE) DAN PERIODIC ACID-SCHIFF (PAS) Chairani Chairani; Fitra Wahyuni; Tofrizal Tofrizal; Salsabila Salsabila
Ensiklopedia of Journal Vol 4, No 3 (2022): Vol 4 No. 3 Edisi 2 April 2022
Publisher : Lembaga Penelitian dan Penerbitan Hasil Penelitian Ensiklopedia

Abstract: Goblet cells and the mucus they produce serve as an important barrier to prevent pathogens invading the mucosal tissue that can cause inflammation. In the inflamed tissue, will have changed in the structure and number of goblet cells. Hematoxylin-Eosin (HE) staining is a standard stain used to examine the histological structure of tissues while Periodic Acid-Schiff (PAS) is a histological stain other than HE that can stain carbohydrates and carbohydrate-rich macromolecules. The purpose of this study was to observe differences in the structure and number of goblet cells in the inflammatory colon using HE and PAS staining. This study used 27 samples of paraffin block of rat colon tissue which were divided into three groups, namely the normal group, acute intestinal inflammation, and chronic intestinal inflammation. The tissue samples were then stained with HE and PAS staining techniques. In this study, statistical analysis of an independent t-test and Mann-Whitney test was used to observe differences in the number of goblet cells in inflammatory colon tissue using HE and PAS staining in each group. This study concludes that the number of goblet cells that can be observed in inflammatory colon tissue using HE and PAS staining is significantly different with a p-value <0.05 where the average number of goblet cells using PAS staining is higher than using HE.Keywords: goblet cells, inflammatory colon, Hematoxylin-Eosin, Periodic Acid-Schiff.

Hubungan Jumlah Eosinofil dengan Ukuran Polip Nasal dan Karakteristik Pasien yang Didiagnosis di Sentra Diagnostik PA FK-UNAND Tahun 2017-2019 Salma Yuri Khairunnisa; Tofrizal Tofrizal; Netti Suharti
Jurnal Ilmu Kesehatan Indonesia Vol 2 No 1 (2021): Maret 2021
Publisher : Fakultas Kedokteran, Universitas Andalas

Background: Nasal polyps is a soft masses filled with fluid in nasal mucosa. The incidence is not high, but nasal polyps are related with inflammatory process. Factors that can affect nasal polyps are eosinophils, polyp size, age and gender of the patient. Objective: This study aims to discuss the association between eosinophils with polyp size, age and gender of polyp patient. Methods: The type of this study was an analytic using a cross sectional approach. This research took place from February- October 2020 in the Pathology Anatomy Diagnostic Central of Faculty of Medicine, Andalas University with 19 total sample and this study used total sampling technique. Results: The results based on bivariate analysis of Chi-Square test between amount of eosinophils with polyp size, age and gender of polyps patients have p values (p = 0, 036), (p = 0.264), (p = 1, 00). Conclusion: There is a significant relationship between eosinophils and polyp size. There is no significant relationship between eosinophils with age and gender of patients with nasal polyps in the Pathology Anatomy Diagnostic Central of Faculty of Medicine, Andalas University in 2017- 2019.

Profil Pasien Deep Vein Thrombosis di RSUP Dr. M. Djamil Padang Tahun 2018 Muhammad Farhan; Masrul Syafri; Tofrizal Tofrizal
Jurnal Ilmu Kesehatan Indonesia Vol 2 No 2 (2021): Juni 2021
Publisher : Fakultas Kedokteran, Universitas Andalas

Latar Belakang. Deep Vein Thrombosis (DVT) merupakan sebuah kondisi oklusi yang disebabkan oleh terbentuknya trombus patologis pada sirkulasi vena dalam tubuh. DVT merupakan sebuah penyakit yang dapat dicegah namun masih memiliki tingkat morbiditas dan mortalitas yang tinggi. Pemeriksaan menggunakan Ultrasonografi (USG) Doppler merupakan metode yang direkomendasikan untuk menegakkan diagnosis DVT. Objektif. Tujuan penelitian ini adalah untuk mengetahui profil dari pasien DVT di RSUP Dr. M. Djamil Padang pada tahun 2018. Metode. Penelitian ini merupakan penelitian deskriptif observasional dengan pendekatan cross sectional melalui pengumpulan data dari hasil pemeriksaan USG Doppler dan rekam medis. Background. Deep Vein Thrombosis (DVT) is an occlusion caused by patological thrombus that formed at deep vein circulation of the body. DVT is a preventable disease but the morbidity and mortality rate of DVT are still high. The recommended method for diagnosing DVT is by examination with Doppler Ultrasonography (USG) for identificating thrombus at deep vein circulation. Objective. The objective from this study is to identifying clinical profile of DVT patient at Dr. M. Djamil General Hospital Padang in 2018. Method. This is a descriptive observational research with cross sectional method by collecting data from Doppler USG examination report and patient medical record. Results. From this research we found 66 DVT patient, most patients were in the age group of 56-65 years old (23 patients), most thrombus located at lower extremities (49 patients), especially at femoral vein (29 patients). Mostly patients shown with swelling at thrombus location (59 patients), and immobilization was the most risk factor found in the patients (58 patients.) Conclusion. From this research we conclude that mostly DVT patients at M. Djamil General Hospital Padang are elderly and mainly women, mostly DVT located at left leg especially in femoral and poplitea vein and shown with swelling at leg, and mostly DVT patients were having immobilization and malignancy as risk factor. Keywords : Deep vein thrombosis, Doppler USG, clinical profile Hasil. Pada penelitian ini didapatkan 66 pasien DVT di RSUP Dr. M. Djamil Padang pada tahun 2018. Kelompok usia tertinggi ditemukan pada rentang usia 56-65 tahun sebanyak 23 pasien. Lokasi trombus paling sering ditemukan pada tungkai sebanyak 49 pasien, terutama pada vena femoralis sebanyak 29 pasien. Manifestasi klinis yang paling sering ditemukan berupa pembengkakan sebanyak 59 pasien, dan faktor risiko yang paling sering ditemukan imobilisasi sebanyak 58 pasien. Kesimpulan. Kebanyakan pasien DVT di RSUP Dr. M. Djamil Padang berusia lanjut serta memiliki DVT pada tungkai terutama pada vena femoralis sebelah kiri dengan manifestasi pembengkakan, dan memiliki faktor risiko imobilisasi. Perlunya penerapan profilaksis DVT pada pasien yang memiliki resiko tinggi untuk menekan angka kejadian DVT. Kata kunci: Deep vein thrombosis, USG Doppler, profil klinis

Effect of Giving Young Papaya (Carica Papaya L.) Fruit Extract on Endometrial Histology of Female Rats (Rattus Norvegicus) Cecen Suci Hakameri; Tofrizal; Elly Usman
Science Midwifery Vol 9 No 1, Oktober (2020): Science Midwifery
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Young papaya contains several phytochemicals such as saponins, alkaloids and flavonoids. The active compounds in young papaya are thought to be able to cause disruption of the hypothalamic-pituitary pathway which causes disruption of GnRH secretion and affects the ovarian cycle which affects the normal endometrial cycle. The aim of this study was to determine the effect of giving young papaya fruit extract (Carica papaya L.) on endometrial histology of female rats (Rattus norvegicus). This study uses the Post Test Only Control Group method. Total samples were 24 rats (Rattus norvegicus), 2-3 months old and 200-300 grams in weight. Samples were taken randomly, divided into 4 groups. Group (K) without being given treatment. Group (P1) was given 200 mg / 200gr BW of young papaya extract. Group (P2) was given young papaya fruit extract 400 mg / 200gr BW. Group (P3) was given young papaya fruit extract 600 mg / 200gr BW. After 20 days of treatment, the endometrial thickness and density of the endometrium were measured. The results showed that the administration of young papaya fruit extract (Carica papaya L.) caused changes in the thickness of the endometrium and inhibited endometrial gland density. The results of statistical analysis using the one way ANOVA test showed the effect of giving papaya extract on endometrial thickness (p = 0.009) and the effect of giving papaya extract on gland density. It can be concluded that there is an effect of giving young papaya (Carica papaya L.) fruit extract on endometrial thickness and endometrial gland density.

The Effect of Giving Fermented Milk Starter Lactococcus lactis D4 Dadih on Obstructive Jaundice: An In Vivo Study Syahriandra; M. Iqbal Rivai; Irwan; Ade Sukma; Tofrizal
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 15 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i15.691

Background: Every 1 person out of 3 people with obstructive jaundice will die from complications of obstructive jaundice. The absence of bile salts causes the loss of the protective layer of the intestinal mucosa, which causes an increase in the permeability of the intestinal wall, triggering the process of translocation of various bacteria from the intestinal tract to the blood circulation so that it can trigger septicemia and sepsis. Dadih or commonly known as ampiang dadih is a fermented buffalo milk product. Dadih contains lactic acid bacteria microbiota, which can function as probiotics fermented milk starter Lactococcus lactis D4 dadih to small intestine in obstructive jaundice in vivo. Methods: This study was an in vivo experimental study using 30 wistar rats, which were divided into 3 test groups. Observation of the condition of the small intestine was carried out histopathologically to assess the inflammatory process and the condition of the intestinal mucosa. Data analysis was performed using SPSS version 25 using univariate and bivariate methods. Results: Group 3 showed minimal histopathological inflammation compared to the control and was statistically different, p<0.05. Conclusion: Giving fermented milk starter Lactococcus lactis D4 dadih was able to reduce the inflammatory response and repair the intestinal mucosa in conditions of obstructive jaundice in vivo studies.

Hubungan Tumor Infiltrating Lymphocytes dengan Jenis Kelamin, Usia, dan Tipe Histopatologi Karsinoma Azzahra Velia; Tofrizal Tofrizal; Husnil Kadri
Jurnal Ilmu Kesehatan Indonesia Vol 2 No 4 (2021): Desember 2021
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v2i4.434

Latar belakang: Karsinoma tiroid merupakan keganasan pada kelenjar tiroid yang paling sering dijumpai di organ endokrin. Beberapa faktor yang berhubungan dengan karsinoma tiroid adalah jenis kelamin, usia, dan tipe histopatologi. Pada proses pembentukan sel kanker, terjadi mekanisme inflamasi dan proliferasi yang menyebabkan tubuh mengeluarkan imun pada area tersebut. Limfosit yang berperan pada respon imun seluler kanker disebut Tumor Infiltrating Lymphocytes (TILs). Objektif: Mengetahui hubungan TILs dengan jenis kelamin, usia, dan tipe histopatologi karsinoma tiroid. Metode: Penelitian ini merupakan penelitian analitik dengan desain pendekatan cross sectional. Pengambilan sampel dilakukan dengan teknik total sampling pada penderita karsinoma tiroid tahun 2018-2019 di Sentra Diagnostik Patologi Anatomi Fakultas Kedokteran Universitas Andalas dari bulan Februari-Desember 2020. Hasil: Hasil penelitian berdasarkan analisis uji bivariat Chi-square antara TILs dengen jenis kelamin (p=0.306), usia (p=0.306), dan tipe histopatologi karsinoma tiroid (p=0.034). Kesimpulan: Kesimpulan penelitian ini adalah terdapat hubungan bermakna antara TILs dengan tipe histopatologi karsinoma tiroid. Selanjutnya, tidak terdapat hubungan bermakna antara TILs dengan jenis kelamin dan usia penderita karsinoma tiroid.

The Effect of Giving Fermented Milk Starter Lactococcus lactis D4 Dadih on Obstructive Jaundice: An In Vivo Study Syahriandra; M. Iqbal Rivai; Irwan; Ade Sukma; Tofrizal
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 15 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i15.691

Background: Every 1 person out of 3 people with obstructive jaundice will die from complications of obstructive jaundice. The absence of bile salts causes the loss of the protective layer of the intestinal mucosa, which causes an increase in the permeability of the intestinal wall, triggering the process of translocation of various bacteria from the intestinal tract to the blood circulation so that it can trigger septicemia and sepsis. Dadih or commonly known as ampiang dadih is a fermented buffalo milk product. Dadih contains lactic acid bacteria microbiota, which can function as probiotics fermented milk starter Lactococcus lactis D4 dadih to small intestine in obstructive jaundice in vivo. Methods: This study was an in vivo experimental study using 30 wistar rats, which were divided into 3 test groups. Observation of the condition of the small intestine was carried out histopathologically to assess the inflammatory process and the condition of the intestinal mucosa. Data analysis was performed using SPSS version 25 using univariate and bivariate methods. Results: Group 3 showed minimal histopathological inflammation compared to the control and was statistically different, p<0.05. Conclusion: Giving fermented milk starter Lactococcus lactis D4 dadih was able to reduce the inflammatory response and repair the intestinal mucosa in conditions of obstructive jaundice in vivo studies.

Platelet Rich Plasma's Use on Recovering Achilles Tendon Ruptures of Wistar Strain White Mouse Seprianto Seprianto; Rizki Rahmadian; Benni Raymond; Tofrizal Tofrizal
Indonesian Journal of Multidisciplinary Science Vol. 2 No. 5 (2023): Indonesian Journal of Multidisciplinary Science
Publisher : International Journal Labs

Achilles tendon is the most common site of lower extremity tendon rupture. Current treatment has not been able to restore the biological and mechanical properties of the tendon to pre-injure condition. One of the treatments used to improve Achilles tendon healing is the application of PRP. The research would like to determine the effect of using Platelet Rich Plasma (PRP) on the recovery of tendon rupture in animals model (wistar strain white rats). This is an experimental study with a post-test only control group design using animals model (white rats). This study was conducted at the INA Lab Laboratory, Padang in November 2022. The samples were divided into two groups, the control group and the treatment group where both of these groups were injured and sutured to the tendons. The control group was not given PRP, while the treatment group was given 0.1 cc of PRP. The PRP used was heterologous from healthy human blood. Observations were made after 1 week both groups were maintained and treated. There were 12 white rats which were divided into the PRP group and the control group. The PRP group had a lower histopathological score (5.83) than the control group (10.50). In this study, there was a significant difference between the recovery of tendon ruptures in animal model (white rats) that were given Human Platelet Rich Plasma (PRP) and experimental animals (rats) that were not given PRP (p value 0.007). PRP improves healing in Achilles tendon rupture.

The Association of Fibulin-2 Expression with Histopathological Grade and Fibrotic Tumor Vessel in Meningioma Muthia Kamelia; Salmiah Agus; Tofrizal Tofrizal
Majalah Patologi Indonesia Vol. 32 No. 2 (2023): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v32i2.622

Background Meningiomas are the most common primary tumors of the central nervous system. About one-fifth of meningiomas tend to recur. The World Health Organization (WHO) histopathological grade was the most useful morphological predictor of recurrence. Fibrotic tumor vessels (FTV) were detected in nearly half of WHO grade I meningiomas and correlated with vascular density and increased risk of recurrence. Fibulin-2 is an extracellular matrix glycoprotein whose expression increases in traumatic central nervous system injuries and can be used as a marker to differentiate the histopathological grade of meningiomas. This study aimed to determine the association of fibulin-2 expression with histopathological grade and FTV in meningiomas. Methods This research was an observational cross-sectional study. The study sample was 36 cases of meningioma in three Anatomical Pathology laboratories in West Sumatra on period January 2019 to December 2020. Samples obtained from resected meningioma cases were then reevaluated on Hematoxylin Eosin (HE) slides to determine the histopathological grade and FTV. Van-Gieson staining was performed to confirm FTV. Fibulin-2 expression in tumor cells was analyzed using immunohistochemical staining. Bivariate statistical analysis using the Chi-Square test with p<0.05 was considered significant. Result High-risk meningiomas (WHO grade II and III) showed high fibulin-2 expression (62.5%), whereas low-risk meningiomas (WHO grade I) were more abundant with low fibulin-2 expression (75%). The presence of FTV at low fibulin-2 expression was 58.3%. Statistical analysis showed no significant association between fibulin-2 expression with histopathological grade (p=0.077) and FTV (p=1,000). Conclusion In summary, the study showed that fibulin-2 expression was not associated to histopathological grade nor to FTV in meningiomas.

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