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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences ENVIAGRO Jurnal Sumberdaya Hayati Biopropal Industri BERITA BIOLOGI Jurnal Penelitian Karet ANNALES BOGORIENSES Makara Journal of Science Annales Bogorienses
UTUT WIDYASTUTI
Institut Pertanian Bogor, Jl. Raya Dramaga, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology

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Molecular Marker-Assisted Selection of Rice Grain Quality on Rice (Oryza sativa L.) Lines Tolerant to Fe Toxicity Stress Utami, Silvia; Widyastuti, Utut; Utami, Dwinita Wikan; Rosdianti, Ida; Lestari, Puji
Journal of Tropical Life Science Vol 7, No 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.13

Abstract

The elite rice has been produced, including iron (Fe) tolerant varieties. To get the appropriate rice lines which superior not only Fe tolerant but also have good grain quality needs to be developed selection system, especially in the use of molecular markers. This study was aimed to develop molecular markers for selection the rice grain quality characters of selected rice lines Fe tolerant. A total of 30 selected Fe tolerant rice lines and 5 parents as control lines were used in this research. Characterization of grain quality were quantitatively using the standard. While for genotyping analysis used 19 molecular markers of STS, SSR, Indel and SNP. This study showed that 14 of 19 markers result polymorphic DNA band (DNA markers). Association analysis of genotype and phenotype showed that 10 of 14 markers were significantly (p < 0.05) related to high quality of rice grain. Among four types of markers used in this study, STS was the most widely associated significantly with four characters of rice quality. The phenotyping analysis showed that the physical grain and palatability quality which obtained from the total mean of 30 rice lines tested tend to nearly with the parent’s value as controls lines. The most of these lines were included in the group IV of National Rice Grain Quality Standard (SNI). The amylose content (AC) showed that the texture was varied from firm and dry (high AC) to soft and sticky (low AC). The association results showed that there were significant (p ≤ 0.05) markers related with the biosynthesis starch genes, i.e: SBE1, SS1, SSIIa, GPA, PUL and S3cl which contributed on the character of rice palatability. These selected significant markers could be useful for screening of other population with Fe tolerant and/ or other desired morpho-agronomical traits in support of rice breeding program in Indonesia.
Co-Authors . Syafarudin, . AKIHO YOKOTA ALEX HARTANA Ardiwinata Ardiwinata, Ardiwinata Aris Tjahjoleksono Asep Nugraha, Asep Asri Pirade Paserang Didy Sopandie DWI SUSILANINGSIH DWINITA WIKAN UTAMI Dyah Iswantini Eries Dyah Mustikarini Fadillah, Wendi Nurul Hadi Susilo Hamim Hamim Hani Susanti, Hani Ida Rosdianti, Ida Idha Susanti Indradewa, Rhian KINYA AKASHI Nampiah Sukarno Nurita Toruan-Mathius Nurwijayanti Ochieng, Fredrick Onyango Putra, Sukma Triperdana RADITE TISTAMA ROHANI CINTA BADIA GINTING Suharsono Suharsono Suharsono, Sukmarayu P. Gedoan Sulistyaningsih, Yohana C Swastika, I Nengah Tiwit Widowati, Tiwit Ulfah Juniarti Siregar Umoro, Asahedi Urip Sayekti Widyaningrum, Dian Noverita windarsih, gut Yanti Rosa, Yanti Yuniati, Ratna