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All Journal Buletin Peternakan Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) agriTECH Animal Production : Indonesian Journal of Animal Production Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan Jurnal Kedokteran Hewan
Budi Prasetyo Widyobroto
Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary Other

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THE PERFORMANCE OF MILK PRODUCTION, TOTAL MILK REVENUE AND REPRODUCTION INDICATORS ON DAIRY SMALLHOLDERS IN YOGYAKARTA AND EAST JAVA, INDONESIA Sudi Nurtini; rochijan rochijan; Budi Guntoro; Budi Prasetyo Widyobroto; Indratiningsih Indratiningsih; Nafiatul Umami
Buletin Peternakan Vol 41, No 2 (2017): BULETIN PETERNAKAN VOL. 41 (2) MEI 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i2.23152

Abstract

The purpose of this study was to analyze the performance of milk production, total milk revenue and reproduction indicators of Friesian Holstein Crossbred cows maintained under smallholder’s management system of 122 cows in DIY Province and 345 cows in East Java Province, Indonesia. The study was conducted with survey method and direct observation. A total of 180 Friesian Holstein Crossbred farmers (90 farmers were in DIY and another 90 farmers were from East Java Province) was randomly selected and interviewed used structured questionnaire to assess the milk production, total milk revenue and indicators of reproduction of Friesian Holstein Crossbred cows. The result of the study showed that the average mature equivalent of milk production was 3,810.21±920.10 L/lactation in DIY and 3,717.79±818.44 L/lactation in East Java Province, and the total milk revenue was 12,401,917.87±2.48 IDR per lactation in DIY and 14,647,217.80±3.05 IDR per lactation in East Java Province; day to first mating (postpartum mating), services per conception and days open were 62.34±29.24 days, 2.60±1.32 and 88.58±34.43 days in DIY and 60.62±23.07 days, 2.46±1.26 and 88.67±28.86 days in East Java Province. Based on total milk revenue, the conclusion of this study was Friesian Holstein Crossbred cows maintained under smallholder’s management system in East Java Province better than those in Yogyakarta province (DIY). The mature equivalent of milk production and reproduction indicators of dairy cows (postpartum mating, service per conception and days open) from both provinces showed no significant differences and the value of each parameter reproduction indicators is still within the normal range.
SRY Gene Marker Differences in Native and Crossbreed Cattle Tety Hartatik; Dwi Ahmad Priyadi; Ali Agus; Sigit Bintara; I Gede Suparta Budisatria; Panjono Panjono; Budi Prasetyo Widyobroto; Yudi Adinata
Buletin Peternakan Vol 42, No 3 (2018): BULETIN PETERNAKAN VOL. 42 (3) AUGUST 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i3.29940

Abstract

This study focused on the promoter region of the SRY gene with 1,281 bp DNA fragments, including 5'UTR, CAAT signal, and TATA box. Genomic samples of 19 cattle were obtained from Wagyu-BX (n = 2), BX (n = 5), Simmental (n = 2), Limousin (n = 2), Ongole (n = 2), Madura (n = 2), Bali (n = 2), Nellore (n = 1), and Hereford (n = 1). Two flanking primers (forward and reverse) were used for polymerase chain reaction (PCR). The PCR products were then sequenced by using a two-way primer. The obtained sequences were aligned with clustalW software to determine the differences in the nucleotide base arrangement which compiled the promoter region of the SRY gene. The cattle crossbreeding was done as an effort to improve the genetic variations and qualities. The SRY gene is a marker gene inherited from the male side (bull), so the SRY gene is expected to be used as a marker to monitor the crossbreeding. The monitoring of the crossbreed cattle is an initial effort to increase the genetic variations and enhance the genetic qualities without threatening the germplasm purity. The results of this study showed that the overall sample is monomorphic, except for Bali and Nellore cattle. Further research is needed by expanding the analysis area of the SRY gene and increasing the number of samples.
Effect of Cashew Nutshell Oil Supplementation as Phenol Source for Protein Protection on In Vitro Nutrient Digestibility Rahma Fitriastuti; Lies Mira Yusiati; Budi Prasetyo Widyobroto; Zaenal Bachruddin; Chusnul Hanim
Buletin Peternakan Vol 43, No 4 (2019): BULETIN PETERNAKAN VOL. 43 (4) NOVEMBER 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i4.35591

Abstract

The current study was aimed to evaluate the ability of phenol content in cashew nutshell liquid (CNSL) in binding protein and its effect on in vitro nutrient digestibility. This research divided into three steps, there were determination of total phenol and tannins content in CNSL, optimalization binding of CNSL tannin to bovine serum albumin with the determination of protein-percipitable phenolics compound and protein content using Lowry method, after that amount of optimalization supplementation of CNSL was used for measuring on in vitro nutrient digestibility by Tilley and Terry method. The results showed that phenol and tannin content of CNSL were 148.69 g/100 ml and 28.3 g/100 ml. One gram tannin in CNSL could be bound by 65.83 g protein. Optimum tannin were used in this study was 5.85 g tannin or equivalent to 163.91 mg of CNSL. Amount of CNSL were used in various levels, there are 0, 50, 100, 150 dan 200 mg. CNSL supplementation reduced dry matter, organic matter crude protein digestibility in rumen, and didn’t reduce dry matter dan crude protein in vitro post rumen digestibility. Supplementation of CNSL increased feed efficiency on in vitro.
Lactation Characteristic of Etawah Crossed Breed Goats Under Intensive Management Yuni Suranindyah; Budi Prasetyo Widyobroto; Sulvia Dwi Astuti; Tridjoko Wisnu Murti; Adiarto Adiarto
Buletin Peternakan Vol 44, No 1 (2020): BULETIN PETERNAKAN VOL. 44 (1) FEBRUARY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i1.44176

Abstract

The study aimed to describe lactation characteristic of Etawah Crossed Breed goats raised under intensive management. The study located in BBPTU HPT Baturraden, Purwokerto, used 27 female pregnant goats, at 24 to 27 months old. The goats were managed followed standard operating procedure of the research institute. The data consisted of body size and body weight, colostrum and milk yield, lactation period and reproductive performance. The results showed the average of daily milk yield, lactation period, total milk and colostrum production in first lactation were 960±340 mL/day, 157±41 days, 114,720±68,900 mL and 415±240 mL/day. The proportion of monthly milk production from initial lactation to dry off were 21.27, 22.17, 20.18, 17.29, 12.52, 9.13, and 5.65% of total lactation yield, respectively. Peak production ranged from first to second months of lactation, the highest was in the week 4th, averaged of 1080 mL/day. Production persistency was 66.39%. The rate of increase toward peak was 5.60%/week and rate of decline from peak was -3.92% of milk yield in the previous week. Trend equation of lactation curve was Y = 8412.7 –233.65 x. Means period of postpartum mating, service per conception and body condition score were 88.7±39.2 days, 1.2 and 3.45, respectively. There was no correlation between postpartum mating, body condition score and milk production. The study concluded that Etawah Crossed Breed goat under intensive management had short lactation period, early peak production, low persistency. The dry period was estimated 3 months and pregnancy occurred after peak production. 
Morphometry of Male Reproductive Organ and Sperm Evaluation of Belgian Blue Crossbreed Cattle as a Candidate of Superior Bull Riyan Nugroho Aji; Panjono Panjono; Ali Agus; Budi Prasetyo Widyobroto; Tety Hartatik; I Gede Suparta Budisatria; Ismaya Ismaya; Akhmad Fathoni; Dwi Astuti; Sigit Bintara
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.45944

Abstract

The purpose of this study was to observe the size of reproductive organ and to evaluate the sperm quality of Belgian Blue (BB) crossbreed cattle. This cattle are the crossbreeding result between a female Brahman Cross (BX) cattle inseminated with frozen semen from pure male BB cattle, by Artificial Insemination (IB) method, as a candidate of superior bull. The samples of this study were 2.5 years old (BB1), (BB2), and (BB3) crossbreed cattle. Its process involved general physical examination, genital organ inspection, sperm production, and quality assessment. The data obtained were analyzed through descriptive analysis. The results of this study were the three BB crossbred cattle showed the good appearance of normal reproductive organs with scrotum circumferences of each bull were 40.2 cm, 38.6 cm, and 39.8 cm respectively, and sperm quality of the good and the normal motility values of each bull were 82%, 81% and 80%, viability 84%, 83%, and 86%, and the sperm concentrations of each bull were 2.218 million/ml, 1.986 million/ml and 2.120 million/ml respectively. As a result, it could be concluded that those three BB crossbreed cattle are suitable for being used as a superior bull.
The Effect of Nutmeg Leaves Tannin (Myristica fragrans Houtt) as Protein Protecting Agents on In Vitro Nutrient Digestibility Monica Canadianti; Lies Mira Yusiati; Chusnul Hanim; Budi Prasetyo Widyobroto; Andriyani Astuti
Buletin Peternakan Vol 44, No 1 (2020): BULETIN PETERNAKAN VOL. 44 (1) FEBRUARY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i1.47976

Abstract

This experiment was aimed to study the effect of nutmeg leaf tannin addition on in vitro nutrient digestibility. Treatments in this experiment consisted of: P0 (control without tannin), P1 (feed + 2% tannin) and P2 (feed + 4% tannin). Feed for fermentation substrate consisted of Pennisetum purpureum and soybean meal with ratio 60:40. Fermentation was carried out using Tilley and Terry two stages in vitro technique for 48 hours. Variables measured were the digestibility of dry matter, organic matter, and crude protein in the rumen as well as the total digestive tract digestibility based on in vitro technique. The data obtained were analyzed by One Way ANOVA, and followed by the Duncan Multiple Range Test (DMRT). The results showed that rumen dry matter digestibility was lower (P<0.05) in P1 and P2 (59.03±3.24 and 57.19±1.32) compared to P0 (70.77±1.05), but did not show a significant difference (P>0.05) in the total dry matter digestibility of P0, P1, and P2 (74.88±5.28, 67.70±3.21, and 64.83±4.96). Organic matter digestibility in the rumen was also lower (P<0.05) in P1 and P2 (55.55±6.29 and 55.76±6.88) compared to P0 (75.39±0.91), but did not show significant difference (P>0.05) in total organic matter digestibility from P0, P1, and P2 (64.69±6.44, 64.33±6.34, and 61.20±5.11). The digestibility of crude protein in the rumen at P1 and P2 (45.48±5.12 and 38.47±3.44) was also significantly lower (P<0.05) compared to P0 (60.93±9.72), whereas total digestibility did not show any significant difference (P>0.05). Addition of tannin leaf nutmeg 2% optimally reduced rumen dry matter and crude protein digestibility without causing excessive negative impact on results of in vitro digestibility, so it can be used as a protective agent protein feed.
Identification of Glucogenic Amino Acids Content in Gliricidia maculata as an Alternative Energy Source for High-Yielding Periparturient Dairy Cows Sulvia Dwi Astuti SW; Budi Prasetyo Widyobroto; Ali Agus; Lies Mira Yusiati
Buletin Peternakan Vol 44, No 2 (2020): BULETIN PETERNAKAN VOL. 44 (2) MAY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i2.54103

Abstract

Gliricidia maculata which has long been used as a forage for animal feed may contain a variety of glucogenic amino acids variants. The objective of this study was to identify the glucogenic amino acids content in Gliricidia maculata as an alternative source of energy for high-yielding periparturient dairy cows. The samples were the edible portion of plants, harvested randomly at the optimal cutting age (80 days), from the feed plant collection garden of The Faculty of Animal Science, Universitas Gadjah Mada. Lyophilization was carried out by drying the samples at 55°C for 3 x 24 hours continouesly in a Sanyo Drying Oven MOV-112. Pulverization was done by the Foss Tecator CyclotecTM 1093 Sample Mill with 300 mesh (1 mm screen). The hydrolysis of amino acid was carried out using HCl solution while amino acid derivatization used O-phtalaldehyde (OPA) solution. Separation, determination, and quantification of amino acid were carried out by an analytical method in gradient elution using the Thermo ScientificTM DionexTM UltiMateTM 3000 UHPLC Systems with Rapid Separation Fluorescence Detector. Result showed there were at least fourteen kinds of amino acids identified from the samples, i.e.: aspartic acid, glutamic acid, serine, histidine, glycine, arginine, alanine, tyrosin, methionine, valine, phenylalanine, isoleucine, leucine and lysine. Gliricidia maculata contains 1349 ppm glucogenic amino acids, 412.7 ppm ketogenic amino acids and 444.7 ppm glucogenic and ketogenic amino acids. Moreover, there were three types of glucogenic amino acids with the highest concentration were serine (288.7 ppm), glutamic acid (245.5 ppm) and phenylalanine (197.1 ppm) respectively. Glucogenic amino acids can be used as an energy source for dairy cows through gluconeogenesis. This study suggests that Gliricidia maculata may use to supply the precursors of energy for high-yielding periparturient dairy cows to prevent ketosis.
Effect of Dietary Rumen Undegraded Protein (RUP) Level on Nutrient Intake and Digestion of Lactating Dairy Cows Andriyani Astuti; Rochijan Rochijan; Budi Prasetyo Widyobroto
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.59155

Abstract

The objective of this study was to examine the effect of different levels of rumen undegraded protein (RUP) in diets of lactating dairy cows on feed intake and nutrient digestion. The experiment was run according to completely randomized design with three treatments of complete feed rations containing different levels of rumen undegraded protein (RUP) and four replications for each treatment. In a digestibility trial, twelve Friesian Holstein cows were divided into three groups at each group consist of four cows and given three dietary treatments containing three levels of RUP, namely 7.43% of total crude protein /CP (P0), 8.49% of total CP (P1), and 9.45% of total CP (P2). Diets consisted of forages (King grass, Pennisetum hybrid) and concentrates at ratio of 50:50 in dry matter basis. The diets contained 18.25% CP and 61.75% total digestible nutrient (TDN). The cows fed diet (P0) were considered the control group. Drinking water was given ad libitum. This experiment was conducted for 21 days of adaptation period and seven days of collection period. In the collection period, samples of feed, refusal feed, and faecal were collected to get the chemical content includes dry matter (DM), organic matter (OM), ether extract (EE), crude fiber (CF), crude protein (CP), nitrogen-free extract (NFE) and total digestible nutrients (TDN). The data obtained were processed with one-way ANOVA (analysis of variance) and the difference existed between the means (P<0.05) would be analyzed with Duncan’s new multiple range test. The results showed that cows fed diet P2 (9.45% RUP in CP) in had significantly higher feed and nutrient intake (DM, OM, CP, CF, EE, TDN) and higher DM and OM digestibility compared to the other two treatments.
The Effects of Cassava Pomace and Protected Soybean Meal on Dairy Milk Production and Quality Basofi Sabastian; Budi Prasetyo Widyobroto; Andriyani Astuti
Buletin Peternakan Vol 45, No 2 (2021): BULETIN PETERNAKAN VOL. 45 (2) MAY 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i2.64907

Abstract

  The objective of the study was to determine the effect of cassava pomace and protected soybean meal on dairy milk production and quality of mid lactating dairy cows. This research was conducted at Turen, Malang from January to April 2020. Twelve lactating Friesian Holstein dairy cows were divided into 2 groups so that each group consisted of 6, namely the control and treatment groups. The feed were a combination of forage and concentrate with a ratio of 35:65 in dry matter (DM). The control group received wet concentrate with DM content of 30.58%, 14.87% CP, and 75.06% TDN, while the treatment groups received concentrate with supplementation of cassava pomace 15% of DM ratio and protected soybean meal 45 g/l milk production. The variables observed were nutrient consumption, milk production and quality. Data between the two groups were analyzed using the Independent Sample T-test. The results showed that the addition of waste cassava and protected soybean meal increased (P<0.05) nutrient consumption (DM, OM, CP, CF, EE, and TDN). Milk production in the treatment group was higher (P<0.05) than control group (9.46 vs. 6.07 Ls/head/day). Milk protein production in the treatment group was higher (P<0.05) than control group (0.37 vs. 0.21 L/head/day). The content of milk protein and milk fat between the control and treatment groups was not significantly different (respectively 3.19 Vs 3.28; 4.46 vs 4.42 %). Milk protein and fat production in the treatment group was higher (P<0.05) than control group (0.21 vs 0.37; and 0.50 vs. 0.30 L/head/day respectively). The composition of glucose and blood urea in control and treatment dairy cows were not significantly different. In conclusion, giving cassava pomace and protected soybean meal to dairy cows during mid lactation increased nutrient consumption, milk production, milk protein production, and milk fat production but did not increase percentage of milk protein and fat. The treatment also did not affect blood glucose and nitrogen urea contents. 
The effect of protected soybean meal as a protein supplement on blood metabolites of lactating dairy cows Henny Leondro; Budi Prasetyo Widyobroto; Adiarto Adiarto; Ali Agus
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 29, No 2 (2019): Agustus
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2019.029.02.09

Abstract

The objective of this study was to determine the effect of undegradable dietary protein (UDP) using protected soybean meal supplementation on blood metabolites of lactating dairy cows. Eighteen early lactating Friesian Holstein cows of 1 to 3 years old were used in this study and observed twelve weeks. All cows received forage and concentrate in a ratio of 62:38 (DM basis). Adaptation to the diet was conducted for 4 weeks. The  cows were divided into 3 groups with different dietary treatments: control diet without UDP and mineral mix (T0), control diet + UDP 40 g/L milk + mineral mix (T1), and  control diet + UDP 60 g/L milk + mineral mix (T2). Fresh water was also offered ad libitum in the pen. In the days 14 and 7 pre-treatment, and in the days 1, 7, 14 and 21 post-treatment, cows were weighted and their blood were collected for chemical analysis. Means between treatments were analyzed using one-way analysis of variance (ANOVA). The results obtained from this study revealed that blood metabolites pattern of lactating cows before and after UDP supplementation subjects to considerable varia-tions. The UDP supplementation had a significant effect on the concentration of Ca. For comprehensive assessment of the effect of UDP supplementation on blood metabolites at different stages of parturient period, further detailed studies should be performed
Co-Authors Achadiah Rachmawati Adiarto Adiarto Adiarto Adiarto Ainin Fauzyah Aji, Riyan Nugroho Akhmad Fathoni Ali Agus Ali Agus Ali Agus Ali Agus Ali Agus Ali Agus Ali Agus Ambar Pertiwiningrum Anam, Moh Sofiul Andriyani Astuti Bambang Suhartanto Basofi Sabastian Budi Guntoro Canadianti, Monica Chusnul Hanim Diah Tri Widayati Dwi Astuti Endang Baliarti Gunawan Gunawan I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria Indratiningsih Indratiningsih Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Kustantinah Kustantinah Laelatul Rahmah Leondro, Henny Lies Mira Yusiati Lilis Hartati Mohamad Soejono Muhammad Nur Cahyanto Mulatmi, Septi Nur Wulan Nafiatul Umami Priyadi, Dwi Ahmad Rahma Fitriastuti Risa Antari Ristanto Utomo Rochijan Rochijan Shannora Yuliasari Sigit Bintara Sudi Nurtini Sudi Nurtini Sulvia Dwi Astuti Tety Hartatik Tridjoko Wisnu Murti Tyas Utami Yudi Adinata Yustina Yuni Surandiyah Zaenal Bachruddin