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All Journal HAYATI Journal of Biosciences Hemera Zoa Jurnal Peternakan Indonesia Jurnal Pendidikan Jasmani Kesehatan dan Rekreasi (Penjaskesrek) ARSHI Veterinary Letters Jurnal Ilmiah Fillia Cendekia Wahana Peternakan Hanoman Journal: Physical Education and Sport JUMORA: Jurnal Moderasi Olahraga Jurnal Pendidikan Indonesia (Japendi) JAS (Journal of Animal Science) Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Paradigma: Jurnal Filsafat, Sains, Teknologi, dan Sosial Budaya
Dwi Budiono
Study Program Of Veterinary Paramedic, College Of Vocational Studies, IPB University, Bogor, Indonesia
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Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium Budiariati, Vista; Budiono, Dwi; Fahrudin, Mokhamad; Juliandi, Berry; Rinendyaputri, Ratih; Boediono, Arief
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.143 KB) | DOI: 10.4308/hjb.27.2.89

Abstract

Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst.
Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium Vista Budiariati; Dwi Budiono; Mokhamad Fahrudin; Berry Juliandi; Ratih Rinendyaputri; Arief Boediono
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.143 KB) | DOI: 10.4308/hjb.27.2.89

Abstract

Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst.
Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) Noer Muhammad Dliyaul Haq; Diah Pristihadi; Vista Budiariati; Dwi Budiono; Al Mukhlas Fikri; Mokhamad Fahrudin; Cece Sumantri; Arief Boediono
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.185

Abstract

Interspecies embryo transfer is a seldom-used method to increase the successful conservation of endangered species. The study aimed to determine the potential development of interspecies reconstructed embryos. The present study used two animal models, Campbell hamsters (Phodopus campbelli) and mice (Mus musculus). The isolated inner cell mass (ICM) of hamster embryos were injected into the mice embryos. The embryos were transferred to the pseudopregnant mice using non-surgery embryos transfer methods. The fetuses were collected at day 13.5 of gestation for morphometric measurement and cytochrome b (Cyt b) analysis which used to determine the species of obtained fetuses. The results showed that the viability, pregnancy rate, and embryonic implantation ability of the interpecies reconstructed embryos did not differ significantly (p>0.05) compared to non-reconstructed embryos. Morphometric measurement showed that the crown-rump (CR) and the weight of fetuses in the reconstructed group were significantly higher than non-reconstructed group (p<0.05). According to Cytb analysis, the species of obtained fetuses were mice, while the population of hamster cells were found only in the blighted ovum (resorption). Therefore, it can be concluded that interspecies reconstructed embryos are able to implant. However, the population of mice cells are only found to develop.
PCS-1 Development of Mouse Parthenogenetic Embryos in Phosphate Free Medium Vista Budiariati; Dwi Budiono; Mokhamad Fahrudin; Berry Juliandi; Ratih Rinendyaputri; Arief Boediono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (516.416 KB)

Abstract

Parthenogenesis is an artificial oocytes activation process without paternal contribution so that embryos will develop without fertilization [3]. The process of parthenogenesis as a reproductive strategy occurs in species of insect, pisces, or amphibian, which not require any implantation. Naturally, parthenogenesis is not common in mammals, but by understanding cellular mechanism during fertilization, it is possible to artificially activate mammalian oocytes.Blastocyst, derived from parthenogenesis, can be used for developmental study, embryo reconstruction, and one of potential source for pluripotent stem cells. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate.One of the component that has been predicted to inhibit parthenogenetic embryo development is phosphate. Haraguchi et al. (1996)    reported that phosphate caused a negative effect on in vitro culture of AKR/N mice fertilized embryos, removal of phosphate elements was significantly improved the blastocyst rate up to 42.6% [1]. The effects of phosphate also became an interesting finding in the study that reported mouse fertilized embryos could well developed in modified medium rat 1 cell embryo medium (MR1ECM) which not contained any phosphate [2].The effect of phosphate on in vitro culture of mouse parthenogenetic embryo has not been clear. The aim of this research was to analyze inhibitory effect caused by phosphate in the medium and compare the development pattern between parthenogenetic and fertilized embryos in order to reach optimal production of parthenogenetic blastocyst for further purposes.  
Identifikasi Penyebab Penyakit Kulit pada Domba di Peternakan Kandangku Bogor Erni Sulistiawati; Heryudianto Vibowo; Surya Kusuma Wijaya; Dwi Budiono; Tekad Urip Pambudi Sujarnoko
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 25, No 1 (2023): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.25.1.29-36.2023

Abstract

Penyakit kulit menjadi salah satu isu penting pada peternakan karena dapat menyebabkan kerugian ekonomi akibat dampak zoonosis, penurunan kualitas dan harga jual ternak domba. Penyebab penyakit kulit seperti dermatofit dan infestasi ektoparasit menjadi perhatian serius. Tujuan dari penelitian ini adalah untuk mendapatkan informasi penyebab penyakit kulit pada ternak domba yang baru didatangkan ke peternakan guna membantu peternak agar tidak terjadi kesalahan dalam mengenali jenis penyakit kulit sehingga dapat membantu dokter hewan dalam penentuan penanggulangan yang tepat. Penelitian dilakukan pada 10 ekor domba yang baru hadir pada lokasi peternakan Kandangku, Bubulak Bogor yang dilaksanakan pada tanggal 13 dan 23 September 2022. Teknik koleksi data primer diperoleh dari hasil pemeriksaan klinis dan pencatatan body condition score (BCS), hasil evaluasi mikroskopik dari sampel rambut dan kulit menggunakan metode kerokan kulit dan trichogram. Jika hasil mikroskopik menemukan spora jamur, selanjutnya dilakukan kultur dermatofit menggunakan Kruse Dermatophyte Test serta pemeriksaan sitologi kultur. Gejala klinis pada kedua penyebab penyakit kulit sangat serupa yakni alopesia, rambut rapuh, berketombe dan keropeng sedangkan rata-rata BCS sekitar 1.3. Hasil pemeriksaan kerokan kulit ditemukan ektoparasit Bovicola ovis pada dua ekor domba, hasil pemeriksaan trichogram berupa temuan spora jamur dermatofit ditemukan pada seluruh domba yang diteliti, sedangkan pemeriksaan lanjut kultur dermatofit menunjukkan hasil mikroskopik adanya bentuk hifa dan jenis konidia jamur non dermatofit yaitu Aspergillus sp. Pencegahan dan penanggulangan dini terhadap kedua penyebab penyakit kulit memerlukan perhatian untuk mencegah kerugian peternak diantaranya dengan peningkatan BCS melalui pemenuhan nutrisi hewan didukung dengan sanitasi.
Pengaruh Teknik Silase Pakan Komplit Tersuplementasi Ampas Tahu Berprotein Kasar Berlebih pada Domba Secara InVivo Tekad Urip Pambudi Sujarnoko; dwi budiono; Dudi Firmansyah; Tenti Rahmawati
Jurnal Ilmiah Fillia Cendekia Vol 8 No 1 (2023): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v8i1.3420

Abstract

Tofu dregs are feed ingredients with high nutritional content and easy to rot. Therefore a silage storage technique is needed to preserve and reduce environmental pollution. The process of preserving high-protein feed by silage has a weakness in the process of lowering the pH. This happens because the ammonia from the deamination process is alkaline . The silage processing can converting some nutrients into organic acids which can improve livestock health and production. The Complete feed silage which was supplemented with tofu dregs was carried out on thin-tailed local rams aged 8-10 months and body weight 16-18 kg. The rams were grouped to 4 groups. The first gorup was P0 = complete feed which was suplemented with tofu dregs without silage processing compared to P1 = P0 + silage processing which was fermented for at least 14 days. The data then analyzed using t-independent method in a randomized block design on the basis of body weight. The results of the study showed that the average dry matter consumption of livestock was not significantly different P>0.1, the increase in monthly weight was higher in group P1 with a P value <0.05, the silage process increased the efficiency of p<0.05. Both treatment had no effect on cholesterol, blood urea nitrogen (BUN), glucose, red blood grains and white blood grains. So it can be concluded that feeding by silage is better than fresh . Keywords: Silage, rams, tofu dregs
PENGARUH POLA LATIHAN SIRKUIT TRAINING DAN LARI FARTLEK TERHADAP DAYA TAHAN AEROBIK ATLET PENCAK SILAT PAGAR NUSA Setyawan, Trinovandhi; Adi Pawitra, Paulus Rah Adi; Yahya, Alfian; Budiono, Dwi
Jurnal Pendidikan Jasmani Kesehatan dan Rekreasi (Penjaskesrek) Vol 11, No 2 (2024)
Publisher : STKIP Melawi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46368/jpjkr.v11i2.2469

Abstract

Abstract: The purpose of this study was to determine the effect of circuit training and fartlek running on increasing the aerobic endurance of Pagar Nusa Pencak Silat athletes in Batu City. The results of the study are as follows, the results of the pre-test of the training circuit have the highest score of 40.5 and the lowest score of 21.8 with an average of 33.34 with a standard deviation of 6.67. While the fartlek run has the highest score of 34.3 and the lowest score of 27.9, the average data is 30.70 with a standard deviation of 2.36. The results of the post-test of the training circuit have the highest score of 44.5 and the lowest score of 28.3 with an average of 37.31 with a standard deviation of 5.83. Meanwhile, the fartlek run has the highest score of 37.8 and the lowest score of 30.2, with an average data of 34.35 with a standard deviation of 2.41. So that it can be seen that there is no significant difference in the effect between circuit training and flex running. Researchers provide suggestions. This research still needs to be further developed in the hope of providing more significant data as a comparison between circuit training treatment and fartlek running by adding more research subjects. With the addition of research subjects, it is expected to get more different data and get more significant differences in the resultsKeywords: Circuit Training, Fartlek Running, Aerobic Endurance  Abstrak: Tujuan penelitian ini adalah untuk mengetahui pengaruh latihan sirkuit dan lari fartrek terhadap peningkatan daya tahan aerobik atlet Pencak Silat Pagar Nusa Kota Batu. Berikut temuannya: Nilai tertinggi sebesar 40,5, nilai terendah sebesar 21,8, dan mean standar deviasi sebesar 6,67. Sedangkan nilai tertinggi Fartlek Lari sebesar 34,3, nilai terendah sebesar 27,9, rata-rata data sebesar 30,70, dan  standar deviasi sebesar 2,36. Setelah dilakukan pengujian rangkaian latihan diperoleh nilai tertinggi sebesar 44,5, nilai terendah sebesar 28,3, nilai mean sebesar 37,31, dan  standar deviasi sebesar 5,83. Sedangkan nilai tertinggi Fartlek Lari sebesar 37,8, nilai terendah sebesar 30,2, rata-rata data sebesar 34,35, dan  standar deviasi sebesar 2,41. Dengan kata lain, tampaknya tidak ada banyak perbedaan dalam efek latihan sirkuit dan lari cepat.  Peneliti memberikan saran. Penelitian ini  perlu  dikembangkan lebih lanjut dengan memasukkan lebih banyak topik penelitian dengan harapan dapat memberikan data yang lebih bermakna sebagai perbandingan antara latihan sirkuit  dan lari fartlek. Dengan menambahkan tema penelitian, kita berharap memperoleh data yang lebih beragam dan  hasil yang berdampak pada varians yang lebih besar.Kata Kunci: Sirkuit Training, Lari Fartlek, Daya Tahan Aerobik
Study on Sheep Animal Welfare in Traditional Farmer, Located in Agro Edutourism Mulyaharja Bogor Regency Astini, Wining; Endah Anggraeni, Henny; Barunawati Siagian, Tetty; Sulistiawati, Erni; Vibowo, Heryudianto; Wijaya Kusuma, Surya; Budiono, Dwi; Fardesiana Putri, Miranti; Zahidah, Siti; Haikal, Muhammad; Faisal, Agus
JAS Vol. 10 No. 2 (2025): Journal of Animal Science (JAS) - April 2025
Publisher : Program Studi Peternakan, Fakultas Pertanian, Sains dan Kesehatan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Indonesian farmers do not understand about the meaning of animal welfare. It makes to be difficult to implement the animal welfare. It also impacts to the production and reproduction of the animal. On the other side, the sheep livestock in Mulyaharja are maintained traditionally. This study aims to evaluate the implementation of animal welfare principle in traditional sheep farming  in Mulyaharja subdistrict. This study used purposively sampling. The data analysis method is descriptive by processing data from interviews. The Animal Needs Index were used in this study. This study highlights significant shortcomings in sheep welfare under traditional farming practices in Mulyaharja Subdistrict with average ANI index 16.49. Targeted interventions in comfort of the lie down, number days of outside/year, pasture months/year, comfort of flooring, cleanliness sheep and pen, slipperiness, ease of passage, quality of light, quality of air and air flow, ind exposure in the lying area, condition of integument and animal health.
Blood cholesterol and glucose levels of sheep after being given feed supplemented with Pluchea indica leaf flour Budiono , Dwi; Sujarnoko , Tekad Urip Pambudi; Sulistiawati , Erni; Wijaya , Surya Kusuma; Sholeha , Novia Amalia; Sukmana , Raditya Teja
ARSHI Veterinary Letters Vol. 9 No. 1 (2025): ARSHI Veterinary Letters - February 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.1.25-26

Abstract

Domba merupakan salah satu jenis ternak yang umum dikonsumsi oleh penduduk. Daging domba mengandung kolesterol yang tinggi sehingga dapat menyebabkan hiperkolesterolemia pada individu yang mengonsumsinya. Tujuan dari penelitian ini adalah untuk mengkaji dampak pemberian Pluchea indica terhadap kadar kolesterol dan glukosa darah pada domba. Sebanyak 15 ekor domba dibagi menjadi 4 kelompok perlakuan yang berbeda. Kelompok-kelompok tersebut dikategorikan menurut konsentrasi tepung P. indica dalam konsentrat, yaitu 0%, 2%, 4%, dan 8%. Hewan-hewan tersebut menjalani terapi makan selama 35 hari. Hasil penelitian menunjukkan bahwa penambahan tepung P. indica 4% dalam konsentrat menghasilkan penurunan kadar kolesterol darah pada domba yang signifikan (p<0,05). Tidak terdapat perbedaan kadar glukosa yang signifikan antar kelompok (p>0,05). Penelitian ini menyimpulkan bahwa penambahan tepung P. indica 4% ke dalam konsentrat merupakan formulasi yang paling efektif untuk menurunkan kadar kolesterol darah.
PERMASALAHAN INFESTASI EKTOPARASIT PADA PETERNAKAN DI KELURAHAN MULYAHARJA : Ectoparasite Infestation Problems in Livestock in Mulyaharja Subdistrict Tetty Barunawati Siagian; Henny Endah Anggraeny; Heryudianto Vibowo; Erni Sulistiawati; Surya Kusuma Wijaya; Dwi Budiono; Miranti Fardesiana Putri; Wining Astini; Muh Rifky Rachman; Kukuh Amru Dhiya Rahmat; Agus Faisal
Wahana Peternakan Vol. 9 No. 2 (2025): Wahana Peternakan
Publisher : Faculty of Animal Science, University of Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v9i2.1829

Abstract

Mulyaharja merupakan salah satu kelurahan yang terdapat di Kota Bogor. Pekerjaan masyarakatnya sebagai petani dan juga peternak. Beternak domba dan kambing dilakukan secara tradisional tanpa memiliki pengetahuan. Permasalahan dihadapi peternak di Kelurahan Mulyaharja yaitu infestasi ektoparasit. Tujuan dari penelitian yaitu mengidentifikasi infestasi ektoparasit pada domba dan kambing pada peternakan rakyat di Kelurahan Mulyaharja. Penelitian menggunakan 48 ekor domba dan 14 ekor kambing. Pemeriksaan ektoparasit dilakukan secara natif. Hasil pemeriksaan menunjukkan ditemukannya kutu Bovicola ovis, dan tungau Sarcoptes scabiei. Kutu Bovicola ovis ditemukan pada domba dan kambing, sedangkan infestasi Sarcoptes scabiei hanya ditemukan pada satu ekor kambing. Bovicola ovis merupakan kutu pengunyah dan pengigit pada domba namun dapat menginfestasi kambing. Penyebarannya sangat cepat dalam satu kandang. Sarcoptes scabiei merupakan tungau yang hidup dibawah kulit dan membentuk terowongan. Tungau Sarcoptes scabiei  bersifat zoonosis. Infestasi ektoparasit merupakan salah satu permasalahan peternakan di Kelurahan Mulyaharja.   Kata kunci: Domba, Damalinia ovis, kambing, Oribatid mite, Sarcoptes scabiei,
Co-Authors Achmad Afandi, Achmad Adi Pawitra, Paulus Rah Adi Agus Faisal Al Mukhlas Fikri Amaq Fadholly Anggraeni, Henny Endah Anggraeni, Henny Endah Anisa Rahma, Anisa Arief Boediono Aris, Tria Muhammad Artanty, Ary astini, wining Berry Juliandi Budiariati, Vista Calista, Pradnya Catur Mardianto, Paulus Cece Sumantri Diah Nugrahani Pristihadi Dian Fitri Argarini, Dian Fitri Dudi Firmansyah Elfi Anis Saati Erni Sulistiawati Faisal, Agus Fardesiana Putri, Miranti Henny Endah Anggraeny Herlambang, Alexander Heryudianto Vibowo ILHAM HABIBI, AHMAD Kukuh Amru Dhiya Rahmat Mika Ambarawati, Mika Miranti Fardesiana Putri MISTIANAH, MISTIANAH Mokhamad Fahrudin Muh Rifky Rachman Muhammad Haikal Muhammad Haikal Adhim Mushofi, Yuskhil Noer Muhammad Dliyaul Haq Nugraha, Ivan Taufiq Putri, Aneta Dwi Rahma Rahmat, Kukuh Amru Dhiya Ratih Rinendyaputri Ratih Rinendyaputri Ratih Rinendyaputri Setyawan, Trinovandhi Sholeha , Novia Amalia Siagian, Tetty Barunawati Siti Zahidah Siti Zahidah Sukmana , Raditya Teja Surya Kusuma Wijaya Surya Kusuma Wijaya Tekad Urip Pambudi Sujarnoko Tenti Rahmawati Trio Ageng Prayitno, Trio Ageng Utomo, Irfan Hakim Wijaya , Surya Kusuma Wijaya Kusuma, Surya wining astini Yahya, Alfian Zonathan, Hendri