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KARAKTERISASI FTIR DAN UJI TOKSISITAS HIDROKSIAPATIT NANOKRISTALIN Corbicula moltkiana TERHADAP SEL HUMAN DERMAL FIBROBLAS ADULT Al Majiid, Muhammad Shalahuddin; Pascawinata, Andries; Lestari, Citra
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Vol 12, No 1 (2025): Vol 12 No 1 (Juni 2025)
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/jbd.v12i1.1814

Abstract

Background: One way to reconstruct bones is with bone grafting. Hydroxyapatite (HA) is often used as a bone graft, because it has biocompatibility, osteoconductive, bioactive, non-toxic and non-immunogenic properties. HA can be obtained from natural materials such as shells, limestone, egg shells and others. Using natural materials has several advantages such as high Ca/P ratio content, abundant quantities, and cheaper material production costs. The nanocrystalline hydroxyapatite (nHA) used in this research is Corbicula moltkiana shell powder. Purpose: to know; a) FTIR characterization of nHA Corbicula moltkiana; b) the level of toxicity of Corbicula moltkiana nHA on HDFA cells, and c) the ideal concentration of Corbicula moltkiana nHA on HDFA cell viability. Methods: in vitro laboratory experiment with post-test only control group design. To find out the characterization, use the FTIR characterization test. Toxicity test using the MTT method. The research samples were Corbicula moltkiana nHA powder and HDFA cells. The samples were divided into 4 groups, namely the control cell group and 3 groups of nHA Corbicula moltkiana with concentrations of 25 µg/ml, 50 µg/ml, and 100 µg/ml. Results: 1) Corbicula moltkiana nHA powder has good HA quality, 2) cell viability at concentrations of 25 µg/ml, 50 µg/ml, and 100 µg/ml against HDFA cells was 99.64%, 96.51% and 83.93%, respectively. Conclusions: 1) the higher the concentration of Corbicula moltkiana nHA, the lower the viability of HDFA cells, but all concentrations studied were not toxic, 2) Corbicula moltkiana nHA is biocompatible with HDFA cells.
Pemberian Topikal Fluor pada Siswa SDN 10 Sungai Sapih Padang Anggraini, Netta; Lestari, Citra; Zia, Hanim Khalida; Ningrum, Valendriyani; Lisfrizal, Hamdy
Jurnal Pengabdian Masyarakat Kesehatan Gigi FOKGII (JPMKG FOKGII) Vol. 1 No. 2 (2024): Agustus 2024
Publisher : Forum Komunikasi Kedokteran Gigi Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kesehatan gigi mulut sangat perlu diperhatikan sejak dini, Karies merupakan penyakit yang banyak menyerang anak – anak terutama umur 6 sampai 9 tahun. Anak- anak perlu melakukan Pemeliharaan kesehatan gigi dan mulut secara baik Pencegahan karies gigi bertujuan untuk mempertinggi taraf hidup dan memperpanjang kegunaan gigi didalam mulut. Kesehatan gigi sulung pada anak akan menjadi penentu kondisi gigi permanen yang akan menggantikannya. Masalah kesehatan gigi dan mulut yang paling umum adalah karies gigi. Data Riset Capaian Kesehatan Dasar 2018 menunjukkan prevalensi karies di Indonesia adalah 57,6%. Ada tiga macam pencegahan yang dapat dilakukan untuk mencegah karies yaitu pencegahan primer, sekunder, dan tersier. Tahap primer pencegahan karies meliputi pendidikan kesehatan gigi, pemeliharaan kesehatan gigi, pemeriksaan gigi secara teratur, pemberian fissure sealant, dan pemberian topikal application fluoride (TAF).). Topikal aplikasi fluoride adalah salah satu cara yang paling efektif untuk mencegah karies. Kegitaan ini dilaksanakan 10 Sungai Sapih.
PENGARUH EKSTRAK KULIT PISANG AMBON (MUSA PARADISIACA L) TERHADAP JUMLAH SEL INFLAMASI PADA TIKUS PERIODONTITIS Orienty, Fauzia Nilam; Lestari, Citra; Andriani, Ika
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Vol 10, No 2 (2023): Vol 10 No 2, Desember 2023
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/jbd.v10i2.1493

Abstract

Introduction: Periodontitis is a chronic infection of periodontal tissues caused by interplay between periodontopatogen bacterial and the immune system. In periodontitis condition, inflammatory cells are found in large numbers and will release proinflammatory cytokines. Non-steroidal anti-inflammatory (NSAID) is one of a class of drugs used in periodontitis cases treatment. However, long treatment using NSAIDs may give side effects such as gastrointestinal disorder. Ambon banana peel (Musa paradisiaca L),a medicinal plant contains flavanoid, alkonoid, tanin and saponin which has anti-inflammatory effect due to its ability in inhibiting inflammatory cell migration. This research aimed to study the effect of the Ambon banana peel extract as an antiinflammation on the number of inflammatory cells infiltration in the periodontitis induced rats. Methods : This research uses experimental laboratory with Post Test Only Control Group Design. Silk ligature was placed in subgingival areas at the lower anterior teeth of male Wistar rats for 14 days to induce periodontitis. After 14 days, the ligation was released and the rats from each group were administered orally either with 25%, 50% concentrations extract and aquades (control negative). The rats were then decapitated 1, 5 and 7 days after the treatment.The serially sections were stained with hematoxylin eosin to examine the number of inflammatory cell infiltration. Results :Two-way Anova showed significant differences (p <0.05) among groups, indicating that Ambon banana peel extract affected the number of neutrofil cells infiltration. Conclusion: There is an effect of Ambon banana peel (Musa paradisiaca L) extract to reduce inflammatory cells infiltration.
PENGARUH TEKNIK PENYIKATAN GIGI METODE BASS DENGAN TIPE BULU SIKAT YANG BERBEDA TERHADAP INDEKS PLAK Utari, Rahmi; Lestari, Citra
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Volume 1, Nomor 2, Desember 2014
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (45.497 KB) | DOI: 10.33854/JBDjbd.29

Abstract

Plak gigi merupakan suatu lapisan lunak yang terdiri atas kumpulan mikroorganisme yang berkembang biak di atas suatu matriks yang terbentuk dan melekat erat pada permukaan gigi yang tidak dibersihkan. Metode penyikatan gigi yang umum dilakukan untuk mengurangi plak yaitu dengan metode Bass, baik dengan tipe bulu sikat yang lurus dan bersilang. Tujuan dari penelitian ini adalah untuk mengetahui hubungan penyikatan gigi dengan tipe bulu sikat yang berbeda secara metode bass terhadap indeks plak. Jenis penelitian yang digunakan adalah penelitian eksperimental. Plak diukur dengan menggunakan indeks plak modifikasi Quigley & Hein sebelum dan sesudah penyikatan gigi. Analisis data menggunakan uji t. Hasil penelitian didapatkan p=0,012 (0.05), menunjukkan adanya perbedaan yang signifikan antara penyikatan dengan bulu sikat yang lurus dan bersilang tetapi dapat disimpulkan bahwa dengan sikat gigi yang lurus plak sudah dapat berkurang.