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All Journal JURNAL GIZI INDONESIA Jurnal Kedokteran Syiah Kuala Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Jurnal Ilmiah Mahasiswa Kedokteran Medisia Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Indian Journal of Forensic Medicine & Toxicology Narra J Jurnal Mahasiswa Karakter Bangsa Media Farmasi Indonesia Jurnal Kedokteran Hewan Trends in Infection and Global Health
Hanifah Yusuf
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Religion Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Global prevalence of persistent neuromuscular symptoms and the possible pathomechanisms in COVID-19 recovered individuals: A systematic review and meta-analysis Fajar, Jhonny K.; Ilmawan, Muhammad; Mamada, Sukamto; Mutiawati, Endang; Husnah, Milda; Yusuf, Hanifah; Nainu, Firzan; Sirinam, Salin; Keam, Synat; Ophinni, Youdiil; Rosiello, Francesco; Fahriani, Marhami; Rosa, Sandro GV.
Narra J Vol. 1 No. 3 (2021): December 2021
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v1i3.48

Abstract

This study was conducted to determine the prevalence of prolonged neuromuscular symptoms, including fatigue, anosmia, headache, myalgia, and joint pain in COVID-19 survivors hospitalized with mild, moderate, or severe infections worldwide. The search was conducted up to January 30th, 2021 using three databases (PubMed, Scopus, and Web of Science) to identify potentially eligible studies. Data on study characteristics, follow-up characteristics, and severity of COVID-19 during hospitalization were collected in accordance with PRISMA guidelines. The Newcastle-Ottawa scale was used to assess the quality of relevant articles. The estimated prevalence of specific prolonged neuromuscular symptoms and the association between COVID-19 severity and occurrence of prolonged neuromuscular symptoms was analyzed wherever appropriate. Database search yielded 4,050 articles and 22 articles were included for meta-analysis. The estimated prevalence of prolonged fatigue was recorded in 21.2% (95%CI: 11.9%–34.8%) of 3,730 COVID-19 survivors. Persistent anosmia was recorded in 239 of 2,600 COVID-19 survivors (9.7%, 95%CI: 6.1%–15.2%). In 84 out of 2,412 COVID-19 survivors (8.9%, 95%CI: 3.2%–22.6%), prolonged headache was observed. A total of 53 out of 1,125 COVID-19 patients (5.6%, 95%CI: 2.1%–14.2%) complained of persistent myalgia even after being discharged from the hospital. The prevalence of prolonged joint pain was in 15.4% (95%CI: 8.2%–27.2%) of subjects. Due to data scarcity on COVID-19 severity and prolonged neuromuscular symptoms, association analysis could not be conducted. Widespread concern regarding long-term impacts of COVID-19 was raised after several studies reported prolonged symptoms in COVID-19 survivors. Numerous theories have been proposed to address this concern; however, as the research on this pandemic is still ongoing, no explanation is definitive yet. Therefore, follow-up studies in COVID-19 survivors after recovery from COVID-19 are warranted to determine the pathogenesis of prolonged symptoms. PROSPERO registration: CRD42021242332.
Cytotoxic activity of ethyl acetate extract of Chromolaena odorata on MCF7 and T47D breast cancer cells Yusuf, Hanifah; Novia, Hijra; Fahriani, Marhami
Narra J Vol. 3 No. 3 (2023): December 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i3.326

Abstract

One of the most cancers in women that can be fatal is breast cancer. Radiation therapy, chemotherapy, or a combination of the two are often used to treat cancer, and these treatments tend to modify the immune system and weaken defences. Using natural compounds from plants has become a research interest to prevent cancer cell development. The aim of this study was to determine the anticancer activities of ethyl acetate extract of Chromolaena odorata (EACO) against breast cancer cells (MCF-7 and T47D). The viability of the cells was determined by the MTT colorimetry assays. The apoptosis test was performed by using flow cytometry. The IC50 value for MCF-7 cells was 218.78 μg/mL and 307.61 μg/mL for T47D. The extract acted selectively against breast cancer cells, with selectivity indexes against MCF-7 and T47D were 12.77 and 9.08, respectively. The viable cells of T47D cells were decreased from 85±36.5% (24 hours) to 54±34% (48 hours) after treatment with IC50 of EACO. Significant decrease of the MCF-7's viable cells were observed between 48 and 72 hours after treatment with IC50 (68.5±17.7% to 51.01±12.1%, respectively). Apoptosis assay showed that T47D and MCF-7 cells were mainly in the necrosis stage (83.35±0.49% and 95.15±1.76%, respectively). This study suggested that ethyl acetate extract of C. odorata is promising to be developed as an anticancer agent.
PEMAHAMAN MATERI KETAHANAN NASIONAL TERHADAP KARAKTER DISIPLIN SISWA KELAS X DI SMA PGRI 56 CIPUTAT Yusuf, Hanifah
Jurnal Mahasiswa Karakter Bangsa Vol. 1 No. 1 (2021): Jurnal Mahasiswa Karakter Bangsa Vol.1 No.1 Maret 2021
Publisher : Universitas Pamulang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Setiap siswa masih kurang memahami materi tentang ketahanan nasional sehingga siswa menjadi kurang paham dengan maksimal dalam belajar. Adapun tujuan dari materi ini agar dapat menumbuhkan sikap tanggung jawab siswa dan karakter yang baik. Penelitian ini memiliki tujuan Untuk mengetahui pengaruh Pemahaman Materi Ketahanan Nasional terhadap Karakter Disiplin Siswa kelas X di SMA PGRI 56 Ciputat. Peneliti menggunakan metode penelitian kuantitatif asosiatif yang populasinya ialah  seluruh siswa SMA PGRI 56 Ciputat yang berjumlah 247 peserta didik dan populasi terjangkaunya yaitu peserta didik kelas X yang berjumlah 81 dengan 68 peserta didik yang dijadikan sebagai sampel.Penelitian dengan menggunakan uji t yang telah dilakukan menunjukkan data thitung = 12,04 dan ttabel = 0,26 dengan kriteria pengujian Jika thitung< ttabel maka H0 diterima dan H1 ditolak dan jika thitung ≥ttabel maka H0 ditolak dan H1 diterima. Dengan demikian maka H0 ditolak dan H1 diterima karena thitung> ttabel hal ini berarti Terdapat Pengaruh Yang Signifikan Antara Pemahaman Materi Ketahanan Nasional terhadap Karakter Disiplin Siswa Kelas X di SMA PGRI 56 Ciputat Kata kunci : Pemahaman Materi Ketahanan Nasional, Karakter Disiplin Siswa 
Analisis Nilai Anthropometri Dan Malnutrisi Pada Laki-Laki Dan Perempuan Usia 17-22 Tahun: Suatu Studi Cross-Sectional Yusni, Yusni; Yusuf, Hanifah; Murzalina, Cut; Rahman, Safrizal
Majalah Kedokteran Indonesia Vol 74 No 6 (2024): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.74.6-2024-928

Abstract

Introduction: Malnutrition is a worldwide problem that has yet to be resolved, and its prevalence in Indonesia has increased in tandem with changes in people’s lifestyles. Method: This study aims to determine nutritional status based on anthropometric values and investigate malnutrition in male and female aged 17–22 years. The research was conducted using a cross-sectional study. The subjects were male and female, aged 17–22 years, and healthy, with a total of 1,669 male (n=210) and female (n=1459). A questionnaire was used to obtain data. The data was analyzed using a chi-square test (p less than 0.05) and an independent sample t-test (p less than 0.05). Result: Malnutrition was found in subjects who were underweight (male=28.10% and female=26.39%), overweight (male=8.10% and female=9.32%), and obese (male=8.57% and female=6.85%). Total malnutrition in male and female was 27.25% underweight, 8.71% overweight, and 7.71% obese. Nutritional status was not related to gender (p greater than 0.05): underweight (p=0.74), normo-weight (p=0.48), overweight (p=0.34), and obesity (p=0.74). Conclusion: The double burden of malnutrition in males and females aged 17-22 years in Banda Aceh, Aceh, Indonesia is undernutrition and overnutrition, cases of undernutrition were more numerous than overweight and obesity, even though the number of malnutrition cases did not show any difference between men and women in all categories.
Effectiveness of Chromolaena odorata L. cream in reducing melanocyte cell count and TNF- levels in male Wistar rats exposed to UVB radiation Sari, Maya; Yusuf, Hanifah
Trends in Infection and Global Health Vol 5, No 1 (2025): June 2025
Publisher : School of Medicine, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/tigh.v5i1.47237

Abstract

Chromolaena odorata L. is a plant traditionally used in herbal medicine. The leaves of C. odorata contain bioactive compounds with high antioxidant activity, making them potential therapeutic agents for hyperpigmentation and inflammation. Ultraviolet (UV) radiation is an extrinsic factor and a major cause of skin aging, known as photoaging. Photoaging is a process due to chronic UV exposure that induces the formation of reactive oxygen species (ROS). Excess ROS, surpassing body's antioxidant capacity, leads to skin barrier damage, DNA damage, and hyperpigmentation. Photoprotection is essential to mitigate these effects. The aim of this study was to evaluate the antioxidant activity of C. odorata leaf ethanol extract cream and its effect on melanocyte cell and tumor necrosis factor alpha (TNF-) levels in male Wistar rats (Rattus norvegicus). A true experimental post-test with control group design was used, involving 42 male Wistar rats divided into seven groups: Group Ino UVB exposure, cream base only; group IIUVB exposure only; group IIIUVB exposure with cream base; group IVUVB exposure with 5% C. odorata ethanol extract cream; group VUVB exposure with 10% C. odorata ethanol extract cream; group VIUVB exposure with 2% hydroquinone cream; and group VIIUVB exposure with 5% vitamin C cream. C. odorata extract was formulated into a cream and tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The cream was topically applied to rats dorsal skin irradiated with UVB for three weeks following a scheduled exposure protocol. At weeks 3 and 6, three rats per group were euthanized using cervical dislocation, and 55 cm dorsal skin samples were collected for histological analysis of melanocyte count and TNF- levels via enzyme-linked immunosorbent assays (ELISA). The results showed that C. odorata ethanol extract cream contained strong antioxidants with an IC50 of 57.7 mg/L. Statistical analysis revealed significant differences in melanocyte count and TNF- levels among groups at weeks 3 and 6 (p0.05). The 10% C. odorata extract cream had the lowest melanocyte counts, while the 5% C. odorata extract cream exhibited the lowest TNF- levels, indicating the extracts photoprotective and anti-inflammatory effects.
A CLOSE POSITIVE ASSOCIATION BETWEEN OBESITY AND BLOOD PRESSURE IN RATS Yusni, Yusni; Yusuf, Hanifah
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i1.23913

Abstract

The purpose of this study was to analyze how the effect of each increase in Body Mass Index (BMI) on the increase in blood pressure (BP). The research design was a laboratory experiment with 52 non-obese and obese rats as subjects (non-obese, n=14 and obese, n=38). Rats were induced obesity using a high-fat diet for four weeks. BMI used is based on the Lee Index with obesity indicators if 300 g/cm. Data analysis using independent sample t-test, simple linear regression test, and Pearson correlation test; (p0.05). The results: BP in non-obese rats was lower than obese rats (89.007.38 vs 190.114.42; p=0.001*), BMI was positively associated with BP (p=0.001*); an increase in BMI will increase BP (p=0.001*), there is a strong correlation between BMI and BP (r=0.977; p=0.001*). The regression equation (Y = 707.42 + 2.75 X) indicates that every 1 g/cm increase in BMI will be followed by an increase in BP of 2.75 mmHg. The analysis of R-square = 0.942 shows that the accuracy of the linear equation model is 94.2%. In conclusion, BMI has a strong positive correlation with BP, an increase in BMI will increase BP; obesity induces high blood pressure in rats.
ANTICANCER ACTIVITY OF ETHANOL EXTRACT OF YELLOW ROOT (Arcangelisia flava) ON HEPG2 HEPATOCELLULAR CANCER CELLS Yusuf, Hanifah; Fahriani, Marhami; Murzalina, Cut
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i1.23615

Abstract

This study aimed to evaluate anticancer activity and apoptosis induction of ethanolic extract of Arcalengisia flava (AF) roots on HepG2 cancer cell lines. The AF roots were extracted by maceration using ethanol 80%. MTT assay method was used to evaluate the anticancer activity and the proliferation of HepG2 cells. Flow cytometry method was used to assess the induction of HepG2 cells apoptosis. This study showed that the IC50 of AF ethanol extract against HepG2 cells was 109.14 g/ml. With IC50 treatment, the apoptosis assay showed a significant decrease in intact cells (80.101.7%) and a significant increase in early apoptosis (7.90.7%) and late apoptosis cells of HepG2 cancer cells (4.90.35%) compared to control cells. Moreover, the proliferation of HepG2 cells was declined significantly in 48 and 72 hours after treatment with IC50 (77.55.76% and 64.35.37%, respectively) and 2xIC50 of the extract (75.91.79% and 70.54.27%, respectively). This research suggests that the ethanolic extract of AF roots can potentially be used for hepatocarcinoma treatment.
GROWTH INHIBITION AND INDUCTION OF APOPTOSIS IN MCF-7 AND T47D BREAST CANCER CELL LINES BY ETHANOL EXTRACT OF SEURAPOH (Chromolaena odorata) LEAVES Yusuf, Hanifah; Kamarlis, Reno Keumalazia; Yusni, Yusni
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i3.17227

Abstract

This study aims to determine the growth inhibition and apoptosis induction of MCF-7 and T47D cancer cells by ethanol extract of Chromolaena odorata leaves. A post-test with control group design was used in this study. The extract was made by maceration with 80%ethanol and the tested concentrations used were 7.80 g/mL-500 g/mL with standard drug doxorubicin ranged from 1.56 g/mL-100 g/mL.The growth inhibition was determined by the MTT colorimetry method, apoptosis induction by double staining using acridine orange-ethidiumbromide, and the existence of apoptosis was proven immunocytochemically through the expression of Bcl-2 proteins. The results showed that the growth inhibition of MCF-7 was 100.29%-28.19% and T47D was 100.37%-16.01%. The IC50 values of MCF-7 was 327.34 g/mL and T47D was 135.16 g/mL. The presence of apoptosis was marked by finding the morphological change of cells such as dead, necrosis, and chromatin condensation cells. This suggests that interventions with ethanol extract of Chromolaena odorata leaves can induce apoptosis that has been proven by reducing the expression of Bcl-2 proteins.
Role of interleukin-2 in wound healing: A review study Handayani, Putri; Yusuf, Hanifah; Gholib, Gholib
Trends in Infection and Global Health Vol 3, No 2 (2023): December 2023
Publisher : School of Medicine, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/tigh.v3i2.35773

Abstract

A wound is a condition where tissue damage occurs due to disruption of tissue continuity caused by injury or surgery. Wound healing is a normal biological process in the human body which aims to restore the function and integrity of damaged tissue. In the process, wound healing involves cellular and biochemical responses, including antioxidants and cytokines, which occur locally and systemically. Interleukin-2 (IL-2) is a cytokine that plays a role in wound healing. It is produced by CD8+ and CD4+ T cells in an active state. In the wound healing process, IL-2 plays an important role in activating macrophages and natural killer (NK) cells and encouraging the proliferation of B cells and T cells. In this review, the role of the IL-2 in wound healing is discussed comprehensively.
Co-Authors . Maryatun . Sofia . Suryawati . Vera Dewi Mulia, Troef Soemarno Amida Jannatus Saleha Andri Frediansyah, Andri Andrian Saputra Buchari Buchari Cut Murzalina Dina Alia ENDANG MUTIAWATI, ENDANG Fahmiati Arifna Fahriani, Marhami Fajar, Jhonny K. Feliatra Firzan Nainu Gholib Gholib, Gholib Hijra Novia Suardi Husnah, Milda Ilmawan, Muhammad Iqmal Tahir Jonny K. Fajar Jufitriani Ismy Keam, Synat Mahardika Agus Wijayanti Maimun Syukri, Maimun Maliga, Helnida A. Mamada, Sukamto Masyeni, Sri Maya Sari Murzalina, Cut Mustofa Mustofa Novia, Hijra Ophinni, Youdiil Putri Handayani Ratna Asmah Susidarti Reno Keumalazia Kamarlis Rosa, Sandro GV. Rosiello, Francesco Safrizal Rahman, Safrizal Sari, Etika Sirinam, Salin Suryawati Suryawati Usman, Said Yusni Yusni Yusni Yusni Zahratul Aini