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All Journal Journal of Food and Pharmaceutical Science VALENSI JSFK (Jurnal Sains Farmasi & Klinis) Pharmaceutical And Biomedical Sciences Journal (PBSJ) Pharmaceutical Sciences and Research (PSR)
Zilhadia Zilhadia
Pharmacy Department Faculty Of Health Sciences Syarif Hidayatullah State Islamic University Jakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Journal : VALENSI

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Determination Of Formaldehyde In Tofu From Ciputat Traditional Market With Colorimetry Method Zilhadia Zilhadia; Sanny Susanti
Jurnal Kimia Valensi Jurnal Valensi Volume 2, No.2, Mei 2011
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.402 KB) | DOI: 10.15408/jkv.v2i2.204

Abstract

Tofu, a food product made from soybean, contain water and it is very easy to overgrown microbes.Based on a survey, many products of tofu contain formaldehyde as preservative. Formaldehyde is apreservative that the use is prohibited for food because it can cause cancer in humans. The aim of thisstudy was determined concentration of formaldehyde in tofu from traditional market Ciputat usingSpectrophotometer UVI-VIS. Steam distillation was used for sample extraction process. Distillate wasreacted with Nash reagent. The equation of calibration curve is y=0,0032x – 0,0079 and correlationcoefficient of the linear regression of 0,9992. The experimental results obtained LOD value of11.1328 μg/ml, LOQ value of 37,1094 μg/mL The precision of this analytical method were lower than2% for each of the sample, while method’s accuration for tofu was 98,69% ± 0,4085%. Results ofsample analysis from Ciputat market, samples were detected containing formaldehyde. Formaldehydeconcentrations are 104,87 μg/mL, 11,21 μg/mL, 1,96 μg/mL, 190,80 μg/mL, 201,98 μg/mL, 10,47μg/mL, and 3,31 μg/mL.
Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras Sandra Hermanto; Fahrur Rahman Saputra; Zilhadia .
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 1, No. 1, Mei 2015
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.816 KB) | DOI: 10.15408/jkv.v0i0.3150

Abstract

Gelatin as the main ingredient of capsules is still a problem for a moslem. Most of gelatin production remains largely derived from non-halal materials. One of gelatin source is came from collagen of the skin and bones of bovine or pork. The main of study is determine the source of gelatin used in hard capsules by using SDS-PAGE (Sodium Dodecyl Sulphate Gel electrophoresis Poly Acrylamide) method. In the early stages, optimization of standards bovine and pork gelatin were hydrolyzed by pepsin at pH 4.5 and 60°C for 1 hour, 2 hours, and 3 hours. Gelatin hydrolyzateswere analyzed by SDS-PAGE to determine the optimal hydrolysis time. Identification of gelatin hydrolyzate fragments were carried by molecular weight. Hydrolysis time optimization throught applied to identify the source of hard gelatin capsules in the samples obtained from market and compared with the simulation of hard gelatin capsules. The results showed there were of specific bands of bovine gelatin with a molecular weight of 11,4 kDa; 34 kDa; 47kDa and specific bands of pork gelatin with a molecular weight of 24.7 kDa; 28 kDa; and 60 kDa. Similar results were obtained on a sample of hard capsules with bands of protein fragments that were identical to bovine gelatinstandard. Based on the results,each of the samples were tested contain of bovine gelatin respectively. DOI :http://dx.doi.org/10.15408/jkv.v0i0.3150
Co-Authors Abdi Wira Septama Afifah Nurul Izzah Ayu Gustida Barita Juliano Siregar Bayyinah Bayyinah Bayyinah, Bayyinah CHRIS ADHIYANTO CHRIS ADHIYANTO Fahrur Rahman Saputra Farida Kusumaningrum Fathmah Syafiqoh Gustida, Ayu H Herdini Khairunnisa, Nadiah Kusumaningrum, Farida Maulina Handayani Munfarikhatin, Atina Nadiah Khairunnisa Ofa Suzanti Betha Sabrina Dahlizar Salman Alfarisi Salman Alfarisi Sandra Hermanto Sanny Susanti Sofa Fajriah Supandi Supandi Supandi Supandi Supandi suryani, nelly Suzanti Betha, Ofa Syafiqoh, Fathmah Tengku Riza Zarzani N Yardi Yardi Yetika Alvionita Yuni Anggraeni