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All Journal Microbiology Indonesia WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Damianus Journal of Medicine Majalah Obat Tradisional Journal Of Vocational Health Studies Syntax Literate: Jurnal Ilmiah Indonesia Indonesian Journal of Biotechnology and Biodiversity The Indonesian Journal of Infectious Diseases Journals of Ners Community Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Biomedika dan Kesehatan EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi MAHESA : Malahayati Health Student Journal Multidiciplinary Output Research for Actual and International Issue (Morfai Journal) Cermin Dunia Kedokteran East Asian Journal of Multidisciplinary Research (EAJMR) Formosa Journal of Science and Technology (FJST) Jurnal Sehat Indonesia (JUSINDO) Journal of Clinical Microbiology and Infectious Diseases Jurnal Kedokteran Meditek Biomedika Cermin Dunia Kedokteran Biodiversitas Journal of Biological Diversity
Tjampakasari, Conny Riana
Departemen Mikrobiologi Fakultas Kedokteran Universitas Indonesia
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EVALUATION OF THE MICRODILUTION METHOD FOR DETERMINATION OF THE MINIMUM INHIBITORY CONCENTRATION (MIC) OF TERBINAFINE AGAINST TRICHOPHYTON RUBRUM Thalia Anggrea Noor; Delly Chipta Lestari; Conny Riana Tjampakasari
Multidiciplinary Output Research For Actual and International Issue (MORFAI) Vol. 6 No. 4 (2026): Multidiciplinary Output Research For Actual and International Issue
Publisher : RADJA PUBLIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.21912182

Abstract

Trichophyton rubrum is the leading cause of dermatophytosis with a global prevalence of 50–90%. Terbinafine is a first-line therapy that works by inhibiting the squalene epoxidase enzyme in ergosterol biosynthesis. Accurate determination of the minimum inhibitory concentration (MIC) using a standardized susceptibility test method is necessary to ensure the effectiveness of therapy. This study aims to standardize the concentration of terbinafine using the microdilution method based on CLSI M38-A2 guidelines against Trichophyton rubrum ATCC MYA-4438. The test was carried out using an inoculum concentration of 1×10³–3×10³ CFU/mL with varying terbinafine concentrations , then incubated at 28–30°C for 3–4 days. The results showed MIC values of 0.125 µg/mL; 0.25 µg/mL; and 0.125 µg/mL, respectively. An essential agreement (EA) value of 100% indicates high method precision. Morphological analysis revealed changes in hyphal structure in the form of fragmentation, a decrease in the number of conidia, cytoplasmic clumping, and uneven cell walls due to squalene accumulation. Thus, the microdilution method proved valid and reproducible for determining the MIC of terbinafine against T. rubrum .
Short Communication: Characterization of phenotypic and genotypic antibiotic resistance patterns in Escherichia coli isolates from bloodstream infections CONNY RIANA TJAMPAKASARI; LANY STEVINA; RAFIKA INDAH PARAMITA
Biodiversitas Journal of Biological Diversity Vol. 27 No. 3 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270320

Abstract

Abstract. Tjampakasari CR, Stevina L, Paramita RI. 2026. Short Communication: Characterization of phenotypic and genotypic antibiotic resistance patterns in Escherichia coli isolates from bloodstream infections. Biodiversitas 27 (3): d270320. https://doi.org/10.13057/biodiv/d270320. Extraintestinal pathogenic Escherichia coli (ExPEC) is a leading cause of bloodstream infections (BSIs) worldwide and contributes substantially to the burden of antimicrobial resistance. The aim of this study was to evaluate the concordance between phenotypic antimicrobial susceptibility testing (AST) and genotypic resistance determinants identified by whole-genome sequencing (WGS) in E. coli BSI isolates. During the study period, 194 blood culture samples from patients with suspected BSIs were processed, of which 92 yielded positive results; 13 non-duplicate E. coli isolates (14.13% of positive cultures) were included in this single-center, exploratory study. Phenotypic resistance profiles were determined using the VITEK®2 Compact system, while WGS was performed to identify antimicrobial resistance genes, sequence types (STs), and resistance-associated mutations. Phenotypic resistance showed that resistance to ampicillin (7/13, 53.8%), ciprofloxacin (6/13, 46.2%), and ceftriaxone (5/13, 38.5%). Genomic analysis revealed β-lactam resistance genes predominantly associated with blaTEM-1B and its variants (blaTEM-116, blaTEM-141, and blaTEM-206), along with blaCTX-M-55; blaTEM-1B was the most frequently detected gene (4/13), followed by blaCTX-M-55 (2/13). Fluoroquinolone resistance was mainly linked to mutations within quinolone resistance-determining regions of gyrA and parC, as well as alterations affecting the AcrAB-TolC efflux system. Multilocus sequence typing using the Achtman scheme demonstrated high genetic diversity, with 12 distinct STs identified among 13 isolates, including ST73, ST117, ST410, ST95, and ST127. High concordance between phenotypic β-lactam resistance and genotypic findings was observed, as all β-lactam-resistant isolates carried at least one corresponding β-lactamase gene. Overall, these findings indicate that WGS can reliably confirm β-lactam resistance mechanisms and provide useful molecular epidemiological insights into E. coli isolates causing BSIs; however, conclusions are limited by the small sample size and single-center design, and larger multicenter studies are warranted to support routine surveillance applications.
Detection Of Bordetella Pertussis the Cause of Whooping Cough: A Narrative Review Jongga Adiyaksa; Conny Riana Tjampakasari
Biomedika Vol 16, No 1 (2024): Biomedika Februari 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v16i1.2799

Abstract

Bordetella Perussis transmits pertussis disease, known as whooping cough, through airborne secretions from the respiratory tract. The bacteria then attach to the epithelial cilia of the respiratory tract of other hosts. One of the main virulence factors produced by Bordetella pertussis is pertussis toxin (PTx) which in its inactivated form is the main component of all acellular pertussis vaccines. In addition, PTx also activates several receptors and can affect various signaling pathways independent of ADP ribosylation and adenylate cyclase. PTx has been used in many research fields due to its strong ADP-ribosylation properties. To detect PTx Bordetella pertussis can use diagnostic tests such as serology test. Aim of this literary study is to determine the types of examinations for the detection of Bordetella pertussis. This study used a narrative review method sourced from searches on PubMed and ScienceDirect. The results of this search showed various types of Bordetella pertussis detection, including detection by culture, serology, and PCR. In conclusion, culture is still recommended to assess vaccine efficacy and future vaccine development, serological tests are one of the main diagnostic procedures in cases of unvaccinated adolescents and adults using specific B. pertussis proteins as antigens, in the presence of increased IgA or IgG titers against the toxin pertussis (PT) and filamentous hemmagglutinin (FHA). PCR as a diagnostic tool for pertussis in neonates and children.
Bakteri Gram positif Listeria monocytogenes sebagai Penyebab Food-borne Disease Riana Tjampakasari, Conny
Cermin Dunia Kedokteran Vol 48 No 1 (2021): Infeksi COVID-19
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v48i1.29

Abstract

Bakteri Listeria monocytogenes (L. monocytogenes) merupakan salah satu patogen food-borne penyebab listeriosis dengan prevalensi rendah, namun dengan fatality tinggi sebagai pencemar makanan. Listeriosis merupakan penyakit infeksius yang menyebabkan aborsi, meningitis, dan septikemia; cenderung lebih fatal pada kelompok populasi ibu hamil, bayi, lanjut usia, serta penderita imunodefisiensi. Meskipun kasus infeksi bakteri ini jarang, namun klinisnya termasuk parah dengan angka kematian tinggi. Deteksi laboratorium untuk diagnosis dapat dengan metode analytic konvensional dan metode cepat. Upaya pengendalian infeksi bakteri ini adalah dengan pencegahan dan pengobatan infeksi. Listeria monocytogenes (L. monocytogenes) bacteria is a food-borne pathogen with a low prevalence rate but with a high fatality rate as a food contaminant. Listeriosis is an infectious disease that causes abortion, meningitis and septicemis; tends to be more fatal in population of pregnant women, infants, the elderlies, and immunodeficiency sufferers. Although cases are rare, the clinical conditions are categorized as severe with a high mortality rate. Laboratory diagnosis can be done with conventional analytic and rapid methods. Infections can be controlled with prevention and treatment.
Bakteri Anaerob Clostridium botulinum dan Toksin yang Dihasilkannya Tjampakasari, Conny Riana; Hanifah, Rifdah
Cermin Dunia Kedokteran Vol 49 No 5 (2022): Neuro-Kardiovaskular
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v49i5.230

Abstract

C. botulinum adalah salah satu bakteri paling patogen karena dapat menghasilkan botulinum neurotoxin (BoNT) yang mematikan. Terdapat 3 jenis botulisme, yaitu botulisme keracunan makanan, botulisme inhalasi, dan botulisme luka. Meskipun kejadian botulisme jarang, namun harus diwaspadai karena cukup fatal. Sebagian besar kasus botulisme pada manusia disebabkan oleh makanan kaleng yang dipersiapkan di rumah. Isolasi dan identifikasi C. botulinum dapat dilakukan dengan pemeriksaan pewarnaan Gram, kultur, dan identifikasi, sedangkan deteksi toksin dapat menggunakan metode mouse lethality assay, non-lethal mouse assay, dan metode imunologi. Pendekatan molekuler dilakukan melalui uji polymerase chain reaction (PCR) untuk deteksi jenis toksin. Pencegahan botulisme dilakukan dengan teknik penanganan makanan yang tepat. Pemanasan yang memadai dapat membunuh spora bakteri, selain itu segera mengonsumsi makanan yang telah dimasak dapat mencegah C. botulinum bertumbuh. C. botulinum is one of the most pathogenic bacteria because it can produce deadly botulinum neutotoxin (BoNT). There are 3 types of botulism: botulism poisoning, inhalation botulism, and wound botulism. Although the incidence of botulism is rare, the impact is quite fatal. Most cases of botulism in human are caused by canned food prepared at home. Isolation and identification of C. botulinum can be done by Gram staining, culture, and identification, while the detection of toxins can use the mouse lethality assay, non-lethal mouse assay, and immunological methods. Molecular approach can be done through polymerase chain reaction (PCR) examination to detect the toxin type. Prevention can be applied with proper food handling techniques. Adequate heating can kill bacterial spores, and direct consumption can prevent proliferation of C. botulinum.
Virulence Factor of Leptospira sp. Causes of Leptospirosis and Its Management Conny Riana Tjampakasari; Wisiva Tofriska Paramaiswari
Formosa Journal of Science and Technology Vol. 4 No. 3 (2025): March 2025
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/fjst.v4i3.36

Abstract

Leptospirosis is often found in tropical and subtropical countries with high rainfall. Leptospirosis outbreaks have been reported throughout the world and in Indonesia is still a problem with the outbreak of several Extraordinary Events in various regions. This bacterium has several virulence factors, including Leptopsiral Microbial Surface Component Recognizing Adhesive Matrix Molecules (MSCRAMM), Lipopolisakarida (LPS), Loa22, LipL32 or hemolysis-associated protein I (Hap I), Protein Len/Protein Lsa24, Protein LigA (Leptospiral Immunoglobulin-Like Proteins) and HemO. The diagnosis of leptospirosis is made based on laboratory examination, but not all countries, especially developing countries, are able to carry out examinations for Leptospira sp., so many cases go unreported. By studying the role of virulence factors, it is hoped that the self-defense mechanisms of bacteria in the host can be identified so that they can increase the potential to prevent their pathogenicity from causing disease. Control of Leptospirosis consists of primary prevention, namely protection of healthy people from VOID Leptospirosis, promotive in nature, and specific protection by means of vaccination; secondary prevention targets people who are already with Leptospira, by preventing complications that can cause death. Efforts to control Leptospirosis must be implemented thoroughly so that outbreaks do not continue.
Co-Authors Abas, Ghina Mutiara Alfia Chairunnisa Andi Yasmon Anis Karuniawati Ariyani Kiranasari Ariyani Kiranasari Arleni Bustami Aulia Rosalina, Regita Baswara, R. Muh. Kevin Chrecentia Hanna Swestikaputri Cynthia Gozali Delly Chipta Lestari Engel, Yulia Fathia Ramadhani Fatindhiya Mahira Hilmy Febrianti, Tati Hanayurianingtyas, Deajeng Laras Hanifah, Nadyatul Hanna Swestikaputri, Chrecentia Herjuno Ari Nugroho Ichwan Baihaki Jongga Adiyaksa Kiranasari, Aryani LANY STEVINA NABILA NAURA Nina Herlina Nova Dilla Yanthi Paramita, Rafika Indah Pratama, Vanessa Maritza Putri, Mariza Nabila Raden Mohamad Herdian Bhakti RAFIKA INDAH PARAMITA Regita Aulia Rosalina Riani Agustini Rida Tiffarent Rida Tiffarent Rifdah Hanifah Rosa, Yulia Rosdiana Irawati Shoffiana Noor Siti Kusmaryeni SJATHA, FITHRIYAH Stevina, Lany Sudarmo, Fitrahwati Sugiyono . Syaiful Rizal Thalia Anggrea Noor Tjahjani Mirawati Sudiro Windi Muziasari Wisiva Tofriska Paramaiswari