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Abdul Hakim Fattah
Universitas Muhammadiyah Sinjai
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Social Sciences Veterinary

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Journal : Jurnal Agripet

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Addition of Sodium Hyaluronate to Ringer Lactate-Egg Yolk Diluent on the Quality of Native Chicken Sperm Khaeruddin, Khaeruddin; Fattah, Abdul Hakim; Syamsuryadi, Bahri; Hermawansyah, Hermawansyah
Jurnal Agripet Vol 25, No 1 (2025): Volume 25, No. 1, April 2025
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v25i1.41814

Abstract

This study aims to evaluate the effect of adding varying concentrations of sodium hyaluronate (SH) to a Ringer lactate-egg yolk diluent on the quality of native chicken spermatozoa during 48 hours of storage. A completely randomized design (CRD) was used with four treatment groups: control (diluent without SH), diluent with 63 M SH, 103 M SH, and 143 M SH. The study involved six replications with pooled semen from three native chickens aged approximately 10 months. Semen was collected, diluted, and stored at 5C for 48 hours. Parameters observed after storage included motility, viability, plasma membrane integrity, acrosome integrity, DNA damage, and mitochondrial activity. Data were analyzed using analysis of variance (ANOVA). The results indicated that the treatments had no significant effect (P0.05) on any of the spermatozoa quality parameters. The observed values for motility, viability, plasma membrane integrity, acrosome integrity, DNA damage, and mitochondrial activity were 46.67-50%, 95.11-96.55%, 92.77-95.55%, 4.77-5.44%, and 71.05-75.37%, respectively. In conclusion, the addition of sodium hyaluronate at concentrations of 63-143 M in a Ringer lactate-egg yolk diluent does not significantly affect the quality of native chicken spermatozoa during 48 hours of storage at 5C.
Co-Authors Amin, Muhtar Andi Harnita Nurul Amalia Andi Kurnia Armayanti Andi Nurlinda Arifin Arifin Armayanti, Andi Kurnia Azmi Mangalisu Bahri Syamsuryadi Budianto, Rachmat Dian Yustisia Faridah, Rajmi Hasfina, Hasfina Hermawansyah, Hermawansyah Irsang Irsang Junaedi Junaedi Khaeruddin Khaeruddin Khaeruddin Khaeruddin M. Musdalifa Mayasari, Andini Indah Muhammad Erik Kurniawan Muhammad Nur Hidayat N. Nurdiana Nurdin, Fadilah Nurfiana, Rika Pardi Pardi Putra Astaman Rajmi Faridah Rika Nurfiana Sasa, Lusi Yunita Siska Milanda Syahrir Syahrir syamsidar syamsidar Syamsuryadi, Bahri