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Exploration of Nematophagous Fungi from Coffee Rhizosphere Soil and their Potential as Biological Control Agents against Root-lesion and Root-knot Nematodes Indarti, Siwi; Aldina, Rinda Fajrin; Widianto, Donny; Prijambada, Irfan Dwidya; Maharani, Rina; Kurniasari, Irianti; Waele, Dirk De
Jurnal Perlindungan Tanaman Indonesia Vol 29, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.102469

Abstract

Root-lesion and root-knot nematodes are amongst the most important pathogens of coffee and can cause substantial yield losses and quality reductions. Environmental and health concerns concerning the use of chemical pesticides have increased the need for alternative management strategies against plant-parasitic nematodes. The aim of our study was to isolate and identify nematophagous fungi from nematode-infested coffee production areas and evaluate their potential as biocontrol agents. Our study was carried out in two stages: 1) fungi isolation and evaluation of their ability to affect eggs or vermiform developmental stages of root-lesion and root-knot nematodes; 2) identification of fungal isolates to species level. Eleven fungal isolates were able to affect either the nematode eggs or the vermiform developmental stages. The ability of these fungi to produce extracellular enzymes were also evaluated. This study highlights Indonesian nematode infected coffee rhizosphere soils as a rich source of nematophagous fungi, with eleven isolates showing promises to be used for integrated pest management strategies. Future work should assess field efficacy under local conditions while monitoring impacts on soil food webs and non-target organisms.
Toxicity Tests of Botanical Pesticide Made of Tuba Root Extract on Spodoptera frugiperda Ibrahim, Mukhlis; Martono, Edhi; Indarti, Siwi
Agrotechnology Research Journal Vol 7, No 2 (2023): Agrotechnology Research Jurnal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrotechresj.v7i2.77815

Abstract

One of the main pests that attack corn plants is Spodoptera frugiperda J. E. Smith. The usual control of this pest is by using synthetic insecticides, but the continuous or unwise use of synthetic insecticides will have negative impacts on humans and the environment, therefore alternative insecticides that are safe and environmentally friendly, such as tuba root (Derris elliptica Benth) are needed. This study aims to determine the effect of tuba root extract phytopesticide on S. frugiperda larvae and to determine the concentration of tuba root extract that is effective in controlling S. frugiperda larvae, as well as the eating inhibition power of S. frugiperda larvae after administration. The research was conducted at the Applied Entomology Laboratory, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta from September to November 2022. The treatment concentrations used were 0, 2, 4, and 8 mL.L-1 of water. The experiment employed Completely Randomized Design (CRD) with four treatments and five replications. The results showed that the concentration of 4 mL.L-1 of water is an effective concentration for controlling S. frugiperda larvae and can cause a total mortality of 82% with an initial death of 19 hours, Lethal Time 50 at 39.20 hours after application. In addition, the concentration of tuba root powder extract of 8 mL.L-1 of water appeared to be the highest in reducing the appetite of larvae compared to other treatments with a rate of 17.03%. This showed the highest reduction in larval appetite compared to other treatments. A small amount of feed eaten means a high level of antifeedant activity.