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All Journal HAYATI Journal of Biosciences Biogenesis: Jurnal Ilmiah Biologi Biology, Medicine, & Natural Product Chemistry Majalah Obat Tradisional Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Journal of Tropical Biodiversity and Biotechnology Jurnal Biodjati Makara Journal of Science Journal of Biotechnology and Natural Science Molekul: Jurnal Ilmiah Kimia Berkala Ilmiah Biologi

Dwi Umi Siswanti

Faculty Of Biology, Universitas Gadjah Mada

Author-ID : 484172

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Physiological Response of 'Segreng' Rice Plant (Oryza sativa L.) to Biogas Sludge at Wukirsari Village, Cangkringan, Sleman Dwi Umi Siswanti; Nindy Senissia Asri; Mifta Arlinda; Arianda Poetri Shofia Rochman; Akrima Syahidah
Biology, Medicine, & Natural Product Chemistry Vol 7, No 1 (2018)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Wukirsari Village, Cangkringan District is belong to Merapi Mountain’s slopes which located between the Gendol River and Yellow River. Nowadays, we faced the problem of anorganic fertilizer overused such as Urea, ZA, TSP/SP-36 and KCl in agriculture land. The effort to return the soil organic compound can be done by added some organic compounds or microbial bio -organic fertilizer. Sludge is fermented biodigester yield and it has lost its gas. The aim of this research was to understand the physiological response and optimum dose of biogas as planting medium to ‘Segreng’ Rice planted in the rice field of Wukirsari Village, Cangkringan District, Sleman Regency. This research was done on greenhouse scale and rice field scale. The treatment given on 0; 1; 1,5; 2 and 2,5 liters per 100 m2 of rice field areas, and given on 0; 4; 8; 12; and 24 ml per 5 kg soil on polybags. Data were taken in three repetitions. The vegetative growth parameters included plant height, number of leaves, number of seedlings and chlorophyll content, while generative growth parameters measured included NRA levels, dried biomass including crown/stem, roots, filled grains, empty grains, and total weight and number of filled grains, empty rains, and the number of panicles. The result were tested with ONE WAY ANOVA (Analysis of Variance) with SPSS version 19 for Windows and followed by Duncan's Multiple Range Test with 95% significance level (α = 0.05). Generally, the result showed that biogas sludge can increase the vegetative and generative growth of rice plant ‘Segreng’ on polybag scale and rice field scale. The rice plant on polybag with 4 ml biogas sludge was significantly different on the vegetative growth and chlorophyll content, while the rice plant on polybag with 8 ml biogas sludge was significantly different on the generative growth and NRA levels.

Pertanian Organik Terpadu di Desa Wukirsari, Sleman, Yogyakarta Sebagai Usaha Pemulihan Kesuburan Lahan Terimbas Erupsi Merapi 2010 dan Pencapaian Desa Mandiri Sejahtera Dwi Umi Siswanti
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 1 (2015): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Community service activities at Wukirsari village was preceded by many concerns land damaged by the eruption of Merapi 2010. Researchers encourage Wukirsari people to self-sufficient in the provision of liquid organic fertilizer and manure plus. It is intended to restore the soil nutrient that improves soil structure. The target of this activity were increasing the agricultural productivity, cost efficiency of production, improvement of agricultural cultivation, encouraging self-sufficiency of liquid organic fertilizer and manure plus and the establishment of agricultural enterprises. The conclusion of these program are the liquid organic fertilizer producted by Wukirsari farmer response positively to the growth and productivity of rice plants. The barriers of this program was delayed of the establishment of business entities will be overcome with the implementation of the third period (2015) of the community service program which cooperate with the Student Community Service Program (KKN PPM UGM).

Application of Bio Fertilizer and Biogas Sludge to Curly Red Chili (Capsicum annuum L.) at Wukirsari Village, Cangkringan, Sleman Dwi Umi Siswanti; Utaminingsih Utaminingsih; Melinda Fajar Lestari; Hamdian Hamdian
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 5, No 3 (2019): Desember
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Curly red chili (Capsicum sp.) is an important agricultural commodity in Indonesia. The pattern of conventional cultivation using inorganic fertilizers has caused high production costs. While the level of spiciness of chili determined by capsaicin levels is strongly influenced by nutrients in the growing media. Bio fertilizer and sludge biogas biogas which is one of the organic fertilizers proved to be able to increase the growth and productivity of rice plants. This study aimed to analyze growth parameters, chlorophyll content and measure capsaicin levels in curly red chili. Which given bio fertilizer and biogas sludge biogas in various dosage and determine the optimum dose of the fertilizer. The study was conducted at an agricultural demonstration plot in Wukirsari Village, Cangkringan, Sleman. The study design used RCBD (Randomized Complete Block Design), the data were analyzed by ANOVA (Analysis of Variance followed by Duncan's multiple distance test (DMRT) at the 95% confidence level. The highest growth parameters was obtained on curly red chili plants which were given sludge biogas 36 mL + bio fertilizer 10 L / ha. Thus, it can be concluded that the most appropriate dose of curly red chili is 36 mL sludge biogas + bio fertilizer 10 L/ha.Keywords: Bio-fertilizer; biogas; chili; growth;sludge; Wukirsari.

Effect of Alkaline and Drought Stress on Growth and SOD (Superoxide dismutase) Content in Basil Plant (Ocimum americanum L.) Anindita Della Rosa Riyadi; Dwi Umi Siswanti
Jurnal Biodjati Vol 7, No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.16680

Basil plant (Ocimum americanum L.) is a fragrant annual plant widely cultivated by the people in Indonesia because it is useful and commercial. Previous research on salinity and drought stress in basil affects growth and development plant, cell turgor pressure, and the anatomical structure of the plant. However, research on the effect of alkalinity and drought stress in basil plants has not been carried out. Therefore, this study was conducted to determine the effect of variations in alkaline and drought stress on the growth and superoxide dismutase (SOD) content of basil plants. Alkalinity stress was carried out by variations of dolomite (D) doses which is divided into four levels, namely D1, D2, D3, and D4 (0 grams/pot, 100 grams/pot, 150 grams/pot, and 200 grams/pot) and variations of drought stress were divided into four types of field capacity, namely A1, A2, A3, and A4 (25%, 50%, 75%, and 100%). Parameters measured were plant height, leaf length and width, number of leaves, fresh and dry weight, SOD content, and environmental parameters. The growth of the basil plant (O. americanum L.) decreased after being given drought stress at a field capacity level of 25%, while the SOD content of basil (O. americanum L.) did not differ significantly but increased with the increase in dolomite doses and field capacity given.

Growth response, chlorophyll content, and nitrate reductase activity of mustard greens (Brassica rapa L.) to salinity stress post application of biofertilizer in hydroponic system Hasna Fatin Affifah; Dwi Umi Siswanti
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 2 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i2.29505

Effect of Mercury Stress on the Growth and Lipid Content of Euglena sp. and Echinodorus palaefolius Wisnu Eka Wardana; Dea Hastaning Tantri; Marshanda Nur Roosyana Afifah; Hasna Nur Aini; Dwi Umi Siswanti; Khusnul Qonita Maghfiroh; Tia Erfianti; Ria Amelia; Dedy Kurnianto; Eko Agus Suyono
Jurnal Biodjati Vol 8, No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.23764

One way to reduce the adverse effects of the heavy metals mercury in the aquatic environment are using organisms to break down or convert toxic substances into non-toxic forms, either by phytore- mediation or phycoremediation. This research aimed to analyze the growth and lipid content of Euglena sp. after mercury exposure. This research also aimed to analyze the growth of E. palaefolius which is associated with Euglena sp. In this study, the bioremediation ability of Euglena sp. and Echinodorus palaefolius through treatment with mercury concentrations of 5 ppm, 10 ppm, 15 ppm, and 20 ppm, as well as association and non-association treatments. The parameters are the growth of Euglena sp. and the association between Euglena sp. andE. palaefolius measurement and lipid content. The result of the growth of Euglena sp. experienced a significant increase. Lipid content in Euglena sp. was also seen high at 10 ppm mercury concentration. In E. palaefolius, the ability to adsorb heavy metals was also shown by the large diameter of the stems and also the plant growth which has optimal growth in the treatment of 10 ppm mercury stress.

The Effect of Acidic pH on Growth Kinetics, Biomass Productivity, and Prima-ry Metabolite Contents of Euglena sp. Nurafifah, Istini; Hardianto, Muhammad Andhi; Erfianti, Tia; Amelia, Ria; Maghfiroh, Khusnul Qonita; Kurnianto, Dedy; Siswanti, Dwi Umi; Sadewo, Brilian Ryan; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
Makara Journal of Science Vol. 27, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Euglena is a microalga with the potential to be an environmentally friendly renewable energy resource. The pH value is a crucial factor in micro-algal cultivation. Changes in pH affect the growth and development of microalgae, including the production of biomass and primary metabolites, such as proteins, carbohydrates, and lipids. In this study, Euglena sp. was grown on Cramer-Myers medium and subjected to various acidic conditions. This study aimed to determine the effect of pH on the growth kinetics, biomass, carbohydrate, lipid, and protein contents of Euglena sp. The Euglena sp. culture was optimized at various pH values of 2.5, 3.5, and 4.5. The results were analyzed by one-way analysis of variance at a 95% confidence level, followed by Duncan’s multiple range test. As results, Euglena sp. had the best growth rate, the greatest biomass, and the highest carbohydrate, protein, and lipid contents at pH 3.5 compared to the other pH conditions. The average biomass in the pH 3.5 treatment was 1.600 ± 0.229 g/L, and the carbohydrate, protein, and lipid contents were 5.983 ± 0.056 g/L, 0.196 ± 0.023 µg/mL, and 0.300 ± 0.020 g/L, respectively.

The Development of Antioxidant Nutraceuticals containing Chrysanthemum indicum L. Gummy Candy Rahmasari, Firdaus Salvia; Sahid, Muhammad Novrizal Abdi; Siswanti, Dwi Umi; Darsih, Cici; Utami, Indrawati Dian; Alam, Lucky Prabowo Miftachul; Laksitorini, Marlyn Dian
Majalah Obat Tradisional Vol 29, No 1 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.87112

Chrysanthemum flowers (Chrysanthemum indicum L.) have been shown to contain flavonoid compounds and exert antioxidant activity. This brings Chrysanthemum indicum potential to be developed as a nutraceutical product. This study aims to evaluate the antioxidant activity of Chrysanthemum flowers before and after the formulation of Chrysanthemum indicum flower extract as a gummy candy. Gummy candy formulas were developed using variations of gelatin and pectin as gelling agents. In this study, Chrysanthemum flower extract was formulated into gummy candy. The physical characteristics evaluated include organoleptic tests, weight uniformity, elasticity, and moisture content. Optimization was performed using the simplex lattice design (SLD) method with the aid of the Design Expert software Ver. 13. The antioxidant activity of the chrysanthemum flower extract and gummy candy extract was evaluated using the DPPH radical scavenging method. Ascorbic acid was used as a positive control. The optimum formula for preparing the gummy candy was 11.51% of gelatin and 1.24% of pectin. The evaluation of weight uniformity, elasticity, and moisture content suggested that there is no significant difference between the optimum formula and the predicted value. Both the chrysanthemum flower extract and chrysanthemum flower gummy candy had strong antioxidant activity. The IC50 value of the extract was 67.80 ± 2.37 mg/mL while the gummy candy IC50 value was 82.93 ± 2.55 mg/mL. The antioxidant activity of Chrysanthemum indicum was slightly decreased after being formulated into gummy candy. These studies suggested that scientists are expected to anticipate the decrease of Chrysanthemum antioxidant activity in the gummy manufacturing process.

Response of root anatomy and vitamin C content of Brassica juncea L. on biofertilizer application in a saline environment Palupi, Dwi Rekno; Siswanti, Dwi Umi
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 2 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i2.36522

Indian mustard (Brassica juncea L.) is part of the family Brassicaceae which has an annual-herbaceous habitus and is counted as a popular vegetable commodity in Indonesia so it has a high-demand market. To fulfill the demand market can be done by hydroponic cultivation and biofertilizer application. Application of biofertilizer which contains inoculant microorganisms can support plant growth with increased availability of nutrients in planting medium and neutralized salinity stress effect. Salinity stress is indicated by the high concentration of Na+ and Cl- ions in plants resulting in toxicity and osmotic stress which will inhibit plant growth. This study aimed to determine the effect of various dosages of biofertilizer on plant growth and productivity with plant height, leaf number, cortex thickness, metaxylem diameter, and ascorbic acid levels as parameters. This research used cow urine base biofertilizer containing nitrogen (N), phosphate (P), kalium (K), and calcium (Ca). There are a few treatments: negative control, positive control, biofertilizer treatment 40 mL, 80 mL, and 120 mL dosage, and salinity treatment by applying NaCl 5000 ppm 10 mL/system. As the result showed on 120 mL biofertilizer dosage has the highest mean of plant height and leaves number, and 80 ml biofertilizer dosage treatment has the highest average of cortex thickness and metaxylem diameter, but the various dosage of biofertilizer treatment did not have a significant effect on ascorbic acid levels increase. Growth in plants given biofertilizer is higher than the plant growth that grew in the negative control treatment. It can be concluded that the application of biofertilizer can support the growth of B. juncea L. in the hydroponic system under salinity stress.

Bioremediation of Mercury- Polluted Water in Free Water Surface-Constructed Wetland System by Euglena sp. and Echinodorus palifolius (Nees & Mart.) J.F. Macbr. Siswanti, Dwi Umi; Daryono, Budi Setiadi; Petrus, Himawan Tri Bayu Murti; Suyono, Eko Agus
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.88143

Mercury accumulation in the aquatic environment can be highly harmful. The body takes mercury vapor through the lungs, then absorbs mercury metal through the digestive system, and then the blood carries the metal to the brain. Bioremediation is the process of breaking down or converting harmful compounds into non-toxic forms, which can be accomplished through phytoremediation or phycoremediation. The goal of this study was to examine the growth and anatomy of Euglena sp. after being cultured in the mercury-containing FWS-CW waste treatment system. The ability of Euglena sp. and Echinodorus palifolius to bioremediate mercury at different concentration as well as association and non-association treatments. This study was carried out in a bioreactor known as FSW-CW (Free Water Surface-Constructed Wetlands). Plant growth (plant height and number of leaves), chlorophyll content, diameter of root and petiole, metaxylem diameter of root, petiole, and leaves, cortical thickness of root and leaves, and petiole anatomy were all measured. Water temperature, pH, salinity, and light intensity were all measured as environmental parameters. Mercury treatment reduced Euglena density (183.5 cells. mL-1103 in control and 12.6 cells. mL-1103 in 100 ppm mercury treatment) and number of E. palifolius leaves, but not plant height and chlorophyll. Root and petiole diameters were affected by the mercury treatment, petiole diameter decreased unless the concentration was 100 ppm, whereas root diameter actually increased. The diameter of the root metaxylem increased, but the petioles and leaves, as well as the thickness of the root cortex, did not provide a significant response. The growth of E. palifolius was still optimal in the presence of Euglena in mercury-containing medium.

Co-Authors Afifah, Marshanda Nur Roosyana Aini, Hasna Nur Akrima Syahidah Akrima Syahidah Alam, Lucky Prabowo Miftachul Amelia, Ria Ananmaimuna, Jovika Syifa Anindita Della Rosa Riyadi Aprida Niken Arianda Poetri Shofia Rochman Azkiya, Nala Azzizah, Rikka Nur BUDI SETIADI DARYONO Cici Darsih Dea Hastaning Tantri Dedy Kurnianto Diah Rachmawati Eko Agus Suyono Eko Agus Suyono Erfianti, Tia Fitria Yulia Rachmawati Hamdian Hamdian Hardianto, Muhammad Andhi Hari Purwanto Hasna Fatin Affifah Hasna Nur Aini Himawan Tri Bayu Murti Petrus Khusnul Qonita Maghfiroh Kurnianto, Dedy Lena Mardiana Lestari, Melinda Fajar Lestari, Nurrisma Ika Maghfiroh, Khusnul Qonita Malika, Pradhika Cikal Marlyn Dian Laksitorini, Marlyn Dian Marshanda Nur Roosyana Afifah Maryani Maryani Melinda Fajar Lestari Melinda Fajar Lestari Mifta Arlinda Nadhifa Athaya Khairunnisa Niken Wulansari Nilamsari, Regina Nindy Senissia Asri Nur Hidayah Pangestuti Nurafifah, Istini Nurhanifah, Tsurayya Okky Shavira Riesty Palupi, Dwi Rekno Putri, Renata Adaranyssa Egistha Rahmasari, Firdaus Salvia Rain, Oryza Enwiera Rega Virgiyana Agustin Rega Virgiyana Agustin Rega Virgiyana Agustin Ria Amelia Riyadi, Anindita Della Rosa Sadewo, Brilian Ryan Sahid, Muhammad Novrizal Abdi Sinambela, Benn Gerson Juan Moreno Soenarwan Hery Poerwanto, Soenarwan Hery Solly Aryza Sudjino Sudjino Tantri, Dea Hastaning Tia Erfianti Untari, Ludmilla Fitri Utami, Indrawati Dian Utaminingsih Utaminingsih Vidhantyka, K.H. Mahadhevy Ryrathna Wardana, Wisnu Eka Wisnu Eka Wardana

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