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All Journal HAYATI Journal of Biosciences Biogenesis: Jurnal Ilmiah Biologi Biology, Medicine, & Natural Product Chemistry Majalah Obat Tradisional Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Journal of Tropical Biodiversity and Biotechnology Jurnal Biodjati Makara Journal of Science Journal of Biotechnology and Natural Science Molekul: Jurnal Ilmiah Kimia Berkala Ilmiah Biologi
Dwi Umi Siswanti
Faculty Of Biology, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Physiological and Anatomical Responses of Red onion (Allium cepa L.) to Drought Stress after Biofertilizer Application Lestari, Nurrisma Ika; Siswanti, Dwi Umi
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.38613

Abstract

Red onion (Allium cepa) is a plant that requires sufficient water and is susceptible to drought stress. The use of biofertilizer is employed because it contains microorganisms that enhance nutrient availability and assist plant growth under abiotic stress conditions. This study aims to analyze the physiological and anatomical responses of red onion bulbs following the application of biofertilizer under drought stress. The doses of biofertilizer used were 0; 10;15; and 20 L/ha with field capacity levels of 25; 50; 75 and 100%. The parameters tested were bulb diameter, reducing sugar content number of bulb layers, anatomy of root cortex thickness, anatomy of root metaxylem diam Red onion eter, and fresh weight of red onion roots. The method used in determining the level of reducing sugar is the Nelson-Somogyi method. The method used for preparing the red onion root specimens was the embedding method. The results showed that the largest red onion bulb diameter was obtained from treatment A1B2, without the addition of biofertilizer at 75% field capacity. Meanwhile, the highest reduced sugar content was found at a dose of 15 L/ha with 50% field capacity
Ethnopharmacological Study of Medicinal Plants in Kedungpoh Village, Nglipar Sub-district, Gunungkidul District, Special Region of Yogyakarta Untari, Ludmilla Fitri; Vidhantyka, K.H. Mahadhevy Ryrathna; Ananmaimuna, Jovika Syifa; Nilamsari, Regina; Malika, Pradhika Cikal; Poerwanto, Soenarwan Hery; Siswanti, Dwi Umi; Purwanto, Hari
Journal of Biotechnology and Natural Science Vol. 5 No. 1 (2025): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v5i1.12972

Abstract

The scientific study of ethnopharmacology investigates the medicinal uses of plants by different ethnic groups and plays a crucial role in the early-stage drug discovery process by identifying potential drug candidates from traditional remedies. The transmission of knowledge concerning the diversity of plant species and their utilization is typically an intergenerational process, facilitating the preservation of cultural heritage and the transfer of experiential knowledge. The present study was conducted in the village of Kedungpoh, Nglipar, Gunungkidul. The objective of this study is to investigate the utilisation, management and comprehension of medicinal plants within the Kedungpoh village in Gunungkidul.The research methodology comprised of observational studies and interviews with local residents. The results revealed the utilization of 24 plant species, which classified into 17 distinct families by the local community. The utilization of these plants encompassed various plant organs, including leaves, stems, roots, fruits, and rhizomes, which were employed in different processing methods, such as crushing, boiling, or rubed. The most prevalent and utilized plant families were Asteraceae, which are notable for their abundance of bioactive compounds, including flavonoids, alkaloids, and essential oils, which have been demonstrated to be efficacious in addressing a wide range of health concerns. This study provides substantial support for scientific laboratory research into the safety, quality and efficacy of traditional medicine practices, with a view to further utilization of traditional medicine, whilst also protecting the intellectual property rights of traditional knowledge holders.
Effect of Mercury Dose Variation on Growth and Nitrate Reductase Activity in Aquarius palifolius (Nees & Mart.) Christenh. & Byng Nurhanifah, Tsurayya; Siswanti, Dwi Umi
Makara Journal of Science Vol. 29, No. 2
Publisher : UI Scholars Hub

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Abstract

Mercury, a byproduct of mining, can harm the environment. One method to reduce this impact is through phytoremediation. This study used the Mexican sword (Aquarius palifolius), a plant with the potential to absorb and accumulate mercury. The aim of this study was to examine the effect of mercury stress on nitrate reductase activity, which plays a role in plant productivity, and to assess its impact on growth parameters, including plant height growth rate, leaf length, and number of leaves. Three-month-old A. palifolius plants obtained from rice fields in Sleman were treated with mercury at doses of 0, 14, 16, 18, and 20 ppm. The experiment was conducted in triplicate. Nitrate reductase activity was measured using a spectrophotometer, and growth rate was observed over 15 days. Data were analyzed using One-way analysis of variance (ANOVA) and evaluated using Duncan’s Multiple Range Test (DMRT). Mercury exposure led to adaptations, including a 9.5%–13.5% change in nitrate reductase activity and a 50%–65% reduction in the number of leaves. Growth rates of plant height and number of leaves showed no significant differences, even under mercury stress. These results indicate that A. palifolius can tolerate mercury concentrations of 14–20 ppm and has the potential to functions as a phytoremediation agent.
Bioremediasi Fosfat Limbah Detergen dan Model Kinetika Reaksi SPIROFILTER Berbasis Spirogyra porticalis (O.F. Mueller) Cleve Siswanti, Dwi Umi; Rain, Oryza Enwiera; Sinambela, Benn Gerson Juan Moreno
Berkala Ilmiah Biologi Vol 16 No 1 (2025)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v16i1.20524

Abstract

Wastewater has become a source of environmental problems caused by the content of chemicals that are harmful to the environment, including detergent waste. Detergent is a cleaning agent that contains surfactants, phosphate compounds, and other additives. Efforts to handle this detergent waste have been carried out communally or on a large scale in a reactor called IPAL (Wastewater Treatment Plant). However, no effective household-scale IPAL prototype has been found to restore wastewater quality to raw water that can be reused. So a prototype IPAL was built called SPIROFILTER which combines the filtration process by zeolite, activated charcoal, and cotton as well as hyperaccumulation by Spirogyra porticalis. This study aims to determine the role of S. porticalis, analyze wastewater quality, analyze the growth of S. porticalis, and determine the reaction kinetics model (PFO or PSO) in the absorption of phosphate in wastewater that has been filtered with SPIROFILTER. The research design was in the form of measuring the growth of S. porticalis and the quality of water before filtration (control), water after the 3rd day, and water after the 5th day. The parameters measured were temperature, pH, salinity, total phosphorus, total ammonia, BOD, COD, number of water germs, and chlorophyll content. This study used a UV-vis spectrophotometer and AAS for water quality testing. SPIROFILTER was an aquarium measuring 40x60x20 cm3 to which 132 mL of detergent was added in 22 liters of water. The results of the study proved that S. porticalis acted as a hyperaccumulator, the quality of water filtered by SPIROFILTER met water quality standards, the growth of S. porticalis remained optimum in detergent waste environmental conditions, the kinetic model of the phosphorus binding reaction from detergent waste used the Pseudo Second Order model in SPIROFILTER. Keywords: Detergent waste, Reaction kinetics, SPIROFILTER, Spirogyra porticalis, Water quality standards
Biofertilizers improve growth rate, nitrate reductase activity, and productivity of shallot (Allium cepa L.) under drought stress Lena Mardiana; Siswanti, Dwi Umi
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.57317

Abstract

Shallot (Allium cepa L.) is one of Indonesia's horticultural crops and represents as superior commodity. However, shallots are highly susceptible to drought and require sufficient water for growth. Biofertilizers contain various microbes that provide nutrients and increase the resistance of shallots to drought stress. This study aimed to determine the effect of biofertilizers on the growth rate, nitrate reductase activity, and productivity of shallot plants under drought stress. The research was conducted using a Factorial Completely Randomized Design with two factors. The first factor was the doses of biofertilizer with 0, 10, 15, and 20 L ha-1. The second factor was the drought stress with treatments of 25, 50, 75, and 100% field capacity. Parameters measured included plant height rate, leaf number rate, number of tillers, tuber wet weight, tuber dry weight, and nitrate reductase activity. Data were analyzed by ANOVA using SPSS followed Duncan’s Multiple Range Test (DMRT) at 5% significance level. The results showed that biofertilizer application did no significantly different on the growth rate, but significantly affected the number of tillers, tuber weight, and nitrate reductase activity of shallots under drought stress. The optimum dose of 10 L ha-1 biofertilizer increased the number of tillers (9.33). The optimum dose of 20 L ha-1 biofertilizer increased the growth rate, tuber wet weight (4.46 g), tuber dry weight (0.63 g), and nitrate reductase activity (1.11 µmol NO2- g-1 leaf wet weight h-1 of incubation). It is concluded that biofertilizer application, particularly at 10–20 L ha-1, can improve shallot performance under drought stress and is recommended as a drought mitigation strategy in shallot cultivation.
Biofertilizer application enhance chlorophyll content, membrane stability index, and anatomy of shallot (Allium cepa L.) leaves under drought stress Azkiya, Nala; Siswanti, Dwi Umi
Biogenesis: Jurnal Ilmiah Biologi Vol 14 No 1 (2026)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v14i1.58538

Abstract

Shallots (Allium cepa L.) are plants from the Amaryllidaceae family, Allioieae subfamily, and Allium genus. This perennial plant is estimated to have more than 1,000 species. Shallot leaves form a basal sheath. Biofertilizer contains rhizobacteria, which facilitate nutrient availability and uptake by plants. This research was conducted to determine the effect of biofertilizers on the physiological conditions and anatomical structure of shallot leaves (A. cepa L.) under drought-stress conditions. The treatments applied included the provision of biofertilizer and different drought stresses; biofertilizer was used with concentrations (10, 15, and 20 L/Ha), and the drought stress applied was 25, 50, and 75%. Data from measurements of chlorophyll a and chlorophyll b levels, Membrane Stability Index (MSI), stomatal density, leaf thickness, and metaxylem diameter were analyzed using SPSS 20, ANOVA test, DMRT with a confidence level of 95%. A biofertilizer concentration of 10 L/Ha is optimal for the Membrane Stability Index (MSI), a concentration of 15 L/Ha is optimal for chlorophyll a and b levels, leaf thickness, and metaxylem diameter, and a concentration of 20 L/Ha is optimal for increasing stomata density shallot leeks under drought stress conditions.
In Vitro Antioxidant and α-Glucosidase Inhibitor Metabolites of Chrysanthemum indicum Flower Represented by Molecular Networking Darsih, Cici; Azzizah, Rikka Nur; Siswanti, Dwi Umi; Laksitorini, Marlyn Dian
Molekul Vol 20 No 3 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.3.12831

Abstract

ABSTRACT. Chrysanthemum indicum flower is known as a Chinese medicinal plant and is consumed as a tea or food supplement. Several research reported the profiling metabolites of this flower using High-Performance Liquid Chromatography (HPLC) or Liquid Chromatography Mass Spectroscopy (LC-MS). However, there is a limitation of those methods, which are the yields several formula obtained by searching in database based on parent masses so give less effectiveness identify of compound. This study aimed to identify the metabolites in the ethanolic extract of C. indicum using a de-replication strategy by coupling Liquid Chromatography Orbitrap HRMS with a molecular network approach. This study also evaluated the total phenolic contents (TPC) using Folin-Ciocalteu method, Fourier Transfer Infrared (FTIR) Spectroscopy, while the scavenging activity against DPPH radical method was used to determine the antioxidant activity and the inhibition of α-glucosidase was conducted using α-glucosidase assay. The studies showed that there were diverse families of metabolites were putatively identified in C. indicum such as flavonoids and derivates, amino acids, fatty acids and derivates, phenol and derivates, terpenoids, and glucose. The detailed metabolites in extract were approached using application of molecular networking. In agreement with the molecular networking, the extract exerted strong antioxidant activity with % inhibition value of 76.20±1.35 at 100 µg/mL. Whereas, the α-glucosidase inhibitory activity showed good activity with % inhibition value of 83.04±0.52 at 50 µg/mL. The results of this study provide a new metabolite library for C. indicum ethanol extract as well as the confirmation of some of its biological activities. Keywords: Chrysanthemum, profiling metabolites, LC-HRMS
Growth Rate and Capsaicin Level of Curly Red Chili (Capsicum annum L.) on Biofertilizer and Biogas Sludge Application Dwi Umi Siswanti; Melinda Fajar Lestari
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4216

Abstract

Curly red chili (Capsicum annum L.) is widely cultivated us-ing inorganic fertilizers which causes high production costs. Chili is valued because of the level of spiciness. The level of spiciness of chili is determined by the level of capsaicin which is affected by nutrients in the growing media. The nutrient of the planting media can be fulfilled by degrading the growing media by microbes in organic fertilizer. One type of organic fertilizer containing degrading microbes is biofertiliz-er. Biofertilizer and biogas sludge is one of the organic fertilizers con-taining microbes and organic materials that are able to support nutri-ent levels of the planting medium. This study aimed to analyze growth parameters include chlorophyll content and measure capsaicin levels in curly red chili. Which given biofertilizer and biogas sludge in vari-ous dosage and determine the optimum dose of the fertilizer. The study was conducted at an agricultural demonstration plot in Wukirsari Vil-lage, Cangkringan, Sleman. The study design used RCBD (Randomized Complete Block Design), the data were analyzed by ANOVA (Analysis of Variance followed by Duncan’s Multiple Distance Test (DMRT) at the 95% confidence level. The highest growth parameters and capsa-icin content were obtained on curly red chili plants which were given biogas sludge 36 mL + biofertilizer 10 L / ha. The application of bi-ofertilizer and biogas sludge in various concentrations given has not been able to increase the average total chlorophyll content of curly red chili plants. Thus, it can be concluded that the most appropriate dose of curly red chili is 36 mL biogas sludge + 10 L bio fertilizer/ha
Effect of Alkaline and Drought Stress on Growth and SOD (Superoxide dismutase) Content in Basil Plant (Ocimum americanum L.) Anindita Della Rosa Riyadi; Dwi Umi Siswanti
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.16680

Abstract

Basil plant (Ocimum americanum L.) is a fragrant annual plant widely cultivated by the people in Indonesia because it is useful and commercial. Previous research on salinity and drought stress in basil affects growth and development plant, cell turgor pressure, and the anatomical structure of the plant. However, research on the effect of alkalinity and drought stress in basil plants has not been carried out. Therefore, this study was conducted to determine the effect of variations in alkaline and drought stress on the growth and superoxide dismutase (SOD) content of basil plants. Alkalinity stress was carried out by variations of dolomite (D) doses which is divided into four levels, namely D1, D2, D3, and D4 (0 grams/pot, 100 grams/pot, 150 grams/pot, and 200 grams/pot) and variations of drought stress were divided into four types of field capacity, namely A1, A2, A3, and A4 (25%, 50%, 75%, and 100%). Parameters measured were plant height, leaf length and width, number of leaves, fresh and dry weight, SOD content, and environmental parameters. The growth of the basil plant (O. americanum L.) decreased after being given drought stress at a field capacity level of 25%, while the SOD content of basil (O. americanum L.) did not differ significantly but increased with the increase in dolomite doses and field capacity given.
Effect of Mercury Stress on the Growth and Lipid Content of Euglena sp. and Echinodorus palaefolius Wisnu Eka Wardana; Dea Hastaning Tantri; Marshanda Nur Roosyana Afifah; Hasna Nur Aini; Dwi Umi Siswanti; Khusnul Qonita Maghfiroh; Tia Erfianti; Ria Amelia; Dedy Kurnianto; Eko Agus Suyono
Jurnal Biodjati Vol 8 No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.23764

Abstract

One way to reduce the adverse effects of the heavy metals mercury in the aquatic environment are using organisms to break down or convert toxic substances into non-toxic forms, either by phytore- mediation or phycoremediation. This research aimed to analyze the growth and lipid content of Euglena sp. after mercury exposure. This research also aimed to analyze the growth of E. palaefolius which is associated with Euglena sp. In this study, the bioremediation ability of Euglena sp. and Echinodorus palaefolius through treatment with mercury concentrations of 5 ppm, 10 ppm, 15 ppm, and 20 ppm, as well as association and non-association treatments. The parameters are the growth of Euglena sp. and the association between Euglena sp. andE. palaefolius measurement and lipid content. The result of the growth of Euglena sp. experienced a significant increase. Lipid content in Euglena sp. was also seen high at 10 ppm mercury concentration. In E. palaefolius, the ability to adsorb heavy metals was also shown by the large diameter of the stems and also the plant growth which has  optimal growth in the treatment of 10 ppm mercury stress.
Co-Authors Akrima Syahidah Alam, Lucky Prabowo Miftachul Ananmaimuna, Jovika Syifa Anindita Della Rosa Riyadi Aprida Niken Arianda Poetri Shofia Rochman Azkiya, Nala Azzizah, Rikka Nur BUDI SETIADI DARYONO Cici Darsih Dea Hastaning Tantri Dedy Kurnianto Diah Rachmawati Eko Agus Suyono Eko Agus Suyono Erfianti, Tia Fitria Yulia Rachmawati Hamdian Hamdian Hardianto, Muhammad Andhi Hari Purwanto Hasna Fatin Affifah Hasna Nur Aini Himawan Tri Bayu Murti Petrus Khusnul Qonita Maghfiroh Kurnianto, Dedy Lena Mardiana Lestari, Nurrisma Ika Maghfiroh, Khusnul Qonita Malika, Pradhika Cikal Marlyn Dian Laksitorini, Marlyn Dian Marshanda Nur Roosyana Afifah Maryani Maryani Melinda Fajar Lestari Melinda Fajar Lestari Mifta Arlinda Nadhifa Athaya Khairunnisa Niken Wulansari Nilamsari, Regina Nindy Senissia Asri Nur Hidayah Pangestuti Nurafifah, Istini Nurhanifah, Tsurayya Okky Shavira Riesty Palupi, Dwi Rekno Putri, Renata Adaranyssa Egistha Rahmasari, Firdaus Salvia Rain, Oryza Enwiera Rega Virgiyana Agustin Rega Virgiyana Agustin Rega Virgiyana Agustin Ria Amelia Sadewo, Brilian Ryan Sahid, Muhammad Novrizal Abdi Sinambela, Benn Gerson Juan Moreno Soenarwan Hery Poerwanto, Soenarwan Hery Solly Aryza Sudjino, Sudjino Syahidah, Akrima Tia Erfianti Untari, Ludmilla Fitri Utami, Indrawati Dian Utaminingsih Utaminingsih Vidhantyka, K.H. Mahadhevy Ryrathna Wisnu Eka Wardana