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All Journal AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Rohimatun Rohimatun, Rohimatun
Balai Penelitian Tanaman Rempah dan Obat
Agriculture, Biological Sciences & Forestry

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Molecular identification and characterization of Maconellicoccus multipori (Takahashi) (Hemiptera: Pseudococcidae) on Piper nigrum L. Mardiningsih, Tri Lestari; Sartiami, Dewi; Miftakhurohmah, Miftakhurohmah; Djiwanti, Setyowati Retno; Rohimatun, Rohimatun; Hardiyanti, Siti; Heryanto, Rubi; Amaria, Widi; Suwarti, Suwarti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225201-207

Abstract

The mealybug Maconellicoccus multipori (Takahashi) was identified on black pepper (Piper nigrum L.) seedlings in a greenhouse in Bogor, West Java, using both molecular and morphological characterization. Two mealybug isolates were successfully amplified with primer pairs targeting the LCO region of the mitochondrial cytochrome oxidase I (COI) gene, yielding a 491 bp PCR product. The nucleotide sequences of both isolates (GenBank accession numbers LC666906 and LC666907) were identical. Phylogenetic analysis revealed that the Bogor isolates clustered closely with M. multipori populations from China and Thailand, with high sequence homology of 99.30% and 99.10%, respectively. Morphological observations of the adult female specimens further confirmed their identity as M. multipori, based on key diagnostic features including body size, antennal segmentation, cerarii pattern, and distribution of pores and ducts, which correspond to descriptions in established taxonomic keys. This study provides the first molecular characterization of M. multipori in Indonesia. The COI sequence data obtained enhances reference databases for DNA barcoding and strengthens early detection strategies for pest monitoring. These findings are crucial for supporting quarantine inspections, management, and control of M. multipori in black pepper nurseries and preventing its spread to other crops.
Efficacy of Three Variants of Dioscorea bulbifera L. Leaf Extract as Termiticides to Control the Dry Wood Termites (Cryptotermes cynocephalus Light) Oksari, Ade Ayu; Faridha, Faridha; Susanty, Devy; Rizki, Fathan Hadyan; Rohimatun, Rohimatun; Wanda, Irvan Fadli; Arinana, Arinana; Dadang, Dadang
AGRIVITA Journal of Agricultural Science Vol 47, No 3 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i3.4208

Abstract

The leaves of Dioscorea bulbifera L. have chemical compounds that have the potential as bioinsecticides. This study aims to determine the effectiveness of polar, semi-polar, and non-polar fractions in D. bulbifera leaf extract as bioinsecticides. The research design used was a Factorial, Completely Randomized Design consisting of two factors with five repetitions using the contact method against Cryptotermes cynocephalus. The first factor was the type of solvent: non-polar solvents, semi-polar solvents, and polar solvents. The second factor was concentration, with a range of 5%, 4%, 3%, 2%, and 1%. Based on the results, the D. bulbifera leaves in the polar fraction contain alkaloids, flavonoids, phenols, triterpenoids, and saponins. The semi-polar fraction contains alkaloids, phenols, and steroids. The non-polar fraction contains alkaloids, phenols, and triterpenoids. GC-MS identification showed 20 metabolite compounds as potential as botanical insecticides. The highest termite mortality was in the non-polar fraction, with a 4% concentration of 32.80% (equivalent to a corrected mortality of 27.58%). The semi-polar fraction (3.20 g and 15.10%) at a concentration of 5% (2.58 g and 12.19%), showed the best reduction in test weight and degree of damage. This result indicates that D. bulbifera is an effective bioinsecticide in the repellent group.
Co-Authors Ade Ayu Oksari Arinana Arinana DADANG DADANG Devy Susanty DEWI SARTIAMI Faridha, Faridha Heryanto, Rubi Irvan Fadli Wanda MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH Rizki, Fathan Hadyan SETYOWATI RETNO DJIWANTI, SETYOWATI RETNO Siti Hardiyanti Suwarti Suwarti Tri Lestari Mardiningsih, Tri Lestari Widi Amaria