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All Journal AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Media Pertanian
Rohimatun Rohimatun, Rohimatun
Balai Penelitian Tanaman Rempah dan Obat
Agriculture, Biological Sciences & Forestry

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Molecular identification and characterization of Maconellicoccus multipori (Takahashi) (Hemiptera: Pseudococcidae) on Piper nigrum L. Mardiningsih, Tri Lestari; Sartiami, Dewi; Miftakhurohmah, Miftakhurohmah; Djiwanti, Setyowati Retno; Rohimatun, Rohimatun; Hardiyanti, Siti; Heryanto, Rubi; Amaria, Widi; Suwarti, Suwarti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225201-207

Abstract

The mealybug Maconellicoccus multipori (Takahashi) was identified on black pepper (Piper nigrum L.) seedlings in a greenhouse in Bogor, West Java, using both molecular and morphological characterization. Two mealybug isolates were successfully amplified with primer pairs targeting the LCO region of the mitochondrial cytochrome oxidase I (COI) gene, yielding a 491 bp PCR product. The nucleotide sequences of both isolates (GenBank accession numbers LC666906 and LC666907) were identical. Phylogenetic analysis revealed that the Bogor isolates clustered closely with M. multipori populations from China and Thailand, with high sequence homology of 99.30% and 99.10%, respectively. Morphological observations of the adult female specimens further confirmed their identity as M. multipori, based on key diagnostic features including body size, antennal segmentation, cerarii pattern, and distribution of pores and ducts, which correspond to descriptions in established taxonomic keys. This study provides the first molecular characterization of M. multipori in Indonesia. The COI sequence data obtained enhances reference databases for DNA barcoding and strengthens early detection strategies for pest monitoring. These findings are crucial for supporting quarantine inspections, management, and control of M. multipori in black pepper nurseries and preventing its spread to other crops.
Efficacy of Three Variants of Dioscorea bulbifera L. Leaf Extract as Termiticides to Control the Dry Wood Termites (Cryptotermes cynocephalus Light) Oksari, Ade Ayu; Faridha, Faridha; Susanty, Devy; Rizki, Fathan Hadyan; Rohimatun, Rohimatun; Wanda, Irvan Fadli; Arinana, Arinana; Dadang, Dadang
AGRIVITA Journal of Agricultural Science Vol 47, No 3 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i3.4208

Abstract

The leaves of Dioscorea bulbifera L. have chemical compounds that have the potential as bioinsecticides. This study aims to determine the effectiveness of polar, semi-polar, and non-polar fractions in D. bulbifera leaf extract as bioinsecticides. The research design used was a Factorial, Completely Randomized Design consisting of two factors with five repetitions using the contact method against Cryptotermes cynocephalus. The first factor was the type of solvent: non-polar solvents, semi-polar solvents, and polar solvents. The second factor was concentration, with a range of 5%, 4%, 3%, 2%, and 1%. Based on the results, the D. bulbifera leaves in the polar fraction contain alkaloids, flavonoids, phenols, triterpenoids, and saponins. The semi-polar fraction contains alkaloids, phenols, and steroids. The non-polar fraction contains alkaloids, phenols, and triterpenoids. GC-MS identification showed 20 metabolite compounds as potential as botanical insecticides. The highest termite mortality was in the non-polar fraction, with a 4% concentration of 32.80% (equivalent to a corrected mortality of 27.58%). The semi-polar fraction (3.20 g and 15.10%) at a concentration of 5% (2.58 g and 12.19%), showed the best reduction in test weight and degree of damage. This result indicates that D. bulbifera is an effective bioinsecticide in the repellent group.
Keanekaragaman Artropoda pada Berbagai Kombinasi Pola Tanam Jagung Ketan dan Edamame Aisyah, Mahindra Dewi Nur; Kurniawan, Bayu; Erdiansyah, Iqbal; Alif, Trisnani; Soelaksini, Liliek Dwi; Irawan, Triono Bambang; Widodo, Tirto Wahyu; Rohimatun, Rohimatun
Media Pertanian Vol 10, No 2 (2025): Media Pertanian
Publisher : Program Studi Agroteknologi Universitas Siliwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37058/mp.v10i2.16570

Abstract

Sistem tanam monokultur yang umum diterapkan petani cenderung menurunkan kestabilan agroekosistem karena meningkatnya risiko serangan hama dan penyakit. Salah satu alternatifnya ialah sistem polikultur yang dapat memperkaya habitat bagi berbagai jenis artropoda, termasuk musuh alami hama. Penelitian ini bertujuan untuk menganalisis keanekaragaman artropoda pada berbagai kombinasi tanam jagung ketan dan kedelai edamame dengan tambahan tanaman border serai dan kemangi. Penelitian dilaksanakan pada empat tipe lahan pertaniandi Kebonsari, Jember, Jawa Timur: 1) monokultur jagung ketan, 2) polikultur jagung ketan-edamame, (3) polikultur jagung ketan-edamame dengan border serai, dan 4) polikultur jagung ketan-edamame dengan border kemangi. Pengambilan sampel dilakukan menggunakan pitfall trap, yellow trap, dan sweep net, sedangkan data dianalisis menggunakan indeks keanekaragaman (H'), kemerataan (E), dan dominasi (C). Hasil menunjukkan bahwa perlakuan polikultur bersamaan dengan border tanaman aromatik terbukti meningkatkan keseimbangan komunitas arthropoda yang menguntungkan. Polikultur kemangi efektif meningkatkan parasitoid, sedangkan polikultur serai lebih optimal dalam meningkatkan predator dan penyerbuk.  The monoculture system commonly practiced by Indonesian farmers tends to reduce agroecosystem stability due to the higher risk of pest and disease. On the other hand, polyculture can provide a more diverse habitat that supports beneficial arthropods, including natural enemies. This study aimed to analyze the diversity of arthropods in various planting combinations of waxy maize and edamame with additional border plants of lemongrass and basil. The research was conducted from June to September 2024 in Kebonsari, Jember, East Java, using four treatments: (1) maize monoculture, (2) maize–edamame polyculture, (3) maize–edamame polyculture with lemongrass borders, and (4) maize–edamame polyculture with basil borders. Arthropod sampling was carried out using pitfall traps, yellow traps, and sweep nets, and data were analyzed using the diversity index (H'), evenness index (E), and dominance index (C). The results showed that the polyculture combined with aromatic plants as a border effectively enhanced the balance of beneficial arthropod communities. Polyculture with basil effective in increasing parasitoid abundance, while polyculture with lemongrass is more optimal in increasing predators and pollinators. 
Co-Authors Ade Ayu Oksari Aisyah, Mahindra Dewi Nur Alif, Trisnani Arinana Arinana Bayu Kurniawan DADANG DADANG Devy Susanty DEWI SARTIAMI Faridha, Faridha Heryanto, Rubi Irawan, Triono Bambang Irvan Fadli Wanda MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH Rizki, Fathan Hadyan SETYOWATI RETNO DJIWANTI, SETYOWATI RETNO Siti Hardiyanti Soelaksini, Liliek Dwi Suwarti Suwarti Tri Lestari Mardiningsih, Tri Lestari Widi Amaria Widodo, Tirto Wahyu