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All Journal Journal of Tropical Soils Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Wahana Didaktika Prosiding Universitas PGRI Palembang BERITA BIOLOGI Journal of Tropical Crop Science PROSIDING SEMINAR NASIONAL PROGRAM PASCASARJANA JMIE (Journal of Madrasah Ibtidaiyah Education) Agro Bali: Agricultural Journal jurnal dikdas bantara Journal Of Human And Education (JAHE) Jurnal Ilmiah Pendidikan Dasar (JIPDAS) Innovative: Journal Of Social Science Research Jurnal Abdimas Cendekiawan Indonesia
Etty Pratiwi, Etty
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Nursing Public Health Social Sciences Other

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Journal : BERITA BIOLOGI

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PROTEIN TOKSININSEKTISIDAL DARI BAKTERI PATOGEN SERANGGA Photorhabdus luminescens HJ Akhdiya, Alina; Pratiwi, Etty; Samudra, I Made
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.996 KB) | DOI: 10.14203/beritabiologi.v8i6.828

Abstract

Photorhabdus luminescens HJ is an entomopathogenic bacterium that has a high toxicity against Tenebrio molitor larvae.Toxicity assay of crude extra cellular protein precipitated using ammonium sulphate showed that the highest toxin activity was found in 70 % saturation. Purification of the toxin using Hi Prep 16/60 Sephacryl S-200 HR column exhibited one fraction of toxic protein and three fractions of non-toxic protein. Mortality of T. molitor larvae treated with 19.2 nanogram of toxic fraction was up to 80%. Denatured protein analysis using sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed that the toxic fraction was composed of three proteins, which were 19.5, 42, and 66 kDa respectively. Based on toxin activity bioassay, this toxin type was an injectable toxin and presumably classified as Mcf toxin.
Co-Authors Akbar Zam, M. Ali ALINA AKHDIYA Amanatul Muslimah, Zulfa Amiruddin Amiruddin Aswadi Jaya, Aswadi Atika Sari, Jelita Bambang Sapta Purwoko Dewi Kartika Sari Dewi Kartika Sari, Dewi Dian Maharani, Dian Dwistya, Yucha Effendi, Darwin Evi Rosmiyati, Evi Fahrizal Hazra Faqih Udin Ferri Hidayad, Ferri Fitriana Mardhotillah, Annisa Hatim, Magdad Hermansyah Hermansyah I Made Samudra INDRAWATI, SRI WAHYU Magana, Richard Elisha Malik, Nur Fitri Qhoirunnisa Abdul Maya Melati Munif Ghulamahdi Ninin Non Ayu Salmah, Ninin Non Ayu Noviati Noviati Noviati, Hj. Padilah, Padilah Patricia Lubis, Patricia Rachmawati, Diana Widhi Riyanto Riyanto ROHANA ROHANA Theriana, Ana Ubad Badrudin Winanda, Tari Yusanti, Indah Anggraini