Article Per Year (5 Year)
p-Index From 2021 - 2026
1.756
P-Index
This Author published in this journals
All Journal Majalah Obat Tradisional Jurnal Bioteknologi & Biosains Indonesia (JBBI) Biosaintifika: Journal of Biology & Biology Education BioEksakta : Jurnal Ilmiah Biologi Unsoed Journal of Science and Technology Research for Pharmacy Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Nuraeni Ekowati, Nuraeni
Faculty of Biology, University of Jenderal Soedirman, Purwokerto, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Published : 22 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 22 Documents
Search

 1   2   3 
Karakterisasi Isolat Khamir Gf1 Terhadap Kondisi Asam Saluran Pencernaan Unggas Ainillah, Awwaluz Zahroh Mahya; Ekowati, Nuraeni; Suryani, Ade Erma
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.1.1745

Abstract

Probiotik merupakan mikroorganisme yang dapat memperbaiki mikroekologi usus yang berdampak positif terhadap kesehatan inang. Kandidat probiotik merupakan mikroorganisme yang memiliki potensi sebagai probiotik, seperti menghasilkan enzim fitase sehingga mampu mengoptimalkan kondisi saluran pencernaan. Salah satu mikroorganisme sebagai kandidat probiotik adalah khamir penghasil enzim fitase. Khamir penghasil fitase telah diisolasi oleh BPTBA-LIPI Yogyakarta asal makanan fermentasi tradisional Indonesia yaitu gathot.Pengujian khamir fitase sebagai kandidat probiotik dilakukan dengan menstimulasikan kondisi saluran pencernaan seperti kondisi asam yaitu pada pH rendah dan kondisi asam lambung. Kondisi pH yang rendah dan asam lambungmerupakan suatu penghalang masuknya mikroorganisme ke saluran gastrointestinal, oleh karena itu pengujian terhadap asam dilakukan untuk mengetahui karakteristik mikroorganisme yang akan digunakan sebagai kandidat probiotik. Pengujian dilakukan secara invitro dengan menguji ketahanan khamir pada kondisi asam dengan nilai pH 2 dan 3 serta kondisi asam lambung dengan menggunakan pepsin 0,3%. Hasil penelitian menunjukkan bahwa isolat khamir GF1 asal makanan tradisional fermentasi mampu tahan terhadap kondisi asam karena mampu mempertahankan viabilitasnya pada pH 3 dan kondisi asam lambung.
Aplikasi Jamur Pelapuk Putih pada metode Biopulping berbahan dasar Limbah Daun Kayu Putih (Melaleuca leucadendron) Mubarrak, Syifa; Mumpuni, Aris; Ekowati, Nuraeni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 4 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3386

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh jamur pelapuk putih (JPP) pada kadar lignin dan selulosa dalam proses biopulping limbah daun kayu putih lama dan limbah daun kayu putih baru dan mengetahui JPP terbaik untuk proses biopulping limbah daun kayu putih. Penelitian menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL). Perlakuan berupa menginokulasikan JPP (Phanerochaete chrysosporium, Ganoderma lucidum, dan Pleurotus tuberregium), tiap perlakuan diulang sebanyak 3 kali. Variabel penelitian terdiri atas variabel bebas berupa jenis JPP yang berbeda dan umur limbah, variabel terikat berupa kemampuan biodelignifikasi JPP pada limbah daun kayu putih. Parameter utama yang diamati adalah kadar lignin dan kadar selulosa sebelum dan sesudah masa inkubasi daun kayu putih. Parameter pendukung yang diamati adalah berat limbah sebelum dan sesudah inkubasi, pH, dan Berat Media.Hasil penelitian menunjukkan bahwa terjadi penurunan kadar lignin dan selulosa pada seluruh perlakuan. Hasil rata-rata penurunan kadar lignin terbesar yakni pada perlakuan jamur P. tuberregium terhadap limbah daun kayu putih lama sebesar 0,014%. Penurunan kadar selulosa akhir tertinggi pada perlakuan jamur terbesar adalah pada jamur G. lucidum terhadap limbah daun kayu putih lama mengalami rata-rata penurunan selulosa terbesar 3,22%.
POTENSI EKSTRAK ETIL ASETAT Coprinus comatus TERHADAP KADAR SGOT DAN SGPT PADA TIKUS PUTIH MODEL DIABETES Feryawan, Feryawan; Ratnaningtyas, Nuniek Ina; Ekowati, Nuraeni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4238

Abstract

Coprinus comatus atau jamur paha ayam mengandung senyawa bioaktif antara lain flavonoid, terpenoid, comatin, tokoferol, dan ergotionin yang berfungsi sebagai antioksidan dan antidiabetes. Diabetes mellitus merupakan penyakit kronis yang berkaitan dengan gangguan sekresi insulin atau menurunnya sensitivitas insulin yang menyebabkan kadar glukosa dalam darah meningkat melebihi normal (hiperglikemia). Hiperglikemia dapat menyebabkan stres oksidatif dan meningkatkan Reactive Oxygen Species (ROS) sehingga memacu terjadinya peroksidasi lipid yang merusak pankreas, ginjal, dan hati. Salah satu cara untuk mengetahui kerusakan hati dengan mengukur kadar enzim hati Serum Glutamat Oksaloasetat Transaminase (SGOT) dan Serum Glutamat Piruvat Transaminase (SGPT) yang meningkat dalam darah. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian dan menentukan berapakah dosis yang efektif ekstrak etil asetat C. comatus dengan dosis yang berbeda terhadap kadar SGOT dan SGPT dalam darah tikus putih model diabetes. Hasil penelitian menunjukkan bahwa pemberian ekstrak etil asetat C. comatus dapat menurunkan kadar SGOT dan SGPT serta kadar glukosa darah tikus putih yang diinduksi streptozotocin. Ekstrak etil asetat C. comatus dosis 500 mg/kg BB merupakan dosis yang paling efektif dalam menurunkan kadar SGOT dan SGPT dalam darah tikus yang diinduksi streptozotocin dengan rerata kadar SGOT sebesar 57,96 ± 1,58 U/L dan SGPT sebesar 29,67 ± 3,56 U/L, serta presentase penurunan kadar SGOT dan SGPT yang dibandingkan kontrol negatif masing-masing sebesar 43,15% dan 68,70%, serta glukosa darah sebesar 19,62%. Kata kunci : Coprinus comatus, diabetes mellitus, SGOT, SGPT
Detection of Psychotropic Compound in Coprophilous Fungi in District of Baturraden Banyumas Regency Amanda, Safira; Mumpuni, Aris; Ekowati, Nuraeni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.4245

Abstract

Coprophilous fungi or dung-loving fungi are fungal group which adapted to life on dung and fecal of herbivorous animals. Coprophilous fungi contain psychotropic compounds, substances or drugs, both natural and synthetic, but not narcotics. This compound comprises psychoactive properties that cause selective influence on the central nervous system and lead distinctive changes in mental activity and behavior. Chemical Spot Test still remain an important method for the preliminary identification of illicit drugs and other psychotropic compound in spite of developments in instrumental technology which enables to use in field. Banyumas Regency, especially Baturraden district is a very potential habitat for coprophilous fungi due to a lot of cattle farms were established there. In addition, the environmental condition also suitable for the fungal growth. This study aim to make an inventory and identify the coprophilous fungi that found in District of Baturraden Banyumas Regency and detect the presence of psychotropic compound in that fungi. The research will be conducted using purposive random sampling and Color Test or Chemical Spot Test analysis. The obtained data is analyzed descriptively by comparing with Atlas of The Munsell Color System. This research obtained seven genera of coprophilous fungi i.e: Coprinellus sp., Coprinopsis sp., Entoloma sp., Gymnopus sp., Lepiota sp., Parasola sp. and Stropharia sp. that discovered in two cattle farms in Baturraden District. Key Words: coprophilous, fungi, psychotropic
Pengaruh pH dan Waktu Inkubasi Berbeda Terhadap Pertumbuhan dan Produksi β-Glukan Schizophyllum commune Maharani, Amalia Sofia; Ekowati, Nuraeni; Ratnaningtyas, Nuniek Ina
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4594

Abstract

Schizophyllum commune is a fungus that grows naturally on tree trunks and wood processing waste. This mushroom contains β-glucan compounds which have the potential for drug development in the world of health. The main purpose of this study was to determine the optimum value of pH and incubation time on S. commune's growth, and also the optimum value of pH and incubation time on β-glucan production of S. commune. The research was conducted by experimental method of completely randomized factorial design (CRD factorial). The treatment given includes variations in pH (P) with three levels, namely pH 5 (P1), pH 6 (P2), and pH 7 (P3), and variations in incubation time (W) with three levels, namely incubation time of 20 days (W1), 25 days (W2), and 30 days (W3). . The independent variables were pH and incubation time, while the dependent variables were fungal mycelium growth and β-glucan production. The main parameter observed was β-glucan weight. The supporting parameters were dry biomass weight and the final pH medium. Data analysis was performed by Analysis of Variance (ANOVA) at 95% accuracy levels, followed by Duncan's test (Ducan Multiple Range Test). The results showed that pH and incubation time were significantly affected to the growth and production of β-glucan fungus S. commune. The value of pH 6 and incubation time of 25 days was the optimum condition for the growth of S. commune, and also pH 5 and incubation time of 25 days was the optimum condition for β-glucan production of S. commune.
Optimasi Konsentrasi Glukosa dan Waktu Inkubasi Terhadap Bobot β-Glukan Jamur Schizophyllum commune Apriyani, Tri Rahayu; Ekowati, Nuraeni; Ratnaningtyas, Nuniek Ina
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4601

Abstract

Schizophyllum commune is a fungus that grows wild in nature which contains β-glucan which has the potential to develop drugs for several diseases and human health. The main purpose of this study was to determine the effect glucose concentration and incubation time on the growth of fungus S. commune and its β-glucan production. The research was conducted by experimental method of completely randomized factorial design (CRD factorial) with two factors carried out in three repetitions. The treatments provided included variations in glucose concentration (G) with three variations, namely 10 g/L (G1), 20 g/L (G2), and 30 g/L (G3), and variations in three different incubation times (W), such as 20 days (I1). , incubation time of 25 days (I2), and incubation time of 30 days (I3). Variables which used in this research independent and dependent variables. The independent variables were glucose concentration and incubation time, while the dependent variables were fungal mycelium growth and β-glucan production. The main parameter observed was β-glucan weight. The supporting parameters were dry biomass weight and the final pH medium. Data analysis was performed by Analysis of Variance (ANOVA) at 95% accuracy levels, followed by Duncan's test (Ducan Multiple Range Test). The results showed that glucose concentration and incubation time were significantly affected to the growth and production of β-glucan fungus S. commune. Glucose concentration of 30 g/L and incubation time of 25 days was the optimum condition for the growth of S. commune and its β-glucan production with an average dry weight of betaglucan is 0.363 g/L
Deteksi Senyawa Psikotropika pada Jamur Kotoran Sapi di Kecamatan Karanglewas Kabupaten Banyumas hasanah, Amanda rohmatun; Mumpuni, Aris; Ekowati, Nuraeni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 2 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.2.4685

Abstract

Jamur koprofil adalah kelompok jamur yang tumbuh di dalam kotoran hewan herbivora. Beberapa jenis jamur koprofil adalah jamur pangan yang dapat dikembangkan sebagai penyedia protein, dan beberapa di antaranya juga merupakan jamur beracun, terutama yang mengandung senyawa psikotropika yang dapat dimanfaatkan secara positif sebagai bahan pembuatan obat penenang. Penelitian tentang deteksi senyawa psikotropika pada jamur kotoran ternak belum banyak dilakukan. Kondisi lingkungan di Kecamatan Karanglewas ditemukan tumbuhnya jamur koprofil di daerah tersebut. Jamur koprofil berpotensi untuk dimanfaatkan atau disalahgunakan oleh masyarakat, oleh karena itu penelitian ini perlu dilakukan untuk mempelajari keberadaan jamur koprofil dan senyawa psikotropika yang terkandung dalam jamur koprofil. Penelitian ini bertujuan untuk mengidentifikasi jamur koprofil yang tumbuh pada kotoran sapi di Kecamatan Karanglewas Kabupaten Banyumas, dan mendeteksi adanya senyawa psikotropika pada tubuh buah dan miselium jamur koprofil yang diperoleh. Hasil penelitian menunjukkan bahwa diperoleh tujuh genera cendawan koprofil dari kandang sapi di Kecamatan Karanglewas Kabupaten Banyumas yaituCoprinopsis , Mycena , Panaeolus , Inocybe , Ascobolus , Psilocybe , dan Coprinus . Keberadaan senyawa psikotropika baik pada tubuh buah maupun miselium jamur koprofil terdeteksi pada empat genus, yaitu Coprinopsis , Panaeolus , Inocybe , dan Psilocybe .
Pengaruh Jenis dan Kosentrasi Bahan Tambahan terhadap pertumbuhan Jamur Tiram Coklat (Pleurotus pulmonarius) MU'AFA, RAFID HILMY; Ratnaningtyas, Nuniek Ina; Ekowati, Nuraeni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.5022

Abstract

Brown oyster mushroom (Pleurotus pulmonarius) is one type of wood mushroom that can be cultivated, but it is not yet popular for oyster mushroom cultivators and consumers in Indonesia. Planting medium materials give different results, depending on the ratio of the given composition. This study aims to determine the effect of different types and concentrations of additives on the growth of brown oyster mushrooms and to determine the best type and concentration of additives for the growth of chocolate oyster mushrooms. This study used a Factorial Completely Randomized Design (CRD) consisting of the following treatments: type of additives, namely rice bran, corn flour, polar, and wheat flour, as well as different concentration factors of additives, namely 0%, 5%, 10%, 15%, and 20%. Each treatment was repeated 4 times, so there were 80 experimental units. The independent variables in this study were the type and concentration of additives, while the dependent variable was the growth rate of fungal mycelium. The main parameters observed were mycelium growth and fruit body weight of brown oyster mushroom (P. pulmonarius), while the supporting parameters were the appearance of the first fruiting body, mycelium density, incubation room temperature, and pH of the growing medium. The data obtained were analyzed using analysis of variance (ANOVA) with an error rate of 5% and followed by Duncan's Multiple Range Test (DMRT) with an error rate of 5%. The results showed that the interaction between the type and concentration of additives affected the production of brown oyster mushrooms (P < 0.05). The type of rice bran additive with a concentration of 20% showed the best results, namely 0.97 cm/day for mycelium growth rate and 0.885 kg for brown oyster mushroom fruiting body weight.
Optimasi Jenis Jamur Pelapuk Putih dan Bahan Lignoselulosik Limbah Pertanian dalam Pembuatan Biokomposit berbasis Miselium Rahmadianti, Maghfira Rizki; Mumpuni, Aris; Ekowati, Nuraeni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 6 No 1 (2024): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2024.6.1.6636

Abstract

Melimpahnya ketersediaan bahan lignoselulosik berupa limbah ampas tebu dan batang tanaman jagung serta kemampuan jamur pelapuk putih Ceriporia lacerata dan Auricularia auricula mendegradasi lignoselulosik dapat dimanfaatkan sebagai pengembangan biokomposit berbasis miselium. Penelitian ini bertujuan untuk mengetahui pengaruh antara jenis jamur pelapuk putih dengan komposisi bahan lignoselulosik limbah pertanian terhadap kualitas produk biokomposit yang dihasilkan serta mendapatkan jenis jamur pelapuk putih dan jenis substrat bahan lignoselulosik limbah pertanian yang optimal dalam pembuatan biokomposit berbasis miselium. Penelitian ini menggunakan metode experimental Rancangan Acak Lengkap dengan 10 perlakuan dan 3 kali ulangan. Variabel penelitian terdiri dari variabel bebas dan variabel terikat. Variabel bebas berupa jenis bahan lignoselulosik limbah pertanian dan jenis jamur pelapuk putih, sedangkan variabel terikat yaitu kualitas biokomposit berbasis miselium yang dihasilkan. Parameter yang diukur dalam penelitian ini yaitu parameter utama dan parameter pendukung. Parameter utama yaitu pertumbuhan jamur pada bahan komposit, kerapatan komposit, kadar air, pengembangan tebal komposit, modulus elastisitas dan modulus patah komposit. Parameter pendukungnya berupa biodegradabilitas komposit. Hasil penelitian menunjukkan bahwa jamur pelapuk putih dan komposisi bahan lignoselulosik yang berbeda berpengaruh secara nyata terhadap kualitas biokomposit yang dihasilkan kecuali pada parameter kadar air dan modulus patah. Hasil penelitian untuk parameter pertumbuhan miselium, kerapatan komposit, kadar air, pengembangan tebal, modulus elastisitas, dan modulus patah secara berturut-berturut berkisar antara 9,38-18,49 mm/hari, 0,11-0,21 g/cm3, 8,34%-16,19%, 3,01%-10,93%, 1188,33-14873,33 MPa, dan 1,76-25,11 MPa. Berdasarkan hasil skoring biokomposit yang dilakukan, perlakuan C. lacerata dengan komposisi pada ampas tebu 100% menghasilkan kualitas biokomposit terbaik.
Utilization of Pleurotus ostreatus And Lentinus squarrosulus In The Manufacture of Mycelium-Based Biocomposite Using Sugarcane Bagasse And Cornstalk Media Wardaya, Yasenia Sandra; Mumpuni, Aris; Ekowati, Nuraeni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 2 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.2.6669

Abstract

Biocomposite is a type of composite consisting of a polymer matrix material and natural fiber reinforcement. Biocomposite technology, especially natural fibers, is currently in demand. The reasons are environmentally friendly, availability of many raw materials, low production costs, biodegradable, and recyclable. Natural fibers used in the form of agricultural waste such as sugarcane bagasse and corn stalks while the mycelium of the fungus Pleurotus ostreatus and Lentinus squarrosulus are used as natural adhesives. The objectives of this research were to determine the effect of the type of white-rot fungus and the composition of the lignocellulosic material of agricultural waste that affects the quality of the resulting biocomposite board and to obtain the optimal type of white-rot fungus and the optimal composition of lignocellulosic material from agricultural waste to manufacture mycelium-based biocomposite board. The research method used a completely randomized design with ten treatments with three replications. The treatments used 2 types of mushroom (P. ostreatus and L. squarrosulus) with 2 types of Lignocellulosic materials (Sugarcane bagasse and Cornstalk) and each lignocellulosic material has 4 types of compositions (100%, 75%, 50%, and 25%). The main parameter was analyzed using Analysis of Variance (ANOVA) with an error rate of 5%, then further tested with post hoc Duncan at 95% confidence level to compare the effect between treatments. The results showed that the treatment of the type of fungus and the lignocellulosic material used affects the quality of the resulting biocomposite board. L. squarrosulus in 100% sugarcane bagasse media is the optimal type of white-rot fungus and the optimal composition of lignocellulosic material to manufacture mycelium-based biocomposite board.
Co-Authors . HERNAYANTI Abd. Rasyid Syamsuri Ade Erma Suryani, Ade Erma Ainillah, Awwaluz Zahroh Mahya Aisyah Tri Septiana Amanda, Safira Amelia, Aurora Pradipta Andarwanti, Suci Apriyani, Tri Rahayu Aris Mumpuni C.J. SOEGIHARDJO Dalia Sukmawati Endang Sri Purwanti Endang Sri Purwati Feryawan, Feryawan hasanah, Amanda rohmatun I Gusti Wayan Murjana Yasa Juni Safitri Muljowati Maharani, Amalia Sofia MU'AFA, RAFID HILMY Mubarrak, Syifa Nuniek Ina Ratnaningtyas Nursamsi Pusposendjojo, Nursamsi Purnomowati Purnomowati, Purnomowati Rahmadianti, Maghfira Rizki Ratnaningtyas, ‪Nuniek Ina Rina Sri Kasiamdari Supriyadi, Adi Triyadi Hendra Wijaya Wardaya, Yasenia Sandra Warsinah Warsinah Yuniati, Nilasari Indah