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THE EFFECT SODIUM HYPOCHLORITE 2,5% AND SODIUM THIOSULPHATE AS IRRIGATION ON THE CLEANLINESS OF THE APICAL THIRD ROOT Christina Mahardika; Yulita Kristanti; Margareta Rinastiti
ODONTO : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.1.81-87

Background: Sodium hypochlorite produces free oxygen and free radicals that cause damage to dentin collagen. Combination with Sodium thiosulfate with the aim of improving root canal hygiene and restoring the oxidizing effect on the dentin surface.The apical third of the root canal is the most difficult part, due to its narrower anatomy, curvature and frequent branching of the root canal. Method: The study used 30 specimens which were divided into 3 groups, group I was irrigated with 2.5% NaOCl, 5% sodium thiosulfate and saline, group II was irrigated with 2.5% NaOCl, 10% sodium thiosulfate and saline and group III was irrigated with 2.5% NaOCl and saline. The tooth length 14 mm measured from apical to crown. The crown down technique was prepared using the M3 Pro gold file. Observation of the level of cleanliness of the root canal was carried out using a Scanning Electron Microscope (SEM) with 5000x magnification at apical third . The results of micrographic photographs are then given a score of 1-4.Result: Irrigated with 5% and 10% sodium thiosulfate showed no significant difference.Conclusion: The differences is not significant, 2,5% irrigation with sodium thiosulfate resulted in a higher cleanliness apical third of the root canalthan 2,5% NaOCl irigation with saline

Replantasi intensional fraktur gigi vertikal menggunakan mineral trioxide aggregate dan self adhesive resin cement terhadap pembentukan kolagen tipe I Tri Endra Untara; Yulita Kristanti; Andina Widyastuti
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 8, No 2 (2022)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.81868

Replantasi intensional merupakan salah satu cara untuk mempertahankan gigi yang mengalami fraktur vertikal. Replantasi gigi dengan fraktur vertikal memerlukan kerapatan pada sisi fraktur dengan baik. Hal ini dapat dicapai dengan cara penempatan perekat fragmen fraktur yang tepat yang dapat diterima tubuh agar dicapai penyembuhan yang baik. Tujuan penelitian ini adalah untuk mengetahui reaksi regenerasi jaringan periradikuler dengan indikator pembentukan kolagen tipe I pada penggunaan self-adhesive resin cement dan mineral trioxide aggregate (MTA) sebagai bahan penutup garis fraktur. Penelitian menggunakan 27 ekor kelinci New Zealand jantan yang dibagi menjadi 3 kelompok perlakuan. Kelompok I tanpa aplikasi bahan (kontrol), kelompok II dengan aplikasi MTA dan kelompok III dengan aplikasi self-adhesive resin cement. Pengamatan pembentukan kolagen tipe I dilakukan pada hari ke-7 (minggu I), hari ke-14 (minggu II) dan hari ke-21 (minggu III). Serum diambil dari darah kelinci melalui vena auricularis. Kadar kolagen tipe I diamati dengan rabbit collagen type I kit menggunakan teknik ELISA. Data dianalisis dengan analisis variansi dan post hoc LSD dengan tingkat kepercayaan 95%. Hasil uji statistik dengan analisis variansi menunjukkan bahwa terdapat pengaruh yang signifikan (p < 0,05) penggunaan self-adhesive resin cement dan MTA sebagai penutup garis fraktur pada replantasi intensional fraktur gigi vertikal terhadap pembentukan kolagen tipe I. Pembentukan kolagen tipe I kelompok dengan aplikasi MTA lebih tinggi dari kelompok kontrol maupun kelompok self-adhesive resin cement pada pengamatan minggu II dan minggu III (p < 0,05). Berdasarkan penelitian yang telah dilakukan dapat diambil kesimpulan bahwa pembentukan kolagen tipe I pada aplikasi MTA lebih tinggi daripada self-adhesive resin cement.

RETRIEVING BROKEN INSTRUMENT IN HALF OF THE APIKAL ON CANINES TEETH WITH H-FILE BRAIDING TECHNIQUE Yunita Styaningrum; Yoanita Dwi Andina; Yulita Kristanti
Odonto : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

ABSTRACTBackground: Broken instruments affect the outcome and prognosis. A broken instrument will prevent the procedure of cleaning and shaping, irrigation and obturation of the root canal on the obstructed root section causing failure in endodontic treatment. There are some treatments for broken instrument including retrieving a broken fragment file and bypassing it on the root canal. the aim of the case report aims to provide an alternative for retrieving broken instruments using the H-file braiding technique. Method: A 38-year-old female patient came to the Dental Conservation Clinic RSGM Prof. Soedomo FKG UGM. A patient got a history of initial root canal treatment in a clinic, which ended with a separated file on canine teeth left upper. No complain pain, from the investigation radiographic there is an instrument broken in half apical. The teeth 23 seen temporary restoration cavity class I is still in good. Retrieving broken instruments using the H-file braiding technique, preparation with step back techniques, obturation with warm vertical condensation technique and class I composite resin restorations with fiber-reinforced retention. Result: No complain pain after 2 weeks retrieving broken instrument.Conclusion: The technique of the H-file braiding technique is one technique to retrieve a broken instrument, this procedure is simple, cost-effective, harmless to the teeth and gives root canal sealing.

APEXIFICATION FOLLOWED BY DIRECT COMPOSITE VENEER ON ELLIS CLASS IV FRACTURE: A CASE REPORT Effendi Halim; Yoanita Dwiaidina; Wignyo Hadriyanto; Pribadi Santosa; Ema Mulyawati; Yulita Kristanti
Odonto : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Introduction: Traumatized immature permanent teeth can cause pulp necrosis, leading to imperfect root formation, and thus resulting an open apex. The apical opening of the immature teeth is mostly large and thus may lead to difficulties in performing a root filling. In such cases, apexification is mandatory before doing root canal treatment. The aim of this case report was to inform that apexification and direct veneer was suitable to treat Ellis class IV fracture with necrotic pulpCase Report: 21-year-old female came to the Dental Conservation Clinic RSGM Prof. Soedomo Faculty of Dentistry UGM with chief complaint of right central incisive maxilla fracture and tooth discoloration. Radiographic examination showed an open apex of tooth 11 with a diffuse apical radiolucent area. Root canal treatment and apexification were carried out using circumferential technique and Mineral Trioxide Aggregate (MTA). Vertical warm obturation was performed, followed by intra coronal bleaching in the next visit. Final restoration was carried out using direct composite veneer. Post treatment radiographic showed a 1 mm diameter of radiolucent area at the apex in a good healing process.Conclusion: Apexification using MTA can be used to treat an open apex tooth. 1Student of Specialist Programme in Conservative Dentistry, Dentistry Faculty, Gadjah Mada University 2Department of Conservative Dentistry, Dentistry Faculty, Gadjah Mada University

THE EFFECT SODIUM HYPOCHLORITE 2,5% AND SODIUM THIOSULPHATE AS IRRIGATION ON THE CLEANLINESS OF THE APICAL THIRD ROOT Christina Mahardika; Yulita Kristanti; Margareta Rinastiti
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Sodium hypochlorite produces free oxygen and free radicals that cause damage to dentin collagen. Combination with Sodium thiosulfate with the aim of improving root canal hygiene and restoring the oxidizing effect on the dentin surface.The apical third of the root canal is the most difficult part, due to its narrower anatomy, curvature and frequent branching of the root canal. Method: The study used 30 specimens which were divided into 3 groups, group I was irrigated with 2.5% NaOCl, 5% sodium thiosulfate and saline, group II was irrigated with 2.5% NaOCl, 10% sodium thiosulfate and saline and group III was irrigated with 2.5% NaOCl and saline. The tooth length 14 mm measured from apical to crown. The crown down technique was prepared using the M3 Pro gold file. Observation of the level of cleanliness of the root canal was carried out using a Scanning Electron Microscope (SEM) with 5000x magnification at apical third . The results of micrographic photographs are then given a score of 1-4.Result: Irrigated with 5% and 10% sodium thiosulfate showed no significant difference.Conclusion: The differences is not significant, 2,5% irrigation with sodium thiosulfate resulted in a higher cleanliness apical third of the root canalthan 2,5% NaOCl irigation with saline

THE EFFECT OF VARIOUS DESENSITIZING AGENT APPLICATION ON IN-OFFICE BLEACHING ON THE NUMBER OF FIBROBLASTS Nunik Rahayu Apriliyani; Ema Mulyawati; Yulita Kristanti
Odonto : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Reactive Oxygen Species (ROS) released from hydrogen peroxide causes inflammation of the pulp. Remineralization action by fluoride-containing desensitizing agent (Casein Phosphopeptides-Amorphous Calcium Fluoride Phosphate/CPP-ACFP) occluding dentinal tubule, thereby minimizing the penetration of hydrogen peroxide into the pulp and affect the number of fibroblasts. The aim of this study was to determine the effect of fluoride-containing desensitizing agent (CPP-ACFP) application: before, after, and before-after in-office bleaching techniques using 40% hydrogen peroxide on the number of fibroblasts.Method: Twenty-eight maxillary molar teeth were divided into 4 groups, Control Group (bleaching treatment), Group I (CPP-ACFP application before bleaching), Group II (CPP-ACFP application after bleaching), and Group III (CPP-ACFP application before and after bleaching). Rats were sacrificed 5 days after and histological preparations were stained with HE. Fibroblasts counting was performed using a 1000x magnification light microscope.Result: ANOVA test showed that there were significant mean differences in the number of fibroblasts among the groups. The LSD test showed that there were statistically significant differences in almost all of the compared groups except between the Control Group and Group II.Conclusion: The number of fibroblasts in the pulp of rats applied CPP-ACFP before and after in-office bleaching was less than applied CPP-ACFP before or after in-office bleaching.

Retrieval of broken file and retreatment of previously treated mandibular left second molar Paramastri, Sukma; Fitriningtyas, Kurnia; Santosa, Pribadi; Mulyawati, Ema; Rinastiti, Margareta; Kristanti, Yulita
Majalah Kedokteran Gigi Indonesia Vol 9, No 3 (2023): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.83545

Broken files in the root canal system which are not removed may interfere with thorough cleaning of the root canals. As a result, they cannot be hermetically obturated. This imperfect cleaning can leave necrotic tissue which can potentially cause failure in root canal treatment. The purpose of this paper is to describe the management of retrieval of broken files and re-treating root canals in previously treated mandibular left second molars. The retrieval of the broken file used conventional techniques combined with a Satelec ultrasonic scaler to remove the files from the root canal. It was operated under an endodontic microscope, and after retreatment of the root canal, the tooth was restored with final restoration of endocrown. Retrieval of fractured files using conventional techniques combined with Satelec ultrasonic scaler and the root canal re-treatment followed by final endocrown restoration could restore all four tooth functions properly.

Effect of sodium thiosulfate irrigation on push-out bond strength of root canal obturation material Panjiswastiawan, Leonardo Radito; Mulyawati, Ema; Kristanti, Yulita
Majalah Kedokteran Gigi Indonesia Vol 10, No 1 (2024): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.85125

Sodium hypochlorite is used as irrigation solution during root canal preparation because of its antibacterial effect. However, it degrades dentinal collagen and generates oxygen free layer on dentin surfaces that might prevent penetration of sealer. Sodium thiosulfate is an antioxidant and has been reported to neutralize that side effect. The objective of this study was to determine the effect of concentration and application time of sodium thiosulfate irrigation on push-out bond strength of root canal obturation material. This study used extracted human mandibular premolars single root post extraction. The root canals were prepared using crown down technique up to file F3. The samples were divided into four groups. Groups IA and IB were irrigated with sodium thiosulfate 5% for 3 and 5 minutes, while Groups IIA and IIB were irrigated with sodium thiosulfate 10% for 3 and 5 minutes. The control group was irrigated with saline for 3 and 5 minutes. The root canals were obturated with epoxy resin sealer and gutta percha, and were incubated for 7 days. Samples were horizontally sectioned into 2 mm thickness in apical third and were tested with push-out technique using universal testing machine. The adherence failure of samples was observed using stereo microscope. Data were analyzed with two-way ANOVA. There was a significant effect between concentration of sodium thiosulfate on push-out bond strength (p < 0.05), but application time showed the same effect (p > 0.05). The application of antioxidant resulted in an increase in the push-out bond strength of root canal obturation material, with 10% sodium thiosulfate demonstrating the highest push-out bond strength.

Perawatan ulang saluran akar non-bedah dan restorasi ulang menggunakan pasak fiber reinforced composite Fajrianti, Henytaria; Ratih, Diatri Nari; Kristanti, Yulita
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 9, No 2 (2023)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.77445

Perawatan saluran akar (PSA) bertujuan untuk menghilangkan mikroorganisme dari saluran akar. Kegagalan PSA dapat disebabkan oleh faktor-faktor seperti obturasi yang tidak hermetis, underfilling, overfilling, dan kegagalan restorasi pasca-PSA. Penambahan retensi berupa pasak dan mahkota jaket resin komposit dapat menjadi pilihan pasca endodontik. Artikel ini bertujuan untuk melaporkan kasus perawatan saluran akar ulang (retreatment) diikuti dengan pasak fiber prefabricated dan restorasi mahkota resin komposit. Seorang wanita berusia 21 tahundirujuk ke klinik konservasi gigi dengan keluhan utama gigi depan atas nyeri berulang selama 2 minggu terakhir. Pasien pernah jatuh 4 tahun lalu dan sudah dilakukan perawatan saluran akar serta diberi mahkota akrilik. Terlihat adanya area yang terbuka pada daerah servikal labial mahkota tersebut disertai inflamasi gingiva disekitarnya. Pemeriksaan palpasi (-) dan perkusi (+). Tindakan perawatan berupa retreatment, pemasangan pasak fiber dan restorasi mahkota komposit memberikan hasil yang baik. Kasus perawatan ulang saluran akar memerlukan tahapan yang tepat, dengan penambahan pasak fiber dapat meningkatkan retensi restorasi pada gigi dengankehilangan struktur yang signifikan.

EFFECT OF EXTRACT GEL GREEN TEA (Camelia Sinensis) ON NEUTROPHIL OF POST EXTRACORONAL BLEACHING RAT’S TEETH Kristanti, Yulita; Handajani, Juni; Untara, Tri Endra; Santosa, Pribadi
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.212-220

Background: Free radicals produced by hydrogen peroxide after teeth bleaching which diffuse through the enamel and dentinal tubules can trigger inflammation of the pulp. To overcome this negative side effect, exogenous antioxidants have been proposed. This research was conducted to determine the effect of natural antioxidants, namely 5% and 10% green tea, which was applied on rat molars teeth that were bleached using 40% hydrogen peroxide. Methods: Fifteen molar teeth of male Wistar rats were bleached using 40% hydrogen peroxide on their occlusal surface. Furthermore, the control group (Group I) rinsed using warm distilled water, while in Group II and Group III, the teeth were rinsed using 5% and 10% green tea extract gel. Wistar rats were sacrificed on the fifth day after treatment, followed by hematoxylin-eosin staining. Histological examinations were observed under a light microscope using 400x magnification and the number of the neutrophil were counted. Result: One-way ANOVA test showed that there was a significant effect of green tea extract gel concentration on the number of neutrophils (p 0.05). Post hoc test results showed that there were significant differences in all pairs of groups compared (p 0.05). Conclusion: Group III which was treated using10% green tea extract gel has the lowest number of neutrophil compared to Group II which was treated using 5% green tea extract hel and the control group.

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