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Treatment of PLGA Nanoparticles Ointment-Ethanol Extract of Archidendron pauciflorum in the Wound Healing in Diabetic Mice Desak Made Malini; Yasmi Purnamasari Kuntana; Madihah Madihah; Wildanul Furqon; Wawan Hermawan
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.9256

Diabetic wounds lead to severe tissue damage and are diffi-cult to cure. Jengkol (Archidendron pauciflorum) is a plant commonly used by local Indonesian communities to treat diabetic wounds. The efficiency of herbal medicine still has a deficiency of its ability to reach the target organs, therefore nanotechnology is applied in the hope that all drug concentrations can reach the target organs successfully. This study aimed to evaluate the effectiveness of PLGA nanoparticle ointment-ethanol extract jengkol fruit peel (EEJFP) to accelerate the wound healing process in the skin of streptozotocin-induced diabetic mice. The research method used was experimental with a completely randomized design using six treatments and four replications. Diabe-tes was induced by intraperitoneal injection of streptozotocin 180 mg/ kg BW. Mice with a blood glucose level of ≥150 mg/dL were used for diabetic mice models. The incision wound created at the dorsolateral region of shaven skin at ±1 cm2 using sterile scissors. The treatments given were vaseline for Control Negative (CN) and Control Positive (CP), Betadine ointment (PB), 10% EEJFP ointment (P1), 5% PLGA nanoparticle ointment-EEJFP (P2), and 2.5% PLGA nanoparticle ointment-EEJFP (P3). The results showed that the administration of PLGA nanoparticles ointment-EEJFP with a concentration of 5% PLGA nanoparticle ointment-EEJFP (P2) resulted in the shortest wound length on day 3, 7 and 14; narrower granulation tissue; a larger number of blood capillaries; and denser collagen fibers (α <0.05) compared to CP and PB treatments. The administration of PLGA nanoparticle ointment-EEJFP with a concentration of 5% was the most effective concentration in accelerating wound healing in the skin of diabetic mice.

EFEK ANTIFIDAN ANDROGRAFOLIDA TERHADAP HISTOKIMIA ENZIM PENCERNAAN LARVA Plutella xylostella Wawan Hermawan; Hikmat Kasmara; Yasmi P. Kuntana
BIOTIKA Jurnal Ilmiah Biologi Vol 3, No 1 (2004): Biotika Juni 2004
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v3i1.259

Potensi Temulawak dan Yoghurt dalam Mencegah Hemolisis Serta Perubahan Kadar Hdl-Ldl pada Darah Mencit (Mus Musculus L) Korespondensi: yang diberi Minyak Jelantah Walida Tanzania; Kartiawati Alipin Alipin; Yasmi P. Kuntana
BIOTIKA Jurnal Ilmiah Biologi Vol 11, No 2 (2013): BIOTIKA DESEMBER 2013
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v11i2.10059

RESPON PEMBERIAN PHYTOESTROGEN BERASAL DARI TEPUNG KEDELAI PADA KELINCI (Oryctolagus cuniculus) LUAS JARINGAN INTERSTITIAL, SPERMATOGENESIS DAN KUALITAS SPERMA Yasmi Purnamasari Kuntana, -; Yetty Yusri Gani -; Kartiawati Alipin -
Bionatura Vol 11, No 1 (2009): Bionatura Maret 2009
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.31 KB)

Kedelai, sebagai salah satu bahan penyusun ransum ternak diketahui mengandung senyawa phytoestrogen. Akumulasi senyawa phytoestrogen ini dalam ternak jantan telah mempengaruhi sistem reproduksi mencakup perubahan anatomi makro, mikro, dan fungsi organ reproduksi, menghambat pertumbuhan sel gamet, kemampuan fertilisasi dan tingkah laku seksual. Penelitian ini dilakukan untuk mengetahui sejauh mana pengaruh pemberian tepung kedelai dan mencari dosis tepung kedelai yang tidak mengganggu terhadap luas jaringan interstitial, spermatogenesis dan kualitas sperma pada kelinci. Penelitian dilaksanakan secara eksperimental di laboratorium dengan menggunakan pola rancangan acak lengkap (RAL). Perlakuan terdiri dari 4 macam dosis tepung kedelai yaitu kontrol (K1), tepung kedelai dosis 123 mg/kg berat badan (bb) kelinci (K2), tepung kedelai dosis 246 mg/kg bb kelinci (K3) dan tepung kedelai dosis 490 mg/kg bb kelinci (K4). Setiap perlakuan diulang 4 kali. Kelinci jantan umur dua bulan digunakan sebagai hewan model berjumlah 16 ekor. Pengujian variabel meliputi pengukuran persentase sperma hidup, abnormalitas sperma, pengamatan spermatogenesis serta luas jaringan interstitial. Data hasil pengujian variabel dianalisis menggunakan Analisis Varians (ANAVA) dan Uji Duncan. Hasil penelitian menunjukkan bahwa pemberian tepung kedelai sebagai sumber phytoestrogen pada kelinci menurunkan luas jaringan interstitial, spermatogenesis dan kualitas sperma akan tetapi dari ketiga dosis tepung kedelai yang diberikan, dosis 123 mg/kg bb kelinci memberikan hasil yang sama dengan kontrol bagi terbentuknya sperma hidup, terbentuknya abnormalitas sperma dan luas jaringan interstitial sehingga dapat dikatakan dosis 123 mg/kg bb kelinci adalah dosis yang relatif aman diberikan pada kelinci.Kata Kunci : phytoestrogen, tepung kedelai, kualitas sperma, spermatogenesis, luas jaringan interstitial, kelinci.

KARAKTERISTIK COLLUMNA VERTEBRALIS DAN COSTAE MUSANG LUWAK (Paradoxurus hermaphroditus) Yasmi Purnamasari Kuntana; Andi Hiroyuki; Satria Ardi Tama
BIOTIKA Jurnal Ilmiah Biologi Vol 20, No 1 (2022): BIOTIKA JUNI 2022
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v20i1.38814

Peraturan Menteri Pertanian Republik Indonesia Nomor37/Permentan/KB.120/6/2015 tentang cara produksi kopi Luwak harus melalui pemeliharaan Luwak yang baik yaitu dengan mengikuti prinsip kesejahteraan hewan sehingga, hewan dapat dengan bebas mengekspresikan perilaku alamiahnya. Perilaku alamiah musang luwak dapat dilihat dengan cara mengamati bentuk anatomi skelet. Penelitian ini bertujuan untuk mengetahui karakteristik collumna vertebralis dan costae musang luwak yang dikaitkan dengan perilaku alamiahnya. Penelitian dilakukan di bawah pengawasan dan persetujuan Komisi Etik Penelitian UNPAD (No. 548/UN6.KEP/EC/2020). Subjek penelitian menggunakan musang luwak (P. hermaphroditus) jantan umur 3 tahun dengan bobot badan 3,6 kg berjumlah satu ekor yang didapatkan dari penangkaran yang telah tersertifikasi. Penelitian menggunakan metode kualitatif dengan pendekatan deskriptif eksploratif. Parameter pengamatan meliputi anatomi skelet sumbu tubuh musang luwak. Pengumpulan data dilakukan dengan mengamati bentuk dan ukuran. Data yang telah didapatkan dijelaskan secara deskriptif yang mengacu pada literatur anatomi anjing dan kucing. Penulisan data dilakukan dengan mengacu pada Nomina Anatomica Veterinaria. Hasil pengamatan menunjukan bahwa sumbu tubuh musang luwak, yaitu memiliki foramen alare pada os atlas, mempunyai stout triangular pada processus spinosus os axis, terdapat os vertebrae diaphragmatica, dan memiliki panjang ekor melebihi panjang tubuhnya yang merupakan ciri hewan arboreal. Karakteristik skelet sumbu tubuh musang luwak merupakan adaptasi terhadap perilaku musang luwak yang hidup secara arboreal dan suka berpindah tempat.

Noni simplistic effect with Chicken Shank Gelatin Film on White Rat Spleen Exposed to Dexamethasone Virita Rossa Pratiwi; Yasmi Purnamasari Kuntana; Ruly Budiono; Desak Made Malini; Joko Kusmoro
Jurnal Veteriner Vol 24 No 1 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.1.122

Dexamethasone is a corticosteroid drug belong to glucocorticoid group. Dexamethasone is immunosuppressant and anti-inflammatory in various inflammatory conditions. Side effects of its use can cause cell apoptosis in various organs such as the spleen. The immunosuppressant effect of dexamethasone can reduce and inhibit peripheral lymphocytes and macrophages until the death of lymphoid cells in the white pulps of the spleen. The simultaneous effect of administering chicken shank gelatin and noni has the potential to improve the structure of the spleen. This study was aimed to prove and obtain effective and safe dose of chicken shank gelatin and noni on the spleens of rats exposed to dexamethasone. The research was carried out experimentally in the laboratory with a completely randomized design (CRD). A total of 25 heads male rats were grouped into five treatments and each treatment consist of five repetitions. There was a treatment group P1 as a negative control, P2 as a positive control (dexamethasone 5 mg/kg BW), P3-P5 (dexamethasone 5 mg/kg + gelatin 1.585 mg/kg + noni simplicia 50; 112; 250 mg/kg BW). The results of the study showed an increase in the area of white pulps and a decrease in the percentage of necrotic cells in the spleen, however, it did not increase the relative weight of the spleen and serum albumin levels (P>0.05). In conclusion, the effective and safe dose for the spleen organs of rats exposed to dexamethasone is 250 mg/kg BW.

Histological Structure of Male Wistar Rats’ Stomach Fed with Yam Tuber Flour Supplementation Permana, Andre Dian; Kuntana, Yasmi Purnamasari; Malini, Desak Made
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2023): October 2023
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i3.7166

A residential rat might harm human health because it acts as a disease reservoir. It has been many efforts to control this rate using synthetic rodenticide. Nevertheless, synthetic rodenticides broke the environment and made rats resistant. Yam (Dioscorea hispida) tube application on rat’s bite could solve the problem. This study evaluated the histology of male Wistar rats (Rattus norvegicus) fed with bite block supplemented with different yam tuber flour concertation to control residential rat populations. Five different treatments were applied with five replications. The treatments were negative control and brodifacoum 0.005% (positive control), 30%, 50%, and 70% of yam tuber flour. The results show that yam tuber supplementation caused damage to male Wistar rat stomachs, as indicated by mucosal erosion and the presence of inflammatory cells. The statistical test indicated that stomach damage significantly differed among treatments, with the severest damages caused by 50% yam tuber supplementation. It could be concluded that the rat’s bite containing yam tuber flour caused stomach damage, and the feed bite containing 70% yam tuber flour was the most effective. This result proved that yam tuber has good potential as a natural rodenticide to control residential rat populations.

Histological Structure of Male Wistar Rats’ Stomach Fed with Yam Tuber Flour Supplementation Permana, Andre Dian; Kuntana, Yasmi Purnamasari; Malini, Desak Made
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2023): October 2023
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i3.7166

A residential rat might harm human health because it acts as a disease reservoir. It has been many efforts to control this rate using synthetic rodenticide. Nevertheless, synthetic rodenticides broke the environment and made rats resistant. Yam (Dioscorea hispida) tube application on rat’s bite could solve the problem. This study evaluated the histology of male Wistar rats (Rattus norvegicus) fed with bite block supplemented with different yam tuber flour concertation to control residential rat populations. Five different treatments were applied with five replications. The treatments were negative control and brodifacoum 0.005% (positive control), 30%, 50%, and 70% of yam tuber flour. The results show that yam tuber supplementation caused damage to male Wistar rat stomachs, as indicated by mucosal erosion and the presence of inflammatory cells. The statistical test indicated that stomach damage significantly differed among treatments, with the severest damages caused by 50% yam tuber supplementation. It could be concluded that the rat’s bite containing yam tuber flour caused stomach damage, and the feed bite containing 70% yam tuber flour was the most effective. This result proved that yam tuber has good potential as a natural rodenticide to control residential rat populations.

Gambaran Morfologis Ginjal Ayam yang Diberi Ransum Mengandung Temulawak Serta Pengaruhnya terhadap Bobot Badan Alipin, Kartiawati; Fadilah, Ardi Mufarriz; Kuntana, Yasmi Purnamasari
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2016: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (722.537 KB)

Temulawak merupakan tanaman obat asli Indonesia yang mempunyai khasiat sebagai antibakteri yang dapat digunakan sebagi antibiotik alami dalam mencegah maupun mengobati penyakit. Pemanfaatan temulawak tidak hanya untuk manusia namun dapat digunakan sebagai pencegah penyakit pada ayam broiler, hal ini dilakukan dengan tujuan sebagai alternatif pengganti penggunaan antibiotik selama pemeliharaan ayam. Tujuan penelitian ini dilakukan untuk mengetahui pengaruh kurkuminoid temulawak (Curcuma xanthorrhiza Roxb.) terhadap organ ginjal ayam broiler dilihat dari struktur morfologis serta pengaruhnya terhadap bobot badan. Metode penelitian secara eksperimental dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan enam ulangan yaitu R0 (kontrol), R1 (37 mg kurkuminoid /kg ransum), R2 (74mg kurkuminoid/kg ransum) dan R3 (111 mg kurkuminoid/kg ransum). Perlakuan diberikan terhadap ayam broiler umur empat hari hingga lima minggu secara adlibitum. Hasil penelitian menunjukkan pemberian kurkuminoid temulawak pada semua dosis perlakuan berpengaruh terhadap struktur morfologis ditandai dengan adanya perubahan warna pada ginjal. Kesimpulan penelitian adalah walaupun pemberian kurkuminoid temulawak berpengaruh terhadap morfologis ginjal ayam namun masih dalam batas normal karena bobot badan ayam broiler mengalami peningkatan dibandingkan dengan kontrol.

Differential Regulation of Slc40a1, Fth1, and Hmox1 by Deferasirox in Splenic Iron Overload Wibowo, Annisa Maharani; Kuntana, Yasmi Purnamasari; Arrizqiani, Tanendri; Safitri, Ratu
Sciences of Pharmacy Volume 4 Issue 4
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphar0404386

Iron overload, often arising from repeated transfusions in thalassemia major, disrupts iron homeostasis and induces oxidative stress. Deferasirox is a widely used oral chelator, yet its effects on splenic iron-regulatory gene expression remain unclear. This study investigated the impact of deferasirox on ferritin heavy chain (Fth1), ferroportin (Slc40a1), and heme oxygenase-1 (Hmox1) expression in a rat model of splenic iron overload. Eighteen male Wistar rats were randomly assigned into three groups (n = 6 each): normal (N), iron dextran-induced overload without treatment (KN), and iron overload treated with deferasirox (KP). Gene expression was quantified by real-time PCR using the 2−ΔΔCT (Livak) method, with statistical analysis performed via one-way ANOVA and Tukey’s post hoc test. Iron overload significantly upregulated Fth1 (2.26-fold) and Slc40a1 (1.72-fold) versus controls (p < 0.05). Deferasirox treatment reduced Fth1 (3.28-fold decrease) and Slc40a1 (1.15-fold reduction) relative to untreated overload, though not significantly (p > 0.05). In contrast, Hmox1 expression markedly increased (55.25-fold, p < 0.05) following deferasirox administration. These results indicate that deferasirox selectively modulates splenic iron-regulatory genes, suggesting both chelation and adaptive stress-response mechanisms, thereby supporting its therapeutic role in managing iron overload.

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