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All Journal Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan FITOFARMAKA: Jurnal Ilmiah Farmasi Generics: Journal of Research in Pharmacy Proceeding ISETH (International Summit on Science, Technology, and Humanity) Borobudur Pharmacy Review Indonesian Journal of Pharmacy and Natural Product Jurnal Penelitian dan Kajian Ilmiah Kesehatan Politeknik Medica Farma Husada Mataram Generics : Journal of Research in Pharmacy
Melati Aprilliana Ramadhani, Melati Aprilliana
Universitas Ngudi Waluyo
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Skrining Fitokimia Dan Penetapan Kadar Flavonoid Total Serta Fenolik Total Ekstrak Daun Insulin (Tithonia diversifolia) Dengan Maserasi Menggunakan Pelarut Etanol 96 % Ramadhani, Melati Aprilliana; Hati, Anita Kumala; Lukitasari, Novel Fibriani; Jusman, Armin Hari
Indonesian Journal of Pharmacy and Natural Product Vol. 3 No. 1 (2020)
Publisher : Universitas Ngudi Waluyo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (520.64 KB) | DOI: 10.35473/ijpnp.v3i1.481

Abstract

LDaun insulin (Tithonia diversifolia) secara empiris digunakan oleh masyarakat Indonesia untuk menurunkan kadar gula darah. Senyawa aktif dalam ekstrak etanol daun insulin yang diprediksi memiliki aktivitas penurunan kadar gula darah adalah senyawa flavonoid dan fenolik. Ekstraksi dengan metode maserasi diharapkan dapat menjaga flavonoid dan fenolik dari kerusakan akibat pemanasan. Penelitian dilakukan untuk mengetahui metabolit sekunder dan kadar flavonoid total serta fenolik total dalam ekstrak etanol 96% daun Insulin (Tithonia diversifolia) yang diekstraksi dengan metode maserasi. Penelitian ini merupakan eksperimental laboratorium untuk skrining fitokimia dan menetapkan kadar flavonoid total serta fenolik total daun insulin (Tithonia diversifolia) dengan metode maserasi. Kadar flavonoid dan kadar fenolik ditetapkan menggunakan metode spektrofotometri visible. Flavonoid ditetapkan kadarnya berdasarkan pembentukan senyawa kompleks aluminium klorida, dengan standar baku kuersetin. Kadar fenolik ditetapkan berdasarkan pembentukan senyawa kompleks momolibdenum-tungsten, dengan standar baku asam galat. Hasil penelitian adalah skrining fitokimia ekstrak daun insulin menunjukkan bahwa daun insulin mengandung senyawa flavonoid, alkaloid, saponin, tanin dan fenolik. Kadar flavonoid dalam ekstrak etanol 96% daun insulin (Tithonia diversifolia) yang diperoleh metode ekstraksi maserasi adalah sebesar 90,58 mgEQ/gram ekstrak dan kadar fenolik sebesar 67,41 mgEAG/gram ekstrak. Kadar Flavonoid total dalam ekstrak etanol 96% daun Insulin lebih tinggi daripada kadar Fenolik total.Kata kunci : Tithonia diversifolia, Flavonoid, Fenolik, MaserasiInsulin leaf (Tithonia diversifolia) is empirically used by Indonesian people to reduce blood sugar levels. The active compounds in the ethanol extract of insulin leaves which are predicted to have decreased blood sugar levels are flavonoids and phenolic compounds. Extraction using maceration method is expected to be able to protect Flavonoids and Phenolics from heating damage. The study was conducted to find out the secondary metabolites, anda determine total flavonoid and total phenolic levels in ethanol 96% extract of Insulin (Tithonia diversifolia) leaves extracted by maceration method. This research is an experimental laboratory to phytochemical screening, and determine the levels of total flavonoids and total phenolic of insulin leaves (Tithonia diversifolia) by maceration method. Flavonoid levels and phenolic levels were determined using visible spectrophotometry methods. Flavonoid levels are determined based on the formation of aluminum chloride complex compounds, with the standard of quercetin. Phenolic levels are determined based on the formation of the compound momolybdenum-tungsten complex, with gallic acid standard. The result of this research are phytochemical screening of insulin leaf extracts shows that insulin leaves contain flavonoid, alkaloid, saponin, tannin, and phenolic. The results showed average concentration of flavonoids in the extract are 90,58 mgQE/ gram extract and  concentration of phenolic 67,41 mgGAE/ gram extract.nTotal flavonoid levels in ethanol extract 96% of Insulin leaves are higher than total phenolic levels.Keywords : Tithonia diversifolia, Flavonoid, Phenolic, Maceration 
Uji Kuantitatif flavonoid dan Aktivitas Antioksidan Teh Kombucha Daun Kersen (Muntingia calabura) Nintiasari, Jeinica; Ramadhani, Melati Aprilliana
Indonesian Journal of Pharmacy and Natural Product Vol. 5 No. 2 (2022)
Publisher : Universitas Ngudi Waluyo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.643 KB) | DOI: 10.35473/ijpnp.v5i2.1887

Abstract

Cherry leaf is a plant that contains high antioxidants to help protect the body from cell damage due to free radicals, steeping cherry leaves can be used as a health drink that contains more antioxidants, which is processed into kombucha tea. Kombucha is a fermented beverage made from a solution of tea and sugar by adding kombucha microbial starter and several types of yeast. This research is an experimental study. Determination of total flavonoid content and antioxidant activity test using UV-Vis spectrophotometry method. Flavonoids were determined based on the formation of aluminum chloride reagent complex compound with quercetin standard. Analysis of antioxidant activity test using the DPPH method with quercetin as a comparison. Phytochemical screening results showed that cherry leaf kombucha tea contains flavonoid compounds, tannins, saponins, alkaloids and phenols. The result of testing the total flavonoid content in kombucha tea preparations of cherry leaves was 44,026 mg/g quercetin. The results of the determination of antioxidant activity with the IC50 parameter in cherry leaf kombucha tea were 7.66 ppm with a very strong category.ABSTRAKDaun kersen merupakan tanaman yang mengandung antioksidan tinggi untuk membantu melindungi tubuh dari kerusakan sel akibat radikal bebas. Seduhan daun kersen dapat dimanfaatkan sebagai minuman kesehatan yang mengandung lebih banyak antioksidan yaitu diolah menjadi teh kombucha. Kombucha adalah minuman hasil fermentasi larutan teh dan gula dengan menambahkan starter mikrobia kombucha dan beberapa jenis khamir. Tujuan penelitian ini adalah untuk mengetahui kadar flavonoid total serta aktivitas antioksidan pada teh kombucha daun kersen. Penelitian ini merupakan penelitian eksperimental dengan analisis kadar flavonoid total serta uji aktivitas antioksidan menggunakan metode spektrofotometri UV-Vis. Flavonoid ditetapkan berdasarkan pembentukan senyawa kompleks reagen alumunium klorida dengan standar baku kuersetin. Analisa uji aktivitas antioksidan menggunakan metode DPPH dengan pembanding kuersetin. Hasil skrining fitokimia menunjukkan bahwa teh kombucha daun kersen mengandung senyawa flavonoid, tanin, saponin, alkaloid dan fenol. Hasil pengujian kadar flavonoid total pada sedian teh kombucha daun kersen adalah 44,026 mg/g kuersetin. Hasil penetapan aktivitas antioksidan dengan nilai %inhibisi pada konsentrasi 1 ppm didapatkan 28,28%, 2 ppm didapatkan 30,26%, 3 ppm didapatkan 33,80%, 4 ppm didapatkan 37,19%, 5 ppm didapatkan 41,86% dan nilai IC50 7,66 ppm dengan kategori sangat kuat.
Uji Aktivitas Antibakteri Berbagai Ekstrak Tanaman Herbal Terhadap Staphylococcus epidermidis Ramadhani, Melati Aprilliana; Nadifah, Silfia Duratun; Putri, Nurul Aulia; Sulastri, Sulastri
Generics: Journal of Research in Pharmacy Vol 4, No 1 (2024): Generics : Journal of Research in Pharmacy, Volume 4, Edisi 1, 2024
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/genres.v4i1.22681

Abstract

Acne vulgaris atau jerawat merupakan salah satu penyakit infeksi yang dapat menyerang kulit. Bakteri yang menjadi penyebabnya adalah Staphylococcus epidermidis. Beberapa bahan alam yang memiliki aktivitas sebagai antibakteri yaitu daun cengkeh (Syzygium aromaticum L), daun pepaya (Carica papaya L), dan daun melinjo (Gnetum gnemon L). Tujuan penelitian ini adalah menganalisis potensi antibakteri pada ekstrak daun cengkeh (EDC), papaya (EDP), dan melinjo (EDM) berdasarkan nilai zona hambat. Metode penelitian yang digunakan adalah eksperimental dengan analisis kualitatif metabolit sekunder dan pengujian aktivitas antibakteri menggunakan metode kertas cakram dengan konsentrasi ekstrak adalah 5%, 10%, 15%, dan 20%. Analisis hasil menggunakan SPSS versi 25. Hasil uji kualitatif masing-masing ekstrak mengandung flavonoid, alkaloid, saponin, dan tanin. Rerata zona hambat (mm) pada EDC, EDP, dan EDM pada konsentrasi 5% berturut-turut adalah 7,35; 1,33 ; 4,38; konsentrasi 10% adalah 8,25; 2,34; 5,60; kemudian pada konsentrasi 15% adalah 9,21; 3,54; 6,47; dan konsentrasi 20% adalah 10,28; 4,31; dan 7,59. Perbedaan nilai zona hambat dengan uji LSD antara EDC, EDP, dan EDM adalah < 0,05. Kesimpulan penelitian ini adalah aktivitas antibakteri EDC, EDP, dan EDM memiliki zona hambat terhadap bakteri Staphylococcus epidermidis yang berbeda signifikan. EDC memiliki aktivitas antibakteri yang paling baik.
Uji Aktivitas Antibakteri Mouthwash dan Antioksidan Minyak Atsiri Kulit Jeruk Nipis (Citrus aurantifolia): Antioxidant and Antibacterial Activity Test of Lime Essential Oil (Citrus aurantifolia) Ramadhani, Melati Aprilliana; Anhuma Turaya, M.Ridho; Aminah, Maulidahul; Mardiyanti, Devi; Rissa Laila Vifta; Sulastri
Indonesian Journal of Pharmacy and Natural Product Vol. 8 No. 01 (2025): Indonesian Journal of Pharmacy and Natural Product
Publisher : Universitas Ngudi Waluyo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35473/ijpnp.v8i01.4178

Abstract

Antibacterial and antioxidant activities are two frequently studied pharmacological properties of medicinal plants. Lime is a plant that possesses both of these activities is. One potential secondary metabolite is essential oil, which is abundantly found in the peel. The purpose of this study was to analyze the activity of lime peel essential oil (MAKJN) mouthwash against Streptococcus mutans and the antioxidant activity of MAKJN. The research methods used were phytochemical screening of MAKJN using color and GCMS tests, antibacterial testing of the mouthwash using the paper disc method with MAKJN concentrations of 1%, 2%, and 3%, and antioxidant testing using DPPH. Data were analyzed using SPSS version 27 with the LSD post-hoc test method. Phytochemical screening of MAKJN revealed flavonoids, alkaloids, terpenoids, and saponins, while GCMS results indicated the most abundant compound was l-Limonene. The antibacterial test of MAKJN mouthwash at concentrations of 1%, 2%, and 3% respectively had an average inhibition zone (mm) of 3.65; 3.97; and 5.82. SPSS analysis using the LSD test, namely the F3 inhibition zone had a p value <0.05 when compared with F1 and F2 which showed a significant difference. The antioxidant activity of MAKJN produced an IC50 value of 80.060 ppm. The conclusion of this study is that MAKJN mouthwash has potential as an antibacterial against Streptococcus mutans at a concentration of 3% with moderate potential, and MAKJN's antioxidant activity has strong potential.   ABSTRAK Antibakteri dan antioksidan adalah dua khasiat farmakologis pada tanaman obat yang sering diteliti. Jeruk nipis adalah tanaman yang memiliki kedua aktivitas tersebut. Salah satu metabolit sekunder yang berpotensi adalah minyak atsiri, yang banyak terkandung pada bagian kulitnya. Tujuan dari penelitian ini adalah menganalisis aktivitas mouthwash minyak atsiri kulit jeruk nipis (MAKJN) terhadap bakteri Streptococcus mutan dan antioksidan MAKJN. Metode penelitian yang digunakan adalah skrining fitokimia MAKJN dengan uji warna dan GCMS, uji antibakteri pada mouthwash menggunakan metode kertas cakram dengan konsentrasi MAKJN 1%, 2%, 3%, dan uji antioksidan menggunakan DPPH. Data dianalisis menggunakan SPSS versi 27 dengan metode uji post-hoc LSD. Skrining fitokimia pada MAKJN menunjukkan kandungan flavonoid, alkaloid, terpenoid, dan saponin, sedangkan hasil GCMS menunjukkan senyawa terbanyak adalah l-Limonene. Uji antibakteri mouthwash MAKJN pada konsentrasi 1%, 2%, 3% berturut-turut memiliki zona hambat rata-rata (mm) 3,65; 3,97; dan 5,82. Analisis SPSS menggunakan uji LSD menunjukkan zona hambat F3 memiliki nilai p<0,05 jika dibandingkan dengan F1 dan F2 yang menunjukkan perbedaan yang signifikan. Aktivitas antioksidan MAKJN ditunjukkan dengan nilai IC50 sebesar 80,060 ppm. Simpulan penelitian ini adalah mouthwash MAKJN memiliki potensi sebagai antibakteri terhadap Streptococcus mutan pada konsentrasi 3% dengan potensi sedang, dan aktivitas antioksidan MAKJN memiliki potensi yang kuat.
Genetic Characteristics of Actinomycetes Isolates (GST, KP, KP11, Kp16, T24, T37 Code) by RFLP of NRPS Genes Ramadhani, Melati Aprilliana; Sulistyani, Nanik
Media Farmasi: Jurnal Ilmu Farmasi Vol. 12 No. 1: Maret 2015
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/mf.v12i1.3020

Abstract

Actinomycetes is the one of antibiotic producing microorganisms. Actinomycetes isolates with GST, KP, KP11, KP16, T24, T37 have been isolated from soil. This study aims to determine the diversity of actinomycetes isolates by RFLP (Restriction Fragment Length Polymorphism) of NRPS (Non Ribosomal Peptide Synthetase) genes from GST, KP, KP11, KP16, T24, and T37 isolates. This study is divided into several steps of the DNA isolation  from isolate GST, KP16, T24, T37, KP, KP11, PCR of the 16S rRNA and NRPS genes and cutting the NRPS genes fragmens with HaeIII enzyme to determine the diversity of isolates. Results of DNA isolate, PCR of 16S rRNA genes and NRPS genes, and RFLP of NRPS genes performed by agarose gel electrophoresis. The diversity of the isolates were analyzed by multivariate analysis UPGMA with MVSP 2.0 applications. The results showed the diversity of 6 actinomycetes isolates are T37 and GST have 64 % similarity, KP11 and KP16 have 96 % similarity, KP has 76 % similarity with KP11 and KP16, while T24 has 60 % similarity with KP and 12 % similarity with T37 and GST. Based on RFLP analysis of the NRPS genes, among of 6 isolates can divided into 4 groups are T37 and GST isolates as group 1, KP11 and KP16 isolates as group 2, KP isolate as group 3, and T24 isolate as group 4.
In Vivo Antihypercholesterolemic Effects of Caricapubescens Seeds Hypercholesterolemic-induced Rats Luhurningtyas, Fania Putri; Aprilliana, Melati; Alifa, Gea Ros
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2019: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.1428

Abstract

Background: Carica is a typical plant originating from the Dieng Plateau. Carica processed products leave waste in the form of Carica seeds which have not been utilized. Based on the previous study, Carica seeds contain tannin, flavonoids, and alkaloids, so in this study, Carica seeds extract was used in the antihyperlipidemic study. Objective: To analyze the reduction of total cholesterol and secondary metabolites in the ethanol extract of Carica seeds using thin-layer chromatography.Method:Extraction was carried out by the maceration method using 96% ethanol. Antihypercholesterolemic study used 6 groups of rats Wistar strains consisting of normal group, positive control, negative control, treatment 1 (200 mg / KgBB), treatment 2 (400 mg / KgBB), treatment 3 (600 mg / KgBB). Total cholesterol level tests were carried out by using a biosystem with diasysic reagents. Statistical tests were carried out with one way ANOVA followed by LSD post hoc tests.Result: Carica seed extract has flavonoids and alkaloids. The percentage decrease in cholesterol levels in treatments 1, 2 and 3 was 15% ± 11,619, 24% ± 8,813 and 21% ± 19,471 with a significance value in the one way ANOVA test was 0,000. Conclusion: The dosage variations of Carica seed extract Affects comparable to simvastatin in reducing total cholesterol levels.
Upaya pencegahan dan pengobatan diabetes melitus di RPLSU Wening Wardoyo Kabupaten Semarang Ramadhani, Melati Aprilliana; Suyudi, Salsabiela Dwiyudrisa; Pradana, Andi; Untari, Untari; Maulana, Muhammad Rifqi; Hikmawati, Septiana Rohama Ria; Ramadhani, Afni Nadya
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 10, No 1 (2026): February (In Progress)
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v10i1.34125

Abstract

Abstrak Era modern saat ini dan struktur masyarakat yang juga berubah seiring dengan berjalannya waktu menyebabkan terjadinya masa transisi dari pola hidup masyarakat agraris menuju masyarakat industri. Adanya perubahan tersebut maka terjadilah perubahan pada pola makan dan aktivitas fisik di masyarakat, yang menyebabkan terjadi penyakit gangguan metabolisme, salah satunya Diabetes Mellitus (DM). Rumah Pelayanan Sosial Lanjut Usia (RPLSU) Wening Wardoyo merupakan mitra pada program PkM ini, dimana banyak lansia yang memiliki riwayat penyakit DM. Tujuan kegiatan PkM ini adalah meningkatkan pengetahuan tentang pencegahan dan pengobatan DM melalui usaha pembuatan jamu secara sederhana yang dapat digunakan untuk membantu mengatasi kadar gula darah yang tinggi, serta makanan dan minuman yang perlu diwaspadai. Metode pelaksanaan pengabdian ini adalah dilakukan dengan tatap muka secara langsung dengan sasaran bapak ibu lansia. Tahapan pelaksanaan kegiatan meliputi sosialisasi, persiapan, partisipasi mitra, pelaksanaan pretest, edukasi dan pelatihan, serta pendampingan dan evaluasi. Hasil pengabdian ini adalah terdapat kenaikan pengetahuan peserta, dengan % rata-rata peningkatan pengetahuan adalah 33,92%. Setiap pertanyaan terdapat peningkatan pengetahuan secara signifikan antara hasil pretest dan postest. Pertanyaan nomor 1 dan 2 memiliki persentase pengetahuan yang paling tinggi yaitu 91,67%. Hasil tersebut menunjukkan bahwa kegiatan pengabdian ini dapat meningkatkan pengetahuan peserta tentang cara-cara yang dapat digunakan untuk untuk mengontrol kadar gula. Kesimpulan pengabdian ini adalah kegiatan ini memberikan manfaat kepada peserta tentang diabetes melitus, pola makan yang perlu diwaspadai, dan pembuatan herbal secara sederhana terlihat dari peningkatan presentase pada kuisioner posttest. Kata kunci: diabetes mellitus; pengobatan herbal; lansia; peningkatan pengetahuan. Abstract The modern era and the changing structure of society over time have led to a transition from an agrarian lifestyle to an industrial one. This change has resulted in changes in dietary patterns and physical activity in society, leading to metabolic disorders, one of which is diabetes mellitus (DM). The Wening Wardoyo Elderly Social Service Centre (RPLSU) is a partner in this PkM programme, where many elderly people have a history of DM. The aim of this PkM activity is to increase knowledge about the prevention and treatment of DM through the simple preparation of herbal medicine that can be used to help control high blood sugar levels, as well as foods and drinks that need to be watched out for. The implementation method of this community service was conducted through direct face-to-face interactions with the elderly participants. The stages of the activity included socialisation, preparation, partner participation, pre-test implementation, education and training, as well as mentoring and evaluation. The results of this community service showed an increase in participants' knowledge, with an average knowledge improvement rate of 33.92%. Each question shows a significant increase in knowledge between the pre-test and post-test results. Questions 1 and 2 have the highest knowledge percentage, namely 91.67%. The results indicate that this community service activity increased participants' knowledge of methods for controlling blood sugar levels. The conclusion is that this community service activity provided participants with valuable information about diabetes mellitus, dietary concerns, and simple herbal preparations, as evidenced by the increased percentage of participants on the post-test questionnaire Keywords: diabetes mellitus; herbal medicine; elderly;, knowledge enhancement.
Co-Authors Alifa, Gea Ros Aminah, Maulidahul Anhuma Turaya, M.Ridho Armin Hari Jusman Armin Hari Jusman Ayu Prabandari Novema Deny Saputra Dian Oktianti Elisa Issusilaningtyas Hasani, Nova Hati, Anita Kumala Hikmawati, Septiana Rohama Ria Jusman, Armin Hari Luhurningtyas, Fania Putri Lukitasari, Novel Fibriani Mardiyanti, Devi Muhammad Rifqi Maulana Nadifah, Silfia Duratun Nanik Sulistyan Sulistyan Nanik Sulistyani Nintiasari, Jeinica Novel Fibriani L Novel Fibriani Lukitasari Nur Amin Pradana, Andi Pratiwi, Neli Diah Prayasanti, Dewi Putri, Nurul Aulia Ramadhani, Afni Nadya SULASTRI Sulastri Sulastri Sulistyan, Nanik Sulistyan Suyudi, Salsabiela Dwiyudrisa Untari Untari vifta, rissa laila