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Efektivitas Metode Multi Soil Layering (MSL) dalam Penurunan Total Koliform Limbah Cair Domestik Shakira, Aisha; Mullah, Ama; Hamdan, Abd Mujahid; Lubis, Syafrina Sari
Dampak Vol. 20 No. 2 (2023)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/dampak.20.2.83-92.2023

Abstract

This research employs Multi Soil Layering (MSL), an environmentally friendly technology, to remove coliform bacteria contaminants from wastewater using a novel alternative medium. The purpose of this study is to evaluate the efficacy of the MSL method employing bio rings in reducing pollutant parameters in domestic wastewater, particularly total coliform. This study utilized Hydraulic Loading Rate (HLR) variations of 23.80 L/m2/hour, 7.14 L/m2/hour, and 3.40 L/m2/hour, as well as Hydraulic Retention Time (HRT) variations of 4, 6, 8, 10, and 12 hours. The results of domestic wastewater treatment using the MSL method using HLR variations on total coliform from 1.89  105 MPN/100mL to 6.0  102 MPN/100mL, the total colony parameter from 5.95  106 CFU/mL to 3.6  105 CFU/mL, the chemical oxygen demand parameter value of 53 mg/L with an effectiveness of 98.6%, the total suspended solid parameter is valued at 25 mg/L with reduction effectiveness of 98.6% and the pH value changes to 7.3 at the HLR variation of 3.40 L/m2/hour. As for using the HRT variation for 12 hours the total coliform from 1.16  104 MPN/100mL to 9  102 MPN/100mL, the total colony parameter from 4.91  106 CFU/mL to 3.0  105 CFU/mL, the chemical oxygen demand parameter was 92 mg/L with its effectiveness 99%, the total suspended solid parameter is 87 mg/L with reduction effectiveness of 67% and the potential of hydrogen value changes to 7.6. Therefore, the MSL method employing bio rings can be used as a new option for domestic effluent treatment. Keywords: domestic wastewater, coliform total, Multi Soil Layering (MSL)  ABSTRAK Penelitian ini menggunakan Multi Soil Layering (MSL), sebuah teknologi ramah lingkungan, untuk menghilangkan kontaminan bakteri coliform dari air limbah menggunakan media alternatif baru. Tujuan dari penelitian ini adalah untuk mengevaluasi efikasi metode MSL dengan bioring dalam menurunkan parameter polutan dalam air limbah domestik, khususnya total coliform. Penelitian ini memanfaatkan variasi Hydraulic Loading Rate (HLR) 23,80 L/m2/jam, 7,14 L/m2/jam, dan 3,40 L/m2/jam, serta variasi Hydraulic Retention Time (HRT) 4, 6, 8, 10, dan 12 jam. Hasil pengolahan air limbah domestik dengan metode MSL menggunakan variasi HLR pada total coliform dari 1,89 × 105 MPN/100mL menjadi 6,0 × 102 MPN/100 mL, parameter total koloni dari 5,95 × 106 CFU/mL menjadi 3,6 × 105 CFU/mL, nilai parameter kebutuhan oksigen kimia 53 mg/L dengan efektivitas 98,6%, parameter total padatan tersuspensi senilai 25 mg/L dengan efektivitas reduksi 98,6% dan nilai pH berubah menjadi 7,3 pada variasi HLR 3,40 L/m2/jam. Sedangkan untuk menggunakan variasi HRT selama 12 jam total coliform dari 1,16 × 104 MPN/100mL menjadi 9 × 102 MPN/100mL, parameter total koloni dari 4,91 × 106 CFU/mL menjadi 3,0 × 105 CFU/mL, kebutuhan oksigen kimia parameter 92 mg/L dengan efektivitas 99%, parameter total padatan tersuspensi 87 mg/L dengan efektivitas reduksi 67% dan nilai potensial hidrogen berubah menjadi 7,6. Oleh karena itu, metode MSL yang menggunakan bio ring dapat digunakan sebagai pilihan baru untuk pengolahan limbah rumah tangga. Kata kunci: air limbah domestik, total koliform, Multi Soil Layering (MSL)      
INVESTIGASI PERILAKU OVIPOSISI Aedes aegypti PADA PERBEDAAN WARNA WADAH PENAMPUNGAN AIR DI KOTA MATARAM Munawir Sazali; Syafrina Sari Lubis
Jurnal Inovasi Pendidikan dan Sains Vol 5 No 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/jips.v5i1.2023

Abstract

Dengue fever was an endemic disease that transmission from Aedes sp. bites. The spread epidemic disease of dengue fever in an area was caused highest vector dengue virus population, it’s bolstered by water container as canister oviposition of Aedes aegypti at difference position (indoor and outdoor). Water container has been finding at residence area such as bathtub, well, disposal plastic, crockery, etc. This study aims to investigation oviposition process Ae. aegypti populations at Pagutan Sub-district in Mataram City. The research was explanatory in prevention community trial research. Based on data egg collection was known oviposition behavior of the mosquito, it was attracted to dark color water container at the indoor and outdoor position. The difference of color water container and position were the significant difference (p=0.000<0.05), it was found significant difference dark color of water container at an indoor position with bright and white color water container at an indoor position, and significant difference with dark, bright and white color at an outdoor position. Oviposition behavior of dengue vector viruses was developed as biological control of Aedes sp. based on color of water container.
Sekuensing Fungi Patogen Berbasis Oxford Nanophore Technology (ONT) pada Kulit Buah Kopi Arabika (Coffea arabica) dari Desa Tawar Miko, Aceh Tengah Fauziyah, Fauziyah; Lubis, Syafrina Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 4 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.4.18508

Abstract

Kopi arabika (Coffea arabica L.) di Aceh Tengah rentan terhadap penyakit busuk buah (coffee fruit rot/ CFR) yang disebabkan oleh kompleks patogen fungi. Penelitian ini bertujuan mengidentifikasi keanekaragaman fungi patogen pada kulit buah kopi arabika bergejala busuk hitam dari Desa Tawar Miko, Aceh Tengah, menggunakan metabarcoding berbasis Oxford Nanopore Technology. Sampel buah kopi yang busuk diambil secara acak, dilakukam ekstraksi DNA total, amplifikasi gen penanda Internal Transcribed Spacer (ITS) rDNA, dan sekuensing Oxford Nanopore. Data sekuens dianalisis secara bioinformatika untuk menentukan komposisi taksonomi dan menghitung indeks keanekaragaman. Hasil analisis mengidentifikasi 57 spesies dengan total 134.371 sekuens yang tergolong ke dalam 17 genus. Lima filum terdeteksi dengan dominasi Ascomycota (94,53%), diikuti oleh Unknown (5,40%), Zoopagomycota (0,04%), Bacillota (0,02%), dan Actinomycota (0,01%). Sepuluh genus utama menunjukkan kelimpahan yang bervariasi, yaitu Kurtzmaniella (48,64%), Penicillium (45,59%), Unknown (5,40%), Brettanomyces (0,07%), Chlamydocillium (0,07%), Scolecobasidium (0,06%), Brunneoclavispora (0,02%), Coemansia (0,02%), Pandora (0,02%), dan Other (0,11%). Tiga spesies dominan adalah Kurtzmaniella quercitrusa (48,60%), Penicillium crustosum (39,75%), dan Penicillium coprobium (3,79%). Nilai indeks Shannon (H′) sebesar 1,17 menunjukkan tingkat keanekaragaman yang rendah dengan dominansi tinggi (Simpson D = 0,6). Temuan ini memberikan gambaran dasar komunitas fungi patogen pada busuk buah kopi arabika dan berimplikasi sebagai dasar pengembangan strategi pengendalian penyakit tanaman untuk meningkatkan mutu dan keamanan kopi di Aceh Tengah. Kata kunci : busuk buah kopi,fungi patogen, sekuensing, Oxford Nanopore Technology, ITS rDNA
Analisis Metabarcoding ITS Plastifer dari Tempat Pembuangan Akhir Bukit Meusara Jantho Aceh Besar: Upaya Mitigasi Orangutan Sumatra (Pongo abelii) dari Risiko Cemaran Mikroplastik Napisyah, Pingkan; Lubis, Syafrina Sari
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18468

Abstract

This study aims to identify the diversity of plastic-colonizing fungi (plastisphere) at the Bukit Meusara Landfill in Jantho, Aceh Besar, located 5-8 km from the Sumatran Orangutan (Pongo abelii) rehabilitation center, using a metabarcoding approach with the Nanopore platform. Analysis results revealed 143 OTUs from 81,414 sequences with a Shannon index of 2.77, indicating high fungal diversity in the plastisphere. The fungal community was dominated by Ascomycota (83.2%), particularly the class Sordariomycetes (51.7%). Dominant species included Mycothermus thermophilus (9-10%), M. thermophiloides (6%), Brocchiosphaera bulbiformis (5%), and Thermomyces lanuginosus (4%). The identified thermophilic fungi are known to produce enzymes such as laccase, cutinase, lignin peroxidase, and manganese peroxidase, which have potential for degrading PET, PS plastics, and biodegradable polymers. This study provides baseline data on indigenous plastisphere diversity that can be developed as a bioremediation consortium for mitigating microplastic pollution in Sumatran orangutan conservation areas.
Rhizosphere Microbiome of Arabica Coffee (Coffea arabica L.) Analyzed Using Oxford Nanopore 16S Sequencing Nate, Selvia Pinte; Lubis, Syafrina Sari
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.10763

Abstract

The rhizosphere microbiome plays a key role in nutrient cycling, soil structure, and plant health in Coffea arabica. This study analyzed the bacterial community composition of Arabica coffee rhizosphere soil from Tawar Miko, Aceh Tengah, using full-length 16S rRNA sequencing on the Oxford Nanopore platform. Taxonomic profiling revealed ten dominant phyla, including Pseudomonadota (43.77%), Acidobacteriota (18.17%), Planctomycetota (11.23%), Actinomycetota (5.81%), Myxococcota (4.08%), Bacteroidota (3.35%), Bacillota (2.86%), Thermodesulfobacteriota (2.69%), and Nitrospirota (2.26%). At the genus level, the most abundant taxa were Vicinamibacter (8.61%), Bradyrhizobium (4.14%), Brevitalea (4.03%), Pseudolabrys (2.64%), Nitrospira (2.26%), Acidibacter (1.97%), Streptomyces (1.76%), Sandaracinobacter (1.70%), and Paraburkholderia (1.57%). Species-level assessment indicated that Bradyrhizobium sp. was the most abundant, followed by Vicinamibacter sp., Brevitalea sp., Nitrospira sp., Paraburkholderia sp., Pseudolabrys sp., and Acidibacter sp., while low-abundance species (<3%) included Streptomyces sp. and Sandaracinobacter sp. These dominant species contribute to nitrogen fixation, nitrite oxidation, organic matter degradation, hydrocarbon metabolism, and natural biocontrol activity. The balanced distribution across taxa indicates a stable and functionally complementary microbial community that supports soil health and enhances the growth of Arabica coffee in highland agroecosystems.
Ecoprint sebagai Upaya Peningkatan Literasi Pelajar di MAT Daarut Tahfidz Al-Ikhlas Banda Aceh Arif Sardi; Jamaluddinsyah Jamaluddinsyah; Raudhah Hayatillah; Syafrina Sari Lubis
Aksi Nyata : Jurnal Pengabdian Sosial dan Kemanusiaan Vol. 3 No. 1 (2026): Januari : Aksi Nyata : Jurnal Pengabdian Sosial dan Kemanusiaan
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/aksinyata.v3i1.2794

Abstract

This community service activity aims to enhance students’ literacy through the application of ecoprint as an environmentally based creative learning medium at MAT Daarut Tahfidz Al-Ikhlas Banda Aceh. This program was motivated by the limited utilization of practice-based art activities as a means of strengthening students’ literacy. The implementation method employed a participatory-educative approach consisting of material socialization, ecoprint technique demonstrations, group-based hands-on practice, reflective discussions, and activity evaluation. The results indicate that students were able to understand the concepts and stages of ecoprint, produce works with various natural motifs, and demonstrate improvements in visual literacy and creativity. In addition, the activity increased students’ awareness of the use of natural materials and the importance of environmental friendly practices. Group-based activities also encouraged the development of social literacy through collaboration and communication among participants. Therefore, ecoprint can be considered an effective and contextual alternative learning medium to support the enhancement of students’ literacy in the school environment and has the potential to be sustainably developed.
POTENSI DARK SEPTATE ENDOPHYTE (DSE) DALAM PERTUMBUHAN FASE VEGETATIF TANAMAN PADI (Oryza sativa L.) TERHADAP STRES KEKERINGAN Diani, Putri Laily Nurainum; Lubis, Syafrina Sari
BIO-SAINS : Jurnal Ilmiah Biologi Vol. 6 No. 1 (2026): Bio-Sains Vol. 6 No. 1, Maret 2026
Publisher : Fakultas Sains dan Teknologi, Universitas Islam As-Syafi'iyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34005/fjvpvr41

Abstract

Perubahan iklim menjadi tantangan yang menyebabkan penurunan produksi padi sekaligus meningkatnya kebutuhan beras, sehingga diperlukan upaya untuk meningkatkan toleransi tanaman terhadap cekaman kekeringan. Dark Septate Endophyte (DSE) merupakan jamur endofit akar yang bersimbiosis dengan tanaman inang, dicirikan oleh hifa bersekat dan pigmen gelap, serta berpotensi meningkatkan ketahanan tanaman terhadap stres abiotik. Penelitian ini bertujuan menganalisis potensi inokulasi DSE terhadap pertumbuhan vegetatif tanaman padi pada kondisi stres kekeringan. Penelitian menggunakan rancangan acak kelompok dengan empat perlakuan, yaitu P0 : Non-DSE + air optimal, P1 : DSE + air optimal, P2 : Non-DSE + kekeringan, dan P3 : DSE + kekeringan. Parameter yang diamati meliputi morfologi tanaman dan parameter fisiologis berupa kerapatan stomata serta pengamatan kolonisasi akar secara mikroskopis. Data morfologi dianalisis menggunakan Two-Way ANOVA, sedangkan parameter fisiologis dianalisis secara deskriptif. Hasil menunjukkan bahwa inokulasi DSE tidak memberikan pengaruh signifikan terhadap seluruh parameter morfologi tanaman padi. Namun, secara fisiologis tanaman yang diberi DSE menunjukkan kerapatan stomata lebih tinggi, aktivitas stomata lebih terbuka, serta keberhasilan kolonisasi akar yang ditandai oleh melanisasi pada jaringan epidermis. Temuan ini mengindikasikan bahwa DSE berpotensi mendukung respons fisiologis padi terhadap cekaman kekeringan.