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AKTIVITAS KITOSAN LIMBAH CANGKANG KERANG BULU (Anadara antiquata) TERHADAP Aeromonas hydrophila PADA IKAN NILA (Oreochromis niloticus) Tuti Aulia; Syafrina Sari Lubis; Diannita Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 2 No. 2 (2022): Oktober 2022
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v2i2.3033

Abstract

Aquaculture in Indonesia is carried out in freshwater, brackish and marine waters with limited production of several fish species such as tilapia. Although tilapia is easy to adapt to the environment, it can be infected with Aeromonas hydrophila bacteria. One of the efforts to treat bacteria is to use chitosan which is a chitin derivative that can be developed as an antimicrobial candidate because it contains lysozyme enzymes and aminopolysaccharide groups that can inhibit microbial growth. The purpose of this study was to determine the activity of chitosan from feather shell waste (Anadara antiquata) in inhibiting Aeromonas hydrophila in vitro and in vivo in tilapia. The methods used for the manufacture of chitosan include deproteination, demineralization, depigmentation, and deacetylation. In vitro testing was conducted to test the antibacterial activity using the disc diffusion method (Kirby-Bauer test). In vivo testing was carried out by the immersion method. The results showed that the chitosan yield was 77.2%. The most effective concentration of chitosan antibacterial activity against Aeromonas hydrophila was at 7% with an inhibition zone value of 6.85 mm. The results of data analysis showed that there was a significant difference between the test treatments. The in vivo test on tilapia showed that the highest percentage was found in the optimal concentration treatment (7% chitosan) with a value of 68.74%. The results of data analysis showed that there was no significant difference between optimal concentration treatment and positive control.
POTENSI ENZIM SELULASE DARI BAKTERI TERMOFILIK DI KAWASAN WISATA IE SUUM KABUPATEN ACEH BESAR Rahmatil Majidah; Syafrina Sari Lubis; Diannita Harahap
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 3 No. 1 (2023): April 2023
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v3i1.3119

Abstract

Thermophilic bacteria are a group of bacteria that grow at a temperature range of 45oC to 80oC and have the potential to produce cellulase enzymes. The tourist area of Ie Suum, Aceh Besar District is one of the hot springs in Aceh. This study aims to observe the morphological characteristics, cellulase enzyme potential and activity test in producing cellulase enzymes from thermophilic bacteria found in Ie Suum. The method of isolating thermophilic bacteria uses the pour plate method and testing the cellulase enzyme activity using the DNS (3,5-dinitrosalicylic Acid) method. 15 isolates were obtained, namely 13 Gram positive and 2 Gram negative, 2 genera were obtained, namely the genus Bacillus sp. and the genus Pseudomonas sp. Based on the potential of the cellulase enzyme, the 15 isolates showed the largest clear zone diameter, namely isolate TS6 3,67 cm, isolate TS7 2,33 cm, and isolate TS10 4,00 cm. The results of measuring enzyme activity using the DNS method obtained isolates TS6 4,7 x 10-3 U/mL, isolates TS7 4,4 x 10-3 U/mL, and isolates TS10 5,5 x 10-3 U/mL.
KARAKTERISASI DAN UJI POTENSI JAMUR ENDOFIT PADA DAUN BAWANG MERAH (Allium ascalonicum L.) SEBAGAI PENGENDALI PATOGEN Fusarium sp. dan Alternaria sp. Mita Erliza; Syafrina Sari Lubis
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 3 No. 1 (2023): April 2023
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v3i1.3120

Abstract

Shallot (Allium ascalonicum L.) is a horticultural commodity that has high economic value and is needed every day by the community. Attack by pets and pathogens on shallot cultivation cause a decrease in crop productivity. The pathogen that often attacks shallot plants is Fusariumsp. and Alternaria sp. This study aims to obtain endophytic fungi from shallot leaves that can control Fusarium sp. and Alternaria sp. The endophytic fungus isolation method uses healthy shallot leek samples while the pathogen isolation uses unhealthy leaves. The antagonism potency test was conducted using the dual culture method with an incubation time of 7 days at 25-30oC. Based on the results of the study, there were 4 isolates of endophytic fungi on shallot leaves, namely EA1 isolates (Aspergillus niger), EA2 isolates, EA3 isolates (Clasdoporium sp.) and EA4 isolates (Phyllosticta sp.). The results of the antagonist test showed that the endophytic fungus EA1 was able to inhibit the fungus Fusarium sp. and Alternaria sp. maximally (strong) with an average value of Fusarium sp. of 62.72% while Alternaria sp. 67.83 %. The endophytic fungus EA2 was also able to inhibit both of these pathogens but it was moderately categorized with an average value of Fusarium sp. 52.11 % and Alternaria sp. 53.28%. The mechanisms of inhibition are competition and hyperparasitism.
BIODIVERSITAS MIKROFUNGI PADA EKOSISTEM MANGROVE DI DESA GAMPONG PANDE KECAMATAN KUTA RAJA KOTA BANDA ACEH Desi Anggraini; Syafrina Sari Lubis
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 3 No. 2 (2023): Oktober 2023
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v3i2.4209

Abstract

Desa Gampong Pande merupakan kawasan ekosistem mangrove yang terletak di Desa Gampong Pande Kecamatan Kuta Raja Kota Banda Aceh. Keberadaaan pemukiman warga dan tambak di daerah tersebut mempercepat degradasi kawasan mangrove. Keberadaan mikrofungi pada ekosistem mangrove dapat menjadi bioindikator lingkungan. Penelitian ini bertujuan untuk mengetahui apa saja mikrofungi yang ditemukan serta untuk mengetahui nilai indeks keanekaragaman mikrofungi pada ekosistem mangrove Desa Gampong Pande Kecamatan Kuta Raja Kota Banda Aceh. Metode purposive sampling dan eksploratif digunakan untuk mendapatkan data keanekaragaman mikrofungi pada kawasan ini. Isolasi mikrofungi menggunakan metode cawan sebar. Pada stasiun 1 diperoleh total koloni mikrofungi 3,7 x 106 cfu/ml, stasiun 2 sebanyak koloni 3,2 x 106 cfu/ml dan stasiun 3 sebanyak 6,1 x 106 cfu/ml koloni. Sebaran mikrofungi terdiri dari genus Simplicillium, Aspergillus, Cunninghamella, Macrophomina, dan Mucor dengan nilai indeks keanekaragaman di stasiun 1 H’= 0,68, stasiun 2 dengan nilai H’= 0,29, dan stasiun 3 sebesar H’= 0,81 yang tergolong rendah karena nilai H’<1. Jika nilai H’<1 maka dapat diketahui bahwa keadaan suatu lingkungan tersebut memiliki keanekaragaman spesies yang rendah, kestabilan komunitas rendah dan keadaan lingkungan tercemar berat.
TOLERANSI TEMBAGA (Cu) PADA MIKROFUNGI ASPERGILLUS SP. YANG DIISOLASI DARI SEDIMEN SUNGAI KRUENG ACEH Munandar, Aris; Lubis, Syafrina Sari; Hamdan, Abd Mujahid
AMINA Vol. 5 No. 3 (2023): December 2023
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/amina.v5i3.4155

Abstract

Tembaga (Cu) memiliki sifat toksik bagi lingkungan apabila melebihi baku mutu standar yaitu 0,02 mg/L. Bagi beberapa mikroba tembaga (Cu) merupakan logam esensial yang berguna untuk pertumbuhan mikroorganisme. Keberadaan mikroba seperti mikrofungi dapat digunakan dalam bioremediasi logam Cu dilingngkungan. Penelitian ini bertujuan untuk mengetahui karakteristik mikrofungi Aspergillus dan kemampuan toleransi terhadap tembaga (Cu). Metode yang digunakan dalam penelitian ini adalah eksperimental.Hasil karakteristik morfologi menemukan isolat FS1 Aspergillus niger, isolat FS2 dan FS3 merupakan Aspergillus sp. Hasil uji toleransi terhadap Cu menunjukkan kemampuan toleransi paling tinggi yaitu pada isolat FS2 (120) 200 ppm, sedangkan kemampuan toleransi paling rendah yaitu pada isolat FS3 (24) 100 ppm.
CHARACTERISTICS AND CONCENTRATION OF AIRBORNE FUNGI IN PUBLIC BUS TRANSPORTATION IN BANDA ACEH CITY, ACEH PROVINCE Lubis, Syafrina Sari; Mehram Maina
Indonesian Journal of Environmental Sustainability Vol. 3 No. 1 (2025): June 2025
Publisher : Center for Environmental Studies, Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ijes.v3i1.7688

Abstract

Airborne fungi are one of the common pollutants that spread through the air and frequently found in public bus transportation. Their transmission of airborne fungi can have an impact on health and is considered an important public health problem. The presence of airborne fungi can cause various respiratory diseases such as asthma, bronchopulmonary infections, rhinitis and other allergic symptoms. The concentration of airborne fungi in buses has a significant correlation between the level of fungi and the number of passengers. High concentrations of fungi in transportation can affect human health. The purpose of this study was to determine the concentration of airborne fungi in buses and the types of fungi in the air on buses that had just arrived and those that were about to depart for Medan. The sampling method used for collecting airborne fungi is the probability sampling technique, especially by using the random sampling technique and the open plate technique which is left open for 15 minutes while measuring physical factors such as temperature, humidity, light intensity and number of passengers. Samples were taken from 3 buses X, Y and Z found on public transportation buses. The results of this study obtained the concentration of fungi in the air on buses that had just arrived and those that were leaving for Medan reaching 105, 9 CFU/m3 on bus X, 70, 6 CFU/m3 and 141, 2 CFU/m3 on bus Y, and 46, 9 CFU/m3 and 151, 8 CFU/m3 on bus Z, meaning that these values ​​do not exceed the indoor air quality standards set by WHO of < 500 CFU/m3. The types of airborne fungi on buses X, Y and Z are Alternaria sp., Aspergillus sp., A. niger, A. flavus, A. fumigatus, Penicillium sp., Clasdoporium sp., C. clasporioides, dan Acremonium sp.
Description of Tinea Unguium Infection Levels in Farmers' Nails in Meunasah Pupu Village, Ulim District, Pidie Jaya Regency Lubis, Syafrina Sari; Nacia, A’Arifa
International Journal of Science, Technology and Applications Vol 3 No 1 (2025): Volume 3 Number 1 Tahun 2025
Publisher : Alpatih Harapan Semesta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70115/ijsta.v3i1.270

Abstract

Tinea unguium infection is a fungal infection that attacks the nails characterized by cracks and the color of the nails changing to reddish brown, and yellowish. Tinea unguium occurs with a general condition that begins with spots or yellow on the toenails. Severe fungal infections can cause nails to blacken, thicken, and crumble at the edges. This infection can affect several nails but usually not all nails are infected. If the fungal infection on the nails is still relatively mild, it does not require treatment. Fungal nail infections can cause pain and thickening of the nails so that they require care and treatment. This study aims to identify the type of fungus that causes Tinea unguium in the nails of farmers in Meunasah Pupu Village, Ulim District, Pidie Jaya Regency. The method of taking samples of farmers' nails was carried out descriptively by conducting direct interviews. The sample involved 20 respondents who had nail damage. Nail scraping samples were grown on PDA media and incubated for 3-7 days at a temperature of 25 ⁰C. Based on the characterization, 23 isolates were obtained, namely Trycophyton rubrum (SB1), Trycophyton mentagrophyts (SC1), Epidermophyton floccosum (SG1, SH1, ST1), and Aspergillus sp.
POTENSI EKSTRAK DAUN ASAM KERANJI (Dialium indum) ACEH SEBAGAI ANTIOKSIDAN ALAMI Lubis, Syafrina Sari; Sari, Ayu Nirmala; Fahmi, M. Haikal; Diningrat, Diky Setya
Biofaal Journal Vol 2 No 1 (2021): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.364 KB) | DOI: 10.30598/biofaal.v2i1pp11-18

Abstract

Penelitian ini dilakukan untuk menguji aktivitas fitokimia ekstrak etanol asam keranji (Dialium indum) sebagai antioksidan secara in vitro. Aktivitas antioksidan yang dilakukan dalam beberapa model pengujian antioksidan secara in vitro yaitu menguji aktivitas reduksi radikal bebas DPPH, radikal superoksida, daya pereduksi ferric dan aktivitas hidrogen peroksida. Kapasitas total antioksidan juga ditentukan. Ekstrak etanol asam keranji menunjukkan aktivitas antioksidan dengan menghambat DPPH, superoksida dan hidrogen peroksida. Pada penelitian ini juga menunjukkan kemampuan ekstrak etanolasam keranji mengurangi kemampuan dalam mereduksi besi. Kapasitas total antioksidan ekstrak etanol asam keranji17,66 mg/g yang dibandingkan dengan asam askorbat (Vitamin C). Aktivitas antioksidan yang signifikan dari ekstrak etanol Dialium indum diduga disebabkan oleh adanya senyawa Asam, Flavonoid, Fenol, Saponin, Tannin (senyawa Fenolik) dan Triterpenoid yang ditemukan dalam screening fitokimia sebelumnya.
IDENTIFICATION OF FUNGI IN CAMPUS TOILET BATHROOM WATER Maulida, Rizkha Cahya; Lubis, Syafrina Sari; Hidayat, Muslich
Indonesian Journal of Environmental Sustainability Vol. 1 No. 1 (2023): June 2023
Publisher : Center for Environmental Studies, Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ijes.v1i1.4143

Abstract

The recognition of the health risks associated with fungal contamination in water is increasingly important in terms of microbiological safety and water quality. Fungus is one type of microorganism that can cause disease in humans. As cosmopolitan living things, fungi can be found anywhere close to human existence, be it in water, soil, air, or even in the human body. The purpose of this study was to determine the total number of colonies and the characteristics of pathogenic fungi in bathroom water at UIN Ar-Raniry campus public toilet facilities. This study used bathroom water samples taken at 5 different points. Water samples were taken as much as 0.5 ml and then isolated into Potato Dextrose Agar (PDA) media using the spread plate method. After 7 days of incubation, the total number of colonies was counted and the identification of the fungi was carried out to determine the macroscopic and microscopic characteristics of the fungi. The results of the study of fungal isolation on PDA media were the total colonies obtained from 5 different locations, namely 60 colonies, 47 colonies, 25 colonies, 29 colonies and 46 colonies. The identification results of 31 pure isolates found species namely Aspergillus sp., Aspergillus niger, A. fumigatus, A. flavus, Penicillium sp., and Mucor sp.
ECOPOTENTIAL Bacillus sp. FROM KRUENG ACEH: BIOREMEDIATION AND ANTIMICROBIAL ACTIVITY Rahmah, Annisa; Lubis, Syafrina Sari
Indonesian Journal of Environmental Sustainability Vol. 2 No. 2 (2024): December 2024
Publisher : Center for Environmental Studies, Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ijes.v2i2.5929

Abstract

Bacillus bacteria are bacteria that are tolerant to heavy metal toxicity and are able to reduce heavy metals in the environment by bioaccumulation or bioabsorption. This research aims to determine how resistant Bacillus sp. against the heavy metal Fe, the growth curve of Bacillus sp. affected by Fe metal, characteristics of the Krueng Aceh pathogenic bacteria and the antimicrobial activity of Bacillus sp. against pathogenic microbes. This research method is descriptive and experimental. Based on the research results, it is known that Bacillus sp is resistant to Fe metal at concentrations of 2000 ppm and 2500 ppm. Results of measuring the growth curve of Bacillus sp. exposed to Fe metal with a concentration of 3000 ppm entered the lag phase (adaptation) at hour 0. Then the log (exponential) phase starts from the 12th hour to the 30th hour, then the stationary phase starts from the 30th hour to the 42nd hour. The results of the test for the characteristics of pathogenic bacteria showed that there were 14 isolates of pathogenic bacteria, namely 4 genus Klebsiella, 4 genus Enterobacter, and 6 genus Staphylococcus. The results of the antimicrobial activity test for Bacillus sp., which was tested on pathogenic microbes, namely Klebsiella sp., and Staphylococcus sp., had a weak inhibitory category, namely 2.71, 0.51, this shows that Bacillus sp. unable to inhibit these pathogenic microbes.