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All Journal Public Health of Indonesia Jurnal Ilmu Kesehatan Bhakti Husada: Health Sciences Journal Bali Medika Jurnal Jurnal Kesehatan Rajawali JRKN Pena Dimas Jurnal Teknologi Laboratorium Medik Borneo
Sri Idayani, Sri
Pusat Teknologi Intervensi Kesehatan Masyarakat, Badan Litbang Kesehatan, Kemenkes RI
Religion Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Gambaran Pemeriksaan Telur Cacing Soil Transmitted Helminths pada Pekerja Sampah di TPA Suwung dengan Pewarnaan Kunyit Kering dan Eosin Metode Direct Slide Diani, Ni Luh Ayu Putri Gading; Idayani, Sri; Sudarma, Nyoman
Jurnal Kesehatan Rajawali Vol 15 No 2 (2025): Jurnal Kesehatan Rajawali
Publisher : Sekolah Tinggi Ilmu Kesehatan Rajawali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54350/jkr.v15i2.574

Abstract

Soil-Transmitted Helminths (STH) infections are a significant public health issue in tropical regions, particularly among communities with poor sanitation, such as waste workers at the Suwung Landfill in Denpasar. Helminth egg examination is commonly conducted using the direct smear method with eosin staining; however, synthetic dyes have limitations such as high cost and environmental concerns. This study aimed to compare the staining results of STH eggs in fecal preparations using natural dried turmeric dye and eosin through the slide direct method. This research is descriptive in nature, using a qualitative approach. involving 30 STH-positive fecal samples stained using a dried turmeric extract solution (5 g turmeric powder in 20 mL of 96% ethanol) and eosin. Observation parameters included egg color intensity and background contrast. The results showed that staining with dried turmeric revealed 2 positive samples (13.33%), while eosin staining revealed 5 positive samples (33.3%) for STH eggs. The identified helminth species was Ascaris lumbricoides. Eosin was found to be more effective in highlighting the morphology of helminth eggs. However, dried turmeric still shows potential as a supplementary stain due to its accessibility, affordability, and environmental friendliness.
Identifikasi Malassezia furfur Pada Kerokan Kulit Anak-Anak Di Banjar Dinas Batang Karangasem Bali Adelia, I Gusti Ayu Devi; Bintari, Ni Wayan Desi; Idayani, Sri
Jurnal Kesehatan Rajawali Vol 15 No 2 (2025): Jurnal Kesehatan Rajawali
Publisher : Sekolah Tinggi Ilmu Kesehatan Rajawali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54350/jkr.v15i2.581

Abstract

Tinea versicolor is a common superficial fungal infection found in tropical regions such as Indonesia. This condition is often caused by Malassezia furfur, especially in environments with high temperature and humidity and less than optimal hygiene. This study aimed to identify the presence of M. furfur in elementary school children in Banjar Dinas Batang, Karangasem, Bali, a region with a cool, humid climate and limited sanitation access. The study used a descriptive design with a purposive sampling approach, involving 23 children aged 6–18 years who showed skin lesion symptoms and had not undergone antifungal therapy. Skin scrapings were examined microscopically using a 10% KOH solution combined with Parker ink. Observations were made at 100× and 400× magnifications, identifying the distinctive morphology of M. furfur as a “spaghetti and meatballs” structure. Of the 23 samples examined, 13 (56.5%) tested positive for Malassezia furfur. The majority of cases occurred in boys (83%). Hygiene data showed that 61% bathed once a day, 78% changed clothes twice a day, but 87% washed clothes only once a week. Furthermore, most respondents (56%) used rainwater as their source of clean water. The relatively high prevalence of M. furfur infection reflects the strong influence of the humid environment, inadequate sanitation, and suboptimal hygiene behaviors. Microscopic examination using the KOH and Parker ink method proved effective as an initial screening tool in rural areas. Nevertheless, health education interventions and improved access to clean water are needed to reduce the incidence of skin fungal infections in children
Analisis Hubungan Riwayat Kontak Serumah Dengan Kejadian Tuberkulosis Paru Di RSUD Kabupaten Buleleng Bintari, Ni Wayan Desi; Rejito, Ahmad; Idayani, Sri; Prasetya, Didik
Jurnal Riset Kesehatan Nasional Vol. 10 No. 1 (2026)
Publisher : Institute Teknologi dan Kesehatan (ITEKES) Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37294/jrkn.v10i1.863

Abstract

Infeksi menular tuberculosis paru memiliki angka kejadian yang cukup tinggi di masyarakat. Adanya riwayat kontak khususnya dengan anggota keluarga serumah dilaporkan beresiko dapam peningkatan penularan TB paru. Penelitian ini dilakukan dengan tujuan melakukan analisa terhadap hubungan riwayat kontak serumah dengan kejadian TB paru pada pasien suspek TB di RSUD Kabupaten Buleleng. Jenis penelitian adalah analitik observasional secara cross sectional. Penentuan besaran sampel menggunakan simple random sampling, dimana sampel berjumlah 204 responden suspek TB paru. Data dikumpulkan melalui pengisian kuesioner, wawancara serta pemeriksaan pemeriksaan Tes Cepat Molekuler (TCM) Gene Xpert. Data dilakukan analisa melalui uji univariat serta bivariat. Uji bivariat dilakukan melalui uji Chi-Square. Berdasarkan uji univariat diketahui bahwa dari 204 responden penelitian menunjukkan hasil positif TB paru melalui pemeriksaan TCM. Sementara itu sebanyak 169 responden (82,84%) negatif TB paru. Hasil penelitian menunjukkan bahwa terdapat 34 responden (16,67%) mimiliki resiko riwayat kontak serumah beresiko sedangkan tidak berisiko sebanyak 170 orang (83,33%). Hasil analisis bivariat melalui uji Chi-Square didapatkan besaran nilai p-value < 0,05 sehingga disimpulkan terdapat hubungan bermakna terkait adanya riwayat kontak paparan serumah dengan kejadian TB paru pada pasien di RSUD Kabupaten Buleleng. Upaya skrining kontak serumah, edukasi kesehatan, dan penguatan program contact tracing diperlukan untuk menurunkan risiko penularan TB di masyarakat. Kata Kunci: Gene Xpert, Kontak serumah, Mycobacterium tuberculosis, Test Cepat Molekuler
Co-Authors Abadi, Moh Fairuz Adelia, I Gusti Ayu Devi Adnyani, Widya Dharmawan, Putu Oka Diah Prihatiningsih Diani, Ni Luh Ayu Putri Gading Didik Setiawan Dinar Lubis Dyah Pradnyaparmita Duarsa Lestari, Ni Kadek Yuni Mirayanti, Ni Ketut Ayu Ni Wayan Desi Bintari Ni Wayan Trisnadewi, Ni Wayan Nyoman Sudarma Pamungkas, Mohammad Adreng Pramesti, Theresia Anita Prasetya, Didik Putri, Luh Putu Devani Maeyta Putri, Ni Luh Nova Dilisca Dwi Putu Ayu Indrayathi Rejito, Ahmad Sutrisna Putra, I Gusti Putu Agus Ferry Tandea, Gisel Audina Wati, Ni Made Nopita