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All Journal BULETIN OSEANOGRAFI MARINA Jurnal Sains dan Teknologi Meditory : The Journal of Medical Laboratory Advances in Tropical Biodiversity and Environmental Sciences Journal of Tropical Marine Science Jurnal Media Akuakultur Indonesia Jurnal Perikanan
Kadek Lila Antara
Universitas Pendidikan Ganesha
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Veterinary

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ANTIBACTERIAL ACTIVITY SCREENING OF BACTERIAL ISOLATES ASSOCIATED WITH SEAWEEDS Caulerpa lentillifera AR, Sunthio Barran Cia; Indraningrat, Anak Agung Gede; Widhidewi, Ni Wayan; Wijaya, Made Dharmesti; Antara, Kadek Lila; Choe, Daegeun
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.4051

Abstract

Background: Caulerpa lentillifera is a species of green algae that has been cultivated widely in Indonesia for its nutritional contents and health benefit. This seaweed builds a strong association with bacterial community which play an important role in synthesizing various secondary metabolites including antibacterial activity. Aims:The research aim to explore the symbiotic relationship between C. lentillifera and its associated bacterial community, focusing on the potential of these bacteria to produce antibacterial metabolites.Methods:Bacterial cultivation was performed on three different media namely nutrient agar, plate count agar, and zobell marine agar. The obtained bacterial isolates were identified morphologically and subsequently were screened for their antibacterial activity based on perpendicular cross streak method against Staphylococcus aureus ATCC 25923, Streptococcus mutans FNCC 0405, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 700603. Results: 20 out of 28 bacterial isolates inhibited against at least one of the bacterial targets. Bacterial isolate encoded as NAR9 showed as the most promising bacterial isolates with length inhibition of 19 mm against S. mutans FNCC 0405. Amplification of 16S rRNA gene fragment of the NAR9 bacterial isolate followed by Sanger sequencing showed that the isolate NAR9 has been phylogenetically related to Marinobacter hydrocarbonoclasticus. Conclusion: Overall, this research provides a preliminary result of the untapped potential of bacteria associated with C. lentillifera as the source of antibacterial activity. 
Heavy Metal Controls in Cultivated and Natural Sea Grape (Caulerpa Lentilifera) Purenji , Aura Ramadhanti; Antara, Kadek Lila; Maharani, Made Dwipa Kusuma
Jurnal Media Akuakultur Indonesia Vol 3 No 2 (2023): Indonesian Journal of Aquaculture Medium
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/mediaakuakultur.v3i2.2451

Abstract

Seaweed commodities are one of the products in the fisheries and marine sector that has the potential to be developed. One type is sea grape or commonly known as Caulerna lentillifera which is very widely spread in waters throughout Indonesia. This research method is descriptive quantitative, this research was conducted to determine the heavy metal content in natural sea grapes and cultivated sea grapes. The research procedure consisted of pre-research process to observation and checking of heavy metal content. The results of research on heavy metal content of cultivated sea grapes get good results, while for nature there are levels of heavy metals Pb so it can be said that it is not very good. The results of measuring the length of ramuli in the first week was 3.3 cm, the second week was 4.4 cm then increased in length in the third week to 5.8 cm, and ended in the fourth week with an increase in length of 6.9 cm. The results of the calculation of the number of ramuli in week 1 showed that the average number of ramuli was 12, the average number of ramuli in the first week increased to 14 in the second week and increased in the third week to 17 then ended in the fourth week with an average of 19 ramuli. The quality of water used in the cultivation of sea grapes in Musi Village and natural sea grapes is still in normal status.
Co-Authors AR, Sunthio Barran Cia Aura Ramadhanti Purenji Choe, Daegeun Dwi Setijawati Gede Ari Yudasmara Gede Iwan Setiabudi I Gede Iwan Setiabudi I Nyoman Dodik Prasetia Ida Bagus Jelantik Swasta Indraningrat, Anak Agung Gede Jasmine Masyitha Amelia Kadek Leni Widiartini Ketut Mahardika Ketut Mahardika Made Dharmesti Wijaya Made Dwipa Kusuma Maharani Muhammad Fadjar Ni Luh Putu Ananda Saraswati Ni Nyoman Dian Martini Ni Putu Indah Swardiani Ni Putu Nadya Susanti Purenji , Aura Ramadhanti Putu Justika Nirmala Ardhiana Puspanjali Swardiani, Ni Putu Indah Syahputro, Ichsan Wahyudi, Dicky Ananda Widhidewi, Ni Wayan