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All Journal Medula Biology, Medicine, & Natural Product Chemistry Celebes Biodiversitas : Jurnal Sains dan Pendidikan Biologi Jurnal Ilmu Alam dan Lingkungan JMM (Jurnal Masyarakat Mandiri) JURNAL PengaMAS Jurnal Kreativitas PKM JPBIO (Jurnal Pendidikan Biologi) Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat JCPS (Journal of Current Pharmaceutical Sciences) Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Pengabdian Masyarakat Khatulistiwa Journal SAGO Gizi dan Kesehatan Jurnal Abdimas Sangkabira Molucca Medica Society: Jurnal Pengabdian Masyarakat PAMERI : Pattimura Medical Review MEDULA Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo
Eka Astuty
Faculty Of Medicine, Universitas Pattimura, Ambon, Indonesia
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Biochemical identification of endophytic bacteria isolates from medicinal plant kayu jawa lannea coromandelica (houtt.) merr Eka Astuty; Indas Wari Rahman; Melda Yunita
JPBIO (Jurnal Pendidikan Biologi) Vol 8, No 1 (2023): April 2023
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v8i1.2095

Abstract

Medicinal plants and their endophytes are a source of valuable bioactive compounds and their secondary metabolites which contribute to more than 80% of natural medicines available in the market. A plant that has long been used as a traditional medicinal herb for healing is the Kayu Jawa (Lannea coromandelica (Houtt) Merr). This study aimed to characterization and identification of endophytic bacterial isolates isolated from medicinal plants Kayu Jawa (L. coromandelica (Houtt.) Merr). This research is a laboratory experimental research. In total, 8 isolates were selected as representatives of the bacterial community living in the tissues of Kayu Jawa (L. coromandelica (Houtt.) Merr). The microscopic observation was performed by Gram staining. Biochemical identification was conducted using Vitek 2. The results showed that Bacillus was the dominant genus in this study followed by Staphylococcus and Pantoea. Based on the research that has been done, it can be concluded that the endophytic bacteria isolated from Kayu Jawa Lannea coromandelica (Houtt) Merr, six isolates belonging to Bacillus and another two belonging to Staphylococcus and Pantoea genera. However, for the exploration of this prospective application, it is very important to do a systematic study of the biological and distribution of endophytic bacteria in the host.
UPAYA PENCEGAHAN DAN PENANGANAN AWAL DIARE MELALUI PENYULUHAN PADA SISWA SMP DI NEGERI LAHA AMBON Elpira Asmin; Eka Astuty; Ericka Mohty Septania Sely
Jurnal Abdimas Sangkabira Vol. 3 No. 2 (2023): Jurnal Abdimas Sangkabira, Juni 2023
Publisher : Program Studi Diploma III Akuntansi Fakultas Ekonomi dan Bisnis Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdimassangkabira.v3i2.565

Abstract

Di Indonesia, diperkirakan diare menyebabkan 10-24 kematian per 100.000 orang pada tahun 2016, sementara puncak kematian terjadi pada orang dewasa yang berusia di atas 70 tahun, dengan 200-299 kematian per 100.000 orang. Berdasarkan observasi, survei,  dan pengambilan data profil kesehatan yang telah dilakukan pada penduduk Negeri Laha tentang  riwayat  penyakit  yang pernah  dialami  oleh  masyarakat  Negeri  Laha, ditemukan bahwa  sebanyak  198  orang  pernah  mengalami  Diare. Kegiatan pengabdian berupa penyuluhan dengan metode ceramah dikombinasikan dengan sesi tanya jawab serta dilengkapi dengan pengisian kuesioner pre post diakhiri dengan pembagian leaflet tentang diare. Sasaran kegiatan adalah siswa Sekolah Menengah Pertama (SMP) LKMD Laha. Terjadi peningkatan pengetahuan tentang diare pada siswa SMP LKMD Laha setelah diberikan penyuluhan Diharapkan setelah mengikuti penyuluhan, pengetahuan siswa meningkat dan dapat membiasakan perilaku hidup yang bersih dan sehat untuk mencegah terjadinya diare.
PEMBERDAYAAN MASYARAKAT MELALUI EDUKASI MANFAAT PROBIOTIK DAN PELATIHAN PEMBUATAN MINUMAN TEH KOMBUCHA Melda Yunita; Juen Carla Warella; Eka Astuty; Elpira Asmin; Morgan Ohiwal
JMM (Jurnal Masyarakat Mandiri) Vol 8, No 2 (2024): April
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v8i2.21658

Abstract

Abstrak: Teh Kombucha merupakan salah satu minuman probiotik yang memiliki manfaat bagi Kesehatan karena mengandung berbagai jenis vitamin, antibakteri, dan antioksidan. Negeri Lonthoir merupakan salah satu daerah yang masyarakatnya belum mengetahui manfaat teh Kombucha sebagai minuman probiotik yang berguna bagi Kesehatan. Kegiatan ini bertujuan untuk meningkatkan pengetahuan masyarakat mengenai manfaat probiotik dan meningkatkan keahlian dalam membuat teh Kombucha. Kegiatan dilakukan pada 27 September 2023 dengan melibatkan 18 anggota masyarakat. Tahapan kegiatan dimulai dengan memberukan pretest, edukasi secara lisan, posttest dan demonstrasi. Hasil analisis menunjukkan terjadi peningkatan pengetahuan sebesar 71,33%. Disimpulkan bahwa kegiatan ini memberikan dampak yang sangat berarti bagi masyarakat dalam meningkatkan pengetahuan dan menambah softskill dalam membuat teh Kombucha secara mandiri.Abstract: Kombucha tea is a probiotic beverage with health benefits because it contains various types of vitamins, antibacterial agents, and antioxidants. Lonthoir is one of the areas where people need to learn the health benefits of kombucha tea as a probiotic beverage. This activity aimed to increase the community knowledge about the benefits of probiotics and improve their skills in making Kombucha tea. The activity was conducted on September 27, 2023, with 18 community members participating. The phases of the activity began with the administration of a pretest, oral education, posttest, and demonstration. The results of the analysis showed a 71.33% increase in knowledge. It was concluded that this activity had a significant impact on the community in terms of increasing knowledge and adding soft skills in making kombucha tea independently.
Analisis mikrobiologis jajanan makanan di Kantin Pujasera Universitas Pattimura Ambon Eka Astuty; Andi Wahyuli; Farah Christina Noya
Jurnal SAGO Gizi dan Kesehatan Vol 6, No 1 (2025): April
Publisher : Poltekkes Kemenkes Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/gikes.v6i1.1956

Abstract

Background: Snack food is typically prepared and served in open spaces, such as in food courts or by the roadside, which makes it highly susceptible to bacterial contamination. If consumed in certain amounts, these bacteria can cause foodborne illnesses (foodborne diseases).Objective: This study aims to assess the quality of food sold at the Pujasera Canteen of Pattimura University, Ambon, based on microbiological parameters by calculating the Total Plate Count (TPC).Method: This research is a descriptive quantitative study with an observational approach. The samples used consisted of five types of snack foods: noodle with meatballs, egg noodles, chicken rice, fried tubers, and fried rice, which were randomly sampled from sales stands. The study was conducted in May 2024 at the Microbiology Laboratory of the Faculty of Medicine, Pattimura University. Statistical analysis was carried out by calculating the total colonies using the formula TPC (Total Plate Count).Results: The results showed the TPC values for sample A at 1 × 10^6 colonies/g, sample B at 2,5 × 10^7 colonies/g, sample C at 1,2 × 10^7 colonies/g, sample D at 7,3 × 10^7 colonies/g, and sample E at 4,6 × 10^4 colonies/g. Based on these values, four out of the five samples did not meet the criteria set by BPOM (Indonesia’s National Agency of Drug and Food Control), referring to SNI 7388:2009, as they exceeded the maximum microbial contamination limit of <1 × 10^5 colonies/g. Only one sample met the criteria.Conclusion: Four out of the five samples were categorized as having poor quality, as the microbial count exceeded the maximum contamination limit set by SNI. 
Anticandidal Effects of Ethanolic Extract of Lada Katokkon (Capsicum annuum chinense) Against Emerging Fungal Pathogens Allen Mangoting; Eka Astuty; Amanda Gracia Manuputty
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1501-1507

Abstract

Candidiasis is an opportunistic mycotic infection with a high prevalence, caused by Candida species, especially Candida albicans, Candida tropicalis, and Candida parapsilosis. The increasing resistance of Candida spp. to conventional antifungals such as fluconazole poses a challenge in the management of candidiasis, thus the need for natural-based alternatives, such as Lada Katokkon. This condition encourages the need to explore alternative therapeutic agents based on natural ingredients. Lada Katokkon (Capsicum annuum chinense Jacq), a local chili variety cultivated in the Toraja region, is known to contain secondary metabolite compounds such as capsaicin, flavonoids, saponins, and alkaloids that have potential as antifungals. This study aims to evaluate the antifungal activity of ethanol extract of Lada Katokkon against the growth of C. albicans, C. tropicalis, and C. parapsilosis in vitro using disc diffusion method. The extract was tested at five concentrations: 1%, 3%, 5%, 7%, and 9%. There was a positive relationship between extract concentration and inhibition zone diameter. The highest activity was recorded at 9% concentration, particularly against C. parapsilosis (16.89 ± 4.74 mm), followed by C. albicans (9.03 ± 5.96 mm) and C. tropicalis (8.07 ± 0.81 mm). In contrast, 1% concentration produced the lowest zone of inhibition in all species tested. These results suggest that ethanol extract of Lada Katokkon has potential as an alternative natural antifungal agent in candidiasis therapy.
Antibacterial Activity and Phytochemical Screening of Ethanol Extract of Cashew Leaves A. occidentale L. Against Escherichia coli Eka Astuty; Rolan Aponno; Is I. Hataul
MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo Vol. 10 No. 1 (2022): Oktober
Publisher : MEDULA: Jurnal Ilmiah Fakultas Kedokteran Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: All parts of the cashew tree Anacardium occidentale L. (especially the leaves and bark) have been widely used as traditional herbal medicines, and are used throughout the world. Thus, in recent decades, the traditional medicinal properties of the Anacardium plant, and its various biological effects are being studied extensively. Purposes: This study aimed to conduct a preliminary study of the antibacterial properties and phytochemical screening of the ethanol extract of cashew leaves. Methods: The antibacterial test of was determined by the Kirby-Bauer disc paper diffusion method. Qualitative test of phytochemical content was carried out using chemical reagents. Results: The results indicated that the ethanol extract of cashew leaves has an inhibitory effect on the growth of Escherichia coli. The smallest inhibition zone was found in the extract with a concentration of 10% of 5.75 mm and the largest was found at a concentration of 100% with a diameter of 14 mm. The phytochemical screening revealed that extracts contained; alkaloids, flavonoids, steroids, terpenoids, phenolics, saponins, and tanins Conclusions: The ethanol extract of cashew leaves was able to inhibit the growth of Escherichia coli test bacteria at all concentration variations. Phytochemical screening also found that the ethanol extract of cashew leaves contains alkaloids, flavonoids, steroid terpenoids, phenolics, saponins, and tannins which are known to have antibacterial properties.
Co-Authors Alessandra Flowrence Saija Allen Mangoting Amanda Gracia Manuputty Amanda Gracia Manuputty Andi Wahyuli Anugrah P.M.D. Kamoda Astrid S. W. Sumah Astuti Nur Fadhilah Bertha Jean Que Dessri Wahyuni Elpira Asmin Elpira Asmin Ericka Mohty Septania Sely Etrin Z. E. Saputra Linggar Farah Christina Noya Halidah Rahawarin Hasna Vuspita Latuconsina Ian Risaldy Tofure Indas Wari Rahman Indrawanti Kusadhiani Irwan Irwan Is I. Hataul Is Ikhsan Hataul Laura B S Huwae Maisya Zahra Al Banna Maria Nindatu Melda Yunita Muhammad Zaid Wakano Nindatu, Maria Nurul H D J F Ahmad Ohiwal, Morgan Ony Wibriono Angkejaya Rolan Aponno Sukmawati Sukmawati Sukmawaty, Eka Sulfiana , Tahitu, Ritha Taihuttu, Yuniasih MJ Warella, Juen Carla Widya Putri Ramadhani Yuniasih M.J. Taihuttu Zulkarnain Zulkarnain Zuraidah Zuraidah