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Uji Daya Hambat Ekstrak Etanol Daun Tapak Kuda Ipomoea pes-caprae Terhadap Pertumbuhan Bakteri Propionibacterium acne Etrin Z. E. Saputra Linggar; Eka Astuty; Yuniasih M.J. Taihuttu
Jurnal Ilmu Alam dan Lingkungan Vol. 12 No. 1 (2021): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v12i1.13094

Abstract

Ipomoea pes-caprae is a plant that has the ability as an anti-bacterial. The active compounds of Tapak Kuda leaves as anti-bacteria are flavonoids, alkaloids, and tannins. The purpose of this study was to determine the ability of the ethanol extract of tapak kuda leaves to inhibit the growth of Propionibacterium acne bacteria. This research is a laboratory experimental study using the disc diffusion method and using various extract concentrations of 5%, 10%, 25%, 50%, and 100%. The ethanol extract of tapak Kuda leaves was obtained from the maceration process of tapak Kuda leaves with ethanol solvent, then tested the inhibition of Propionibacterium acne. The parameters observed were the clear zone formed on the growth of Propionibacterium acne bacteria. The results showed that the ethanol extract of tapak Kuda leaves had a low ability to inhibit the growth of Propionibacterium acne bacteria. The clear zone formed at a concentration of 100% was only 1.14 mm while at a concentration of 5%, 10%, 25% and 50% there was no clear zone formed.
Edukasi Manfaat Yogurt Sebagai Salah Satu Probiotik Dan Metode Pembuatan Yogurt Sederhana Eka Astuty; Melda Yunita; Astuti Nur Fadhilah
JURNAL KREATIVITAS PENGABDIAN KEPADA MASYARAKAT (PKM) Volume 4 Nomor 1 Februari 2021
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v4i1.3535

Abstract

ABSTRAK Yogurt adalah produk fermentasi bakteri dengan menggabungkan efek Lactobacillus delbrueckii subspesies bulgaricus dan Streptococcus salivarius subspesies thermophilus. Kaitan ilmiah antara probiotik dan manfaatnya bagi kesehatan manusia pertama kali diungkapkan oleh ahli mikrobiologi Rusia bernama Metchnikoff (1907). Ia menyatakan bahwa asam laktat yang dihasilkan oleh Lactobacillus dalam yoghurt dapat menghambat pertumbuhan beberapa spesies bakteri patogen.Masyarakat  perlu  mengetahui  lebih  dalam  mengenai  manfaat  yoghurt sebagai salah satu minuman probiotik . Yoghurt dapat diproduksi dalam skala besar maupun kecil baik untuk keperluan komersial maupun untuk konsumsi rumah tangga. Kegiatan pengabdian kepada masyarakat  ini  mencakup  kegiatan edukasi tentang manfaat  yoghurt  dan  cara  pembuatannya  kepada  masyarakat dan diharapkan masyarakat  dapat  meningkatkan  derajat  kesehatannya  secara  mandiri Kata Kunci :probiotik, yogurt, edukasi  ABSTRACT Yogurt is a bacterial fermentation product by combining the effects ofLactobacillus delbrueckii subspecies bulgaricus and Streptococcus salivarius subspecies thermophilus. The scientific link between probiotics and their benefits to human health was first revealed by a Russian microbiologist named Metchnikoff (1907). He stated that the lactic acid produced by Lactobacillus in yogurt can inhibit the growth of several species of pathogenic bacteria. People need to know more about the benefits of yogurt as a probiotic drink. Yogurt can be produced on a large or small scale both for commercial use and for household consumption. This community service activity includes educational activities about the benefits of yogurt and how to make it to the community and it is hoped that the community can improve their health status independently. Keywords: probiotic, yogurt, education
Antibiotic susceptibility analysis of Escherichia coli as the cause of urinary tract infection Melda Yunita; Hasna Vuspita Latuconsina; Eka Astuty; Alessandra Flowrence Saija
JPBIO (Jurnal Pendidikan Biologi) Vol 7, No 2 (2022): November 2022
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v7i2.1849

Abstract

The incidence of urinary tract infection (UTI) in Indonesia is still quite high. The UTIs are generally treated with antibiotic. However, the use of antibiotics has recently increased tremendously thus triggering bacterial resistance. This study was to analyze the susceptibility of the Escherichia coli as the cause of the UTI to 15 antibiotics at the UPTD Health Laboratory Center of Maluku Province. The study was an experimental laboratory study using the disk diffusion method. The antibiotic disc was placed on the media and the inhibition zone against E. coli was measured. The results showed that the E. coli was resistant (26.67%) to 4 types of antibiotics, namely amoxilin, ampicillin, sulfametazole dan penicillin. Meanwhile, E. coli was sensitive (46.66%) to 7 types of antibiotics, namely kanamycin, gentamicin, subactam ampicillin, chloramphenicol, amikacin, ceffazedine and azithromycin. Furthermore, E. coli was intermediate (26.67%) against 4 types of antibiotics, namely ciprofloxacin, ceptriaxone, cefoxitin and colistin. This study concluded that of the 15 antibiotics tested, gentamicin, amikacin, and kanamycin were the most effective antibiotics in inhibiting the growth of E. coli that causes UTI. Nevertheless, the appropriate choice of antibiotic needs to be adjusted only by physicians based on local, regional and national patterns of susceptibility.
Antibacterial effect of ethanol extract of nutmeg against foodborne disease pathogens Eka Astuty; Eka Sukmawaty
JPBIO (Jurnal Pendidikan Biologi) Vol 7, No 2 (2022): November 2022
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v7i2.1856

Abstract

Foodborne disease is a disease that occurs as a result of food contamination by microbes or harmful chemicals. Nutmeg (Myristica fragrans Houtt.)  is widely used as a spice and flavor enhancer for food and beverages. In addition, it has traditionally been used to treat diarrhea and kidney disease. Several pharmacological studies have reported the antioxidant, antimicrobial, antidiarrheal and anti-inflammatory activity of this plant. Antibacterial effectiveness test of nutmeg against several pathogens in this study was carried out using the disc diffusion method. The results of this study indicated that the ethanol extract of flesh of fruit (concentration of 5%, 10%, 15%, 20%, and 25%) had antibacterial activity against all tested pathogenic bacteria. Different results were shown by the ethanol extract of seed and mace. All the ethanol extract concentrations of seed were not able to inhibit the growth of Shigella sp. and Clostridium sp., while the ethanol extract of mace in all concentrations was unable to inhibit the growth of Shigella sp. Various factors may affecting this extract and compound activity when used in complex biological systems such as in vivo and human studies. Future studies must also focus on aspects of pharmacokinetics and toxicological plant extracts and phytochemicals.
Antifungal Activity of Kinar (Kleinhovia hospita L.) Leaf Ethanol Extract Against Malassezia furfur Muhammad Zaid Wakano; Eka Astuty; Amanda Gracia Manuputty
Folia Medica Indonesiana Vol. 58 No. 4 (2022): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (894.751 KB) | DOI: 10.20473/fmi.v58i4.38387

Abstract

Highlights : Alternative medicine is needed for pityriasis versicolor because there are recurrence cases and resistance to antifungal agents. Kinar leaf ethanol extract cannot inhibit the growth of Malassezia furfur. Further studies are suggested on the type of solvent and appropriate concentration to attract more bioactive compounds. Abstract : Pityriasis versicolor is a superficial dermatomycosis that can decrease human self-confidence. This infection is caused by the fungus Malassezia furfur. Eighty percent of recurrence cases after treatment and resistance to antifungal agents were found. Therefore, alternative medicine is needed. Kinar (Kleinhovia hospita Linn.) is a tropical plant that have bioactive compounds, such as alkaloids, flavonoids, tannins, and saponins. The purpose of this study was to determine the effectiveness of kinar leaf extract against the growth of Malassezia furfur. This research was a laboratory experimental study using paper disc diffusion method. Kinar leaves (green and yellow leaves) were macerated using 96% ethanol and made into concentrations of 10%, 20%, 40%, 60%, 80%, and 100%. As much as 200 mg of ketoconazole was used as a positive control and distilled water as a negative control then tested on Malassezia furfur using Sabouraud dextrose agar (SDA). The parameter observed was the clear zone formed around the paper disc. The tests and observations showed that there was a clear zone formed around the paper disc. It means that the kinar leaf extract cannot inhibit the growth of Malassezia furfur.
Aktivitas Antibakteri dan Skrining Fitokimia Ekstrak Etanol Daun Jambu Mete Anacardium occidentale L. Terhadap Pertumbuhan Escherichia coli Antibacterial Activity and Phytochemical Screening of Ethanol Extract of Cashew Leaves Anacardium occidentale L. Against Escherichia coli Eka Astuty
MEDULA (JURNAL ILMIAH FAKULTAS KEDOKTERAN UNIVERSITAS HALU OLEO) Vol 10, No 1 (2022)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46496/medula.v10i1.26112

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ABSTRACTBackground: All parts of the cashew tree Anacardium occidentale L. (especially the leaves and bark) have been widely used as traditional herbal medicines, and are used throughout the world. Thus, in recent decades, the traditional medicinal properties of the Anacardium plant, and its various biological effects are being studied extensively. Purpose(s): This study aims to conduct a preliminary study of the antibacterial properties and phytochemical screening of the ethanol extract of cashew leaves. Methods: The antibacterial test of was determined by the Kirby baeur disc paper diffusion method. Qualitative test of phytochemical content was carried out using chemical reagents. Results: The results indicated that the ethanol extract of cashew leaves has an inhibitory effect on the growth of Escherichia coli. The smallest inhibition zone was found in the extract with a concentration of 10% of 5.75 mm and the largest was found at a concentration of 100% with a diameter of 14 mm. The phytochemical screening revealed that extracts contained; alkaloids, flavonoids, steroids, terpenoids, phenolics, saponins, and tanins Conclusion: The ethanol extract of cashew leaves was able to inhibit the growth of Escherichia coli test bacteria at all concentration variations. Phytochemical screening also found that the ethanol extract of cashew leaves contains alkaloids, flavonoids, steroid terpenoids, phenolics, saponins, and tannins which are known to have antibacterial properties.Keywords: cashew, ethanol extract, antibacteria ABSTRAKLatar Belakang: Semua bagian pohon jambu mete Anacardium occidentale L. (terutama daun dan kulit batang) telah banyak digunakan sebagai obat herbal tradisional, dan digunakan di seluruh dunia. Dengan demikian, dalam beberapa dekade terakhir, sifat obat tradisional tanaman Anacardium, dan berbagai efek biologis sedang dipelajari secara ekstensif Tujuan: Penelitian ini bertujuan untuk melakukan studi pendahuluan sifat antibakteri dan skrining fitokimia dari ekstrak etanol daun jambu mete. Metode: Uji antibakteri ekstrak ditentukan dengan metode difusi kertas cakram Kirby baeur. Uji kualitatif kandungan fitokimia dalam ekstrak dilakukan dengan pereaksi kimia. Hasil: Ekstrak etanol daun jambu mete memiliki daya hambat terhadap pertumbuhan Escherichia coli. Zona hambat terkecil terdapat pada ekstrak dengan konsentrasi 10% sebesar 5,75 mm dan terbesar terdapat pada konsentrasi 100% dengan diameter 14 mm. Hasil skrining fitokimia menunjukkan bahwa ekstrak etanol daun jambu mete mengandung senyawa golongan alkaloid, flavonoid, steroid, terpenoid, fenolik, saponin, dan tanin Simpulan: Ekstrak etanol daun jambu mete mampu menghambat pertumbuhan bakteri uji Escherichia coli pada semua variasi konsentrasi. Skrining fitokimia juga menemukan bahwa dalam ekstrak etanol daun jambu mete mengandung alkaloid, flavonoid, steroid terpenoid, fenolik, saponin, dan tannin yang diketahui memiki kemampuan sebagai antibakteriKata Kunci: jambu mete, ekstrak etanol, antibakteri
Pelatihan Sterilisasi Alat Dan Bahan Medis Pada Anggota Tim Bantuan Medis Vertebrae Fakultas Kedokteran Universitas Pattimura Eka Astuty; Ony Wibriono Angkejaya
Society : Jurnal Pengabdian Masyarakat Vol 1, No 5 (2022): September
Publisher : Edumedia Solution

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55824/jpm.v1i5.137

Abstract

The Vertebrae Medical Assistance Team (TBM) of the Faculty of Medicine, University of Pattimura is a student organization that has many work programs, and one of their work programs is community service. Medical students will become doctors who will take part in society and who are not only required to be capable and competent in solving all health problems, but also must be able to become examples and role models in moving healthy life for the community. As part of the TBM, medical students are required to have basic medical assistance competencies, therefore, the purpose of this activity is to provide detailed knowledge of sterilization and disinfection techniques that have been neglected so far, starting with simple basic knowledge. In general, the implementation methods used in this community service are sterilization education, sterilization objectives, types of sterilization, and the practice of using autoclave sterilizers. This community service activity has achieved the main planned objectives, namely increasing the knowledge and understanding of participants through sterilization training for medical equipment and materials as part of the program to increase the competence of TBM members. There are three main components discussed in the understanding and skills of participants, namely understanding the definition and purpose of sterilization, understanding the types of sterilization, and being able to practice how to use autoclave sterilizers
ANALISIS LEVEL PENGETAHUAN MASYARAKAT TERHADAP PERILAKU HIDUP BERSIH DAN SEHAT UNTUK MENCEGAH TRANSMISI COVID-19 Melda Yunita; Morgan Ohiwal; Eka Astuty; Sukmawati Sukmawati
JMM (Jurnal Masyarakat Mandiri) Vol 6, No 5 (2022): Oktober
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v6i5.9875

Abstract

Abstrak: Serangan dari wabah novel coronavirus (Covid-19) telah menyebar secara masiv di seluruh wilayah Indonesia dan pernah menjadi kasus dengan angka kematian tertinggi di Asia tenggara. Berbagai upaya telah dilakukan oleh pemerintah bersama dengan pihak terkait untuk mencegah transmisi Covid-19 yang sangat cepat, termasuk dengan mempromosikan perilaku hidup bersih dan sehat (PHBS) pada seluruh lapisan masyarakat, khususnya di Desa Air Salobar Ambon. Tujuan dari kegiatan ini adalah untuk menganalisis level pengetahuan masyarakat mengenai PHBS untuk mencegah transmisi Covid-19. Kegiatan ini dilaksanakan pada 20 Maret 2021 di Desa Air Salobar dengan melibatkan 26 responden. Metode yang digunakan pada kegiatan ini ialah metode deskriptif yaitu dengan memberikan pretest, edukasi berupa penyuluhan PHBS, dan posttest kepada masyarakat. Hasil analisis menunjukkan bahwa nilai posttest meningkat dari 40,8% menjadi 95% setelah diberikan edukasi dengan rata-rata peningkatan nilai posttest dibandingkan pretest ialah sebesar 56.2%. Dapat disimpulkan bahwa edukasi membrikan dampak yang signifikan terhadap peningkatan level pengetahuan masyarakat mengenai PHBS setelah mendapat edukasi dari fasilitator.Abstract: The attack from novel coronavirus (Covid-19) outbreak has spread massively throughout Indonesia and has ever become the case with the highest death rate in Southeast Asia. Various efforts have been conducted by the government together with related parties to prevent the very rapid transmission of Covid-19, including by promoting clean and healthy living behavior (PHBS) at all levels of society, particularly in Air Salobar Village, Ambon. The objective of the community service activity was to analyze the level of knowledge of the community on PHBS to prevent the transmission of Covid-19. This activity was carried out on March 20, 2021 in Air Salobar Village by involving 26 respondents. The method used was a descriptive method, by providing pretest, education in the form of PHBS counseling, and posttest to the respondents. The results of the analysis showed that the posttest score increased from 40.8% to 95% after being given education with an increase in knowledge level of 56.2%.
EDUKASI RAGAM TANAMAN OBAT KELUARGA DI DUSUN MAHIA, MALUKU Eka Astuty; Elpira Asmin; Melda Yunita
JMM (Jurnal Masyarakat Mandiri) Vol 6, No 6 (2022): Desember
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v6i6.11055

Abstract

Abstrak:Tanaman Obat Keluarga (TOGA) pada hakekatnya adalah tanaman berkhasiat yang ditanam di lahan pekarangan yang dikelola oleh keluarga. Mitra kegiatan pengabdian masyarakat ini adalah masyarakat dusun Mahia. Tujuan dari kegiatan pengabdian masyarakat ini adalah untuk mengedukasi dan menganalisis tingkat pengetahuan masyarakat dusun Mahia tentang ragam tanaman obat keluarga. Edukasi disampaikan dengan metode ceramah menggunakan slide presentasi tentang jenis-jenis TOGA, manfaat TOGA, kemudian menampilkan video tata cara menanam TOGA serta pengelolaan juga pengolahan TOGA. Perbandingan hasil pre test dan post test pada kegiatan ini menunjukkan adanya peningkatan pengetahuan peserta tentang TOGA. Peningkatan pengetahuan tentang pengelompokan TOGA dan bagian tanaman yang dapat dijadikan obat adalah sebesar 38% sedangkan peningkatan pengetahuan tentang cara pengolahan TOGA adalah sebesar 34,3%. Evaluasi dalam bentuk post test meskipun bisa menunjukkan adanya peningkatan pengetahuan, namun peserta kegiatan belum bisa mengaplikasikan secara langsung pengetahuan yang diperoleh. Oleh karena itu, sebagai rencana tindak lanjut, tim pengabdian masyarakat merencanakan membuat kegiatan pengabdian masyarakat di kesempatan berikutnya tentang pembuatan simplisia dan budidaya TOGA.Abstract:Family Medicinal Plants (TOGA) are essentially nutritious plants that are planted in the yard managed by the family. The partners of this community service activity are the Mahia community. The purpose of this community service activity is to educate and analyze the level of knowledge of the Mahia community about the variety of family medicinal plants. The education was delivered using a lecture method using presentation slides about the types of TOGA, the benefits of TOGA, then showing a video on how to plant TOGA and the management and processing of TOGA. The comparison of the results of the pretest and posttest in this activity showed an increase in participants' knowledge about TOGA. The increase in knowledge about TOGA grouping and plant parts that can be used as medicine is 38%, while the increase in knowledge about TOGA processing is 34.3%. Evaluation in the form of a post test, although it can show an increase in knowledge, but the activity participants have not been able to directly apply the knowledge gained. Therefore, as a follow-up plan, the community service team plans to conduct community service activities in the next opportunity regarding the manufacture of simplicia and TOGA cultivation.
UJI AKTIVITAS ANTIOKSIDAN ALGA COKELAT Saragassum sp. DENGAN METODE 1,1- DIFENIL-2-PIKRIHIDRASIL (DPPH) Anugrah P.M.D. Kamoda; Maria Nindatu; Indrawanti Kusadhiani; Eka Astuty; Halidah Rahawarin; Elpira Asmin
PAMERI Vol 3 No 1 (2021)
Publisher : Fakultas Kedokteran Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.698 KB) | DOI: 10.30598/pamerivol3issue1page60-72

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Antioxidants are chemical compounds that are naturally present in the human body, which can donate hydrogen atoms to free radicals, resulting in chain reactions and converting free radicals into stable forms. Based on the source, antioxidants can be divided into 2 namely natural antioxidants and synthetic antioxidants that can overcome the formation of free radicals. One of the natural ingredients that can overcome the formation of free radicals is the brown algae Sargassum sp. The purpose of this study was to test antioxidant activity using the 1,1-Diphenyl-2-Pikrihydrasil (DPPH) method. This research is a type of experimental laboratory research. The research methodology included and prepared ingredients, extract preparation, and antioxidant activity testing using the DPPH method. Data analysis in this study was carried out by calculating the percent (%) of activity obtained from the absorbance of the data from each concentration. After obtaining the percent (%) antioxidant activity data for each absorbance sample, the IC50 value is calculated using a non-linear regression equation by substituting y = ax + b which states the relationship between log concentration and percent (%) antioxidant activity (inhibition). The results showed 50% concentration inhibition (IC50) of brown algae Sargassum sp. is 5,864. So it can be conclude that the algae Sargassum sp. has a very active antioxidant activity.