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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Bioscience Jurnal Bioteknologi & Biosains Indonesia
Febri Doni
Department Of Biology Faculty Of Mathematics And Natural Sciences Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Immunology & microbiology

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Respon Pertumbuhan Benih Padi Varietas Anak Daro Asal Solok Terhadap Isolat Trichoderma Indeginous Azwir Anhar; Dwi HIlda Putri; Febri Doni; Linda Advinda
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.885 KB) | DOI: 10.24036/0202041108377-0-00

Abstract

Trichoderma spp adalah jamur non patogen yang berasosiasi dengan rhizosfer dan daun tanaman. Jamur ini juga mampu mengendalikan penyakit dan menghasilkan zat pengatur tumbuh, sehingga potensial  digunakan dalam pertaniankhususnya dalam budidaya pertanian organik.  Penelitian  kemampuan Trichoderma spp dalam meningkatkan pertumbuhan benih padi masih terbatas. Penelitian ini, melaporkan kemampuan Trichoderma setempat untuk meningkatkan poertumbuhan kecambah  benih padi. Hasil penelitian menunjukkan bahwa isolat Trichoderma mampu meningkatkan daya kecambah dan vigor benih padi. Hasil penelitian membuktikan bahwa isolat Trichoderma indegenous memiliki potensi untuk digunakan sebagai pengatur pertumbuhan benih padi. Hasil penelitian meningkatkan pemahaman lebih lanjut tentang peranan jamur dalam meningkatkan pertumbuhan padi
Prospecting the roles of Trichoderma in sustainable crop production: biotechnological developments and future prospects Rizky Riscahya Pratama Syamsuri; Dwi Astuti Aprilia; Atasya Yasmine Fakhira; Almira Salma Nabilah; Sulistya Ika Akbari; Nia Rossiana; Febri Doni
Bioscience Vol 6, No 2 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202262119346-0-00

Abstract

The filamentous fungal genus Trichoderma are reported to have a significant impact on the growth and development of various crops. Trichoderma species which are residing in the rhizosphere of crops, and as fungal symbionts living within plant tissues have multiple roles in enhancing crops’ agronomic traits, fitness, growth and yield, and in modulating their tolerance towards biotic and abiotic stresses. This article discusses on the potential and impact of Trichoderma in improving the development and production of crops, as well the mechanism of Trichoderma in improving the development and production of crops. This article also highlights the ability of Trichoderma for improving crops’ tolerance to abiotic and biotic stresses. Prospectively, the use of Trichoderma inoculants offers some new, cost-effective, and more eco-friendly practices for increasing crops’ production.
Antibacterial Activities of Red Mangrove (Rhizophora stylosa Griff.) Leaf Extract against Klebsiella pneumoniae ATCC 700603 Kalasuba, Karina; Rahayuningsih, Sri Rejeki; Amalia, Eri; Syaputri, Yolani; Doni, Febri; Rustama, Mia Miranti
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.661-669

Abstract

Multidrug-resistant (MDR) Klebsiella pneumoniaeis a critical pathogen causing severe human diseases, including pneumonia. Combating the growing threat of MDRK. pneumoniae requires innovative approaches, such as exploring plant-derived antibacterial agents. Rhizophora stylosa Griff., a mangrove species with traditional medicinal uses, is recognized for its bioactive compounds with potential antibacterial properties. However, research on its bioactive constituents remains limited. This study investigated the antibacterial activity of R. stylosa leaf extracts prepared via maceration and liquid-liquid fractionation against K. pneumoniae ATCC 700603. Using agar-well diffusion and cell leakage assays, the water fraction demonstrated moderate inhibition of K. pneumoniae, producing an average inhibition zone of 8.24 mm and a minimum inhibitory concentration (MIC) of 400 mg/ml. UV-Vis spectrophotometry revealed that the water fraction disrupted protein and nucleic acid synthesis, evidenced by leakage of cellular materials at 260 and 280 nm. Additionally, scanning electron microscopy (SEM) images of K. pneumoniae cells treated with the water fraction showed pore formation and structural damage. These results emphasize the antibacterial potential of the water fraction of R. stylosa leaves against MDRK. pneumoniae. Further investigations are necessary to isolate and identify the specific bioactive compounds responsible for these effects. Moreover, comprehensive assessments of activity and toxicity are crucial to advance R. stylosa-based antibacterial agents as promising alternatives for addressing the challenge of MDR bacterial infections.
Trichoderma spp. isolates stimulate rice seedling growth of Sertani 13 variety Akbari, Sulistya Ika; Prismantoro, Dedat; Rossiana, Nia; Doni, Febri
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 1 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i1.50849

Abstract

Trichoderma has become one of the most studied filamentous fungi to be used as a greener and more sustainable solution for improving the production and growth of numerous crops, due to its capability to form symbiotic associations with plants. This study aimed to evaluate the effect of Trichoderma isolates obtained from the rhizosphere of organic rice fields in Sukabumi, Indonesia, in enhancing rice germination and seedling growth. A laboratory experiment used a completely randomized design consisting of seed treatments of 21 Trichoderma isolates (T1-T21) and a control treatment without Trichoderma (C). The inoculation was employed to elucidate any potential effects of Trichoderma isolates. Results showed that five isolates, i.e., T5, T7, T9, T10, and T14 stimulated the highest seedling vigor index, root and shoot length, and fresh weight and dry weight. These findings exhibited the potential of these five isolates as plant growth-promoting fungi to improve rice seedling growth and contribute to our understanding of the role of symbiotic fungi in sustainable rice crop production. Keywords: plant growth promoting fungi; seed treatment; plant-microbe interactions, symbiotic fungi
BACTERIAL CONTAMINATION TEST IN POWDER-FORMULATED Helicoverpa armige-ra NUCLEAR POLYHEDROSIS VIRUS (HaNPV1) SUBCULTURE Miranti, Mia; Kasmara, Hikmat; Fitriani, Nurullia; Melanie, Melanie; A'yun, Inas Qurrata; Syaputri, Yolani; Doni, Febri; Madihah, Madihah; Rahayuningsih, Sri Rejeki; Azizah, Nabilah Sekar; Hermawan, Wawan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2947

Abstract

The Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV1) is a subculture derived from the original HaNPV, and it has been cultivated in Spodoptera litura larvae as an alternative host. HaNPV1 was subsequently formulated using gypsum and talcum as carrier media. Following this formulation, a bacterial contamination test was conducted to assess the quality of the viral formulation.  The experiment was arranged in the randomized factorial block design (RFBD) with 2 replications. The viral formulations was stored for 16 weeks and the samples were taken every two weeks for contamination analysis. The data was then analyzed with the analysis of variance (ANOVA) and a post-hoc using Duncan’s Multiple Range test. The variable observed was the number of the bacterial colonies cultivated on the specific media i.e., Nutrient Agar (NA), Salmonella Shigella Agar (SSA) and Eosin Methilen Blue Agar (EMB). The results showed that the bacterial contaminants was detected from 0 to 12 weeks of storage time. However, the highest contamination was found in viral formulation after 8 weeks of storage time and the highest bacterial contaminations were recorded from all viral formulation tested in NA. The results indicated that the bacterial contamination were found around 1.45 × 109 cfu/gram and 1.97 × 109 cfu/gram in gypsum and talcum formulations, respectively. On SSA and EMB media, the bacteria contaminants from all formulation found in 8 weeks of storage time, but Salmonella, Shigella, or Escherichia coli (aspathogenic bacteria) were not found. After 12 weeks storage time, there was no indication of  contamination found in all media. Furthermore, Bacillus species was found as a most dominant contaminant in all samples. In conclusion, although the viral formulations using gypsum and talc were not contaminated by pathogenic bacteria such Salmonella, Shigella, or E. coli. Nevetherless, the viral formulation was still easily contaminated by other non-pathogenic bacterial species. Thus, a more standardized and stricted strategy needs to be developed for a better viral formulation product.
Co-Authors A'yun, Inas Qurrata Akbari, Sulistya Ika Almira Salma Nabilah Amalia, Eri Atasya Yasmine Fakhira Azizah, Nabilah Sekar Azwir Anhar Dwi Astuti Aprilia Dwi Hilda Putri Hikmat Kasmara Kalasuba, Karina Linda Advinda Madihah Madihah, Madihah Melanie Melanie, Melanie Mia Miranti Mia Miranti Rustama Nia Rossiana Nurullia Fitriani Prismantoro, Dedat Rizky Riscahya Pratama Syamsuri Sri Rejeki Rahayuningsih Sulistya Ika Akbari Syaputri, Yolani Wawan Hermawan