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Journal : JURNAL ATOMIK

PENENTUAN VARIASI KOMPOSISI DAN pH OPTIMUM ADSORBEN KITOSAN-Fe3O4 BERDASARKAN PERSEN PENYERAPAN ION Pb2+ Lianasari, Ika Yekti; Koesnarpadi, Soerja; Pratiwi, Djihan Ryn; Munandar, Aris
JURNAL ATOMIK Vol 8 No 1 (2023)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Previously, the synthesis of Chitosan-Fe3O4 adsorbent from magnetite (Fe3O4) made from ferrous ammonium sulfate (III) and ferrous ammonium sulfate (II) in a ratio of 3:1 was carried out. The adsorbent is adjusted with the ratio of magnetite and chitosan composition 1:1; 1:2 and 2:1 with the help of glutaraldehyde as a crosslinker. The results showed that the ratio of the ideal composition is 1:1 with % absorption that is 94.97% in 24 hour, and the optimum pH is obtained at pH 5 with % absorption of 92.18% for Pb2+ ions.
UJI FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN (METODE DPPH) DARI DAUN RAMBAI (Baccaurea motleyana Mull. Arg.) Ami, Suyatmi; Saleh, Chairul; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 4 No 2 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

The research about phytochemical test and antioxidant activity test on rambai leaves extract (Baccaurea motleyana Müll.Arg.) using DPPH radical immersion method had been done. This research aims to know kinds of secondary metabolite compounds in rambai leaves extract and level of antioxidant activity in rambai leaf. The result of this research showed that the secondary metabolite in rambai leaf were alkaloids, phenolics, flavonoids, saponins, steroids and triterpenoids. IC50 values obtained in each crude extract, n-hexane fraction, ethyl acetate fraction and residual ethanol fraction were 13.27 ppm, 22.81 ppm, 19.22 ppm and 13.33 ppm respectively. The highest level of antioxidant activity was in crude extracts.
SCREENING OF LIPASE FROM WATER BACTERIA IN BENANGA LEMPAKE DAM, NORTH SAMARINDA AND ITS POTENTIAL AS A DETERGENT ADDITIVE Vera, Hendrica Mini; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 8 No 2 (2023)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Lipase is an enzyme that can hydrolyze triglycerol into glycerol and free fatty acids. The aim of this research is to obtain lipase-producing bacteria and determine the potential of lipase from water bacteria in the water of the Benanga Lempake Dam, North Samarinda for biodetergent applications through stability test of lipase activity against several commercial detergents. Screening of lipase-producing bacteria was carried out on nutrient agar media containing olive oil and Rhodamine B. The results showed that seven isolates were capable of producing lipase. Based on the stability test of lipase on detergent, it can be seen that the activity of lipase decreased by more than 70% at the addition of 1% detergent.
AKTIVITAS AMILASE PENDEGRADASI PATI MENTAH DARI BAKTERI TANAH TEMPAT PEMBUANGAN AKHIR BUKIT PINANG Putri, Amanda Aulia; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 9 No 2 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i2.1408

Abstract

Raw starch-degrading amylase (RSDA) is a hydrolytic enzyme that can degrade raw starch into glucose. This study was conducted to obtain raw starch-degrading amylase-producing bacteria from the soil of the Bukit Pinang Landfill, Samarinda City. Isolation of bacteria using the spread plate method. Screening of RSDA-producing bacteria using nutrient media to contain raw starch. Isolation of bacteria obtained 10 single colonies of bacteria. The isolated bacteria were selected for their ability to produce RSDA. All single colonies of bacteria could produce RSDA and their activity was tested quantitatively using the 3,5 Dinitrosalicylic Acid (DNS) method. Bacteria with the TPA 1 colony code had the highest activity of 3.837 U/mL.
PHYTOCHEMICAL AND TOXICITY TEST OF MERUNG EXTRACTS (Coptosapelta tomentosa (Blume) Karolina, Anita; Pratiwi, Djihan Ryn; Akkas, Erwin
JURNAL ATOMIK Vol 3 No 2 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

This study was conducted to determine the content of secondary metabolites and the toxicity of extracts of Merung various parts (Coptosapelta tomentosa (Blume). The research methods included maceration, phytochemical tests and toxicity tests using the BSLT method. Based on the results of the phytochemical test showed all extracts of Merung parts contained steroids, flavonoids, and phenolics. Saponin is only found in leaf extract. Toxicity tests using the BSLT method showed that stem and root extracts were toxic with LC50 values of 2004.06 and 173.09 ppm, respectively.
PHYTOCHEMICAL TEST AND ANTIBACTERIAL ACTIVITY OF METHANOL EXTRACT OF Melicope lunu-ankenda (Gaertn.) T.G. Hartley STEM AGAINST Salmonella typhi AND Staphylococcus aureus Hermawan, Hermawan; Wirawan, Teguh; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 6 No 1 (2021)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Phytochemical test and antibacterial activity of methanol extract of Melicope lunu-ankenda (Gaertn.) T.G. Hartley have been done. The extraction of Melicope lunu-ankenda (Gaertn.) T.G. Hartley stem sample was carried out by the maceration method using methanol as a solvent. An antibacterial activity test was carried out using the agar diffusion method. Phytochemical tests results showed that methanol extract of Melicope lunu-ankenda (Gaertn.) T.G. Hartley stem has secondary metabolites including alkaloids, steroids, triterpenoids, and quinones. The antibacterial activity test results obtained MIC values for Salmonella typhi bacteria of 1.25% and for Staphylococcus aureus bacteria of 0.625%. Methanol extract of Melicope lunu-ankenda (Gaertn.) T.G. Hartley stem has broad-spectrum antibacterial activity.
SCREENING AND PHYTOCHEMICALS TEST OF EXTRACT ENDHOPHYTES BACTERIA FROM STEM OF PACING (Costus sp.) Aristina, Reza Fauziah; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 4 No 1 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Pacing plants (Costus sp.) Potentially as medicinal plants, but there is no research on the endophytic bacteria they contain. This research was conducted to filter endophytic bacteria from Pacing stems (Costus sp.) And to find out the secondary metabolites contained by each endophytic bacteria. Endophytic bacteria obtained from Pacing Stems (Costus sp.) Totaled 17 colonies. Crude extracts from all bacterial colonies containing alkaloids, 15 crude extracts containing saponins. Steroids are only contained by 3 types of bacterial extracts and triterpenoids are only contained by 2 types of bacterial extract.
SKRINING FITOKIMIA DAN BIOAKTIVITAS EKSTRAK AKAR Uncaria nervosa Elmer (BAJAKAH) Maulina, Sheli; Pratiwi, Djihan Ryn; Erwin, Erwin
JURNAL ATOMIK Vol 4 No 2 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

One type of Bajakah that is believed by local people especially in the area of Muara Badak, KutaiKertanegara Regency, has the efficacy of anticancer drugs, is Uncaria nervosa Elmer. The purposes of this studywere to determine the content of secondary metabolites and the toxicity of the extracts of the bark and root of thewood of Bajakah (U. nervosa). From the results of phytochemical tests showed that both bark and root woodcontain secondary metabolites of alkaloids, flavonoids, terpenoids, and phenolics. Moreover, based on the result ofthe mortality test for Artemia salina shrimp larvae, it showed that both the bark and wood of the roots are verytoxic with LC50 values of 1.76 and 2.66 ppm, respectively. Both of these extracts are potentially cytotoxic to cancercells.
THE PHYTOCHEMICAL TEST AND ANTIOXIDANT ACTIVITY (DPPH METHOD) FROM LEAVES OF BIRIBA (Rollinia mucosa (Jacq.) Baill.) Sumarni, Feni; Saleh, Chairul; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 4 No 1 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Phytochemical test and antioxidant activity test of biriba leaves (Rollinia mucosa (Jacq.) Baill.) has been done. Extraction from biriba leaves was carried out using several solvents, namely ethanol, n-hexane and ethyl acetate. From phytochemical test results from leaves of biriba (R. mucosa) containing secondary metabolites namely alkaloids, phenolics, flavonoids and steroids. Based on the results of the antioxidant test using DPPH (1,1-diphenyl-2-picrylhidrazyl) method showed that the crude extract, n-hexane fraction, ethyl acetate fraction and residual ethanol fraction had IC50 values of 159.72; 119.62; 40.12 and 38.35 ppm, respectively. From the results of the antioxidant test it is known that the ethanol fraction of the remaining biriba leaves has the strongest antioxidant activity with IC50 values of 38.35 ppm.
UJI FITOKIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK METANOL AKAR MERUNG (Coptosapelta tomentosa) Dewi, Dynda Rosiana; Marliana, Eva; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 4 No 2 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Phytochemical tests and antibacterial activity of the roots of Merung plants (Coptosapelta tomentosa) have been done. This research aims to find out the content of secondary metabolites contained in the sample using the Thin Layer Chromatography method and to determine antibacterial activity by paper disc method from methanol extract Merung root (Coptosapelta tomemtosa). Based on results of phytochemical screening, it was known that methanol extract contained alkaloid, polyphenol, flavonoid and terpenoid/steroid. Methanol extract antibacterial activity against Propionibacterium acnes KCCM 11898 range of MIC of 0-1%. Methanol extract showed antibacterial activity against Escherichia coli bacteria ATCC 25922 range of MIC of 0-1%. From the results of the antibacterial activity test it can be concluded that the methanol extract have antibacterial activity against Propionibacterium acne KCCM 11898 and Escherichia coli ATCC 25922.