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Journal : PROSIDING SEMINAR KIMIA

SKRINING FITOKIMIA DAN BIOAKTIVITAS RHODOMYRTUS TOMENTOSA (AITON) HASSK) (KARAMUNTING) Erwin, Erwin; Ilham, Muhammad Hafid; Pratiwi, Djihan Ryn; Alimuddin, Alimuddin
PROSIDING SEMINAR KIMIA Vol 2 No 2 (2024): Prosiding SNKT III 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Rhodomyrtus tomentosa (Aiton) Hassk) or known as Karamunting, is one of the medicinal plants. This medicinal plant is used by the community as a medicine for colic, fever, dysentery, sepsis, to treat shortness of breath or pain, tuberculosis, abscesses, bleeding and gynecopathy. In this study, phytochemical and bioactivity tests will be carried out on Rhodomyrtus tomentosa leaves, stem and bark extracts. Research methods include maceration of the sample with methanol, separation of the filtrate solvent obtained from the extract using a rotary evaporator, determination of chemical content using a phytochemical test, and determination of bioactivity using the brine shrimp lethality test (BSLT) method. Based on the results of phytochemical tests, it was found that the leaf extract contained alkaloids, steroids, phenolics, flavonoids, saponins and quinones. Stem extract contains alkaloids, phenolics, flavonoids, saponins and quinones. and stem bark extract contains triterpenoids, phenolics, saponins and quinones. Meanwhile, the results of the bioactivity test showed that only the leaf extract was toxic to Artemia salina shrimp with an LC50 of 582.10 ppm, while the leaf and stem bark extracts were inactive with an LC50 value above 1000 ppm. Keywords: Karamunting, BSLT test, phytochemical test, and Artemia Salina L
PENGARUH ION LOGAM TERHADAP AKTIVITAS LIPASE DARI BAKTERI AIR BEKAS GALIAN TAMBANG DI SAMARINDA Haeruddin, Haeruddin; Astuti, Winni; Pratiwi, Djihan Ryn
PROSIDING SEMINAR KIMIA Vol 2 No 1 (2023): Prosiding SNK 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Addition of metal ions on lipase activity has been done. The aim of this research is to find out the effect of metal ions on lipase activity from water bacterial post mining in Samarinda. Lipase activity was determined based on the Titrimetric method with olive oil as a substrate. The result showed lipase activity from water bacterial post mining without the addition of metal ions is 1,0 U/mL and with the addition of metal ions Mg2+, K+, Fe3+, Ca2+, dan Ba2+their respective lipase activities is 0,6 U/mL, 0,7 U/mL, 1,9 U/mL, 1,4 U/mL dan 0,8 U/mL. The conclusion that can be drawn that is metal ions K+, Mg2+ and Ba2+ inhibit lipase activity with a relative activity each at 70; 60 dan 80% while Ca2+ increase lipase activity with a relative activity at 140% and Fe3+ causes denatured lipase with a relative activity at 190%. Keywords: Bacteria, Lipase, Metal Affect