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INHIBITORY POWER TEST OF N-HEXANE EXTRACT OF MALAKA LEAVES (Phyllanthus emblica) ON GROWTH Microsporum canisIN-VITRO Miranda, Lola Almira; Asmilia, Nuzul; Fakhrurrazi, Fakhrurrazi; Rusli, Rusli; Amiruddin, Amiruddin; Jalaluddin, M.
The International Journal of Tropical Veterinary and Biomedical Research Vol 10, No 1 (2025): Vol 10 (1) May 2025
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v10i1.48192

Abstract

Background and Aim: Malacca leaves are one of the medical plants that can be used in traditional medicine. Malacca leaves contain several active compounds that can be effective in inhibiting fungal growth. This study aims to investigate the inhibition of n-hexane Malacca leaves extract on Microsporum canis growth in vitro.Materials and Methods: The method used in this study is Kirby-Bauer disc diffusion with 5 treatments, namely P1 (25% Malacca leaves n-hexane extract), P2 (50% Malacca leaves n-hexane extract), P3 (75% Malacca leaves n-hexane extract), P4 (control (+): ketoconazole) and P5 (control (-): carboxymethylcellulose (CMC) 1%) with 3 replications. The clear zone formed around the disc was measured using a caliper.Results : The results showed that there was no clear zone formed around the disks of the n-hexane extract of Malacca leaves. It can be concluded that the n-hexane extract of Malacca leaves could not inhibit the growth of Microsporum canis.Conclusion: The findings of this study indicate that the n-hexane extract of Malacca leaves, at concentrations of 25%, 50%, and 75%, does not exhibit antifungal activity against Microsporum canis in vitro, as evidenced by the absence of inhibition zones. Therefore, n-hexane may not be an effective solvent for extracting antifungal compounds from Malacca leaves against this fungal species.
STUDI ANATOMIS DAN HISTOLOGIS PANKREAS BIAWAK AIR (Varanus salvator) (Anatomical and Histological Study of the Pancreas of Water Monitor Lizard (Varanus salvator)) Hamny, Hamny; Iqbal, Muhammad; Wahyuni, Sri; Sabri, Mustafa; Jalaluddin, M.; Rinidar, Rinidar
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5047

Abstract

The aim of this research was to determine the anatomy and histology of pancreas of water monitor lizard (Varanus salvator). Two pancreas were used in this research (a male and a female). Water monitor lizard was euthanized using chloroform and subsequently necropsied to collect the pancreas. The pancreas was washed in 0.9 % NaCl solution. Anatomical observations were carried out including location, shape, and color of pancreas, also the length and weight measurements. Then, pancreas was fixed with 4% paraformaldehyde for 7 days to proceed to histological preparation and hematoxylin eosin (HE) staining. The results of the anatomical observations showed that pancreas of water monitor lizard have two lobes, dorsal lobe (splenic lobe) and ventral lobe (duodenal lobe). Dorsal lobe which was in oval form adhered with the spleen, and ventral lobe was located in the cranial of intestines with the form of long spherical. Both of pancreas lobes were white-yellow color with the length of 1.450.64 cm (dorsal lobe) and 7.000.42 cm (ventral lobe). Weight of dorsal lobe was 0.150.01 g and ventral lobe was 6.352.30 g. The histological observations found the acinar cells, ducts, and blood vessels in both of pancreas lobes. Additionally, the similar structure of Langerhans islet only found at the ventral lobe of pancreas. In conclusion, pancreas of water monitor lizard consists of two lobes which located differently. Ventral lobe is larger than the dorsal lobe. Both lobes have similar histological structure except the Langerhans islets are only appeared in the ventral lobe.
Gambaran Makroanatomi dan Mikroanatomi Bursa Fabrisius Ayam Pedaging yang Diberi Pakan Tepung Larva (Maggot) Black Soldier Fly Wardhani, Lailia Dwi Kusuma; Jalaluddin, M.; Firdausi, Nida Khofiya; Sofyan, Hamny; Masyitha, Dian; Wahyuni, Sri; Sabri, Mustafa; Rahmawati, Indra; Nussa, Olan Rahayu Puji Astuti; Ritonga, Mudhita Zikkrullah
Jurnal Veteriner Vol. 27 No. 1 (2026)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tepung larva (maggot) Black Soldier Fly (BSF) terhadap makroanatomi dan mikroanatomi serta interaksi organ bursa Fabrisius ayam pedaging umur berbeda. Penelitian ini menggunakan rancangan acak lengkap dengan dua faktor yaitu perlakuan maggot BSF dan umur ayam. Sampel penelitian menggunakan 24 ekor ayam pedaging. Faktor perlakuan maggot BSF sebanyak empat kelompok yang terdiri atas P0 (Pakan komersil 100%), P1 (Pakan komersil 90% + tepung maggot BSF 10%), P2 (Pakan komersil 80% + tepung maggot BSF 20%), dan P3 (Pakan komersil 70% + tepung maggot BSF 30%). Faktor umur ayam terbagi dua yaitu umur 14 hari dan 21 hari. Perlakuan penelitian selama 14 hari. Sampel ayam dinekropsi untuk diambil organ bursa Fabrisius. Sampel organ dibuat preparat histologi dan diwarnai dengan Hematoksilin-Eosin. Data warna dan bentuk bursa Fabrisius dianalisis secara deskriptif, sedangka data hasil pengukuran histologinya dianalisis menggunakan uji sidik ragam dua arah) dan uji Tukey. Hasil penelitian ini menunjukan bahwa pemberian pakan tepung maggot BSF dan umur ayam memberikan pengaruh pada gambaran makroanatomi bursa Fabrisius pada hasil tertinggi yaitu panjang (2,50 ± 0,07 cm), lebar (2,20 ± 0,10 cm), bobot (1,89 ± 0,511 g). Pada pengukuran mikroanatomi, bursa Fabrisius pemberian pakan tepung maggot BSF menunjukkan pengaruh pada panjang folikel (0,85 ± 0,02 mm), lebar folikel (0,44 ± 0,02 mm), serta tinggi plicae (4,84 ± 0,35 mm) dipengaruhi oleh perbedaan umur.. Interaksi antara faktor perlakuan dan umur ayam menunjukkan interaksi terhadap panjang, lebar dan bobot bursa, panjang dan lebar folikel bursa Fabrisius. Simpulan dari studi adalah pemberian tepung maggot BSF sebagai pakan ayam pedaging memberikan pengaruh  pada organ bursa Fabrisius dengan perlakuan terbaik pada perlakuan P3 atau ransum dengan 30% tepung maggot BSF dan 70% pakan komersial.