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Pengukuran suhu, pH dan kadar substrat terhadap aktivitas enzim Galactose-1-Phosphate Uridyltransferase Sisca Sisca; Edy Parwanto; Monica Dwi Hartanti; David Tjahyadi
Jurnal Indonesia Sosial Sains Vol. 5 No. 07 (2024): Jurnal Indonesia Sosial Sains
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jiss.v5i07.1177

Galactose-1-phosphate uridyltransferase (GALT) is an important enzyme involved in galactose metabolism. Understanding the factors influencing GALT activity is critical to elucidate its physiological role and potential therapeutic implications in galactosemia. In developing new drugs, chicken intestine/liver powder can be used as an enzyme source, GALT, to treat galactosemia. Therefore, it is necessary to research the characterization of the GALT enzyme in chicken intestine and liver powder. In this study, we investigated the influence of temperature, pH, and substrate level on GALT enzyme activity using an experimental approach in vitro. The optimum pH extraction results show that the optimum pH for the extraction of the chicken intestine and liver GALT is pH 7, with activity values of 0.47 units/mL and 0.3953 units/mL, respectively. The optimum temperature for the extraction of chicken intestine and liver GALT is 37℃ with substrate hydrolysis capabilities of 0.48 U/mL and 0.57 U/mL, respectively. Meanwhile, the optimum substrate content is 400x. These insights provide a valuable foundation for further research aimed at comprehensively understanding GALT function, developing targeted interventions for disorders of galactose metabolism, and possible application in the development of new drugs for galactosemia

Exploring the Relationship Between Sod1, 2 And 3 Gene Polymorphisms With Post-Covid19 Symptoms Jihan Samira Thabit; Sisca Sisca; Monica Dwi Hartanti; Noviani Prasetyaningsih; Alvionita Kogoya; Arleen Devita; Isa Bela; Ida Effendi
Jurnal Indonesia Sosial Sains Vol. 5 No. 04 (2024): Jurnal Indonesia Sosial Sains
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jiss.v5i04.1097

The coronavirus disease 19 (COVID-19) has become a challenge for the media world. Even though they have been declared cured, some Covid-19 survivors still have health complaints. Abnormal symptoms, signs, or clinical parameters that persist two weeks or more after the onset of COVID-19 and do not return to their initial healthy state are potentially considered long-term effects of the disease. Although such changes are primarily reported in people with severe and critical illness, lasting effects also occur in individuals with mild infections that do not require hospitalization. This study aims to explore the role of antioxidants on the pathogenesis of Covid19 and its relationship with SOD1, 2 and 3 genomic variations. Getting alternative biomarkers for long covid19 detection. Research Method: The research sample is blood and questionnaires that will be taken from respondents affected by Covid19 a maximum of 6 months before data collection is held. Furthermore, DNA isolation, DNA amplification, cutting with restriction enzymes, and DNA band documentation with gel electrophoresis will be carried out. Symptoms related to systemic are the most common symptoms found in respondents with Long Covid19. A total of 8 respondents had systemic related symptoms, namely weakness, lethargy and sweating, while skin-related complaints were found in three respondents and one respondent had lung-related complaints. The average SOD activity value of respondents was 2.19 U/ml. In this study, more symptoms of long COVID-19 were found associated with systemic and cardiovascular symptoms. The complaints were not differentiated by the sex of the respondents. The output draft will be processed immediately after getting other results.

Pengukuran suhu, pH dan kadar substrat terhadap aktivitas enzim Galactose-1-Phosphate Uridyltransferase Sisca Sisca; Edy Parwanto; Monica Dwi Hartanti; David Tjahyadi
Jurnal Indonesia Sosial Sains Vol. 5 No. 07 (2024): Jurnal Indonesia Sosial Sains
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jiss.v5i07.1177

Galactose-1-phosphate uridyltransferase (GALT) is an important enzyme involved in galactose metabolism. Understanding the factors influencing GALT activity is critical to elucidate its physiological role and potential therapeutic implications in galactosemia. In developing new drugs, chicken intestine/liver powder can be used as an enzyme source, GALT, to treat galactosemia. Therefore, it is necessary to research the characterization of the GALT enzyme in chicken intestine and liver powder. In this study, we investigated the influence of temperature, pH, and substrate level on GALT enzyme activity using an experimental approach in vitro. The optimum pH extraction results show that the optimum pH for the extraction of the chicken intestine and liver GALT is pH 7, with activity values of 0.47 units/mL and 0.3953 units/mL, respectively. The optimum temperature for the extraction of chicken intestine and liver GALT is 37℃ with substrate hydrolysis capabilities of 0.48 U/mL and 0.57 U/mL, respectively. Meanwhile, the optimum substrate content is 400x. These insights provide a valuable foundation for further research aimed at comprehensively understanding GALT function, developing targeted interventions for disorders of galactose metabolism, and possible application in the development of new drugs for galactosemia

Pelayanan Kesehatan Terhadap Lansia Dalam Meningkatkan Kualitas Hidup Di Kelurahan Meruya Selatan Jakarta Sisca; Monica Dwi Hartanti; Orliando Roeslan; David; Yani Kurniawan; ML Edy Parwanto; Joey Joshua Vidova Tjahyadi
AMMA : Jurnal Pengabdian Masyarakat Vol. 3 No. 2 : Maret (2024): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

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Elderly people are part of the life process that cannot be avoided and will be experienced by every human being. Objective: to help the elderly community in the area of the Lukas Church of Maria Kusuma Karmel Parish, West Jakarta, Meruya Selatan Subdistrict to improve their quality of life, especially in the physical domain, through counseling activities, health services and treatment so that it is hoped that it can improve the quality of life of the elderly. Method: Health services were provided to 60-70 elderly people in South Meruya Village. The service team consists of 6 lecturers and 3 students. Blood pressure, height and weight, glucose, uric acid and cholesterol were measured. Results: High blood pressure was checked for around 37% of elderly people, 33% for cholesterol, 10% for glucose and 57% for uric acid. Conclusion: Regular health checks are an important part of comprehensive prevention and management of chronic diseases. Family and local community support can help elderly residents undergo treatment to improve their quality of life.

Penyuluhan Dan Konseling Dalam Meningkatkan Kepedulian Sosial Lansia Di Kelurahan Meruya Selatan Jakarta Monica Dwi Hartanti; Sisca; Wita Anggraini; Dyani Pitra Velyani; Joey Joshua Vidova Tjahyadi
AMMA : Jurnal Pengabdian Masyarakat Vol. 3 No. 5 : Juni (2024): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

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The increase in the elderly population in Meruya Selatan Subdistrict, Jakarta, demands efforts to increase their social awareness and welfare. This research aims to evaluate the effectiveness of outreach and counseling in improving the social care of the elderly in the area. The methods used include data collection on the elderly, conducting workshops, individual and group counseling sessions, as well as developing social and recreational activities.The results showed an increase in the social participation of elderly people, a decrease in loneliness levels, and an increase in psychological well-being. Seniors who take part in the program demonstrate active involvement in community activities and have a better understanding of the importance of physical and mental health. Challenges faced include limited resources and difficulty mobilizing participants. This program has succeeded in improving the quality of life of the elderly through a comprehensive and collaborative approach. Recommendations for further development include increasing the capacity of the extension team and counselors, utilizing digital technology, and developing infrastructure that is elderly friendly. This research underscores the importance of ongoing and collaborative social support for the well-being of older adults.

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