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PROFIL KADAR MDA (MALONDIALDEHIDE) SEBAGAI PENANDA KERUSAKAN SELULER AKIBAT RADIKAL BEBAS PADA TIKUS YANG DIBERIKAN AIR BEROKSIGEN Zaetun, Siti; Dewi, Lale Budi Kusuma; Wadnya, Ida Bagus Rai; Srigede, Lalu
Jurnal Analis Medika Biosains (JAMBS) Vol 4, No 1 (2017): JURNAL ANALIS MEDIKA BIOSAINS
Publisher : Jurusan Analis Kesehatan, Poltekkes Kemenkes Mataram

Radikal bebas dapat menyebabkan stres oksidatif, karena ketidakseimbangan antara oksidan dan antioksidan yang berpotensi menyebabkan kerusakan sel. Radikal bebas dapat meningkatkan peroksidasi lipid, yang terurai menjadi malondialdehyde (MDA) dalam darah. MDA adalah penanda cacat seluler yang disebabkan oleh radikal bebas. Penelitian ini bertujuan untuk menentukan profil MDA (malondialdehyde) sebagai penanda cacat sel yang disebabkan oleh radikal bebas pada tikus yang diolah dengan air yang mengandung oksigen. Ini adalah penelitian eksperimental dengan 3 kelompok perawatan. Satu kelompok sebagai kontrol dan dua kelompok sebagai sampel yang diolah dengan air beroksigen selama 5 hari dengan volume tertentu. Data dianalisis dengan mengukur tingkat kontrol kelompok MDA dan sampel kelompok. Data diuji dengan Uji Kruskal Wallis dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa rata-rata kadar MDA pada kelompok yang diolah dengan air pentagonal, air heksagonal dan kelompok kontrol adalah 5,09 μM / L, 3,14 μM / L dan 3,06 μM / L. Analisis data menunjukkan tidak ada perbedaan yang signifikan dalam tingkat MDA pada kelompok perlakuan. Ini menyimpulkan bahwa tidak ada perbedaan tingkat MDA yang signifikan pada tikus yang diolah dengan air yang mengandung oksigen. Ini berarti tidak ada efek pada kerusakan seluler.

The Determination of Haemostasis Value in Relation to Potential Blood Leaf Leaves Filtrate (Excoecaria cochinchinensis L) as the External Medicine for Skin Wound of White Rat (Rattus norvegicus) Zaetun, Siti; Srigede, Lalu
Indonesian Journal of Medicine Vol 5, No 1 (2020)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.909 KB)

Background: Plants are currently used as medicine, i.e. medicinal plants. Medicinal plants conÂsist of many chemical compounds, especially bioactive substances. One of them is Sambang Darah (Excoecaria cochinchinensis). Sambang Darah have so many benefits which usually applied in vulnus combutio. This study aimed to determine value of haemostasis after applied Sambang Darah leaves in white rat skins (Rattus norvegicus).Subjects and Method: This was a true experimental study with post test only control group deÂsign. This study conducted at Mata-ram University, West Nusa Tenggara, Indo-nesia, from April to October 2017. A total sam-ple of 16 whiÂte rats was selected for this study. The dependent variable in this study was the value of haemostasis and the independent vari-able was filtrate of Sambang Darah leaves. The data were analyzed using Man Whitney test.Results: The average level of bleeding time was 3.76 minutes for the control group and 2.58 miÂnuÂtes for test group. The average level of clotting time were 2.20 minutes for control group and 1.36 miÂnutes test group. The number of platelet was 277,000/uL for the control group and 221,000/uL for test group. APTT level was25.06 second for the control group and there was no clot in test groÂup. PT level was 13.95 second for the control group and there was no clot in test group. TT levels was 18.2 second for the control group and there was no clot in test group. And the last, D Dimer level was 0.1 mg/l for the control group and there was no clot in test group. The statistical analysis shoÂwed p â‰¤0.001.Conclusion: Filtrat of sambang darah leaves can be applied as medicine for external wounds but it can not be applied in koagulasi test.Keywords: Haemostasis value, blood leaf filtrate (Excoecaria cochinchinensis L)Correspondence:Â Siti Zaetun.Program Study of Health Analyst, School of Health Polytechnic, Ministry of Health, Mataram. Jl. Prabu Rangkasari, Dasan Cermen, Sandubaya, Mataram, West Nusa Tenggara. Email: miraigan@yahoo.co.id. Mo-bile: 081803630063.Indonesian Journal of Medicine (2020), 5(1): 10-16https://doi.org/10.26911/theijmed.2020.05.01.02

Analysis of the Effect of Taking Anti-Tuberculosis Drugs (ATD) on Blood Glucose Levels and Urine Protein Levels in Tuberculosis Sufferers at the Dasan Tapen Community Health Center, West Lombok Wahyuni, Leni; Agrijanti, Agrijanti; Srigede, Lalu
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.354

Tuberculosis is a contagious infectious disease caused by the bacteria Mycobacterium tuberculosis or often called Acid-Fast Bacteria (AFC). Tuberculosis positive patients must undergo treatment by administering category 1 Anti-Tuberculosis Drugs (ATD) for approximately 6-8 months. Anti-Tuberculosis Drugs (ATD) are divided into two phases, namely the intensive phase and the advanced phase. Consuming Anti-Tuberculosis Drugs (ATD) for a long period of time can cause the drug to become toxic in the body, so that it can affect body organs such as the kidneys and pancreas. The aim of this study was to measure blood glucose levels, measure urine protein levels and analyze blood glucose levels and urine protein levels in tuberculosis sufferers who took Anti-Tuberculosis Drugs (ATD). Using analytical observational research methods with a cross sectional research design . Tuberculosis sufferers are checked for blood glucose and urine protein, first and second collection. The results of research on 24 tuberculosis patients who took Anti-Tuberculosis Drugs (ATD) at the Dasan Tapen Community Health Center, West Lombok showed that the blood glucose levels of tuberculosis patients who took intensive phase ATD, namely of the 14 patients, 9 patients experienced an increase. Meanwhile, in the continuation phase, of the 10 patients, there were 6 patients who experienced improvement. The urine protein levels of tuberculosis sufferers who consumed ATD in the intensive and follow-up phase of the first examination were positive (+1) in 1 sample while negative (-) in 23 samples. In the second examination, 2 samples were positive (+1), while 22 samples were negative (-). The conclusion of this study is that there is no relationship between the first and second examination of random blood glucose levels and random urine protein levels.

Perbandingan Jumlah Trombosit pada Pasien COVID-19 dengan Kadar Glukosa yang Berbeda Octavira, Imanta Alifia; Agrijanti, Agrijanti; Srigede, Lalu; Zaetun, Siti
Jurnal Kesehatan Andalas Vol. 11 No. 2 (2022): Online July 2022
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v11i2.2000

Hyperglycemia has increased arachidonic acid thromboxane which is an effective ingredient in platelet activation. Thrombocytopenia in COVID-19 patients occurs through a variety of mechanisms, such as uncontrolled production of cytokines destroying bone marrow progenitor cells, direct inhibition of hematopoiesis viral infection of the bone marrow, increase in autoantibodies and immune complexes that cause platelet destruction, and lung injury that causes platelet aggregation and consumption of platelets so that platelets in the circulation are reduced. Objectives: To analyzed the ratio of platelet counts in COVID-19-positive patients who had different blood glucose levels. Methods: This research used secondary data, with types of analytical observational research as well as comparative study approaches. Statistical tests were conducted using an independent samples t-test with a confidence level of 95%. Results: The average platelet count was normal, but a low platelet count of 3.30% was obtained with normal blood glucose levels and a high platelet count of 2.20% with normal blood glucose levels, with a significance value of platelet count with normal blood glucose levels of 0.790 and platelet counts with blood glucose levels above normal which is 0.791 with a confidence level of 95%. Conclusion: There was no significant difference between the platelet count in COVID-19 patients with normal and above normal blood glucose levelsKeywords: blood glucose, platelets, COVID-19

Cross Test of ICT-TB and Genexpert Examination in Suspected Cases of M. tuberculosis Infection at Bima Regency Regional Hospital Wiadnya, Ida Bagus Rai; Sani, Syahrul; Srigede, Lalu
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 1 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i1.426

GeneXpert is able to detect specimens that are positive for M. tuberculosis in a shorter time and with high sensitivity and specificity. ICT-TB is used to detect the presence of antibodies produced by M. tuberculosis infection in the body. This response requires time to form. However, actually ICT-TB is an easier method compared to Genexpert. So this research was carried out to compare the results of the two methods. Research Objective: To determine the results of cross-testing of ICT-TB and Genexpert examinations on cases of suspected M. tubeculosis infection at the Bima Regency Hospital. Research Method: This is an analytical observational study with a cross-sectional design. The samples used in this research were 52 samples and the data were analyzed descriptively. Research Results: The distribution of suspected tuberculosis based on gender is 54% male and 46% female. Based on age group, Toddlers 13.5%, Children 11.5%, Teenagers 9.6%, Adults 44.2% and Elderly 21.2%. ICT-TB examination results are negative. Examination with Genexpert on the same patient was negative. Conclusion: From the results of research using ICT-TB and GeneXpert in patients with suspected M. tuberculosis infection, negative results were obtained.

Correlation of Blood Creatinine Levels with The Result of Urine Sediment Analysis in Chronic Kidney Failure Patient Zaetun, Siti; Rohmi, Rohmi; Oktaviani, Andi Dwi; Srigede, Lalu
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.339

Chronic Kidney Failure is a condition caused by a gradual and chronic decline in kidney function. This situation can affect to creatinine levels. Creatinine is the result of endogenous metabolism that is useful for assessing glomerular function, in the event of renal dysfunction then the filtration ability of creatinine will be reduced and serum creatinine will increase. Urine sediment is produced from the results of urine concentration consisting of calcium salts. This concentrated urine comes from the kidneys with a variety of causes, one of which is chronic kidney failure, flows through the ureter to the bladder and continues to the urethra until the urethral. Objective: To find out the correlation of blood creatinine levels with the results of urine sediment analysis in patients with chronic kidney failure. Method: This research is observational analytical with a cross sectional approach to determine the correlation between independent and dependent variables. The sample is taken purposive sampling by selecting a sample based on certain criteria. The collected data is then analyzed using Spearman statistical tests. Results: Examination of creatinine levels in chronic kidney failure patients obtained 6.67 mg/dl. The average urine sediment found erythrocytes as many 2-3/HPF to many/HPF. Conclusion: There is a correlation of blood creatinine levels with the results of urine sediment analysis in patients with chronic kidney failure.

Utilization Of Kuku Pancar (Lawsonia Inermis Lin) Leaves as a Substitute For Eosin Dye in The Examination of Soil Transmitted Helminth (STH) Eggs Yusari, Lala Deswinta; Srigede, Lalu; Manu, Thomas Tandi; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.356

Nail surfacing leaves (Lawsonia inermis lin) are natural materials commonly used as dyes, because of the content of chemical compounds in them such as lawsone, flavonoids, tannins and anthocyanins, therefore nail surfacing leaves can be used as an alternative dye to replace 2% Eosin in STH worm egg examination, because it has the same properties as 2% Eosin and can produce an orange yellow color after the extraction process. The research objective is to determine the results of nail surfacing leaves (Lawsonia inermis lin) can be used as an alternative substance to replace Eosin in the examination of STH (soil transmitted helminth) worm eggs. The methods of this research is using is experimental, where this study looks at the clarity of soil transmitted helminth (STH) worm eggs on preparations, using coloring from the results of soaking the leaves of the nail surf as a substitute for Eosin. The results of the research is that the coloring results obtained in each variation of immersion time are good. The conclusion of this research is that the staining of worm eggs using the results of immersion of nail surfacing leaves for 12 hours, 24 hours, 36 hours, and 48 hours can be used as an alternative dye in the examination of STH worm eggs.

Description of Uric Acid Levels and Blood Pressure In Pulmonary Tuberculosis Patients Consuming Anti Tuberculosis Drug (ATD) in the Working Area of Sakra Public Health Center Wahyudi, Nizar; Getas, I Wayan; Wiadnya, Ida Bagus; Srigede, Lalu
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.302

Background: Anti-tuberculosis (ATD) used is the standard blend of INH, PAS and streptomycin for one to two years. The amino salylic (pas) acids are then replaced with pirazinacids. Pirazinbacteria is a drug that can cause increased levels of uric acid in the body (hyacinth). Hiperurisemia is the state of concentration of the veins in the plasma or the serum has exceeded the limit of the monosodium ligament of 7.0 milligrams /dl. Hyperuricemia is also cited as an important risk factor for hypertension and other cardiovascular diseases. Research purposes: For details of uric acid and blood pressure on lung tuberculosis patients who use antituberculosis (oats) in the region of the sakra hospital. Research methods: The kind of research used I s a descriptive observation study with a sectional design, a study in which dependent and independent variables are done and measured simultaneously. The sample retrieval technique on this study USES a total sample using the criteria the researchers have chosen in selecting a sample. Research: Average uric acid and blood pressure in lung tuberculosis patients who consume single-month intensive drugs (ATD) are 7,8 mg/dl and 121/75 mmHg. Average levels of uric acid and blood pressure in lung tuberculosis patients who use 2 months of intensive phase (ATD) are 9,1 mg/dl and 129/78 mmHg. Conclusion: There are high levels of uric acid and blood pressure in those with lung TB who take anti-tuberculosis drugs (ATD).

Hematocrite Values With High Measurement Of Eritrosit After Centrifugation On Serum Making Jiwintarum, Yunan; Srigede, Lalu; Asyhaer, Rifki Khalidi
Jurnal Analis Medika Biosains (JAMBS) Vol 7, No 2 (2020): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v7i2.193

Tujuan Penelitian ini untuk mengetahui apakah nilai hematokrit darah dapat diukur dengan pengukuran tinggi endapan eritrosit setelah disentrifugasi pada pembuatan serum. Jenis penelitian ini adalah Quasi Eksperiment dengan rancangan Posttest Only Control Group Design. Banyaknya perlakuan ada tiga, yaitu pembuatan serum dengan sentrifugasi kecepatan 3000 rpm selama 5 menit, 10 menit, dan 15 menit. Data yang dikumpulkan berupa nilai hematokrit dengan sentrifugasi pada pembuatan serum dibandingkan dengan nilai hematokrit dengan metode mikrohematokrit. Data dianalisis menggunakan uji Kruskal-Wallis. Hasil menunjukkan bahwa rerata nilai hematokrit dengan metode mikrohematokrit adalah 46%, sedangkan rerata nilai hematokrit dengan pengukuran tinggi endapan eritrosit pada pembuatan serum setelah disentrifugasi selama 5 menit, 10 menit, dan 15 menit adalah 58,5%, 57,6%, dan 48,1%. Uji statistik menunjukkan adanya perbedaan yang signifikan hasil pemeriksaan metode mikrohematokrit dan pemeriksaan dengan sentrifugasi 5 menit maupun 10 menit. Sedangkan antara metode mikrohematokrit dan pemeriksaan dengan sentrifugasi 15 menit tidak ada perbedaan yang signifikan. Kesimpulan : Pengukuran tinggi endapan eritrosit setelah disentrifugasi selama 15 menit pada pembuatan serum dapat digunakan untuk pemeriksaan hematokrit.

Effect of Aloe vera Gel Formulation and Jatropha multifida L Leaf on the Growth of Staphylococcus aureus Bacteria Dektra, I Made Putra Pramaditya; Pauzi, Iswari; Srigede, Lalu; Jiwintarum, Yunan
Hydrogen: Jurnal Kependidikan Kimia Vol. 13 No. 4 (2025): August 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i4.17104

Staphylococcus aureus bacteria is one of the agents of health problems that require special attention. Inappropriate use of antibiotics can cause resistance, so a more effective and safe alternative treatment is needed, one of which is by giving aloe vera gel formulations and tintir castor leaves that can help overcome bacterial infections, such as Staphylococcus aureus. Knowing the effect of aloe vera gel formulation (Aloe vera) and tintir castor leaf (Jatropha multifida) on the growth of Staphylococcus aureus bacteria. The study was pre-experimental with a cross sectional design. Gel formulations were made with various comparisons between aloe vera gel and tintir castor leaf, then tested against Staphylococcus aureus bacteria by observing the inhibition zone as an indicator of antibacterial activity. The results of testing the Aloe vera gel formulation and castor leaf filtrate showed that there was no inhibition zone in each of the tested formulation ratios. In the testing using Aloe vera gel formulations and Jatropha multifida leaf extract, no inhibition zones were observed in the 1:1 and 1:3 ratios, but an inhibition zone was present in the 3:1 ratio, measuring 4–6 mm with an average inhibition zone of 5 mm. This result can be considered sensitive but at a low level for inhibiting bacterial growth. There is an effect of Aloe vera gel and jarak tintir leaf formulation on the growth of Staphylococcus aureus bacteria.

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