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All Journal Jurnal Analis Medika Biosains (JAMBS) JPP Jurnal Kesehatan Poltekkes Palembang Jurnal Laboratorium Khatulistiwa Journal of Indonesia Laboratory Students (JILTS) Jurnal Indonesia Mengabdi
Manu, Thomas Tandi
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Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Gambaran Jumlah Leukosit Pada Penderita Suspek So TB Paru di Rumah Sakit Patuh Patut Patju Reginata, Ni Luh Vira; Rohmi, Rohmi; Manu, Thomas Tandi; Khusuma, Ari
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1453

Abstract

Leukocyte examination is carried out to describe disease events or processes in the body, aspecially infectious diseases such as tuberculosis bacteria can attack immune system cells, including leukocytes. Bacteria can survive and reproduce in leukocytes. Pulmonary TB sufferers before treatment saw an increases in leukocyte levels, indicating the formation of many leukocytes to fight bacteria in the phatocytosis process. On of the laboratory tests used to support the diagnosis of tuberculosis is an examination of the leukocyte count. This study aimed to determine the description of the number of leukocytes in patients with Suspected SO Pulmonary TB at The Patuh Patut Patju Hospital Untuk. This study design is descriptive case study research. Sampling technique was carried out using accidental sampling technique and  30 respondents obtained. The results of this study showed that the average leukocyte levels of patients with suspected SO Pulmonary TB were 9,643/μl, with 13 people (1+), 12 people (2+), 5 people (3+). The highest average leukocyte count was found in the female gender, age group 56-65 years and on 5 months treatment
Pemberdayaan Kader Posyandu Keluarga di Puskesmas Tanjung Karang sebagai Pelaksana Pemantauan Glukosa Darah Mandiri (PGDM) pada Pasien Diabetes Melitus Kusuma Dewi, Lale Budi; Khusuma, Ari; Manu, Thomas Tandi
Jurnal Indonesia Mengabdi Vol. 5 No. 2 (2023): December Edition
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) STKIP Nurul Huda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30599/jimi.v5i2.2785

Abstract

Pemantauan glukosa darah mandiri dapat dilaksanakan oleh tenaga yang telah mendapatkan edukasi dari tenaga kesehatan terlatih. Kesadaran penderita DM di Kelurahan Tanjung Karang Permai untuk selalu memantau kadar glukosa darahnya seringkali tidak dapat dilaksanakan karena tidak memiliki keterampilan melaksanakan PGDM. Guna memfasilitasi penderita DM agar pelaksanaan PGDM tepat waktu, diperlukan tenaga pelaksana yang dekat dengan masyarakat yaitu kader kesehatan di lingkungan tersebut. Terhadap 20 kader perwakilan dari 7 Posyandu diberikan penyuluhan tentang pentingnya PGDM dan diberikan pelatihan melaksanakan PGDM. Kegiatan penyuluhan dan pelatihan dilaksanakan di Kantor Lurah Tanjung Karang Permai. Dilanjutkan  pendampingan kader untuk PGDM pada Posyandu tempat kader bertugas. Keterampilan kader dalam melaksanakan PGDM dinilai menggunakan daftar tilik evaluasi keterampilan PGDM. Evaluasi didapatkan sebanyak 20 orang kader posyandu di Kelurahan Tanjung Karang Permai sudah memenuhi syarat sebagai pelaksana PGDM.
Hubungan Kadar Ureum dan Kreatinin Dengan Kadar Hemoglobin (Hb) pada Penderita Diabetes Melitus Tipe 2 Widiasih, Ni Kadek; Wiadnya, Ida Bagus Rai; Manu, Thomas Tandi; Kristinawati, Erna
JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 20 No 1 (2025): JPP (Jurnal Kesehatan Poltekkes Palembang)
Publisher : Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jpp.v20i1.2866

Abstract

Background: High blood sugar levels in people with diabetes mellitus will cause blockages and reduce the blood flow rate, thus decreasing the blood supply to the kidneys. This is indicated by high levels of urea and creatinine. Kidney damage leads to decreased production of the hormone erythropoietin, resulting in low oxygen levels and reduced red blood cell production, which can lead to anaemia.. Methods: This research method uses an analytical observation approach with a cross-sectional design. The sampling technique is non-random purposive sampling, Results: The results of the statistical test using the Spearman test showed a P value of 0.00 > 0.05, meaning that Ho is accepted and Ha is rejected. Conclusion: There is no relationship between urea and creatinine levels and haemoglobin (Hb) levels in people with type 2 diabetes mellitus.
Differences In Lipid Profiles In Diabetes Mellitus Patients Based On Cigarette Consumption Fihiruddin, Fihiruddin; Putri, Ananda Kinanti; Manu, Thomas Tandi
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 1 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i1.369

Abstract

Diabetes mellitus (DM) is a metabolic condition characterized by hyperglycemia and abnormalities in protein, lipid and carbohydrate metabolism. Smoking is one thing that can cause DM. Smoking as a risk factor for insulin resistance. An increase in lipid profile is directly related to insulin resistance, which increases blood sugar levels by increasing lipolysis and decreasing lipogenesis in fat cells. To determine differences in lipid profiles (total cholesterol, HDL, LDL, triglycerides) in diabetes mellitus sufferers based on cigarette consumption. This research is Analytical Observational using secondary data with an approach Cross Sectional. The sampling method used in this research was purpossive sampling. Data was collected using medical record data on the lipid profile of 48 samples of diabetes mellitus sufferers who consumed cigarettes and analyzed using statistical tests Paired Sample t-test. Were that the mean levels of total cholesterol, HDL, LDL and triglycerides in diabetes mellitus patients who consumed less than 20 cigarettes per day were 228.8 mg/dL, 49 mg/dL, 127.4 mg/dL and 144.4 mg/dL. The mean levels of total cholesterol, HDL, LDL and triglycerides in diabetes mellitus sufferers who consumed more than 20 cigarettes per day were 185.3 mg/dL, 32.7 mg/dL, 116.7 mg/dL and 148 mg/dL. There is a significant difference between the HDL lipid profile in diabetes mellitus patients who consume less than 20 cigarettes per day and more than 20 cigarettes per day.
Utilization Of Kuku Pancar (Lawsonia Inermis Lin) Leaves as a Substitute For Eosin Dye in The Examination of Soil Transmitted Helminth (STH) Eggs Yusari, Lala Deswinta; Srigede, Lalu; Manu, Thomas Tandi; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.356

Abstract

Nail surfacing leaves (Lawsonia inermis lin) are natural materials commonly used as dyes, because of the content of chemical compounds in them such as lawsone, flavonoids, tannins and anthocyanins, therefore nail surfacing leaves can be used as an alternative dye to replace 2% Eosin in STH worm egg examination, because it has the same properties as 2% Eosin and can produce an orange yellow color after the extraction process. The research objective is to determine the results of nail surfacing leaves (Lawsonia inermis lin) can be used as an alternative substance to replace Eosin in the examination of STH (soil transmitted helminth) worm eggs. The methods of this research is using is experimental, where this study looks at the clarity of soil transmitted helminth (STH) worm eggs on preparations, using coloring from the results of soaking the leaves of the nail surf as a substitute for Eosin. The results of the research is that the coloring results obtained in each variation of immersion time are good. The conclusion of this research is that the staining of worm eggs using the results of immersion of nail surfacing leaves for 12 hours, 24 hours, 36 hours, and 48 hours can be used as an alternative dye in the examination of STH worm eggs.
Correlation of Leukocyte Esterase Positivity Levels Against the Results of Several Examination Parameters Laboratory Urinary Tract Infection in Patients at Mataram City Regional Hospital Waisnawa, I Made Wahyu; Wiadnya, Ida Bagus Rai; Manu, Thomas Tandi; Agrijanti, Agrijanti
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.326

Abstract

Background : Urinary tract infection (UTI) is caused by the multiplication of bacteria in the urinary tract. The standard proving procedures for UTI are urine culture examination and urine dipstick examination (leukocyte esterase and nitrite). Objective : This study aims to measure leukocyte and nitrite values, calculate the total number of bacteria, identify Gram type, species and antibiotic sensitivity and analyze the correlation of leukocyte esterase positivity to the results of several UTI examination parameters. Research Methods : The method used is an analytic observational design with cross sectional design using primary data and analyzed with the Spearman rank test. Results : The results showed that in 198 patients with suspected UTI, 123 (62%) were positive for leukocyte esterase, 16 (8.1%) were positive for nitrite. The total number of bacterial countshowed 82 people (41%) with bacteriuria ≥10 5 CFU/mL. Found 61 Gram-negative bacilli and 21 Gram-positive cocci with 21 different bacterial species. Antibiotic sensitivity test showed 51% sensitive, 6% intermediate and 43% resistant. Correlation of leukocyte esterase with nitrites (p 0.023<0.05), total bacteria count (p 0.00<0.05), Gram negative bacilli (p 0.00<0.05), and Gram positive cocci (p 0.533>0.05), antibiotic sensitivity test (p 0.794 >0.05) and bacterial species (p 0.316>0.05). Conclusion : The results showed that there was a correlation between the level of positivity of leukocyte esterase and nitrite, total bacteria count and Gram-negative bacilli, but there was no correlation between Gram-positive cocci, bacterial species and antibiotic sensitivity tests.
Correlation Of Positive IgM or IgG RDT (Rapid Diagnostic Test) Results with CRP (C-Reactive Protein) Levels in Dengue Hemorrhagic Fever Patients Salsabila, Yaumi Tasu’a; Ariami, Pancawati; Wiadnya, Ida Bagus Rai; Manu, Thomas Tandi
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.319

Abstract

Background: Laboratory diagnosis of Dengue Hemorrhagic Fever includes the IgM & IgG RDT (Rapid Diagnostic Test) examination which differentiates primary and secondary dengue infection. The presence of inflammation in the body caused by the dengue virus needs to be examined for C-Reactive Protein. Methods: This research is an analytical observational study with a cross sectional approach. Thesample used is a plasma sample of a patient diagnosed with DHF with a positive IgM or IgG RDT result. Results: Positive IgM RDT results were 6 people (17%) and positive IgG were 30 people (83%). The results of a qualitative CRP study were 24 people (67%) with positive CRP levels. Semi-quantitative CRP average level for positive IgG was 29.33. The results of the Spearman's Rank statistical test obtained a value of p = 0.003 <0.05 which showed a significant result. Conclusion:Clinically it shows that the more positive IgM or IgG RDT results obtained, the higher the CRP level which indicates the severity/inflammation that is formed in the body.
The Effect Of Duration Of Tuak Consumption On Low Density Lipoprotein (LDL) And Triglyceride (TG) Levels In The Community In Taman Bali Village Manu, Thomas Tandi; Ananda, Dewa Ayu Putu Wiweka; Getas, I Wayan; Wiadnya, Ida Bagus Rai; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.544

Abstract

Tuak is a traditional alcoholic drink made from palm sap. Excessive alcohol consumption over a long period of time can affect lipid metabolism, increasing LDL and Triglyceride levels in the blood. Increased levels can cause cardiovascular disease. To determine the effect of the duration of tuak consumption on LDL and Triglyceride levels in the community in Dusun Taman Bali. The research used is Observational Analytical with a cross-sectional approach. The sample in this study was the community in Dusun Taman Bali who consumed Tuak for 1, 3, and 5 years with a total of 30 respondents using One Way Anova data analysis. The average LDL levels of 30 respondents with an average of 117.20 mg/dL consumed tuak for 1 year, 130.30 mg/dL for 3 years and 151.20 mg/dL for 5 years, while the average Triglyceride levels of 30 respondents who consumed Tuak for 1 year were 160.90 mg/dL, 166.60 mg/dL for 3 years and 201.10 mg/dL for 5 years. The results obtained showed a significant value of LDL levels of 0.000 while Triglyceride levels were 0.022. This means P <0.05, which means that there is an effect of the duration of tuak consumption of 1.3 and 5 years on LDL and Triglyceride levels in the community in Dusun Taman Bali.
Identifikasi Sel Ragi (Candida sp.) pada Sedimen Urine dengan Menggunaka Metode Preparat Basah dan Preparat Gram pada Penderita Diabetes Melitus Cahayaningrum, Aryani; Agrijanti; Manu, Thomas Tandi; Khusuma, Ari
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 1 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i1.75

Abstract

Latar Belakang: Kandidiasis adalah infeksi jamur yang disebabkan oleh jamur Candida sp. Diabetes melitus menjadi salah satu faktor predisposisi terjadinya infeksi jamur. Diagnosa dari kandidiasis bisa dilakukan dengan berbagai macam cara. Salah satunya adalah dengan melihat sel ragi pada sedimen urin di bawah mikroskop dengan menggunakan metode preparat basah atau melakukan pewarnaan gram . Tujuan Penelitian: Mengidentifikasi sel ragi (Candida sp.) pada sedimen urin dengan menggunakan metode preparat basah dan preparat gram pada penderita Diabetes Melitus. Metode Penelitian: Rancangan Penelitian ini merupakan penelitian deskriptif, yang dilakukan dengan metode observasional analitik dengan pendekatan cross sectional sehingga variabel bebas dan variabel terikat diamati dalam satu waktu. Hasil: Didapatkan 8 sampel positif sel ragi dan 14 sampel negatif sel ragi pada metode preparat basah dan preparat gram. Kesimpulan: Berdasarkan kualitas, preparat gram lebih unggul daripada preparat basah karena sel ragi yang terlihat pada preparat gram lebih jelas daripada sel ragi yang terlihat pada preparat gram. Sedangkan dari segi efisensi waktu dan biaya, preparat basah lebih unggul karena cepat dan murah.
Co-Authors Agrijanti, Agrijanti Ananda, Dewa Ayu Putu Wiweka Cahayaningrum, Aryani Dewi, Lale Budi Kusuma Fihiruddin, Fihiruddin Getas, I Wayan Khusuma, Ari Kristinawati, Erna Lalu Srigede, Lalu Pancawati Ariami Putri, Ananda Kinanti RAI WIADNYA, IDA BAGUS Reginata, Ni Luh Vira Rohmi, Rohmi Salsabila, Yaumi Tasu’a Waisnawa, I Made Wahyu Widiasih, Ni Kadek Yusari, Lala Deswinta