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All Journal Jurnal Kesehatan Andalas Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Medicine Jurnal Pengabdian Magister Pendidikan IPA Jurnal Analis Medika Biosains (JAMBS) Hydrogen: Jurnal Kependidikan Kimia
Lalu Srigede, Lalu
Jurusan Analis Kesehatan Poltekkes Kemenkes Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

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Edukasi, Pelatihan Dan Pemberdayaan Kader Kesahatan Posyandu Cakranegara Utara Tentang Skrining Rapid Dbd Ns-1 Resnhaleksmana, Ersandhi; Srigede, Lalu; Getas, I Wayan
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 4 (2023): Oktober-Desember 2023
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v6i4.6218

Abstract

Handling infectious diseases, such as Dengue Hemorrhagic Fever (DHF), will be successful if it involves all levels of society. One of the spearheads of health service activities is posyandu cadres. Posyandu cadres' knowledge regarding infectious diseases, especially Dengue Hemorrhagic Fever (DHF), is still lacking, and it is known that the viral infectious disease that remains a scourge on health is dengue hemorrhagic fever. Dengue Hemorrhagic Fever (DHF) often causes health problems for the community and often causes outbreaks of Extraordinary Events (KLB) with large numbers of deaths. Techniques that can be used are education about Dengue and training of mosquito larvae monitors for cadres at Community Health Centers. The aim of carrying out this activity is to increase cadres' knowledge of Dengue Hemorrhagic Fever and the Rapid Test for Dengue Dengue NS-1. The target of this activity is the North Cakranegara posyandu cadre group. The results of the outreach and training showed an increase in cadres' knowledge and skills regarding the prevention and use of the NS-1 Dengue Rapid Test.
Cloning, expression and purification of a gene encoding CFP-10 and ESAT-6 protein of Mycobacterium tuberculosis in pET SUMO plasmids as Vaccine Candidates Fihiruddin, Fihiruddin; Inayati, Nurul; Srigede, Lalu; Zaetun, Siti; Unsunnhidal, Lalu; Shaleh, Muhammad Nazil
Jurnal Penelitian Pendidikan IPA Vol 12 No 2 (2026): In Progress
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i2.12724

Abstract

The Bacillus Calmette-Guerin vaccine did not show consistent results in preventing tuberculosis, with an effectiveness ranging from 0% to 80%. More effective proteins are needed as vaccine candidates to eliminate tuberculosis. Culture filtrate proteins 10-kDa(CFP-10) and Excretory Antigen Target 6-kDa(ESAT-6) demonstrated very strong antigenicity against T and B cells. These proteins are secreted during the early phase of infection and could potentially be used for vaccine candidates. This study aims to clone and express the CFP-10 and ESAT-6 proteins on the pET SUMO Plasmid. PCR with specific primers was used to amplify the genes encoding CFP-10 and ESAT-6 proteins. The PCR products were cloned into pET SUMO plasmids for transformation into competent cells E.coli BL21(DE3). Recombinant protein expression was induced in LB medium using 1 mM IPTG. The yield of recombinant proteins was visualized by SDS-PAGE and western blotting using an Anti-HisTaq Antibody. PCR results showed the target gene sizes to be 304 bp (CFP-10) and 291 bp (ESAT-6). SDS-PAGE and Western blotting revealed proteins at 22kDa (CFP-10) and 18kDa (ESAT-6). The genes encoding the CFP-10 and ESAT-6 proteins of M. tuberculosis have been successfully cloned into the pET SUMO plasmid and expressed in E. coli BL21(DE3) with molecular weights corresponding to the target genes.
Co-Authors Agrijanti, Agrijanti Ari Khusuma Asyhaer, Rifki Khalidi Dektra, I Made Putra Pramaditya Dewi, Lale Budi Kusuma Fihiruddin, Fihiruddin Getas, I Wayan kristinawati, Erna Nurul Inayati Octavira, Imanta Alifia Oktaviani, Andi Dwi Pauzi, Iswari RAI WIADNYA, IDA BAGUS Resnhaleksmana, Ersandhi Rohmi, Rohmi Sani, Syahrul Shaleh, Muhammad Nazil Siti Zaetun, Siti Thomas Tandi Manu Unsunnhidal, Lalu Wadnya, Ida Bagus Rai Wahyudi, Nizar Wahyuni, Leni Wiadnya, Ida Bagus Yunan Jiwintarum Yusari, Lala Deswinta