Irnamanda D.H., Irnamanda
Universitas Lambung Mangkurat

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PHYTOCHEMICAL AND CYTOTOXICITY TESTING OF RAMANIA LEAVES (Bouea macrophylla Griffith) ETHANOL EXTRACT TOWARD VERO CELLS USING MTT ASSAY METHOD (Preliminary study of adjuvant therapy materials to the preparation of the drug) Fitri, Laila; Taufiqurrahman, Irham; DH, Irnamanda
Dentino Vol 3, No 1 (2018)
Publisher : FKG Unlam

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Abstract

Background: Ramania leaves (Bouea macrophylla Griffith) are one of the local medicinal plants of South Kalimantan which is used as adjuvant therapy material. Previous research showed that ramania leaves ethanol extract showed that the extract has potential as adjuvant therapy material for anticancer, but the safety of this material is not yet known so it  needs to be tested with cytotoxicity test to know the toxic properties of the material toward normal cells in vitro. Objective: This research aims to determine the cytotoxicity properties of ramania leaves ethanol extract toward Vero cells using MTT assay method. Method: It was a true experimental research using posttest-only with control group design consist of 8 treatment groups: 31.25μg/mL, 62.5μg/mL, 125μg/mL, 250μg/mL, 500μg/mL, 1000μg/mL, 2000μg/mL and 4000μg/mL and 2 control groups which were cell control and media control. Study phases include phytochemical test, tannic acid test, cell culture, harvest and cell calculation and cytotoxicity test. The result test was read by ELISA reader, the absorbance will be calculated  by a  formula. Results: The test result showed that ramania leaves ethanol extract contained secondary metabolite such as phenol, flavonoid, steroid and terpenoid. The identification test of tannic acid compound  using TLC method (thin layer chromatography) showed the presence of tannic acid compound.The probit analysis showed IC50in 35.808μg/mL. Conclusion: Based on the research, it can be concluded that ramania leaves ethanol extract are found cytotoxic toward Vero cells after the analysis using MTT assay method (IC50<100μg/mL).
PERBANDINGAN KARAKTERISTIK SHOVEL SHAPE GIGI INSISIVUS PERTAMA RAHANG ATAS PADA SUKU DI KALIMANTAN (Studi Identifikasi Morfologi Gigi antara Suku Dayak Bukit, Suku Dayak Ngaju dan Suku Banjar Hulu) D. Destiarini, Siti Rachmatiara; DH, Irnamanda; Aflanie, Iwan
Dentino Vol 2, No 2 (2017)
Publisher : FKG Unlam

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Backgrounds: Borneo island is inhabited by various tribes such as the Dayak Bukit, Dayak Ngaju and Banjar Hulu. According to Radam (1987) and Tjilik Riwut (1979) these tribes have similarities based on theory of its origins, so the role of identification is required to distinguish people between the invidual in these tribes. Primary identification using dental morphology,one of themis shovel shape which is shown on palatal surface of incisors. Purpose: The research aimed to compare the characteristics of shovel shape maxillary first incisors among some tribes in Borneo. Methods: This research is an analytic observational study with cross sectional design, using dental casts of 156 people that consisting of 52 people Dayak Bukit, 52 people of Dayak Ngaju, and 52 people of Banjar Hulu. Shovel shape of maxillary first incisors observed with score created by Herdlicka (1920). Results: The results showed that the most characteristic shape shovel on Dayak Bukit and Dayak Ngaju are 2 (semi shovel) and Banjar Hulu is 1 (trace shovel). Data were analyzed using Chi-Square test with p=0,0001(p <0.05) indicate a difference in the shovel shape of maxillary first incisors among Dayak Bukit, Dayak Ngaju Dayak, and Banjar Hulu, then performed the Chi-Square test on each of two tribes to describe the comparison between tribes which have differences. Conclusion: Based on the comparison of shape shovel can be concluded there is a possibility of kinship Dayak Bukit with Dayak Ngaju.
THE OVERVIEW OF MAXILLARY AND MANDIBULARY MESIODISTAL TEETH SIZE OF MEN AND WOMEN ON BANJARNESE PEOPLE DH, Irnamanda; Wibowo, Diana; Malinda. M, Okky
Dentino Vol 2, No 1 (2017)
Publisher : FKG Unlam

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ABSTRACTBackground: Malocclusion is a deviation from normal occlusion therefore, it needs an orthodontic treatment plan. Mesiodistal tooth widths can help provide information before the treatment in dentistry. Variation of dental mesiodistal width is caused by factors of race, genetics, ethnicity, gender, environmental and disease. Purpose: This research aimed at geting an overview of dental mesiodistal size of the maxilla and mandibula in men and women Banjarnese population. Methods: This research was a descriptive using a cross-sectional design. The sampling method used purposive sampling technique. The samples amounted to 40 women and 40 men in banjarnese population with age range between 17 - 22 years. Measurement dental mesiodistal size was done by using digital caliper. Result: The result showed the average size of dental mesiodistal maxilla and mandibular in men was larger than size in women of Banjarnese population. Conclusion: It can be concluded that the average size dental mesiodistal maxilla and mandible in men was larger than size in women of Banjarnese population.Keywords: Dental mesiodistal size, banjarnese population
THE EFFECT OF BAGASSE FIBER ADDITION IN FLEXURAL STRENGTH OF BULK FILL COMPOSITE RESIN Ananda, Siti Farida Rizki; Erlita, Isyana; DH, Irnamanda
Dentino Vol 3, No 1 (2018)
Publisher : FKG Unlam

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Background: Bulk fill composite resin is a packable composite resin that has been modified to solve the deficiency of conventional composite resin. The addition of fiber can increase the mechanical properties, one of them is flexural strength. Fiber is consisted of two types which are synthetic fiber and natural fiber. In Indonesia, there are many natural fibers, one of them is from the plant of sugarcane. The baggase is the residual from sugarcane plant that had been processed for the making of sugar. Purpose: To acknowledge if the addition of baggase fiber affect the flexural strength of bulk fill composite resin. Methode: This study wastrue experimental studywith post-test only control group design and used simple random sampling that consisted of 3 groups, which are group with addition of baggase fiber, group without addition of baggase fiber as the negative control and group with addition of synthetic fiber as the positive control. Flexural strength tested with Universal Testing Machine. Result: The average value of flexural strength on group with addition of baggase fiber, group without addition of baggase fiber and group with addition of synthetic fiber were 123,549 MPa; 118,125 MPa and 144,442 Mpa respectively. One Way Anova and Post Hoc Bonferroni test showed that there is significant difference between all treatment groups. Conclusion: Based on this study, it can be concluded that addition of baggase fiber can increase the flexural strength but cannot replace the synthetic fiber.
THE EFFECT OF ACIDITY LEVEL AND SUBMERSION DURATION OF TEETH IN PEATLANDS TO DETERMINE BLOOD-GROUP ACCURACY THROUGH DENTAL PULP Study of postmortem blood-group identification technique through dental pulp DH, Irnamanda; Aflanie, Iwan; Raban, Meka Aulia; Habibah, Ainul
Dentino Vol 2, No 2 (2017)
Publisher : FKG Unlam

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Background: Indonesia has peatlands which spread all over the state and one of them is in Borneo(Kalimantan). More than three million hectares of peatland spreads in South Borneo. Peatlands have relativelyhigh acidity level with pH range of 3-5. The peatlands in South Borneo is generally used for farming or publiccemetery. In certain situation, peatlands is often used as a dumping ground for criminal victims. Sometimes, theauthority finds it hard to identify the victim because the body is already decomposed. To identify the victim andto analyse the cause of death, identification process is necessary. Teeth can be used to help the identificationprocess. Biological elements from the teeth namely dental pulp contains antigens that were useful to bloodgroupsdetermination by absorption elution method. Purpose: The objective of this research is to discover theeffect of peatlands acidity level and teeth submersions durations in determining blood-group accuracy fromdental pulp. Method: The method of this research used a quasi-experimental method to discover the effect ofpeatlands acidity and pre-experimental method to discover the effect of teeth submersions duration. Thisresearch used 48 pieces premolar teeth that were divided into 8 groups, control group, group submerged onpeatlands with pH 3,0-3,9, pH 4,0-4,9 and pH 5,0-5,9 to discover the effect of peatlands acidity and 1-day, 3-day, 5-day and 7-day groups to discover the effect of teeth submersions time. Result: Fishers Exact test resultsshowed p value 0,314 (p > 0,05) for the effect of peatlands acidity and p value 0,410 (p > 0,05) for the effect ofteeth submersions duration. Conclusion: It could be concluded that there are no effect of the peatlands acidityand teeth submersions time to determine blood-group accuracy from dental pulp.
THE DIFFERENCES OF HEAD SHAPE CHARACTERISTICS IN KALIMANTAN TRIBES USING FRONTOPARIETAL INDEX Zahroh, Khanifatu; DH, Irnamanda; Aflanie, Iwan
Dentino Vol 3, No 1 (2018)
Publisher : FKG Unlam

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Backgrounds: Kalimantan is the largest island in Indonesia that consists of 5 provinces, including South Kalimantan and Central Kalimantan. South Kalimantan and Central Kalimantan are inhabited by several tribes such as Dayak Bukit tribe, Banjar Hulu tribe and DayakNgajutribe. The geographic condition of inter-ethnic residence affects the phenotype pattern that will characterize each population. Those characteristics can be measured using the frontoparietal index that are classified into 3 characteristic forms, which are <68.9% (Sthenomethopia/narrow forehead), 69.0-70.9% (Metriometopia/moderate forehead) and >71,0 (eurymetopia/wide forehead). Objective: To know the difference in head shape characteristicsusing frontoparietal index betweenDayak Bukit tribe, Banjar Hulu tribe and DayakNgaju tribe. Methods: This research was an analytic observational study with cross sectional design,using 180 people that consisted of 60 people from Dayak Bukit tribe, 60 people from DayakNgaju tribe, and 60 people from Banjar Hulu tribe which were measured by using digital caliper. Results: Measurements showed that the mean of frontoparietal index were 89,20% (Eurymetopia) in Dayak Bukit tribe, 88,77% (Eurymetopia) in BanjarHulu tribe, and 89,63% (Eurymetopia) in DayakNgaju tribe. The results of the research analyzed by Chi Square test with p=0,36 (p>0,05). It  showed thatthere was no difference in head shape characteristics using frontoparietal index between Dayak Bukit tribe, Banjar Hulu tribe and DayakNgaju tribe. Conclusion: Frontoparietal index can not be used to differentiate the head shape characteristics of Dayak Bukit tribe, Banjar Hulu tribe and DayakNgaju tribe.
PERBANDINGAN RERATA BESARAN LEEWAY SPACE SUKU BANJAR DENGAN RERATA LEEWAY SPACE MENURUT PROFFIT (Studi Analitik Observasional Radiograf! Periapikal pada Masa Gigi Bercampur) Gusti Meidy L; Fajar Kusuma D.K; Irnamanda D.H.
ODONTO : Dental Journal Vol 3, No 1 (2016): July 2016
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.3.1.20-26

Abstract

Background: Leeway space is an excess space available because of the differences between the amount of mesiodistal width of canine, first premolar and second premolar and the amount of mesiodistal width of primary canine, first molar and second molar. There's a variation of Leeway space among individuals. This variation occurs because of growth and development period which are influenced by environmental factors, nutrition and ethnic variations. Purpose: The purpose of this study was to determine the difference in the average Leeway space of Banjarese population with the size of Leeway space according to Proffit.Method: This study used an observational analytic with cross sectional approach. The number of mesiodistal width of primary c, m1, and m2 is substracted with the number of mesiodistal width of permanent C, P1, and P2 of 77 samples to determine the average Leeway space and then compared with the Leeway space according to Proffit. Measurements were done using a digital caliper followed with data analysis using Mann-Whitney test.Result: The average of Banjarese population Leeway space is 0.37 mm in each region of the maxilla and 2.2 mm in each region of the mandible. Results of the Mann-Whitney test showed that there were significant differences in maxillary Leeway space of Banjarese population compared to the Leeway space according to Proffit.Conclusion: The conclusion of this study is there were differences between the average Leeway space of Banjarese population with the Leeway space according to Proffit.
PHYTOCHEMICAL AND CYTOTOXICITY TESTING OF RAMANIA LEAVES (Bouea macrophylla Griffith) ETHANOL EXTRACT TOWARD VERO CELLS USING MTT ASSAY METHOD (Preliminary study of adjuvant therapy materials to the preparation of the drug) Laila Fitri; Irham Taufiqurrahman; Irnamanda DH
Dentino : Jurnal Kedokteran Gigi Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i1.4617

Abstract

Background: Ramania leaves (Bouea macrophylla Griffith) are one of the local medicinal plants of South Kalimantan which is used as adjuvant therapy material. Previous research showed that ramania leaves ethanol extract showed that the extract has potential as adjuvant therapy material for anticancer, but the safety of this material is not yet known so it  needs to be tested with cytotoxicity test to know the toxic properties of the material toward normal cells in vitro. Objective: This research aims to determine the cytotoxicity properties of ramania leaves ethanol extract toward Vero cells using MTT assay method. Method: It was a true experimental research using posttest-only with control group design consist of 8 treatment groups: 31.25μg/mL, 62.5μg/mL, 125μg/mL, 250μg/mL, 500μg/mL, 1000μg/mL, 2000μg/mL and 4000μg/mL and 2 control groups which were cell control and media control. Study phases include phytochemical test, tannic acid test, cell culture, harvest and cell calculation and cytotoxicity test. The result test was read by ELISA reader, the absorbance will be calculated  by a  formula. Results: The test result showed that ramania leaves ethanol extract contained secondary metabolite such as phenol, flavonoid, steroid and terpenoid. The identification test of tannic acid compound  using TLC method (thin layer chromatography) showed the presence of tannic acid compound.The probit analysis showed IC50in 35.808μg/mL. Conclusion: Based on the research, it can be concluded that ramania leaves ethanol extract are found cytotoxic toward Vero cells after the analysis using MTT assay method (IC50<100μg/mL).
PERBANDINGAN KARAKTERISTIK SHOVEL SHAPE GIGI INSISIVUS PERTAMA RAHANG ATAS PADA SUKU DI KALIMANTAN (Studi Identifikasi Morfologi Gigi antara Suku Dayak Bukit, Suku Dayak Ngaju dan Suku Banjar Hulu) Siti Rachmatiara D. Destiarini; Irnamanda DH; Iwan Aflanie
Dentino : Jurnal Kedokteran Gigi Vol 2, No 2 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v2i2.3999

Abstract

Backgrounds: Borneo island is inhabited by various tribes such as the Dayak Bukit, Dayak Ngaju and Banjar Hulu. According to Radam (1987) and Tjilik Riwut (1979) these tribes have similarities based on theory of its origins, so the role of identification is required to distinguish people between the invidual in these tribes. Primary identification using dental morphology,one of themis shovel shape which is shown on palatal surface of incisors. Purpose: The research aimed to compare the characteristics of shovel shape maxillary first incisors among some tribes in Borneo. Methods: This research is an analytic observational study with cross sectional design, using dental casts of 156 people that consisting of 52 people Dayak Bukit, 52 people of Dayak Ngaju, and 52 people of Banjar Hulu. Shovel shape of maxillary first incisors observed with score created by Herdlicka (1920). Results: The results showed that the most characteristic shape shovel on Dayak Bukit and Dayak Ngaju are 2 (semi shovel) and Banjar Hulu is 1 (trace shovel). Data were analyzed using Chi-Square test with p=0,0001(p <0.05) indicate a difference in the shovel shape of maxillary first incisors among Dayak Bukit, Dayak Ngaju Dayak, and Banjar Hulu, then performed the Chi-Square test on each of two tribes to describe the comparison between tribes which have differences. Conclusion: Based on the comparison of shape shovel can be concluded there is a possibility of kinship Dayak Bukit with Dayak Ngaju.
THE OVERVIEW OF MAXILLARY AND MANDIBULARY MESIODISTAL TEETH SIZE OF MEN AND WOMEN ON BANJARNESE PEOPLE Irnamanda DH; Diana Wibowo; Okky Malinda. M
Dentino : Jurnal Kedokteran Gigi Vol 2, No 1 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v2i1.2593

Abstract

ABSTRACTBackground: Malocclusion is a deviation from normal occlusion therefore, it needs an orthodontic treatment plan. Mesiodistal tooth widths can help provide information before the treatment in dentistry. Variation of dental mesiodistal width is caused by factors of race, genetics, ethnicity, gender, environmental and disease. Purpose: This research aimed at geting an overview of dental mesiodistal size of the maxilla and mandibula in men and women Banjarnese population. Methods: This research was a descriptive using a cross-sectional design. The sampling method used purposive sampling technique. The samples amounted to 40 women and 40 men in banjarnese population with age range between 17 - 22 years. Measurement dental mesiodistal size was done by using digital caliper. Result: The result showed the average size of dental mesiodistal maxilla and mandibular in men was larger than size in women of Banjarnese population. Conclusion: It can be concluded that the average size dental mesiodistal maxilla and mandible in men was larger than size in women of Banjarnese population.Keywords: Dental mesiodistal size, banjarnese population