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Journal : Journal of Microbial Systematics and Biotechnology

Optimization of growth of oyster mushroom mycelium (Pleurotus sp.) from Tasikmalaya on several kinds of cereal medium Khusnul Khusnul
Journal of Microbial Systematics and Biotechnology Vol 1, No 2 (2019): December 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.998 KB) | DOI: 10.37604/jmsb.v1i2.27

Abstract

Pleurotus sp. or oyster mushroom is one type of edible mushroom that contains essential nutrients for the human body. This mushroom has been widely cultivated in Indonesia, one of which is Pleurotus sp. from Tasikmalaya. The key to the success of oyster mushroom cultivation is the seeds and growing medium or baglog, while the factors that influence the quality of the seeds include the type of medium. This study aims to determine 1) the effect of the type of seed medium from the type of cereals used on the growth of mycelium Pleurotus sp. from Tasikmalaya, 2) determine the type of seed medium that has the best mycelium growth. This study uses an experimental method with a Completely Randomized Design (CRD). The parameters measured include the main parameters, namely the rate of growth of mycelium Pleurotus sp. from Tasikmalaya on the seed medium. Supporting parameters are the quality of mycelium growth in the seed medium, the proximate value of cereals used for the seed medium. The results of the analysis showed that the medium of cereals used as seeds ready for cultivation could affect the quality of the seeds produced. The seedling medium from milled corn has the best growth of mycelium.
Effect of ethanol extract from Karuk leaf (Piper sarmentosum Roxb.) on the growth of Malassezia furfur in vitro Khusnul Khusnul; Asti Kusmayanti; Lia Aulia Rahman; Nuniek I Ratnaningtyas
Journal of Microbial Systematics and Biotechnology Vol 2, No 2 (2020): December 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i2.59

Abstract

In Indonesia, there are numerous therapeutic plants found. Some of the plants used in herbal medicine are Karuk leaf (Piper sarmentosum Roxb.) belong to the Piperaceae family. Karuk leaf has chemical contents such as saponins, polyphenols, flavonoids, and essential oils and many tests are carried out on several bacteria, but testing of fungi is rarely studied. This study aims to determine the ethanol extract activities from karuk leaf in inhibiting the growth of the Malassezia furfur fungus and to determine its minimum inhibition by using the Kirby-Bauer method. The study was conducted by an experimental method of the M. furfur fungus using the Kirby-Bauer method. The ethanol extract from karuk leaf was made in various concentrations and tested on 0.5 McFarland fungus by diffusion on Sabouraud Dextrose Agar (SDA). The results of this analysis showed that the ethanol extract of Karuk leaf could inhibit the M. furfur fungus at a concentration of 30% by 5.3 mm, 40% by 6.6 mm, 50% by 7.6 mm, 60% by 11.3 mm, 70% by 12.5 mm, 80% by 15.6 mm, 90% by 17.4 mm, and 100% by 19.5 mm. Based on the results of the study and the data analysis, it can be concluded that several concentrations of ethanol extract of Karuk leaf affect the growth of M. furfur in vitro.
Effect of Clove essential oil (Syzygium aromaticum) against the growth of dandruff scalps-causing fungal pathogen using Kirby- Bauer method in vitro Khusnul Khusnul; Puput Putuh Anisa; Dewi Peti Virgianti
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.78

Abstract

One of the strongest contributing factors in dandruff scalps is the presence of fungal pathogen or called as Malassezia. Several studies have found a connection between Malassezia and scalp health, one of which is caused by pathogenic fungal infections including Pityrosporum ovale, Microsporum gypseum, and Candida albicans. Clove flowers are known to contain eugenol and its derivative compounds which have antimicrobial, antifungal, antiseptic, and local anesthetic activity. This research aims to determine the effect of clove flower essential oil (Syzygium aromaticum) against some dandruff-causing fungi. The research method was to test the inhibitory power of clove flower essential oil using the diffusion method (Kirby Bauer technique). Samples of pathogenic fungi were cultured on SDA media (Saboroud Dextrose Agar) and given discs that had been given several concentrations of clove flower essential oil. The concentrations used were 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, positive control using 2% ketoconazole and negative control using virgin coconut oil (VCO). The results showed a significant effect between the ability of essential oils to inhibit the growth of some fungi that cause dandruff. The treatment of clove flower essential oil against M. gypseum at the concentration of 10% has an inhibitory ability of 33,05 mm and at the concentration of 100% amounted to 63.95 mm. Meanwhile, the inhibition percentage in P. ovale with a concentration of 10% and 100% of extract were 14,75 mm and 46,15 mm, respectively. The inhibition rate in C. albicans with the concentration of 10% extract and 100% of extract was 13,5 mm and 39,35 mm. The research shows that clove flower essential oil inhibits the growth of several pathogenic fungal in vitro.