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Multibasillary Leprae Lepromatous Leprosy Type Relaps with Erythema Nodosum Leprosum Reaction: Establishing the Diagnosis Ria Margiana
Journal of Global Pharma Technology Volume 12 Issue 01
Publisher : Journal of Global Pharma Technology

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Introduction: Leprosy or Morbus Hansen is a disease caused by infection with Mycobacterium Leprae with manifestations in the skin and peripheral nerves that can cause neuropathy or disability. The case of relapse in leprosy is a condition in which patients who have successfully completed the MDT (multi drug therapy) program with complete and appropriate but there are new symptoms of the disease either during the monitoring period or after. ENL reactions can occur at any time, namely before, during and after MDT therapy. Case: A woman aged 43 years comes to Dermatovenereology clinic with complaints of red bumps accompanied by pain in the face and extremities. On physical examination fromt the facial, superior and inferior limbs appear multiple erythematous nodules with partial hyper pigmented, multiple, indistinct boundaries. Investigation in the form of an AFB test indicates the presence of IB + 3 and IM 0% which previously earned IB +1 IM 2.09%. Histopathological examination revealed an ENL reaction with a red acid-resistant bacillus. The patient is diagnosed with leprosy MB type LL RFT1, 5 years relapsed with ENL Discussion: Relapse or recurrence in leprosy can occur due to a variety of factors, one of which is due to the presence of persistent leprosy bacilli, Becx-Bleumink criteria can be used as a reference for diagnosing relapse that includes clinical features, BTA examination and histopathological features.Keywords: Erythema nodosum leprosum, Leprosy, Relapse.

Morphology-based Selection, Metabolic Profiling and Omics Technology Compared with the Classical Morphology-based Embryo Selection in In-Vitro Fertilization Ria Margiana
Journal of Global Pharma Technology Volume 9 Issue 11
Publisher : Journal of Global Pharma Technology

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Introduction: The medical industry is one of the dynamic industries with frequent innovations and discoveries, which in-vitro fertilization (IVF) technology being the recent change. IVF is an assisted reproductive technology that was invented to treat fertility and genetic problems associated with conception which involves implantation of good quality embryosin to uterus. The success of IVF depends majorly on the embryo selection, the age of the gestational carrier and the cause of infertility. In IVF, more than one embryo can be implanted in uterus, which may result in multiple pregnancies about 30% of embryo resulting from IVF treatments have abnormal chromosome, and this percentage may rise up to 60% with poor embryo selection. This has led to many studies in the last decade on possible methods to replace the conventional morphological evaluations or complement with them. This paper, therefore, aims to assess the performance of morphology-based selection, metabolic profiling, and omics technology in IVF procedure when compared with the modern selection methods. Method: This study provides a review of literatures on the assessment of the effectiveness of the traditional embryo transfer methods when compared with the modern methods. The review was based on Pub Med electronic database. Information on female patient, different intervention models, comparisons and study designs was also provided. Results: Out of the 58 studies that were evaluated, only ten were identified as eligible for the review because they provided substantial reports on morphology assessment, metabolic profiling, and omics technology. The selected articles proved to be significant since they provided a comparative analysis of the models and their outcomes. This review identified that the modern methods had positive results as opposed to the traditional methods that had less positive results. Conclusion: Modern methods have positive results due to their precision compared to morphological technique. The time lapse imaging technique has the highest accuracy, and it complements the morphological method by reducing chances of failure of implantation caused by exposing the embryo to conditions outside the incubator. Results from the other modern method including metabolic profiling, omics technology, whether they had lower pregnancy rates and significant implantation failure or not. From the results, one can judge that morpho kinetics is the best model due to its high precision. On the integration of the time-lapse technique with classical morphology assessment, there was no significant difference between the time-lapse technique and the morphology evaluation. Although morph kinetics provides high-quality results, the traditional methods are continually being used because they provide an observational assessment of the embryo cells. Also, the morphology-based method issued worldwide due to the lack of consensus on the benefits of the novel embryo transfer techniques.Keywords: Morphology-based selection, Metabolic profiling, Omics technology, In-vitro fertilization

The Mechanism in which Sjogren's Syndrome Contributes to The Onset of Erectile Dysfunction Ria Margiana; Azlansyah Azlansyah
Indonesian Andrology and Biomedical Journal Vol. 4 No. 1 (2023): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/iabj.v4i1.43832

Sjogren's Syndrome is an autoimmune disorder characterized by the loss of salivary and lacrimal glands, resulting in dry mouth and eyes. Erectile dysfunction (ED) is a prevalent complaint among people with Sjogren's Disease, however the underlying mpoiechanism is not yet known. The purpose of this article is to examine the mechanism through which Sjogren's Disease causes ED and to identify potential therapies. Using MEDLINE, EMBASE, and the Cochrane Library databases, a systematic review was done. Papers investigating the link between Sjogren's Syndrome and ED published between 1990 and 2021 were included in the study. The review paper discusses a number of potential mechanisms for the association between Sjogren's Disease and ED. They include low levels of testosterone, endothelial dysfunction, autonomic neuropathy, and psychosocial issues. The article also discusses potential treatments for ED caused by Sjogren's Disease, including inhibitors of phosphodiesterase type 5, testosterone replacement therapy, and psychological counseling. The research implies a connection between Sjogren's Disease and ED, although the underlying mechanisms remain unknown. It is necessary to conduct additional studies to better comprehend the relationship and develop targeted remedies.

Evaluating the effectiveness of Thiel embalming solution for preserving cadavers in anatomy and surgical education I Made Rayo Putra Indrawan; Ria Margiana
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 9 No 01 (2025): Qanun Medika Vol 09 No 01 January 2025
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v9i01.21946

Cadavers have played a crucial role in anatomy teaching, owing to their resemblance to the anatomical structures found in living individuals. To optimize the utilization of cadavers, embalming procedures that involve the application of chemical fixative agents are needed. The "soft embalming" approach, which involves various salts as fixating agents, was first developed by Walter Thiel. Although the Thiel solution has demonstrated efficacy in enhancing tissue elasticity, consistency, and color, a study is needed to find the most suitable for embalming with this solution to maximize its utility in the context of anatomy and surgical education. The study results show that the Thiel solution produces the best outcomes for its flexibility, uniformity, and color, which is optimal for anatomical and surgical teaching. The efficacy of Thiel's solution for preserving cadavers for educational intentions is noteworthy for the study of anatomy and surgical procedures. The advantageous characteristics of Thiel embalming include flexibility, consistency, and natural coloring. Nevertheless, it is crucial to consider the restrictions associated with preserving the brain, spinal cord, eye, and musculoskeletal tissues. In conclusion, the utilization of Thiel solution has proven to be an efficient embalming agent for preserving cadavers in the fields of anatomy and surgery education, besides brain, spinal cord, eye, and musculoskeletal systems, should be reconsidered.

Effect of Umbilical Cord-derived Mesenchymal Stem Cell Secretome on Corpus Cavernosum Elastography Results in Patients with Severe Erectile Dysfunction Non-responsive to Sildenafil Pratama, Andi Ahmad Thoriq; Rahardjo, Paulus; Atmaja, M Hidayat Surya; Supardi, Petrus; Margiana, Ria
Global Medical & Health Communication (GMHC) Vol 13, No 1 (2025)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v13i1.14144

Erectile dysfunction is one of the most common male sexual dysfunctions, defined as the inability to get or maintain an erection, and its prevalence is increasing over time. The current treatment, using a phosphodiesterase-5 inhibitor like sildenafil, only offers a temporary solution. Secretome, a bioactive substance derived from mesenchymal stem cells from the umbilical cord, has shown remarkable regenerative capabilities. This study aims to explore the potential of administering umbilical cord-derived mesenchymal stem cell secretome to patients with severe erectile dysfunction and evaluate its impact on the stiffness of the corpus cavernosum using elastography in the flaccid phase. This study employed a pre-experimental design with a pre-post test. It involved seven patients who came to Dr. Soetomo Hospital with severe erectile dysfunction that was unresponsive to sildenafil. Elastography evaluation, including strain and shear wave elastography, was performed before and one month after secretome injection, which was carried out from March to April 2024. Data were analyzed using the paired sample t-test when the data distribution was normal and the Wilcoxon test when the data distribution was not normal. There were statistically significant changes (p<0.05) in all patients who underwent strain and shear wave elastography of the corpus cavernosum before and one month after secretome administration, indicating an improvement in stiffness. In conclusion, intracavernosal administration of umbilical cord-derived mesenchymal stem cell secretome has demonstrated promising results in treating severe erectile dysfunction non-responsive to sildenafil therapy. These results include improved penile tissue in the corpus cavernosum, as evidenced by decreased stiffness on elastography.

Evaluating the effectiveness of Thiel embalming solution for preserving cadavers in anatomy and surgical education Indrawan, I Made Rayo Putra; Margiana, Ria
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 9 No 01 (2025): Qanun Medika Vol 09 No 01 January 2025
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v9i01.21946

Cadavers have played a crucial role in anatomy teaching, owing to their resemblance to the anatomical structures found in living individuals. To optimize the utilization of cadavers, embalming procedures that involve the application of chemical fixative agents are needed. The "soft embalming" approach, which involves various salts as fixating agents, was first developed by Walter Thiel. Although the Thiel solution has demonstrated efficacy in enhancing tissue elasticity, consistency, and color, a study is needed to find the most suitable for embalming with this solution to maximize its utility in the context of anatomy and surgical education. The study results show that the Thiel solution produces the best outcomes for its flexibility, uniformity, and color, which is optimal for anatomical and surgical teaching. The efficacy of Thiel's solution for preserving cadavers for educational intentions is noteworthy for the study of anatomy and surgical procedures. The advantageous characteristics of Thiel embalming include flexibility, consistency, and natural coloring. Nevertheless, it is crucial to consider the restrictions associated with preserving the brain, spinal cord, eye, and musculoskeletal tissues. In conclusion, the utilization of Thiel solution has proven to be an efficient embalming agent for preserving cadavers in the fields of anatomy and surgery education, besides brain, spinal cord, eye, and musculoskeletal systems, should be reconsidered.

Centella asiatica Nanoparticles as Potential Acetylcholinesterase Inhibitor for Cognitive Decline Therapy using Ellman's Method: An in Vitro Study Nathania, Nathania; Kusumaningrum, Selvina Cindy; I'tishom, Reny; Kumalasari, Feranita; Margiana, Ria
Folia Medica Indonesiana Vol. 60, No. 4
Publisher : Folia Medica Indonesiana

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Highlights: 1. This research contributes novel data to current studies by combining the potential of Centella asiatica extract with PEG-400 to develop a therapeutic agent aimed at improving cognitive function through an effective drug delivery system capable of penetrating the blood-brain barrier. 2. The findings of this study revealed that the combination of Centella asiatica extract and PEG-400 at an adequate ratio exhibits great potential as an acetylcholinesterase inhibitor. Abstract Cognitive impairment, caused by neurocognitive changes and neuroinflammation, affects 65.6 million elderly people worldwide and can interfere with their quality of life. Centella asiatica is recognized for its neuroprotective potential due to its active compounds. This study aimed to investigate the acetylcholinesterase inhibitory properties of Centella asiatica as potential therapeutic agents for cognitive decline. Polyethylene glycol 400 (PEG-400) was used to achieve an effective drug delivery system of Centella asiatica extract, facilitating the inhibition of the apoptosis signaling pathway and allowing neuroprotective agents to cross the blood-brain barrier (BBB). This research involved several testing stages, including gas chromatography-mass spectrometry (GC-MS) to identify active compounds (e.g., tryptamine, γ-sitosterol, and β-sitosterol) that contribute to cognitive function improvement. Particle size analysis (PSA) tests were conducted on three formulations of the extract and PEG-400, with ratios of 1:100, 100:1, and 1:1, to determine the optimal formulation for subsequent testing. Scanning electron microscopy (SEM) was utilized to observe the morphology and surface structure of the samples, while Ellman's method was employed to test the ability of acetylcholine (ACh) in improving cognitive abilities. The results subsequently underwent descriptive analysis, particle distribution analysis, analysis of variance (ANOVA), nonparametric tests, image analysis, regression tests, multivariate analysis, and correlation tests. The synthesis demonstrated that the 1:100 formulation produced ideal-sized nanoparticles (5–7 nm), optimal for penetrating the BBB. The PSA and SEM analyses supported this finding by demonstrating homogeneous particle morphology and consistent chemical composition. The in vitro Ellman's assay revealed a high inhibitory rate of 97.63% for the 100:1 formulation. The 1:1 and 1:100 formulations demonstrated a very high effectiveness as acetylcholinesterase inhibitors. The combination of PEG-400 and Centella asiatica extract has great potential as an innovative pharmacological therapy for cognitive decline. However, further research is required to ensure the right dosage and development of the research findings.

ANALYSIS OF PENIS SIZE DIFFERENCES IN PATIENTS WITH SEVERE ERECTILE DYSFUNCTION NON-RESPONSIVE PDE5i WITH MESENCHYMAL STEM CELL SECRETOME FROM UMBILICAL CORD Wibowo, Kristophorus Jonathan; I’tishom, Reny; Soebadi, Mohammad Ayodhia; Atmaja, Muhammad Hidayat Surya; Supardi, Supardi; Margiana, Ria; Amalia, Fatkhurrohmah Leo
Folia Medica Indonesiana Vol. 61, No. 2
Publisher : Folia Medica Indonesiana

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The ineffectiveness of PDE5i[-10pc]AU: Please provide the affiliation of all authors. therapy is indicated by the patient’s unresponsiveness to oral medication, evidenced by insufficient erection after four attempts with the dosage recommended by the manufacturer. Consequently, the application of regenerative therapy may be seen as an effort to stimulate a response or improvement in erectile function, especially in individuals with erectile dysfunction who exhibit no significant progress after utilizing PDE5 inhibitors. Regenerative therapy seeks to repair damaged erectile tissue, with the objective of enhancing erectile function and rehabilitating tissue impacted by existing comorbidities. The secretome denotes the aggregate of chemical compounds and biological factors secreted by cells into the extracellular environment. These secretory factors are essential for various biological processes, including maintaining homeostasis, promoting tissue growth and repair, regulating immune responses, controlling inflammation, stimulating angiogenesis, inducing apoptosis, degrading proteins, facilitating cell adhesion, and organizing the extracellular matrix (ECM). We calculated the sample size using a normal significance level with α = 0.05 and a standard power corresponding to β= 0.20. Metode for this research used a pre–post test design and was conducted from April to May 2024 at the Andrology Clinic of Dr. Soetomo General Academic Hospital, Surabaya, involving a sample of 7 patients with severe ED who were non-responders to PDE5i and had type 2 diabetes mellitus. SPL, FPL, and penile circumference were evaluated before and one month after intracavernosal injection of UC-MSC secretome. This study revealed substantial variations in SPL, FPL, and penile circumference among erectile dysfunction patients who were non-responders to PDE5i, both prior to and following the infusion of mesenchymal stem cell-derived secretome from the umbilical cord. SPL rose by 0.715 cm (p-value: 0.047), FPL was increased by 0.643 cm (p-value: 0.022), and penile circumference was enlarged by 0.50 cm (p-value: 0.014). These enhancements were noted on day 30 post-data collection, It is concluded that intracavernosal secretome UC-MSC therapy for severe PDE5i-nonresponsive ED demonstrates improvement in penile blood flow; however, further studies with longer evaluation periods are still needed to obtain more robust results.

The Success Rate of Cryopreservation and Thawing of Embryos at Day 3 and Day 5 Following Intracytoplasmic Sperm Injection (ICSI) Ria Margiana; Maitra Djiang Wen
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.9327

Cryopreservation, the technique of freezing and thawing embryos, is essential in in vitro fertilization (IVF) treatments to ensure the long-term preservation of embryos for future use. This innovative study evaluates the effectiveness of these procedures conducted at a private hospital in Tangerang, Indonesia, over a period of seven years (2016-2023), involving 18,000 couples. The design research was conducted retrospectively. The study examined embryos that underwent cryopreservation and subsequent thawing, with data collected on the rates of cell division observed on Day 3 and the rates of blastocyst formation on Day 5 after thawing. The study investigated the rates of cellular division on Day 3 and the development of blastocysts on Day 5 following the thawing process. The findings demonstrated a remarkable cleavage rate of 95.24% and a blastocyst rate of 97.22%. The remarkable results underscore the efficacy of the advanced freezing and thawing techniques employed. This discovery is highly significant since there is an urgent requirement to improve the success rates of in vitro fertilization (IVF) for couples facing infertility. It offers a fresh sense of hope and increased opportunities for establishing successful pregnancies with the improvement of cryopreservation techniques.

The Potential of Absorbable Gelatin Sponges as Scaffolds for The Delivery of Platelet-Rich Plasma (PRP) in Peripheral Nerve Regeneration: A Literature Review Khoirul Ima; Ria Margiana
Indonesian Health Journal Vol. 5 No. 3 (2026): Indonesian Health Journal
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/ihj.v5i3.899

Peripheral nerve injury is a major clinical problem that can cause persistent motor dysfunction, sensory impairment, neuropathic pain, muscle atrophy, and gait abnormalities. Although peripheral nerves possess regenerative capacity, functional recovery is often incomplete because axonal growth is slow and strongly influenced by the microenvironment surrounding the injury. This study aimed to evaluate the potential of absorbable gelatin sponge (AGS) as a scaffold for delivering platelet-rich plasma (PRP) in peripheral nerve regeneration. A literature review was conducted using relevant studies retrieved from PubMed, Google Scholar, and Scopus, focusing on publications from 2016–2026 concerning gelatin-based scaffolds, PRP, peripheral nerve injury, regenerative mechanisms, and functional recovery. The reviewed evidence indicated that AGS possessed favorable porosity, biocompatibility, absorption capacity, and biodegradability, enabling it to retain PRP at the injury site and support the sustained release of neurotrophic growth factors. PRP contributed to Schwann cell activity, angiogenesis, axonal growth, remyelination, and inflammatory regulation. Experimental findings also demonstrated improvements in axon diameter, myelin thickness, nerve morphology, electrophysiological responses, and gait parameters following AGS-mediated PRP application. In conclusion, AGS represents a promising PRP delivery scaffold for peripheral nerve regeneration. However, standardized PRP preparation, scaffold dimensions, dosage, application techniques, and clinical trials are required before routine clinical implementation can be recommended.

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